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All Journal AL KAUNIYAH BIOLINK (Jurnal Biologi Lingkungan, Industri, Kesehatan) Jurnal Biologi Tropis Unri Conference Series: Community Engagement
Wahyu Lestari
Jurusan Biologi, FMIPA Universitas Riau Pekanbaru
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Immunology & microbiology Materials Science & Nanotechnology

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THE DNA SEQUENCE ENCODING GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPC) ENZYME ON TUNTUN ANGIN PLANT (ELAEOCARPUS FLORIBUNDUS BI) Dewi Indriyani Roslim; Hestia Hairima; Herman Herman; Wahyu Lestari
BIOLINK (Jurnal Biologi Lingkungan Industri Kesehatan) Vol 7, No 1 (2020): August 2020
Publisher : Universitas Medan Area

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (498.851 KB) | DOI: 10.31289/biolink.v7i1.3310

Abstract

Glyceraldehyde 3-phosphate dehydrogenase (GapC) is an enzyme involved in glycolysis. The expression of this gene tends to abundant in eukaryotic cells, so this gene is frequently used as an internal control in gene expression analysis. This research aims to isolate the DNA sequence of the GapC gene from tuntun angin (Elaeocarpus floribundus BI). Methods included a collection of the leaves from Kajuik Lake, Riau Province then the DNA extraction, electrophoresis, amplification of partial DNA sequence of GapC gene, cloning and sequencing. The DNA sequence was analyzed using the BLASTn program and MEGA6 software. The GapC sequence obtained in this study was 933 bp in size, consisting of four introns and five exons, and encoding 137 deduced amino acids. The BLASTn analysis showed that the sequence has 89.84%-90.16% similarity to other species of Cunoniaceae family, such as species from the genus of Spiraeanthemum and Codia. The parsial sequence of E. floribundus GapC gene was more resemble the one of Spiraeanthemum than Codia genus. The GapC sequence obtained in this study was the first reported from the Elaeocarpaceae family. This sequence has the opportunity to serve as an internal control after validation.
Optimasi Konsentrasi Ekstrak Alang-Alang (Imperata cylindrica L.) untuk Memacu Pertumbuhan dan Produksi Jagung Manis (Zea mays Saccharata Sturt) Mayta Novaliza Isda; Wahyu Lestari; Diana Agriani
Al-Kauniyah: Jurnal Biologi Vol 6, No 1 (2013): Al-Kauniyah Jurnal Biologi
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/al-kauniyah.v6i1.2829

Abstract

Sweet corn (Zea mays saccharata Struts) a type of corn of high economic value, not only used for consumption also used to make syrup. Imperata is one of the weeds that interfere with crop cultivation, but efforts continue to be made use of weeds as an addition to soil organic matter. The study was conducted to determine the concentration of the extract Imperata (Imperata cylindrica L.) appropriate in the sweet corn growth and determine the concentration of extract proper Imperata in the growth and production. The research method is a Completely Randomized Design (CRD) with 9 treatments and 3 replicates. The results showed that treatment of Imperata extracts with varying concentrations significantly influenced the growth of plant height and leaf number. But not significantly to the growth of the leaf length, leaf width, stem diameter, number of rows per cob, number of seeds per row and plant dry weight. At a concentration of 200 g / 250 ml was able to reduce the vegetative and generative growth of sweet corn.
Pengolahan Sagu menjadi mie prebiotik sebagai makanan fungsional dan upaya peningkatan ketahanan pangan di Desa Alai Selatan, Kecamatan Tebing Tinggi Barat, Kepulauan Meranti Atria Martina; Wahyu Lestari; Tetty Marta Linda; Saberina Hasibuan; Imelda Wardani
Unri Conference Series: Community Engagement Vol 2 (2020): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.2.112-116

