Article Per Year (5 Year)
p-Index From 2019 - 2024
0.659
P-Index
This Author published in this journals
All Journal Jurnal Fitopatologi Indonesia BERITA BIOLOGI JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Entomologi Indonesia Jurnal Pusat Inovasi Masyarakat (JPIM)
Kusumah, Yayi Munara
Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology Social Sciences

Published : 8 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 8 Documents
Search

 1 
KEEFEKTIFAN BAHAN PELINDUNG ALAMI DALAM MEMPERTAHANKAN INFEKTIVITAS Spodoptera exigua NUCLEOPOLYHEDROVIRUS (SeNPV) Samsudin, Samsudin; Santoso, Teguh; Rauf, Aunu; Kusumah, Yayi Munara
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (388.322 KB) | DOI: 10.14203/beritabiologi.v10i6.1932

Abstract

Spodoptera exigua nucleopolyhedrovirus (SeNPV) is a viral pathogen of onion caterpillar S. exigua with high pathogenicity. One of the major constraints to the use of SeNPV for biocontrol of onion caterpillar is its sensitivity to ultraviolet (UV) degradation. The purposes of this research were to determine the effect of sunlight exposure on the virulence of SeNPV and to find out the effective natural UV protectant to maintain the SeNPV virulence. The results showed that the sunlight radiation affects the SeNPV infectivity. Addition of 1% of coconut shell charcoal, lampblack, husk charcoal, yam flour, molasses, yam filtrate, turmeric filtrate and green tea filtrate to the SeNPV suspension were found to be effective as UV protectant. Coconut shell charcoal, lampblack and husk charcoal are activated carbon that can absorb UV light. Yam filtrate is a natural ingredient that contains saponins and is able to protect SeNPV particles as reflectance. While molasses, turmeric filtrate and green tea filtrate containing flavanoid serve as a protective virus particles and UV absorber.
KARAKTERISASI MOLEKULER NUCLEAOPOLYHEDROVIRUS (NPV) HYPOSIDRA TALACA WLK. DI PERKEBUNAN TEH GUNUNG MAS BOGOR Kusumah, R. Yayi Munara; Revi, Lestia; Kurniawati, Fitrianingrum
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 17, No 2 (2017): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.771 KB) | DOI: 10.23960/j.hptt.217147-155

Abstract

Characterize molecular the Nucleopolyhedrovirus (NPV) Hyposidra talaca Wlk. of tea plantation at Gunung Mas Bogor. Hyposidra talaca is one of the most important pest in tea plantation, and generally attacks of leaves and shoots. This pest cause yield loss up to 40-100%. NPV can be pathogenic to the pest of H. talaca and can be developed as an alternative measure to control H. talaca in tea plantations and based management appears to be more ecofriendly and effective. However, information regarding characterisation molecular of HytaNPV is limited. The study conducted to characterize molecular the NPV of H. talaca by restriction nucleotide and amino acid, by using gens lef-8.  Molecular identification used Polymerase Chain Reaction (PCR) consisted of DNA extraction, DNA amplication, and DNA electrophoresis. DNA amplication using gen lef-8 showed positif result with approximately 770 bp. Gen lef-8 can identified HytaNPV. DNA sequance showed that isolate HytaNPV Bogor had high homology of pathogonic NPV of genus Helicoverpa from Brazil, Australia, Spanyol and Netherland with homology nucleotide and amino acid reached 98% and 100%. Based on philogeny tree of HytaNPV was one group with pathogenic NPV of genus Helicoverpa.
Molecular Characters of AB-FAR Gene 1 of Aphelenchoides besseyi from Five Rice Varieties Fitrianingrum Kurniawati; Efi Toding Tondok; Yayi Munara Kusumah; Abdul Munif
Jurnal Fitopatologi Indonesia Vol 17 No 3 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.3.121-130

