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Stabilitas Laju Fotosintesis dan Produktivitas Rimpang Genotipe Curcuma aeruginosa dan Curcuma zanthorrhiza Djarot Sasongko Hami Seno; Mohamad Rafi; Maria Bintang; Popy Asri Kurniatin; Waras Nurcholis
Indonesian Journal of Agronomy Vol. 48 No. 1 (2020): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Curcuma aeruginosa Roxb. rhizome is an essential herb material with significant antimicrobial, anticancer, and antioxidant activities. Thus, it is important to search for C. aeruginosa plant that has the rhizome yield trait with more excellent stability in changing environmental situations. This research aimed to evaluate the genotype x environment interaction using additive main effects and multiple interaction (AMMI) analysis for photosynthetic rate and rhizome yield of C. aeruginosa genotypes. Twenty clones of C. aeruginosa plus three control varieties of C. zanthorrhiza Roxb. were evaluated in three environments (Bogor, Cianjur, and Sukabumi) using a randomized complete block design with three replicates. The photosynthetic rate and rhizome yield showed significantly (p < 0.01) genotype x environment interaction. The AMMI2 explained 100% variability of the photosynthetic rate and rhizome yield parameters. G2, G10, G12, G13, G14, G16, G17, G19, and G20 were stable genotypes for rhizome yield based on AMMI analyses. Meanwhile, the stable genotype in photosynthetic rate recorded in G4, G5, G6, G8, G11, G13, and G14. Keywords: AMMI, genotypes, multilocation trials, pink and blue ginger, varieties

This research has been held since February to November 2008 in the Research Laboratory and Teaching Farm of KPBI (Koperasi Peternak Babi Indonesia), Cisarua District, Bandung Regency and Nutrition Laboratory, Animal Husbandry Faculty, Padjadjaran University. The purpose of this research is to know the effective dosage of curcumin to give equivalent influence with virginiamycin as growth promotor in pig. This research use completely randomized design consisting of five treatments (Rvm: 50 ppm vi Sauland Sinaga; D.T.H. Sihombing; Maria Bintang; Kartiarso .
Forum Pasca Sarjana Vol. 33 No. 2 (2010): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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This research has been held since February to November 2008 in the Research Laboratory and Teaching Farm of KPBI (Koperasi Peternak Babi Indonesia), Cisarua District, Bandung Regency and Nutrition Laboratory, Animal Husbandry Faculty, Padjadjaran University. The purpose of this research is to know the effective dosage of curcumin to give equivalent influence with virginiamycin as growth promotor in pig. This research use completely randomized design consisting of five treatments (Rvm: 50 ppm virginiamicin, R0: tanpa virginiamicin dan curcumin, R1: 120 ppm curcumin, R2: 160 ppm curcumin, and R3: 200 ppm curcumin), with five treatment. This research used 25 starter period pigs, age 2 months with weight rate of 18 kg and variation coefficient of 6.33%. The result shows that giving curcuminoid as feed additive 160 ppm in pig ration significant by influenced to digestible energy, the rate of passage of feed, body weight gain, feed efficiency and time to slaugter weight. It is concluded that curcumin with dose of 160 ppm in pig ration will be able to replace virginiamycin as growth promotor. Key words: curcumin, virginiamicin, pig

