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All Journal IPTEK The Journal for Technology and Science Indonesian Journal of Biotechnology Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) Journal of Tropical Biodiversity and Biotechnology Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)

Rarastoeti Pratiwi

Universitas Gadjah Mada

Author-ID : 3185657

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Articles

Black Rice Bran Extracts and Fractions Containing Cyanidin 3-glucoside and Peonidin 3-glucoside Induce Apoptosis in Human Cervical Cancer Cells Rarastoeti Pratiwi; Woro Anindito Sri Tunjung; R. Rumiyati; Alfi Rizqi Amalia
Indonesian Journal of Biotechnology Vol 20, No 1 (2015)
Publisher : Universitas Gadjah Mada

Anthocyanin of pigmented rice inhibits the growth of cancer cells. The cytotoxicity and apoptosisinducing properties of local black rice (cv Cempo Ireng) extracts and fractions, which contain anthocyaninincluding cyanidin 3-glucoside and peonidin 3-glucoside, on human cervical cancer cell line (HeLa cells) hasbeen evaluated. The pigmented rice bran was extracted and fractionated using methanol-HCl. The MTT testwas performed on HeLa cell cultures to observe the IC50 value. Preparative TLC was performed to obtain thefractions of black rice bran. Cyanidin 3-glucoside and peonidin 3-glucoside were identified in the pigmentedrice bran extract and fractions using UHPLC. Flowcytometry analysis was performed to measure the percentageof apoptotic cells. Our results suggest that the fractions are more toxic than the methanolic crude extract withIC50 values of 85.95 ± 5.56 μg/mL (the lowest one) and 408.13 ± 51.9 μg/mL, respectively. The concentration ofcyanidin 3-glucoside and peonidin 3-glucoside in the methanolic extract were 1.89 and 0.84 μg/mg, respectively.The apoptosis induction by fractions F2 and F4 (52 and 55%) were significantly higher compared to fractionF3 and F5 (30 and 33%) and doxorubicin (21%). Cyanidin 3-glucoside was detected in F4 (0.14 μg/ml) whilepeonidin 3-glucoside in F2 (0.012 μg/ml), however both were not detected in F3 and F5.

Limited evidence for white spot syndrome virus susceptibility associated with expression of PmVRP15 in local population of giant tiger shrimp (Penaeus monodon) Aushia Tanzih Al Haq; M. Murwantoko; T. Trijoko; Nastiti Wijayanti; Ch. Retna Handayani; Rarastoeti Pratiwi
Indonesian Journal of Biotechnology Vol 20, No 2 (2015)
Publisher : Universitas Gadjah Mada

White spot syndrome virus (WSSV) is a devastating viral disease in shrimp aquaculture. Infection ofWSSV in penaeid shrimps affects immune defense and changes gene expression. PmVRP15 has been reported as a part of the WSSV propagation pathway that is highly up-regulated in hemocytes at the acute phase of WSSV infection. This study analyzed the expression of PmVRP15 in local populations of giant tiger shrimp (Penaeus monodon) to be associated with susceptibility to WSSV. Tested populations consisted of an inbreeding population (G8) and outbreeding population (G8iA) from Jepara, Indonesia. Susceptibility was determined by cumulative mortality, median lethal time (LT50), and severity of infection at time of death. Though all populations were susceptible to WSSV, the frst mortality in G8 occurred at 18 hours post-infection (hpi) with mild infection, while frst mortality of G8iA occurred at 30 hpi with severe infection. The LT50 of G8 was signifcantly lower than that of G8iA, indicating that G8iA was less susceptible to WSSV than G8. Relative PmVRP15 transcripts of G8iA were insignifcantly down-regulated, whereas relative PmVRP15 transcripts of G8were insignifcantly upregulated. Although it’s still not conclusive, the results of this study suggest that PmVRP15 has weak potentialas a WSSV susceptibility marker in G8 and G8iA broodstock selection.

