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Leni Maylina
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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KIVP-3 Pengamatan Performa Pada Sapi FH Jantan yang Diberi Suplementasi Mineral Zinc Sus Derthi Widhyai; Dondin Sajuthi; Setyo Widodo; Anita Esfandiari; Retno Wulansari; Agus Wijaya; Chusnul Choliq; Agus Lelana; Leni Maylina; Arief Purwo Mihardi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Permintaan dunia terhadap protein hewani sangat besar dan terus meningkat setiap tahunnya. Kebutuhan yang meningkat tidak dibarengi dengan kemampuan penyediaan protein hewani yang cukup sehingga sebagian besar dipenuhi melalui import. Pemenuhan protein dalam negeri diharapkan mampu meningkatkan kualitas maupun kuantitas daging melalui perbaikan mutu nutrisi sehingga mampu bersaing dengan pihak luar. Salah satu sumber protein hewani selain dari ternak potong, adalah memanfaatkan sapi jantan dari sapi perah FH. Oleh karena itu ternak jantan dapat digunakan sebagai alternatif sumber protein hewani dalam upaya untuk penyediaan pangan asal ternak.  Pembangunan peternakan diarahkan agar produk ternak dalam negeri mampu bersaing dengan produk ternak impor dalam rangka memantapkan ketahanan pangan nasional. Mineral Zn dilaporkan mampu memperbaiki skor marbling karkas [1].  Hal ini penting untuk memperbaiki kualitas karkas daging sebagai sumber protein hewani. Belum banyak informasi tentang efek suplementasi Zn terhadap pertambahan bobot badan terutama pada sapi FH jantan. Oleh karena itu pengamatan tentang suplementasi Zn terhadap performa (bobot badan) pada sapi FH jantan perlu dilakukan.
KIVFA-2 Efek Imunomodulator terhadap Profil Leukosit Induk Sapi Friesian Holstein yang Diberi Antigen AI H5N1 Inaktif Sri Murtini; Anita Esfandiari; Sus Derthi Widhyarti; Retno Wulansari; Leni Maylina; Arif Purwo Mihardi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Immunomodulator adalah zat yang memiliki kemampuan untuk meningkatkan  atau menekan respon imun. Cox (1988) melaporkan bahwa pengaruhnya  selain terhadap  respon imun, imunomodulator juga dapat memodulasi haematopoiesis, termasuk peningkatan jumlah RBC dan WBC (leukosit), peningkatan PCV dan aktivasi makrofag.  Sapi friesian holstein (FH) merupakan sapi perah yang dapat digunakan sebagai hewan donor penghasil immunoglobulin G anti AI H5N1 melalui produk kolostrumnya (Esfandiari et al, 2007).  Guna meningkatkan titer immunoglobulin pada hewan donor hiperimun sera umumnya hewan diberi imunomdulator.  Berbagai jenis bahan seperti glucan, lectin, dan berbagai jenis polisakarida dari tanaman maupun hewan (Alamgir dan Uddin 2010) serta polipeptide ribonukleotida dapat digunakan sebagai imunomodulator.(Hess dan Greenberg 2012)  Penggunaan  polipeptida ribonukleotida sebagai imunomodulator ada sapi saat ini belum banyak diteliti.  Pemberian imunomodulator memberikan dampak perubahan gambaran leukosit secara langsung maupun tidak langsung.  Dampak pemberian imunomodulator jenis polipeptide ribonukleotida pada sapi FH bunting trimester terakhir untuk tujuan produksi hiperimunsera melalui produksi kolostrum belum pernah dilaporkan.  Penelitian ini bertujuan untuk mengetahui efek pemberian imunomodulator peptide ribonukleotida pada sapi FH yang disuntik antigen AI H5N1 inaktif.
