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Infeksi Virus Dengue pada Nyamuk Aedes aegypti Menggunakan Artificial Blood Feeding dan Deteksi Virus Dengue Menggunakan Teknik Molekular Endang Srimurni Kusmintarsih; Medina Fadli Latus Syaadah; Edy Riwidiharso; R Tedjo Sasmono
ASPIRATOR - Journal of Vector-borne Disease Studies Vol 11 No 2 (2019): Jurnal Aspirator Volume 11 Nomor 2 2019
Publisher : Loka Litbang Kesehatan Pangandaran

Abstract. Artificial blood-feeding using the parafilm-M membrane can be used as an alternative solution andsubstitute live animals as a source of blood. This method is not only be used for blood-feeding but also to infectthe dengue virus (DENV) to mosquitoes. This study was aimed to determine the effectiveness artificial bloodfeeding using parafilm-M membrane in Aedes mosquitoes originated in Indonesia and determine the positivityof mosquitoes infected by Indonesia DENV-1. DENV-1 was isolated from patient and propagated in Vero cellculture. The feeding was done in cardboard cups after mosquitos have been starved for 4-17 hours before beingfed with human blood. A conical 50ml tube was prepared, and a hole was created in the tube lid. The tubeopening was covered with parafilm. Glycerol was added into conical tube and heated in water bath for an hourat 55oC. A mixture of blood and DENV-1 was made with concentration of 10%. Detection of DENV in bloodfedmosquitos was carried out by using Simplexa Dengue Real-Time RT-PCR assay. The results showed thatthe prevalence of blood-fed mosquitoes reached 66.67% with fasting period for 17 hours. Blood feedingmosquitoes are affected by duration of fasting period, blood-feeding time, and attractants from human skinrubbed into parafilm-M membrane. The prevalence of blood-fed Ae. aegypti infected by DENV was 20.83%.This study provides information on the effectiveness of artificial parafilm membrane blood-feeding in alaboratory setting that will be useful for vector control study in Indonesia.

Keanekaragaman Semut (Hymenoptera: Formicidae) di Kawasan Cagar Alam Bantarbolang Pemalang Jawa Tengah Annisa Fadwa Rhodiyah; Darsono Darsono; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 1 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Cagar Alam Bantarbolang, Pemalang, Jawa Tengah memiliki keanekaragaman yang tinggi baik itu flora maupun faunanya Salah satu indikator kestabilan ekosistem yang baik di suatu ekosistem hutan yaitu adanya keanekaragaman semut. Semut berperan penting dalam ekosistem terestrial sebagai predator, herbivor, detrivor, dan granivor. Semakin tinggi keanekaragaman semut, semakin tinggipula tingkat kesehatan hutan tersebut.Tujuan dari penelitian ini adalah mengetahui keanekaragaman, kemerataan, dan dominansi spesies semutdi Cagar Alam Bantarbolang, Pemalang, Jawa Tengah. Penelitian ini menggunakan metode survey dengan teknik pengambilan sampel perangkap jebak (pitfall trap) denganukuranplot1 m x 1 m sebanyak 8 plot. Pada masing-masing plotdibuat 4 subplot untuk pemasangan pitfall trap yang berisi air gula.Semut diambil dari gelas plastik yang berukuran diameter 7,5 cm dan tinggi 10,5 cm yang ditanam setengah dari tinggi gelas plastik dari permukaan tanah, dipasang setiap minggu selama satu bulan kemudian didokumentasikan dan dilakukan identifikasi. Data yang didapat dianalisis menggunakan indeks keanekaragaman Shannon-Wiener dan Indeks dominansi. Hasil penelitian menunjukan bahwa ditemukan 982 individu yang berasal dari 1 familia yang terbagi menjadi 4 subfamilia. Adapun tingkat keanekaragaman semut dari ordo Hymenoptera dan familia Formicidae yang ada diCagar Alam Bantarbolang, Pemalang, Jawa Tengah menunjukkan nilai indeks keragaman Shannon (H’) berkisar antara 1,394 - 1,855, indeks kemerataan Evenness (E) berkisar antara 0,448 - 0,7101, sedangkan dominasi Simpson (D) yaitu sebesar 0,6363 - 0,8125.