Anthocyanin, nutrient contents, and antioxidant activity of black rice bran of Oryza sativa L. ‘Cempo Ireng’ from Sleman, Yogyakarta, Indonesia Pratiwi Apridamayanti; Rarastoeti Pratiwi; Yekti Asih Purwestri; Woro Anindito Sri Tunjung; Rumiyati Rumiyati
Indonesian Journal of Biotechnology Vol 22, No 1 (2017)
Publisher : Universitas Gadjah Mada

The chemical contents and health benefits of black rice bran of some rice cultivars have been investigated. However, there has been little research on the ‘Cempo Ireng’ cultivar from Sleman, Yogyakarta. The aim of this present study was to determine the anthocyanin, antioxidant activity, and macro- and micronutrients contents of black rice bran from this local cultivar. The anthocyanin in the black rice bran was extracted using the maceration method with methanol as a solvent. The extract obtained was separated through a preparative thin layer chromatography (TLC) of silica GF254 and a mobile phase composed of n-butanol, acetic acid, and water. Two fractions were collected and analyzed for the anthocyanin content. The preparative TLC spots were separated for further detection and measurement of cyanidin 3-O-glucoside using HPLC followed by LC-MS. The antioxidant activity of the fractions were measured using the DPPH free radical scavenging method. The results showed that the anthocyanin in fraction 1 was identified as cyanidin 3-O-glucoside (66.1 ± 10.6 µg/g). The IC50 of fractions 1 and 2 were 200.96 and 218.36 µg/mL, respectively. Analysis of the macro- and micronutrients revealed that the black rice bran of ‘Cempo Ireng’ had nutrient contents comparable with other rice cultivars. Therefore, this local black rice bran can be used as a source of antioxidants and macro-- and micronutrients.

The effect of ethanolic extract of black and white rice bran (Oryza sativa L.) on cancer cells Rizal Maarif Rukmana; Nyoman Puniawati Soesilo; Rumiyati Rumiyati; Rarastoeti Pratiwi
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Indonesia has a wide range of rice cultivars and pigments. This rice can be used as a source of phytochemical compounds for cancer prevention. This research aims to analyze the cytotoxic activities of the ethanolic extract of black rice bran of 4 local cultivars i.e. ‘Cempo Ireng’, ‘Woja Laka’, ‘Toraja’ and ‘IR64’ (white rice) on cancer cells and to determine the compounds groups of those extracts. First step, rice bran was extracted with ethanol. This extract was applied to Raji (a human Burkitt Lymphoma cancer), HepG2 (a human liver cancer), and Vero (a nonhuman cell line) cells in order to measure the cytotoxic activities by using MTT assay. To determine descriptively the compounds groups of phenolics, flavonoids, terpenoids, steroids, and alkaloids the thin layer chromatography method was performed. The IC50 value was analyzed quantitatively by using probit analysis. Results showed that the IC50 values of ethanolic extract of rice bran ‘Woja Laka’, ‘Toraja’, ‘Cempo Ireng’ and ‘IR 64’ on HepG2 cells were 857.23±99.19; 1,896.55±83,8; 1,494.47±87.81 and 727.89±145,97 µg/ml respectively. The IC50 on Raji cells were 816.61±85.31; 1,079.93±28.31; 1,627.82; ±119.82, and 769.33±61.43 µg/ml respectively. The IC50 on Vero cells were 1,295.2±37; 1,232.07±165.51; 1,874.14±169.56, and 724.4±122.79 µg/ml respectively. The ethanolic extracts of rice bran from four cultivars contain phenolics, flavonoids, terpenoids, and steroids. However, alkaloids could not be detected. The variety of rice cultivars indicates the variation of cytotoxic activities on cancer cells. The ethanolic extracts of rice bran from those four rice cultivars contain similar kinds of organic compounds groups but vary in the Rf values.

Cytotoxic activity and apoptosis induction of avocado Persea americana Mill. seed extract on MCF-7 cancer cell line Yuli Widiyastuti; Rarastoeti Pratiwi; Sugeng Riyanto; Subagus Wahyuono
Indonesian Journal of Biotechnology Vol 23, No 2 (2018)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.32141

