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DIVERSITY OF FRUIT FLIES (TEPHRITIDAE: BACTROCERA SPP.) IN CAMPUS C OF AIRLANGGA UNIVERSITY, SURABAYA, INDONESIA Eka Kartika Arum; Moch. Affandi; Sucipto Hariyanto
TREUBIA Vol 47, No 2 (2020): Vol. 47, No. 2, December 2020
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/treubia.v47i2.3982

Abstract

This research aims to get information about the species of host plants and fruit flies, composition and structure of community, distribution pattern, and impact of environmental factors to fruit flies in Campus C, Airlangga University. Research was conducted from August to November 2019. A modification of Steiner trap with methyl eugenol 1.5 ml bait was installed in nine sites. Each Steiner trap was placed on a mango tree 1-2 meters above ground level. Trapped fruit fly specimens were collected after one week. Four replications were made, with intervals between two periods of installation. As many as 682 host plants of the fruit flies were found at the study site consisting of 25 species from 15 families. Results showed that 1121 individuals of Bactrocera fruit flies were found, consisting of 5 species, namely B. carambolae, B. dorsalis, B. minuscula, B. occipitalis, and B. musae. The most abundant species was B. carambolae (62.8%), followed by B. dorsalis (27.3%), B. minuscula (8.4%), B. occipitalis (1%), and the lowest was B. musae (0.5%). B. occipitalis has an even distribution pattern, while four other species have aggregated distribution patterns. The diversity index at nine locations ranged from 0.772 (low) to 1.151 (moderate). B. occipitalis has an even distribution pattern, while five other species have aggregated distribution patterns. The diversity index at nine locations ranged from 0.855 (low) to 1.328 (moderate). B. carambolae and B. dorsalis were the dominant species. The presence of fruit flies was influenced by environmental (humidity, temperature, sunlight intensity, wind) and host plant factors.    
DIVERSITY OF THE UNIONID FRESHWATER MUSSELS (BIVALVIA: UNIONIDAE) IN BRANTAS RIVER, EAST JAVA, INDONESIA Moch. Affandi; Leonard Ady Candra; Astra Budi Priatama; Bambang Irawan; Agoes Soegianto
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 18 No 2 (2013): June 2013
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (831.966 KB) | DOI: 10.23869/145

Abstract

The aims of this study are to provide data and information about diversity, dominance, and distribution of the Unionidae mussels species in Brantas River, East Java, Indonesia. Mussel samples were collected from 14 sampling stations along the Brantas River, starting from upstream in Tulungagung to downstream in Surabaya (± 170 km) in April-May 2012. The results showed that five species of mussels, namely: Contradens contradens, Elongaria orientalis, Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis were observed in the Brantas River. Contradens contradens was the “dominant” species, followed by Elongaria orientalis as a “common” species, and Rectidens sumatrensis, Pseudodon vondembuschianus and Pilsbryoconcha exilis as “rare” species. Distribution of each species in the Brantas River tends to be concentrated in downstream of the river, mainly located in station 13. According to the IUCN Red List, Rectidens sumatrensis was categorized in “Data Deficient”, while Pseudodon vondembuschianus and Pilsbryoconcha exilis were categorized in “Least Concern”.
Water quality and fish diversity in the Brantas River, East Java, Indonesia Alfiah Hayati; Nureka Tiantono; Muhamad Fadhil Mirza; Iman Dary Supriyadi Putra; Muhamad Maulana Abdizen; Antien Rekyan Seta; Binti Mar`atus Solikha; Muhamad Hilman Fu'adil; Trisnadi Widyaleksono Catur Putranto; Mochamad Affandi; Rosmanida Rosmanida
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 22 No 2 (2017): June 2017
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (701.153 KB) | DOI: 10.23869/100

Abstract

This research aimed to determine the water quality and fish diversity in Brantas river. Three station for sampling locations were in the upstream (one station on Karangkates reservoir) and downstream (two stations on the Surabaya and Jagir river). Water quality parameters were BOD, COD, DO, pH, temperature, and the levels of heavy metals (Pb, Cr, Cu, and Cd). Fish specimens was identified using fish identification book. The results of this research indicated that there was a difference in the water quality of upstream and downstream of the Brantas river. On the upstream, the source of pollution was mostly from fish catching, agriculture, and industrial activities. While on the downstream of the river it mostly dominated by industrial waste and domestic waste. The water quality in both stations had far exceeded the quality standards that was included in the polluted category. The concluded that Brantas river has been contaminated by waste (heavy metals), exceeded the water quality standard. The diversity of fish in the downstream (Surabaya river) has the highest diversity values, followed Jagir river and in the upstream (Karangkates reservoir) has the lowest value of diversity. Brantas river water was included in the category of moderate diversity.
DIVERSITAS GASTROPODA DI SUNGAI SUKAMADE, TAMAN NASIONAL MERU BETIRI, JAWA TIMUR Putut R. Purnama; Nimas W. Nastiti; Melia E. Agustin; Moch. Affandi
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 16 No 2 (2011): June 2011
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/299