KEANEKARAGAMAN DAN KELIMPAHAN KUPU-KUPU (LEPIDOPTERA : RHOPALOCERA) DI CAGAR ALAM BANTARBOLANG, JAWA TENGAH Ulfah Nuraini; Imam Widhiono; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Kupu-kupu adalah serangga yang termasuk dalam Ordo Lepidoptera, artinya serangga yang hampir seluruh permukaan tubuhnya tertutupi oleh lembaran-lembaran sisik yang memberi corak dan warna sayap kupu-kupu. Keanekaragaman dan Kelimpahan kupu-kupu di pengaruhi oleh faktor lingkungan seperti intensitas cahaya matahari, temperatur, dan kelembapan. Penelitian ini bertujuan untuk mengetahui keanekaragaman dan kelimpahan kupu-kupu di Cagar Alam Bantarbolang, Jawa Tengah. Data yang di dapat di analisis menggunakan indeks keanekaragaman indeks keanekaragaman Shannon-Wiener, indeks kemerataan Shannon-Evenners, dan Indeks dominansi. Hasil penelitian yang telah dilakukan menunjukkan bahwa ditemukan 359 individu dalam 6 familia. Keanekaragaman tertinggi yaitu pada jarak 0 m (H’ = 2.760; E: 0,752). Kelimpahan tertinggi pada jarak 0 m dengan jumlah individu sebanyak 192. Keanekaragaman tertinggi pada jarak 0 m di tepi hutan, sedangkan keragaman terrendah pada jarak 150 m di dalam hutan. Keanekaragaman kupu-kupu didominasi oleh H. glaucippe dari famili Piridae sebanyak 64 individu. Faktor lingkungan yang paling mempengaruhi keanekaragaman dan kelimpahan kupu-kupu adalah intensitas cahaya matahari. Kata kunci: Kupu-kupu, Keanekaragaman, Kelimpahan, Cagar Alam Bantarbolang

Prevalensi dan Variasi Morfometrik Trichodina sp. pada Benih Ikan Gurami (Osphronemus gouramy Lac.) di Desa Rajapolah Tasikmalaya Wildan Mukholladun; Rokhmani Rokhmani; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 3 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.3.3220

Gurami (Osphronemusgouramy Lac.) Is a type of freshwater fish that is widely cultivated by the people of Indonesia. The high price of gouramy is a measure of consumption and the meat is tender and tasty. In Tasikmalaya, carp farmers in the cultivation of gouramy seeds are still experiencing problems. Gouramy seeds raised to adult size experience high mortality due to ectoparasites. One of the ectoparasites was Trichodina sp. Research has been conducted aimed at 1. Knowing the prevalence of gouramy seeds that were attacked by Trichodina sp. obtained from fish farmers in the village of Rajapolah, Tasikmalaya Regency and 2. Knowing the morphometric variations of Trichodina sp. found in gouramy seeds obtained from fish farmers in Rajapolah Village, Tasikmalaya Regency. The method used in this study is a survey method. Seed gourami aged 2 weeks is use as a sample. Isolation and identification were carried out by making a range preparation and painting using a 2% AgNO3 solution. The results found that the prevalence of 88% or high, while the results of the examination of morphometric variations found 4 species of trichodina sp. namely T. Pediculus, T. Nigra, T.heterodentata, and T. Acuta.Keywords:Trichodina sp., Gourami, morphometric variations, abundance, Rajapolah

Prevalensi Infestasi Cacing Gastrointestinal Pada Kambing (Capra sp.) Yang Dipelihara Secara Intensif Dan Semi Intensif Aulia Khirqah; Rokhmani Rokhmani; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 3 No 3 (2021): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2021.3.3.4224