PF-4 Immune Response of Dry Holstein Vaccinated by Killed Avian Influenza H5N1 Vaccine Anita Esfandiari; Sri Murtini; Sus Derthi Widhyari; Retno Wulansari; Bayu Febram; Leni Maylina; Arief Purwo Mihardi
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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The establishment and spread of highly pathogenic avian influenza (HPAI) viruses of the H5N1 subtype in birds and coincident infections in humans since 2003 have raised concerns that we may be facing an influenza pandemic caused by an H5N1 influenza virus [4]. Globally, from January 2003 to 2 March 2017, there were 860 cases of human infection with avian influenza A(H5N1) virus reported from 16 countries worldwide. Of these 860 cases, 454 were fatal (CFR of 53%). The last case was reported from Indonesia on 26 September 2017 [7]. Although the human cases of H5N1 in Indonesia has decreased significantly since 2010, according to WHO until 2017 there have been reported 200 cases H5N1 in  human with 168 cases of deaths. The last case was reported from Indonesia on 26 September 2017. This is indicate Indonesia as the highest fatal case of H5N1 globally. With continued incidence of avian influenza due to existing AI H5N1 viruses in poultry, the avian influenza H5N1 has been believed remain to threaten Indonesia [6].Passive immunization using specific antibody against AI H5N1 from bovine colostrum is one of an alternative to control H5N1 virus infection due to lack of H5N1 vaccine production for human. Bovine colostrums consider an ideal alternative antibody source, as the antibody in the bovine’s blood is transported to mammary gland easily and accumulates in the colostrum in large quantities. As a “biological factory”  and the source of natural antibody, bovine colostrum could be designed to produce the specific antibody against certain disease for animal and human by immunizing the dry cow (with the antigen of interest).Vaccine is an antigenic material used to produce active immunity against diseases. Vaccination is the administration of vaccine to the individual to generate immunity against a disease [2]. According to [1], the exposure of a dry cow against antigen (vaccine) will produce specific antibody in their blood circulation. Therefore, evaluation on the status of antibody against AI H5N1 following the vaccination of dry Holstein cows with killed AI H5N1 vaccine is needed. 
Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini; Bayu Febram; Retno Wulansari; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to detect antibody (IgG) against enterotoxigenic Escherichia coli (ETEC)K99 in the blood of cows vaccinated by Escherichia coli polyvalent vaccine. Eight dry cows were injectedsubcutaneously by polyvalent Escherichia coli twice prior to parturition. Before vaccinated, the cows were givenimmunomodulator orally for 3 days. Blood samples were drawn from coccigeal vein prior to the 1st vaccination,two week following the 1st vaccination and at 1, 2, and 4 weeks after the 2nd vaccination. Blood samples wereanalyzed for IgG and ETEC K99 using indirect Enzyme Linked Immunosorbent Assay (ELISA) assay. Results of theexperiment indicated that absorbance values of all vaccinated cows before the first vaccination until third weekfollowing the 2nd vaccination were below cut off values. The absorbance values then increased and were above cutoff values at fourth week following the 2nd vaccination. In conclusion, antibody against ETEC K99, were detected inthe blood of cows, fourth week following the 2nd vaccination.
Efek Penambahan Mineral Zn Terhadap Gambaran Hematologi pada Anak Sapi Frisian Holstein Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 3 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to determine the effects of zinc supplementation on health status in dairy calves. Nine Frisian Holstein (FH) at 6-10 months of age were used in this experiment and divided into three groups. First group (no added Zn) for control, the second group was added 60 ppm Zn, and the third group was added 120 ppm Zn. Zn was administered daily for three months. Blood samples were collected from the jugular vein and anticoagulated with EDTA. Whole blood were used for measuring erythrocytes, hemoglobin concentration, hematocrit value, total leukocyte count, and leukocyte cell types. The results showed that no difference among groups for hematological parameters and the value of hematology were in the range values references. In conclusion, 60 and 120 ppm Zn supplementation in the feed is relatively safe for health.