Abstract

Sukamade River is one of the main rivers in Meru Betiri National Park, especially at Sarongan Resort. Macrofauna benthic community in this river are in the protected areas that allegedly still original, and has not got the impact of human activity. Information about the diversity of gastropods class in Sukamade River in limited encourage to do research on the diversity of gastropods in this place. This research was conducted to determine the diversity of gastropods in Sukamade River. Gastropods samples taken at five stations those are in estuary, middle, and part of the river that closer to upstream river using surber-net and hand net. Surber-net used to take samples in watersheds zones and riparian zones using hand net. Samples were obtained, inserted the container that contains the fixative solution that formed by formalin 6% for preservation. Samples that have been preserved, and then sorted, identified and counted the number of individuals of each species, and data analysis. Data analysis, among others, to calculate the diversity index, evenness index, and dominance index in the Laboratory of Ecology Department of Biology, Airlangga University. From the analysis, obtained by the number of species found are 20 species that range from 8–14 species per station, the diversity index ranged from 1.860 to 0.795, evenness index ranged from 0.383 to 0.723 and the dominance index ranged from 0.223 to 0.659. Can be concluded that the diversity of gastropods in Sukamade River is at low-medium category.
Ability Test of IAA (Indole-3-Acetic Acid) Hormone-Producing Endophytic Bacteria from Lamongan Mangrove Fatimah Fatimah; Risky Lailatul Ayu Fadilah; Annida Izzatul Millah; Tri Nurhariyati; Bambang Irawan; Ni’matuzahroh Ni’matuzahroh; Moch Affandi; Afifa Rini Nur Izza Zuhri; Eva Watamtin Widhiya; Syarifah Salsabila; Zakia Asrifah Ramly
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 1 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n1.p42-50

Abstract

Most of the plant-associated bacteria can synthesize active biological components of phytohormones such as auxin. This study aimed to examine the potency of 61 endophytic bacteria isolates from the mangroves at Kutang Beach, Lamongan in producing IAA hormone and to identify types of isolates effecting the concentration of IAA, morphological characteristics of isolates, as well as endophytic bacterial species that have the most potential to produce IAA hormone. Screening of endophytic bacteria isolates was performed using the colorimetric method and the production of IAA was carried out using the spectrophotometric method. IAA production by endophytic bacteria was analyzed descriptively and statistically. One-Way ANOVA was employed to determine the effect of the isolate type on the concentration of IAA. The most potential isolates to produce IAA hormone are identified by 16s rRNA gene marker. The screening results showed that 12 isolates of endophytic bacteria have the potential to produce IAA hormones (2.0-9.3 ppm), coded with LMG 7, 15, 31, 32, 43, 53, 54, 55, 56, 57, 62, and 63. The results of the One-Way ANOVA test suggested that the type of isolates did not affect the concentration of IAA produced by endophytic bacteria. The twelve isolates had different morphological characters and those were Gram-positive bacilli with cell sizes ranging from 1.5 µm - 3 µm. The highest concentration of IAA was produced by LMG 15 (9.3 ppm). LMG 15 was identified as Bacillus cereus strain LMG 15, having 99.33% similarity to Bacillus cereus strain IAM 12605.
Isolation and Potency Test of Endophytic Bacteria as Nitrogen Fixer from Mangrove Plant in Lamongan Fatimah Fatimah; Annida Izzatul Millah; Risky Lailatul Ayu Fadilah; Syarifah Salsabila; Zakia Asrifah Ramly; Tipuk Sugiarti; Tri Nurhariyati; Ni’matuzahroh Ni’matuzahroh; Moch Affandi
Jurnal Riset Biologi dan Aplikasinya Vol. 4 No. 1 (2022)
Publisher : Universitas Negeri Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26740/jrba.v4n1.p26-33

Abstract

Endophytic bacteria are microorganisms that live in plant tissues and some of them contribute to nitrogen fixation for plants. This study aimed to isolate and identify endophytic bacteria from mangroves of Kutang Beach, Lamongan, which potentially as nitrogen fixing bacteria. Bacterial Isolates were used as candidates for biofertilizers. Leaves samples were taken from 10 sampling points. Bacterial isolation was initiated by sterilizing the surface of the leaves sample and grinding it aseptically. Isolation was carried out with a pour plate method on Nutrient Agar medium. Screening for endophytic bacteria's potential as N-fixing agent was carried out by growing the bacterial isolates on a semi-solid Nitrogen Free Bromothymol Blue (NFB) medium. The isolates that produced a positive reaction with a change in the color of the medium to blue were then subjected to macroscopic (shape, color, elevation, and the edge of the colony) and microscopic observations (Gram stain and bacterial cell measurements). The isolates showed the fastest change in the color of the medium were identified by the molecular marker of the 16S rRNA gene. The data obtained were analyzed descriptively. As many as 20 isolates were obtained from the mangroves of Kutang Lamongan Beach, and ten isolates of twenty potentially as nitrogen-fixing bacteria. The ten nitrogen-fixing bacteria isolates had varying macroscopic characteristics. The microscopic characteristics showed that eight isolates had Gram-positive bacilli, and two isolates were Gram-negative with varying bacterial sizes. Based on the 16S rRNA gene sequence, the most potential of nitrogen-fixing bacteria was LMG II-14 isolate and identified as Paenibacillus alvei LMG II-14 with 99.36% similarity to Paenibacillus alvei strain DSM 29 based on the NCBI database. The ten nitrogen fixing isolates that have been obtained can later be used as candidates for biofertilizer composition, especially Paenibacillus alvei LMG II-14.