Abstract

Aphelenchoides besseyi merupakan nematoda penyebab penyakit pucuk putih yang terbawa benih padi. Gen AB FAR-1 diketahui sebagai gen penting yang mengendalikan patogenisitas A. besseyi. Penelitian dilakukan untuk mengetahui karakter gen AB FAR-1 yang diisolasi dari nematoda yang berasal dari benih padi. Ekstraksi nematoda dilakukan dengan metode corong Baerman dari benih 5 varietas padi “Ciherang“, “Inpari Sidenuk“, “Sintanur“, “Hibrida Prima“ dan “Pak Tiwi“. Ekstraksi DNA total nematoda menggunakan metode CTAB dilanjutkan dengan amplifikasi gen AB FAR-1 menggunakan primer spesifik FAR-F1/R1 dan analisis urutan nukleotidanya. Pita DNA spesifik gen AB FAR-1 berukuran 150 pb berhasil diamplifikasi dari semua sampel nematoda. Analisis sekuen menunjukkan bahwa gen AB FAR-1 tersebut memiliki homologi tertinggi (92.5 – 100%) dengan aksesi Genbank JQ686690.1, yaitu gen AB FAR-1 A. besseyi asal Cina. Walaupun memiliki homologi yang tinggi, terdapat beberapa perbedaan nukleotida pada sampel gen AB FAR-1 A. besseyi asal “Ciherang“, “Inpari Sidenuk“ dan “Hibrida Prima“. Analisis pohon filogenetika lebih lanjut mengelompokkan gen AB FAR-1 A. besseyi menjadi 2 grup, yaitu grup 1 terdiri atas gen AB FAR-1 A. besseyi asal Cina, “Sintanur“, “Hibrida Prima“ dan “Pak Tiwi“ dan grup 2 gen AB FAR-1 A. besseyi asal “Ciherang“, dan “Inpari Sidenuk“.
KEEFEKTIFAN BAHAN PELINDUNG ALAMI DALAM MEMPERTAHANKAN INFEKTIVITAS Spodoptera exigua NUCLEOPOLYHEDROVIRUS (SeNPV) Samsudin Samsudin; Teguh Santoso; Aunu Rauf; Yayi Munara Kusumah
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1932

Abstract

Spodoptera exigua nucleopolyhedrovirus (SeNPV) is a viral pathogen of onion caterpillar S. exigua with high pathogenicity. One of the major constraints to the use of SeNPV for biocontrol of onion caterpillar is its sensitivity to ultraviolet (UV) degradation. The purposes of this research were to determine the effect of sunlight exposure on the virulence of SeNPV and to find out the effective natural UV protectant to maintain the SeNPV virulence. The results showed that the sunlight radiation affects the SeNPV infectivity. Addition of 1% of coconut shell charcoal, lampblack, husk charcoal, yam flour, molasses, yam filtrate, turmeric filtrate and green tea filtrate to the SeNPV suspension were found to be effective as UV protectant. Coconut shell charcoal, lampblack and husk charcoal are activated carbon that can absorb UV light. Yam filtrate is a natural ingredient that contains saponins and is able to protect SeNPV particles as reflectance. While molasses, turmeric filtrate and green tea filtrate containing flavanoid serve as a protective virus particles and UV absorber.
Karakter morfologi, biologi, dan molekuler tiga isolat Helicoverpa armigera Nucleopolyhedrovirus (HearNPV) terhadap penggerek buah kapas Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) Farriza Diyasti; Teguh Santoso; R. Yayi Munara Kusumah
Jurnal Entomologi Indonesia Vol 13 No 3 (2016): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (509.92 KB) | DOI: 10.5994/jei.13.3.117

Abstract

Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) is one of the most important pests on cotton. Yield losses due to this pest can reach up to 70%. Pest control using the NPV has been proven to be effective in the field. However NPV isolates from different geographical locations could be showed variation in genetic and their biology. The purpose of this study was to compare morphology, biology, and molecular characteristics between three HearNPV isolates from cotton field in South Sulawesi, Central Java, and East Java. Scanning electron microscope (SEM) was used to look at morphological characters, whereas biological characters were observed by dipping shelled of young corn cob on each isolate suspension with a density of 2.5 x 103, 2.5 x 104, 2.5 x 105, 2.5 x 106, 2.5 x 107 POBs /ml and control then fed to the first instar larvae of H. armigera. Probit analysis was used to determine LC and LT. The molecular assay used polymerase chain reaction (PCR) with lef-8 gene. The results showed that the three isolates had the shape and size of polyhedra which was not significantly different with the mean diameter were 0.86 μm. The result showed that in 7 days post infection, HearNPV isolate from East Java was the most virulent isolates with the lowest LC50, which is 6.38 POBs/ml, but lowest LT50 value in 2.5 x 107 POBs/ml were showed both from East Java and South Sulawesi, which are 2.7 days post infection. Meanwhile, the DNA sequencing of the three isolates showed a high similarity (100% homology).
Keefektifan fosfin formulasi cair terhadap Aphis gossypii Glover dan Macrosiphoniella sanborni Gillette (Hemiptera: Aphididae) pada bunga potong krisan Nur Rachman; Dadang Dadang; R. Yayi Munara Kusumah
Jurnal Entomologi Indonesia Vol 12 No 3 (2015): November
Publisher : Perhimpunan Entomologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1123.493 KB) | DOI: 10.5994/jei.12.3.158