Bacteria are microbes which have an ability to live wherever there is a life. Some of the bacteria are saprophyte and some are parasitic. But most of the bacteria have not been identified or cultured; therefore the benefits are still unknown. Methanogenic bacteria are one of the saprophyte bacteria. These bacteria produces methane, a biogas as an alternative fuel in the future. Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water. The o Ardi Kapahang; Maria Bintang; D.D. Sastraatmadja; Dedy Duryadi Solichin
Forum Pasca Sarjana Vol. 30 No. 1 (2007): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Bacteria are microbes which have an ability to live wherever there is a life. Some of the bacteria are saprophyte and some are parasitic. But most of the bacteria have not been identified or cultured; therefore the benefits are still unknown. Methanogenic bacteria are one of the saprophyte bacteria. These bacteria produces methane, a biogas as an alternative fuel in the future. Most of methanogenic bacteria are uncultured, however a few of them are found in the sewage of coconut water. The objectives of this research are to isolate, characterize and identify the methanogenic bacteria that lived in coconut water. The method of this research was fermentation, analysis characterization, and identification of methanogenic bacteria. First, methanogenic bacteria were isolated from coconut water by fermentation. The samples were from four places in Minahasa, which are Rasi (I), Koka (II), Amurang (III), and Lola (IV) and one place in Bogor (V). Secondly, the methane produced from fermentation was analysed by gas chromatography and the bacteria can be characterized by Bergey’s method. The next step is the identification which was conducted by isolating the DNA, amplifying the DNA by PCR, then sequencing the DNA with BioEdit Sequence Aligment. As the result, high and stable methane was produced in Rasi (I) and Amurang (III). The characteristic of the bacteria are red colony (M) and white colony (P). The shape of the colonies is circles, gram positive, basil shape, mesofile, positive of catalase and citrate, positive of sugar fermentation, gelatin, casein and starch hydrolysis also lived in Nutrient Broth with pH 5.7-6.8. The sequencing of isolate P resulting in nucleotide composition of G 31.25%, C 20.58%, A 27.11% and T 21.04% while isolate M are G 31.34%, C 20.31%, A 27.02% and T 21.32%. The identification of isolate M is equal with Clostridium tyrobutyricum (100 %) and isolate P is very close with Clostridium tyrobutyricum (99 %). Key words: bacteria, methane, coconut water

Pengaruh Pemberian Youghurt Terhadap Pertumbuhan Gigi Tikus Putih (Rattus Norvegicus Galur Wistar) Maria Bintang
Indonesian Chemistry Letter Vol 1 No 1 (2001): Buletin Kimia
Publisher : Department of Chemistry

The purpose of this research is to get information about the effect of yoghurt on the growing teeth of Rat (Rattus norvegicus strain wistaf). This research used 30 male rats, which divided into three groups. Each group consist of 10 rats. One group was one month old and the others were two and three months old. One of the rats' incisor teeth was cut and the rest length of the teeth were measured by sliding ruler. Each group of the rats was divided into two sub groups. One sub group was given 1,O ml of yoghurt, the others were glven 1,5 mi and 2,O ml of yoghurt once a day for 10 days. The other sub group was given aquadest with the same dose as a control group. Everyday the length of the teeth was measured. The results of this research indicated that yoghurt can accelerate the growth of the rat's teeth, but based on the statistical analyzed it showed nosrgnificant difference.

Expression and Characterization of Recombinant β-1,3-Glucanase of Burkholderia cepacia (BiogenCC E76) Expressed in Escherichia coli Expression Systems Tri Puji Priyatno; Fitriani Winangsih; Ifa Manzila; Maria Bintang
Jurnal AgroBiogen Vol 15, No 1 (2019): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n1.2019.p35-44

Burkholderia cepacia (Bcc) BiogenCC E76 isolate is an endophytic bacterium producing cell wall degrading enzyme, glucanase, and antagonistic to fungal pathogens, such as Magnaporte grisea and Colletotrichum gloeosporioides. The glucanase is able to lyse fungal cell walls composed of glucan causing disintegrity of mycelia and fungi fail to infect plants. The purpose of this study was to clone, express, and characterize 48 kDa subunit of β-1,3-glucanase from Bcc isolate BiogenCC E76 using the Escherichia coli expression system. The 1,300 bp of the β-1,3-glucanase gene was constructed using the pET-32b vector in BamHI-HindIII restriction sites to generate the pET-Glu plasmid. The gene was fused with nucleotides sequence encoding Trx-tag, His-tag, and S-tag producing 65 kDa of recombinant β-1,3-glucanase. Gene expression in the construct was controlled by the T7 promoter and Trx-tag start codon through IPTG induction. The recombinant β-1,3-glucanase was then purified and its activities were tested at different pH and temperature conditions. Results showed that E. coli carrying pET-Glu overexpressed a 65 kDa protein in induced culture as a soluble protein that was expressed in periplasm. Purification result of the crude extract of the recombinant protein obtained 27% pure enzymes with a specific activity of 1,207.976 U/mg and purity level of 3.9 fold. This recombinant glucanase demonstrated optimal activity at 40°C and pH 5–7. A deeper study is needed to understand the role of 48 kDa subunit of β-1,3-glucanase has in antagonistic mechanism of Bcc against pathogenic fungi.