KIVFA-8 Studi Kasus: Profil Mineral Makro Pada Sapi Perah Yang Mengalami Retensi Plasenta di Kunak Kabupaten Bogor Retno Wulansari; Anita Esfandiari; Sus Derthi Widhyarti; Chusnul Choliq; Arief Purwo Mihardi; Leni Maylina; . Suryono
Hemera Zoa Proceedings of the 20th FAVA & the 15th KIVNAS PDHI 2018
Publisher : Hemera Zoa

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Abstract

Susu merupakan salah satu bahan pangan yang sangat penting bagi masyarakat untuk memenuhi kebutuhan gizinya. Peningkatan kesadaran masyarakat akan pentingnya susu sebagai salah satu sumber gizi yang penting berefek terhadap kebutuhan susu nasional terus meningkat pula. Namun kebutuhan yang tinggi ini belum diimbangi dengan produksi susu nasional yang baru mencapai 3.29% per tahun, sehingga kekurangan akan kebutuhan susu ini masih harus diimport dari negara lain. Rata-rata produksi susu di Indonesia dari setiap sapi masih relatif rendah, sekitar 10-12 liter/ekor/hari (Deny 2014). Keterbatasan produksi susu dari dalam negeri ini disebabkan oleh masih belum maksimalnya produksi susu dari setiap sapi perah yang dimiliki oleh peternak di Indonesia.Manajemen pemeliharaan yang baik dalam usaha peternakan sapi perah sangat diperlukan untuk dapat meningkatkan produksi susu, salah satunya antara lain manajemen pakan. Pakan harus memenuhi unsur-unsur penting diantaranya mineral makro dan mikro dalam jumlah yang secukupnya.Masalah kesehatan yang sering ditemui pada sapi perah awal laktasi umumnya berupa gangguan metabolik, seperti milk fever dan ketosis (Divers & Peek 2008). Hipokalsemia adalah kelainan metabolik dimana mekanisme homeostasis gagal untuk mempertahankan konsentrasi Ca darah normal saat awal laktasi (Chamberlain et al. 2013). Kejadian ini sering didahului dengan kondisi hipokalsemia subklinis pada saat bunting dan kering kandang, tetapi tidak teramati oleh peternak (Goff 2008). Retensi Plasenta merupakan salah satu manifestasi dari gangguan metabolik akibat tidak cukupnya konsentrasi mineral pada hewan post partus. Sapi secara normal akan melepaskan plasenta dalam waktu 3 -6 jam post partus. Retensi atau tertahannya plasenta lebih dari 8 – 12 jam pada induk post partus dapat dipertimbangkan sebagai suatu kondisi yang abnormal (Diver & Peek 2008. Salah satu predisposisi adalah tidak adanya program manajemen pakan atau pemberian suplemen mineral yang tidak tepat, sebagaimana disajikan pada studi kasus berikut.
Tinjauan Penambahan Mineral Zn dalam Pakan Terhadap Kualitas Spermatozoa pada Sapi Frisian holstein Jantan Sus Derthi Widhyari; Anita Esfandiari; Agus Wijaya; Retno Wulansari; Setyo Widodo; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 1 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Bulls are expected to be able to produce a good quality and quantity of sperm. The objective of this experiment was to study the effect of Zn supplementation on the sperm quality, in Frisian holstein bulls. Ten bulls, 1618 months of age were used in this experiment. The experimental bulls were divided into two groups, i.e.,group without Zn supplementation (control) and group with 60 ppm of Zn supplementation. Zn supplementations were given everyday for a period of four months. Semen was collected by using artificial vaginaat the end of the experiment. Semen quality was evaluated macroscopically and microscopically. The variables measured were semen volume, semen pH, sperm motility, sperm concentration, sperm viability, and sperm abnormality. The results showed that Zn supplementation significantly increased sperm motility and sperm concentration (P<0.05), whereas there was no significant difference in other parameters.
Co-Authors . Suryono Agus Lelana Agus Wijaya Anita Esfandiari Arif Purwo Mihardi Bayu Febram Chusnul Choliq Dondin Sajuthi Mihardi, Arief Purwo Retno Wulansari Setyo Widodo Sri Murtini Sus Derthi Widhyai Sus Derthi Widhyari Sus Derthi Widhyarti