Avocado Persea Americana Mill. is a commercially important crop and studies have shown that the pulp may have benefits to cardiovascular health, dermatological health and possibly anti-cancer activity. Avocado seeds have several medicinal properties such as anti-hyperglycemic, antimicrobial, antioxidant and anti-inflammation. This study aim to evaluate the effect of avocado seed extract on viability and apoptosis of breast cancer cell line MCF-7. The anticancer effect was evaluated by cytotoxic test using MTT assay and the effect on apoptosis and cell cycle was examined by flow cytometry method. The cytotoxic test showed that chloroform extract had strong cytotoxic activity against MCF-7 cell lines with IC50 value of 94.87 µg/mL. Furthermore, the chloroform extract was partitioned with methanol and yield of soluble methanol fraction (FLM) and non soluble methanol fraction (FTLM). The cytotoxic activity of the methanol soluble fraction (FLM) and non soluble methanol fraction (FTLM) against MCF-7 cell lines was increased with IC50 of 34.52 and 66.03 µg/mL, respectively. Flow cytometry analysis using annexin-V and propidium iodide staining revealed that methanol soluble fraction could induce apoptosis and modulating the cell cycle arrest in MCF-7 cell. This research indicated that avocado seed has a potency to induce apoptosis and as anti-proliferative to MCF-7 cells lines.

Pemanfaatan Limbah Buah Salak Pondoh Sebagai Substrat Nata De Salacca Melalui Aplikasi Bioteknologi di Dusun Tegal Domban, Sleman, Yogyakarta Rarastoeti Pratiwi; Fajar Budi Lestari; Donny Widianto
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 1, No 1 (2015): September
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Dusun Tegal Domban is one of the highest Salak Pondoh produce in Sleman District. However there is a problemfaced by salak farmers due to the overproduction and overripe which mightcause waste. Implementation of Biotechnology should be one of the alternativesolutions to overcome this problem. The aims of the program were to utilize the salak fruits wasteto produce Natade Salaccausing biological agents, such as Acetobacter xylinum, and to implement the education for sustainability development which subjected toa women organization PKK Dusun Tegal Domban who accompanyed by the team from Biotechnology Master Program UGM in order to learn how to produce Nata de Salacca. This program was initiated with the laboratory experiments to find the fine composotion of nata substrates to get the optimal nata product. The following action was to acompany the women who aplicate the nata production process. Results from the laboratory experiment showed that the best composition of substrat and water ratiowas 1:4, and the nata thickness was 0.62 cm, while the nata weight was 542.22 g. Those parameters were used for nata standard indicators.Results from the activities of nata production by women groups PKK Dusun Tegal Domban were showed the similar results with the nata standard for thickness, however nata weight was slightly lower than the nata standard.

Kandungan Fenolik, Flavonoid dan Aktivitas Antioksidan Ekstrak Daun Paku Laut (Acrostichum aureum L.) Fertil dan Steril di Kawasan Mangrove Kulon Progo, Yogyakarta Naovi Nur Fadia Hanin; Rarastoeti Pratiwi
Journal of Tropical Biodiversity and Biotechnology Vol 2, No 2 (2017): December
Publisher : Universitas Gadjah Mada

Sea Fern (Acrostichum aureum L.), which typically grows in mangrove ecosystem, has two distinguished leaves which are fertile leaves (rich in spores) and sterile leaves (no spores). Those leaves are known rich in phenolic compounds. Flavonoids, moreover, which belong to class of phenolic compound, have antioxidant activity. This study aims were to analyze the phenolic content, flavonoid content, and antioxidant activity of fertile leaves, sterile leaves, and spores of sea fern which grow in mangrove ecosystem that is located in Kulon Progo, Yogyakarta. Leaf and spore samples were extracted using ethanol solvent. Then, total content of phenol and flavonoid were measured by spectrophotometry, while the antioxidant activity was measured by DPPH method. The results showed that the phenolic content of fertilized leaves and sterile leaves did not show a significant difference. In other hand, the spores sample showed highest both of flavonoids content (2.32 mg/mL) and the antioxidant activity with the IC50 value was 23.25 ppm. Thus, the ethanolic extracts of spores of sea fern sterile leaves showed the highest flavonoid content and could be categorized as a very strong antioxidant.