KONTAMINAN Enterobacter sakazakii PADA SUSU FORMULA BAYI DAN PENGENDALIANNYA nFN Misgiyarta; Maria Bintang
Buletin Teknologi Pasca Panen Vol 7, No 1 (2011): Buletin Teknologi Pascapanen Pertanian
Publisher : Buletin Teknologi Pasca Panen

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Mutu dan keamanan pangan merupakan satu parameter terpenting pada produksi pangan, termasuk susu formula bayi. Susu formula bayi memerlukan standar mutu dan keamanan yang tinggi. Kontaminasi Enterobacter sakazakii pada susu formula bayi mengancam kesehatan dan jiwa bayi. Penanganan kontaminasi E. sakazakii pada susu formula bayi sangat penting dilakukan. Penanganan tersebut antara lain (a) pengendalian proses produksi dengan penerapan higienitas yang tinggi dan penerapan sistem Hazard Analytical Critical Control Point (HACCP) yang ketat, (b) edukasi cara penyajian produk untuk dikonsumsi bayi secara benar di rumah sakit maupun di rumah tangga. Penanganan kontaminasi E. sakazakii pada produk susu formula bayi dengan benar menekan tingkat kematian bayi akibat infeksi E. sakazakii patogen.

Isolasi dan Karakterisasi Bakteri Endofit dari Tanaman Binahong dan Katepeng China Desriani Desriani; Ukhradia Maharaniq Safira; Maria Bintang; Akhmad Rivai; Puspita Lisdiyanti
Jurnal Kesehatan Andalas Vol 3, No 2 (2014)
Publisher : Fakultas Kedokteran, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jka.v3i2.33

AbstrakBakteri endofit merupakan mikroorganisme menguntungkan yang berinteraksi dengan tanaman inang tanpa menyebabkan gangguan atau kerusakan pada tanaman.Beberapa studi menunjukkan bahwa bakteri endofit tertentu dapat memproduksi senyawa kimia yang memiliki efek bagi kesehatan, terutama bakteri endofit yang diisolasi dari tanaman obat.Binahong (Anredera cordifolia) dan Ketepeng Cina (Cassia alata) merupakan tanaman obat yang telah diketahui memiliki beberapa khasiat.Tujuan dari penelitian ini ialah mengisolasi dan mengkarakterisasi bakteri endofit dari kedua tanaman tersebut melalui uji antibakteriterhadap tiga jenis bakteri patogen manusia (Pseudomonas aeruginosa, Staphylococcus aureus, dan Bacillus cereus). Metode: Jumlah bakteri endofit yang berhasil diisolasi dari dua tanaman tersebut berjumlah 73 isolat (37 isolat dari Binahong dan 36 isolat dari Ketepeng Cina). Hasil: Berdasarkan uji antibakteri terhadap tiga bakteri patogen diperoleh 16 isolat bakteri endofit yang memiliki aktivitas potensial (ditandai dengan terbentuknya zona hambat), yaitu 1 isolat dari Ketepeng Cina (terhadap Pseudomonas aeruginosa) dan 15 isolat dari Binahong. Pembahasan: Terbentuknya zona hambat mengindikasikan kemungkinan adanya senyawa kimia yang memiliki efek antibakteri.Kata kunci: bakteri endofit, Anredera cordifolia, Cassia alata, antibakteri.AbstractEndophytic bacteria is a beneficial microorganism that interacts with plant without causing any harm to the host. Most studies showed that certain endophytic bacteria can produce chemical compound which have medical effect, especially endophytic bacteria isolated from medicinal plant. Binahong (Anrederacordifolia) and KetepengCina (Cassia alata) are several of medicinal plants potential to be used for medical purpose. Methods: The objective of this study was to isolate and to characterize endophytic bacteria from both plants through antibacterial activity test against to three kinds of human pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus). Results: total isolated endophytic bacteria from both plants are 73 isolates (37 isolates from Binahong and 36 isolates from KetepengCina). There were 16 isolates have potential activity (indicated by clear zone), one isolate from KetepengCina and 15 isolates from Binahong. Discussion: The formed clear zone indicates antibacterial compounds produced by endophyticbacteriatested. Further research is needed to identify the type of antibacterial compounds produced.Keywords:endophytic bacteria, Anrederacordifolia, Cassia alata, antibacterial.