The main purpose of this study are to compare gastrointestinal worm species founds in goats, to compare prevalence and intensity in goats under intensive and semi intensive rearing system and to compare sex-wise and age related prevalence and intensity of gastrointestinal worms in goat. This research was conducted in intensive and semi intensive goat farms in Desa Klareyan, Kecamatan Petarukan, Pemalang using survey method with purposive random sampling technique in November 2020. Gastrointestinal worms species found in goat feces under intensive rearing system are Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., and Ascaris sp. while species founded in goats under semi intensive rearing system are Strongyloides sp., Haemonchus sp., Oesophagostomum sp., Trichostrongylus sp., Ascaris sp., Moniezia expansa, Moniezia benedeni, Fasciola sp., And Schistosoma sp. The intensity of gastrointestinal worm eggs in goat under intensive rearing system was 1184.44±1743,28, its higher than the intensity in goats under semi intensive rearing system which has 1109.50±1573.73 (P> 0.05). The prevalence of gastrointestinal worm infestations in goats under intensive rearing system is 90%, lower than the goats under semi intensive rearing system which has 100% prevalence (Sign. 0.147). Gastrointestinal worms prevalence and intensity in male goats is lower than female goats (Sign. 0,189; P>0,05), while Gastrointestinal worms prevalence and intensity in lamb goat is higher than young goat and adult goat (Sign. 0,241; F<0,05).

Pengaruh iradiasi ultraviolet (254 nm) terhadap pelemahan kemampuan menginfeksi mirasidium Fasciola gigantica Edy Riwidiharso; Billalodin Billalodin
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 3 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.3.167

The controlling of fascioliasis has not been satisfying so far, since the effort of breaking off the fluke life cycle often leads to resistance. The resistance is due to the synchronization between the parasites and their snail host and high infection ability of the parasites enabling them to finish their life cycle. Ultraviolet irradiation provides alternative approach possibly applied to cope with the resistance. The objectives of this research were to know the ability of F. gigantica miracidium in infecting Lymnaea sp. at various ages and to determine time exposure of ultraviolet (254 nm) effectively reducing the ability of miracidium infection. The results indicated that susceptible age of Lymnea javanica is five weeks old, while the most effective time exposure of ultraviolet (254 nm) in weakening the ability of Fasciola gigantica miracidium infection to L. javanica is 60 seconds (P < 0,05). At the moment the study on reinfection effect of F. gigantica miracidium on the immunity of L. javanica is still in progress.

Kelimpahan dan Variasi Morfometrik Trichodina Sp. pada Benih Ikan Gurami (Osphronemus Gouramy Lac.) di Kolam Budidaya Desa Baji Kecematan Kedungbanteng Banyumas Nurrochmah Hastia Sahadian; R Rokhmani; Edy Riwidiharso
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (964.144 KB)

The center one of Gurami fish hatchery is located on Kedungbanteng, Banyumas district, specially on Beji village. Trichodina sp. is a parasites that infecting the seed of gurami fish. Trichodina sp. is found in the seed of gurami fish hatchery center area with the patogenicity level for about 80 %. The gurami hatchery I is vulnerable component, because it is infecting easily by Trichodina sp. The study purposes is for determine Trichodina sp. abundance and morphometric that is infecting the gurami hatchery I at pond cultivated in Beji village. This reseach method is using survey by random sampling. Gurami seed fish sample taken from pond cultivated of gurami hatchery I in Beji village. Every sampling taken 25 fish in four times, so the calculating are 100 fish number. Reseach variable is Trichodina sp. abundance with number and morphometric parameter. That are body, denticle ring, and adhesive disc diameter, also number of denticle and width of Trichodina sp. membrane. The abundance is obtained by calculating the average number of Trichodina sp. with number of infected fish seed. Characteristic of Trichodina sp. morphometric is done by sampling technique from every infected fish seed. Morphometric variation data of Trichodina sp. analyzed descriptively. Result of this research showed that all of gurami seed are infected by Trichodina sp. All of Trichodina sp. are found as many as 5794 individual from the gurami seed infected. The total abundance of Trichodina sp. from gurami seed for about 60,35 individual and it’s relative highly. Number of Trichodina sp. morphometric measurement show the morphometric variation in body diameter in range 60-102,5 μm; 22,5-52,5 μm denticle ring diameter, 40,62-62,5 μm adhesive disc diameter, 20-26 denticle calculating, 2,5-7,5 μm membran width.