The Anticancer Activity of Marine Sponge Cinachyrella sp. (Family Tetillidae) Awik Puji Dyah Nurhayati; Rarastoeti Pratiwi; Subagus Wahyuono; Istriyati Istriyati; Nichole J de Voogt
IPTEK The Journal for Technology and Science Vol 25, No 3 (2014)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v25i3.530

Cinachyrella sp. marine sponge produce many kinds of secondary metabolites. The purpose of this study was to examine the cytotoxic and anti-proliferative activity of marine sponge Cinachyrella sp. The sponge was extracted with 96 % ethanol. Ethanol extract cytotoxicity assay were performed with MTT method (Microculture Tetrazolium) against to cell lines of HeLa, T47D, WiDr and Vero. The results show that of the ethanol extract were only toxic to cell lines of HeLa IC50 897.809 μg/mL but did not toxic to cell lines of T47D, WiDr and normal cell lines of Vero. Fractionation of the ethanol extract was conducted by a Vacuum Column Chromatography (VCC) and have 4 fraction that were F1, F2, F3 and F4. Cytotoxicity and cell proliferation inhibitory were tested of fraction F1, F2, F3 and F4 against cell lines of T47D. The values IC50 of F1; F2; F3 and F4 against cell lines of T47D were 82.744; 163.679; 66.522 and 333.026 μg/mL and fraction F3 concentration 31.5 µg/mL inhibits cell proliferation cell lines of T47D at 48 hours of incubation

Pembelajaran Biologi Molekular dan Bioteknologi dalam Konsep Merdeka Belajar Rarastoeti Pratiwi
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2022: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar

Pendidikan merupakan kebutuhan dasar umat manusia. Setiap insan yang dilahirkan memiliki hak yang sama untuk mendapatkan pendidikan yang layak. Proses pendidikan mencakup 4 pilar pembelajaran, yakni learning to know, learning to do, learning to be, dan learning to live together. Keempat pilar tersebut memiliki aspek yang sangat luas untuk dapat diterapkan. Salah satunya, dikaitkan dalam pembelajaran IPTEK (Ilmu Pengetahuan dan Teknologi) bidang Biologi, khususnya mata pelajaran Biologi Molekuler dan Bioteknologi. Selain itu, pembelajaran dapat dikaitkan dengan tingkatan (leveling) peserta didik, dari tingkat Pra Sekolah (Pendidikan Anak Usia Dini dan Taman Kanak-kanak), Sekolah Dasar, Sekolah Menengah Pertama atau sederajad, Sekolah Menengah atas atau sederajad, hingga Perguruan Tinggi. Pendidikan dan pembelajaran perlu menyesuaikan perkembangan jaman, dengan tantangan masing-masing jaman yang berbeda, khususnya terkait IPTEK. Dalam paparan topik ini, akan diulas lebih jauh tentang pembelajaran Biologi Molekuler dan Bioteknologi dalam konsep Merdeka Belajar, yang dikaitkan dengan tingkatan peserta didik. Dalam rangka mengikuti, mengisi dan mengantisipasi perkembangan jaman terkini hingga berikutnya, Kementerian Pendidikan dan Kebudayaan Republik Indonesia telah menerapkan program Merdeka Belajar. Beberapa konsep tentang program Merdeka Belajar, antara lain arah kebijakan yang meliputi pengembangan literasi, numerasi dan karakter siswa. Selain itu, pelaksanaan proses pendidikan menjadi lebih otonom dengan kultur pembelajaran yang lebih inovatif. Diharapkan dengan konsep Merdeka Belajar, guru dan peserta didik lebih termotivasi dalam mengikuti proses pembelajaran, hingga tercapai tujuan pendidikan maupun pembelajaran secara optimum, serta dilaksanakan secara lebih membahagiakan, termasuk didalamnya pembelajaran untuk mata pelajaran Biologi Molekuler dan Bioteknolgi.

Co-Authors Alfi Rizqi Amalia Aushia Tanzih Al Haq Awik Puji Dyah Nurhayati Ch. Retna Handayani Donny Widianto Fajar Budi Lestari Istriyati Istriyati Murwantoko . Naovi Nur Fadia Hanin Nastiti Wijayanti Nichole J de Voogt Nyoman Puniawati Soesilo Pratiwi Apridamayanti R. Rumiyati Rizal Maarif Rukmana Rumiyati Rumiyati Rumiyati Rumiyati Subagus Wahyuono Subagus Wahyuono Sugeng Riyanto T. Trijoko Woro Anindito Sri Tunjung Yekti Asih Purwestri Yuli Widiyastuti

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