Abstract

Cut flower of chrysanthemum has high aesthetic and economic values. Export volume of chrysanthemums from Indonesia are lower than other countries, due to the impact of insect pest. Insect pests, Aphis gossypii Glover and Macrosiphoniella sanborni Gillette are currently associated with cut flowers especially chrysanthemum. One control measures usually taken in quarantine is fumigation. Alternative fumigant, liquified phosphine formulations may potentially be applied for quarantine treatment. The objectives of this study were to determine the concentration and exposure time of liquified phosphine against these species and to evaluate the effect of fumigant on the physical quality of cut flowers. The experiment was conducted in four steps: (1) identification and mass rearing of A. gossypii and M. sanborni; (2) preliminary tests on adult and third instar nymphs; (3) determination of liquified phosphine with various concentration and exposure time against adult and third instar nymphs of A. gosspypii and M. sanborni and; (4) validation test of effective concentration of fumigant and exposure time and the effect on quality of cut flowers. The results of study showed concentration of fumigant causing 100% mortality A. gossypii and M. sanborni  ere 500 ppm and 700 ppm, respectively with exposure time 12 hours. Concentrations of 700 ppm and exposure time up to 18 hours did not cause negative impact to the quality of chrysanthemum cut flowers.
Serangan Hama Belalang (Oxya spp.) pada Tanaman Talas (Colocasia esculenta L.) di Kelurahan Situ Gede Kecamatan Bogor Barat Kota Bogor Fahira Hanifah; Yayi Munara Kusumah
Jurnal Pusat Inovasi Masyarakat (PIM) Vol. 2 No. 5 (2020): Juli 2020
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (413.205 KB)

Abstract

Taro (Colocasia esculenta L.) is a plant that has many benefits for human life. Starting from the leaves to the tubers which are widely used as medicine and for food. It is undeniable that taro leaves have many benefits, but it is unfortunate that many farmers still lack the use of taro leaves due to pest damages. This study aims to determine the infestation of locust pests (Oxya spp.) on taro plants (Colocasia esculenta L.) in Situ Gede Village, West Bogor District, Bogor City, which is located in the land owned by Mr. Amran, one of the farmers there. The time of the research is 2 weeks, starting from 20 February 2020 until 3 March 2020. This research was conducted by survey method or by direct observation at the research location. The results of the study of the percentage of locust pest (Oxya spp.) on taro plants (Colocasia esculenta L.) in Situ Gede Sub-District, West Bogor District, Bogor City is 35.5%. Keywords: Oxya spp., Percentage, Symptoms, Taro
Deteksi dan Pembedaan Praktis Fitoplasma dari Beberapa Tanaman Inang Menggunakan PCR-RFLP Kikin Mutaqin; Purnama Hidayat; Budi Tjahjono; Yayi Munara Kusumah; Rusmilah Suseno
Jurnal Fitopatologi Indonesia Vol 19 No 5 (2023): September 2023 (IN PROGRESS)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.5.188-195

Abstract

Deteksi dan Pembedaan Praktis Fitoplasma dari Beberapa Tanaman Inang Menggunakan PCR-RFLP Fitoplasma adalah prokariota fitopatogenik dengan kisaran inang yang luas dan merupakan patogen yang perlu mendapatkan perhatian lebih banyak di Indonesia. Patogen ini relatif sulit untuk dideteksi dan diidentifikasi mengingat sifat biologinya yang rumit. Penelitian ini meliputi deteksi fitoplasma menggunakan teknik polymerase chain reaction (PCR) dengan primer P1/P7 dari tujuh tanaman bergejala, yaitu daun putih rumput Bermuda, kuning bambu, sapu kacang tanah, kedelai, kacang panjang dan kaktus, serta daun kecil ubi jalar. DNA gen 16S rRNA fitoplasma hasil amplifikasi PCR selanjutnya direaksikan dengan masing-masing enzim endonuklease restriksi AluI, RSaI dan MSeI untuk menghasilkan pola restriction fragment length polymorphism (RFLP). Tujuh tanaman sakit tersebut terkonfirmasi berasosiasi dengan fitoplasma yang ditunjukkan oleh produk PCR sebesar 1800 pb. Berdasarkan profil RFLP tiga enzim, fitoplasma dibedakan ke dalam dua kelompok, yaitu kelompok I (rumput Bermuda dan bambu) dan kelompok II (kacang tanah, kedelai, kacang panjang, kaktus dan ubi jalar). Fitoplasma kaktus merupakan sub-kelompok (galur) karena memiliki sedikit perbedaan fragmen dari profil RFLP dengan enzim MSeI.
Co-Authors A.A. Ketut Agung Cahyawan W Abdul Munif AUNU RAUF Budi Tjahjono DADANG DADANG Efi Toding Tondok Fahira Hanifah Farriza Diyasti Fitrianingrum Kurniawati, Fitrianingrum Kikin Mutaqin Nur Rachman Purnama Hidayat Revi, Lestia Rusmilah Suseno Samsudin Samsudin Samsudin Samsudin Teguh Santoso Teguh Santoso