Kekayaan Spesies Ektoparasit pada Ikan Brek (Puntius orphoides) Hasil Tangkapan di Sungai Banjaran Purwokerto Banyumas R Rokhmani; Edy Riwidiharso; Prasetyarti Utami
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2019: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.239 KB)

Sungai Banjaran merupakan salah satu sungai yang cukup besar, yang mengalir sepanjang tahun, melewati daerah pedesaan dan perkotaan Purwokerto Kabupaten Banyumas. Sungai Banjaran, yang dimanfaatkan penduduk untuk kepentingan sehari-sehari yaitu untuk mandi-cuci-kasus (MCK) dan untuk membuang sampah. Telah dilakukan penelitian yang bertujuan untuk mengetahui kekayaan spesies Ektoparasit pada ikan Brek hasil tangkapan di sungai Banjaran Purwokerto Banyumas. Metode penelitian ini adalah survei. Prevalensi ektoparasit yang ditemukan pada ikan Brek yang tertangkap di Sungai Banjaran adalah 100%. Jenis ektoparasit yang ditemukan adalah hanya protozoa spesies Ichtyobodosp. Dengan jumlah yang sangat banyak, sampai 20protozoa per-ikan.

Keanekaragaman dan Kelimpahan Kupu-Kupu (Lepidoptera : Rhopalocera) di Cagar Alam Bantarbolang, Jawa Tengah Ulfah Nuraini; Imam Widhiono; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 2 No 2 (2020): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2020.2.2.1756

Kupu-kupu adalah serangga yang termasuk dalam Ordo Lepidoptera, artinya serangga yang hampir seluruh permukaan tubuhnya tertutupi oleh lembaran-lembaran sisik yang memberi corak dan warna sayap kupu-kupu. Keanekaragaman dan Kelimpahan kupu-kupu di pengaruhi oleh faktor lingkungan seperti intensitas cahaya matahari, temperatur, dan kelembapan. Penelitian ini bertujuan untuk mengetahui keanekaragaman dan kelimpahan kupu-kupu di Cagar Alam Bantarbolang, Jawa Tengah. Data yang di dapat di analisis menggunakan indeks keanekaragaman indeks keanekaragaman Shannon-Wiener, indeks kemerataan Shannon-Evenners, dan Indeks dominansi. Hasil penelitian yang telah dilakukan menunjukkan bahwa ditemukan 359 individu dalam 6 familia. Keanekaragaman tertinggi yaitu pada jarak 0 m (H’ = 2.760; E: 0,752). Kelimpahan tertinggi pada jarak 0 m dengan jumlah individu sebanyak 192. Keanekaragaman tertinggi pada jarak 0 m di tepi hutan, sedangkan keragaman terrendah pada jarak 150 m di dalam hutan. Keanekaragaman kupu-kupu didominasi oleh H. glaucippe dari famili Piridae sebanyak 64 individu. Faktor lingkungan yang paling mempengaruhi keanekaragaman dan kelimpahan kupu-kupu adalah intensitas cahaya matahari. Kata kunci: Kupu-kupu, Keanekaragaman, Kelimpahan, Cagar Alam Bantarbolang

Prevalensi dan Kelimpahan Ektoparasit pada Ikan Belanak (Mugil Cephalus) Hasil Tangkapan di Muara Sungai Serayu Adipala Cilacap Ade Rizki Pajrulloh; Rokhmani Rokhmani; Edy Riwidiharso
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 4 No 3 (2022): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2022.4.3.4733

Belanak (Mugil cephalus) is a fish that can adapt to a wide salinity 17-30% and its distribution in tropical to subtropical areas, so that mullet can be found in rivers to river mouths, one of which is found in the Serayu River Estuary, Adipala Cilacap. Fish diseases can be caused by parasites, one of which is ectoparasites. Ectoparasites are organisms that benefit from infesting the body surface of fish. This study aims to determine the prevalence of mullet infested with ectoparasites and to determine the abundance of ectoparasites in mullet (Mugil cephalus) in the Serayu Adipala River, Cilacap. The research method used is survey method with purposive random sampling technique. The variables observed were the prevalence and abundance of ectoparasites. The parameters observed were the type and number of ectoparasites found in mullet. The results showed that the prevalence of ectoparasites in mullet, which was 31Â±13.216%, was included in the general infestation category. The types of ectoparasites found were Dactylogyrus sp. and Apiosoma sp. The abundance of ectoparasites found in mullet is Dactylogyrus sp. of 0.25Â±0.14 ind/head and Apiosoma sp. of 0.36Â± 0.14 ind/head. Both results included in the category of very rare infestations and the highest ectoparasites found in the organs of the mullet fish were found in the gills, namely Dactylogyrus sp. as many as 25 individuals.

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