Abstract

Noodles are a processed food product favored by most Indonesians, generally made from wheat which must be imported. Kepulauan Meranti Regency is the largest producer of sago in Indonesia. One of the products made from sago that is widely produced in Meranti is sa.go noodle. Making noodles with local raw materials such as sago and sweet potato is an effort to improve food security. Traditional sago noodle has high carbohydrate and fiber content but still has disadvantages such as not having inulin content as a prebiotic, less antioxidant and a less attractive color, while purple sweet potato contains inulin as prebiotic components, high antioxidants, vitamins and minerals.The utilization of prebiotics insago noodle has multiple advantages since they improved probiotic growth in the body, sensory features and provide a more well-balanced nutritional composition as functional food. This community service program aims to training on the process of making prebiotic sago noodles to the community in Alai Selatan Village.This activities are carried out by course and practice methods.The results showed that the participants were interested in making prebiotic sago noodles because they had a more attractive features, more nutritious and had a delicious taste. Prebiotic sago noodles can be used as an effort to biodiversify sago noodle products to support food security and improve the community's economy.
Kebun Organik Di Masa Adaptasi Kebiasaan Baru Pandemi Covid-19 Dengan Memanfaatkan Sampah Rumah Tangga Rodesia Mustika Roza; Atria Martina; Hari Kapli; Bernadeta Leni Fibriarti; Wahyu Lestari
Unri Conference Series: Community Engagement Vol 3 (2021): Seminar Nasional Pemberdayaan Masyarakat
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/unricsce.3.658-664

Abstract

The World Health Organization has designated Covid-19 as a pandemic that causes activities outside the home to be restricted. In order to maintain health during the pandemic, many people are starting to choose foodstuffs produced from organic farming. The purpose of this activity is to provide information and motivation to society for reuse household waste with appropriate technology to produce compost. This service activity was carried out in Air Dingin Village, Bukit Raya District, Pekanbaru. The initial stage conveys material about the importance of maintaining health during the pandemic by consuming organic vegetables and fruits grown in their respective yards. Furthermore, the practice of making compost using the direct-use compost method and composting in a composter pot. Composting that reuses household waste has succeeded in opening up insight into the importance of the community's role in personal and environmental health and the public's understanding that a healthy life doesn't have to be expensive. In this activity, the participants showed their enthusiasm and stated that they would practice and share their knowledge with others in making compost to overcome the problem of household waste. This activity is also beneficial to keep the environment clean, healthy and beautiful.
Germination Capacity of Helianthus annuus Less Seeds on Soil Media Contaminated by Waste Wahyu Lestari; Sujarwati; Atria Martina; Imelda Wardani; Daryono
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4821

Abstract

Used oil waste is often dumped on the ground around the workshop, causing soil pollution which can reduce the function of the soil. The most difficult contaminants in used oil waste to decompose are hydrocarbon compounds, which are highly toxic, polycyclic aromatic hydrocarbons (PAHs). The concentration of oil hydrocarbon contaminants in a sample is determined by measuring the total petroleum hydrocarbon (TPH) concentration. This study was conducted to determine the germination ability of Helianthus anuus, Less seeds on soil media contaminated with waste oil at different concentrations of Total Petroleum Hydrocarbon (TPH). The results showed that the concentration of TPH in the media could inhibit the speed of seed germination. Treatment with low concentration of TPH 2.7% in the media increased % germination, shoot and root length and sprout biomass compared to the medium/intermediate (8.00%) and high (12.26%) TPH treatments and controls. However, there was no significant difference between all treatments regarding germination time and shoot length, although in the low TPH treatment (2.70%) there was a tendency for germination time to be faster than the other treatments, as well as to shoot length which was longer than the other treatments. The higher TPH concentration in the media can inhibit germination time, germination percentage, shoot and root length and biomass. Morphological observations of the growth of sprouts showed an inhibition of root hair formation along with the high TPH content in the media, but all treatments did not affect the growth of the shoots.
Co-Authors Atria Martina Bernadeta Leni Fibriarti Daryono Dewi Indriyani Roslim Diana Agriani Hari Kapli Herman Herman Hestia Hairima Imelda Wardani Mayta Novaliza Isda Rodesia Mustika Roza Saberina Hasibuan Sujarwati Tetty Marta Linda