Aktivitas Invitro Senyawa Antimikroba Streptococcus lactis (INVITRO ACTIVITY ANTIMICROBIAL SUBSTANCE PRODUCED BY STREPTOCOCCUS LACTIS) I Nyoman Suarsana; Maria Bintang; Iwan Harjono Utama; Ni Gusti Agung Ayu Suartini
Jurnal Veteriner Vol 2 No 1 (2001)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Aktivitas Invitro Senyawa Antimikroba Streptococcus lactis (INVITRO ACTIVITY ANTIMICROBIAL SUBSTANCE PRODUCED BY STREPTOCOCCUS LACTIS)

Peningkatan Kandungan Kalium Urin Setelah Pemberian Ekstrak Sari Buah Belimbing Manis (Averrhoa carambola) (THE INCREASE OF POTASSIUM URINE CONTENT AFTER ADMINSTRATION OF CARAMBOLA (AVERRHOA CARAMBOLA) FRUIT JUICE EXTRACT) Ruqiah Ganda Putri Panjaitan; Maria Bintang
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Carambola (Averrhoa carambola L.) has been used as medicinal plant. This research has beenconducted to study the potential diuretic of fruit juice carambola extract on male rats. Diuretic activitywas tested by using Cumming’s method. The treatment was administered only once, and the urine up to 24hours after treatment was collected. The result shows that the administration of 1.6 mL/100 g body weightof fruit juice carambola extract resulted in lower urine volume compared to the without treatment orklortalidon at dose 0.315 mg/100 body weight (p>0.05). Furthermore, Na+ content in treatment rats’ wasurine lower compared to the without treatment or klortalidon (p<0.05). in contrast, high content of K+ wasobserveb in treatment rast’ urine compared to the without treatment or klortalidon (p> 0.05). It is concludedthat the administration of carambola fruit juice extract may increase K+ content in urine and produce moreconcentrated urine. The mechanism of action, however, remains need to be proven, further.

Sintesis Glikogen Hati dan Otot pada Tikus Diabetes yang Diberi Ekstrak Tempe I Nyoman Suarsana; Bambang Pontjo; Tutik Wresdiyati; Maria Bintang
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Glycogen is found at all of body tissue, especially mostly in liver and muscle. The objectives of thisresearch was to evaluate the content of glycogen in liver and muscle of diabetic rats that were treated withextract of tempe. A total of twenty male Spraque Dawley rats of 2 months old were used in this study. Therats were divided into four groups: (1) negative control group (K-), that were not treated extract of tempeand nondiabetic, (2) positif extract of tempe group (ET), that were treated with extract of tempe andnondiabetic (3) positif diabetic group (DM), and (4) diabetic and extract of tempe group (DM+ET). Extractof tempe was orally administered with dose 300 mg/kg bw/day. The treatment was conducted for 28 days.Effect of extract of tempe on body weight of all rats was determined at various time interval at 0, 4, 7, 14,21, and 28 days. At the end of the experiment, all rats were sacrificed by cervical dislocation. Liver andmuscle gastrocnemius were collected for analysis of glycogen level. The result of this study showed thatadministration methanol extract of tempe of 300 mg/kg bw/day can increase body weight, glycogen synthesisin the liver and muscle in normal rats (rat of ET group) and also diabetic rats (rat of DM+ET group). At theend of research, diabetic rats (rat of DM group) were decrease of body weight up to 5.7%. On the rat ofDM+ET group, rat of K(-) group and rat of ET group were increase of body weight of 5.7%, 19.3% and 20.3%,respectively. Glycogen level both liver, and muscle at rat of ET group and rat of DM+ET group wereincrease each of 9.29%, 2.2% in liver and 18.27% and 4.02% in muscle. Glycogen level at rat of DM groupwere decrease up to 42.5% in liver and 31.6% in muscle.

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