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Quality of Yeast Extract of Industry Yeast Press Alcohol waste as Drying result with Various Temperature Variation Yusta, G. Maria; Meitiniarti, V. Irene; Kristiani, E.B.E.
Journal Of Natural Sciences And Mathematics Research Vol 2, No 2 (2016): Volume 2, Nomor 2, 2016
Publisher : Faculty of Science and Technology, State Islamic University Walisongo Central Java

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (952.257 KB) | DOI: 10.21580/jnsmr.2016.1.2.1664

Abstract

The content of yeast in industrial solid waste fermented beverages, has not been widely noticed and utilized, whereas many sources of nitrogen, vitamins and minerals are still present in this yeast. One of the utilization of waste yeast is processed into autolysis yeast extract. The drying process is often done using a spray dryer, but the process is costly so it is not efficient. In this study, we want to determine the temperature and drying time appropriate for maintain of yeast extract quality of yeast press. From this research, we concluded that a drying oven at 60 ° C for 6 hours is drying which does not damage the nutrient (protein, vitamine B2, carbohydrate, prolin, and lysin content) in yeast extract.©2016 JNSMR UIN Walisongo. All rights reserved.
DEKOLORISASI PEWARNA TEKSTIL SUMIFIX BLUE DAN REACTIVE RED 2 OLEH BAKTERI YANG DIISOLASI DARI LIMBAH INDUSTRI TEKSTIL Permatasari, Intan; Nugroho, Rully Adi; Meitiniarti, Vincentia Irene
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol 5, No 1 (2018): June 2018
Publisher : Badan Pengkajian dan Penerapan Teknologi (BPPT)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (593.563 KB) | DOI: 10.29122/jbbi.v5i1.2757

Abstract

Decolorization of Sumifix Blue and Reactive Red 2 Textile Dyes by Microbes Isolated from Textile Waste WaterAzo dyes represent the most commonly used group of dyes in textile industry and discharged into industrial effluents worldwide. Aims of this study are to isolate microbe from textile waste water and to determine their ability to decolorize Sumifix Blue and Reactive Red 2 textile dyes. Microbe was isolated from textile effluent of PT Timatex, Salatiga. The activity for decolorization was assayed by inoculating microbial isolates into dye containing medium. Living and nonliving cell were incubated in dye containing medium in order to determine if microbial cells involved in decolorizing dye. Five different microbial isolates have been isolated from textile waste water.  Isolates IBLTT_1 and IBLTT_5 showed the highest activity to decolorize Sumifix Blue, and only isolate IBLTT_1 showed the highest capability in decolorizing Reactive Red 2. Both isolates indicated positive potential towards biotreatment of textile waste water. Further results confirmed that decolorization was due to biodegradation, rather than physical adsorption by inactive cells.Keywords: decolorization, microbial isolation, Reactive Red 2, Sumifix Blue, textile effluent ABSTRAKPewarna azo mewakili kelompok pewarna yang umum digunakan pada industri tekstil dan banyak dijumpai di buangan limbah industri tekstil. Tujuan dari penelitian ini adalah untuk mendapatkan isolat dari limbah tekstil dan untuk mengetahui kemampuannya dalam mendekolorisasi pewarna tekstil Sumifix Blue dan Reactive Red 2. Sampel diperoleh dari limbah industri tekstil PT Timatex, Salatiga. Uji kemampuan dekolorisasi dilakukan dengan menginokulasikan isolat mikroba ke dalam medium Nutrient Broth yang mengandung pewarna. Untuk mengetahui apakah sel mikroba terlibat dalam dekolorisasi pewarna, maka sel hidup dan mati diinokulasi pada medium tersebut. Lima isolat yang berbeda diperoleh dalam penelitian ini. Isolat IBLTT_1 dan IBLTT_5 merupakan isolat dengan kemampuan dekolorisasi Sumifix Blue tertinggi. Isolat IBLTT_1 juga merupakan isolat dengan kemampuan dekolorisasi Reactive Red 2 tertinggi. Kedua isolat tersebut menunjukkan potensi positif terhadap pengolahan limbah tekstil. Hasil lebih lanjut menegaskan bahwa dekolorisasi Sumifix Blue dan Reactive Red 2 disebabkan oleh proses biodegradasi, bukan diadsorpsi oleh sel yang mati.Kata kunci: dekolorisasi, isolat mikroba, limbah tekstil, Reactive Red 2, Sumifix Blue
Pertumbuhan Enterococcus faecalis ID 6017 dan Kemampuan Dekolorisasi Beberapa Konsentrasi Orange II dalam Sistem Sinambung Kara, Patrisia Ilene; Meitiniarti, V. Irene; Timotius, K. H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.212 KB) | DOI: 10.24002/biota.v13i2.2671

Abstract

Orange II is the most used colorant in industries which involve coloring process. So far, Orange II decolorization process by Enterococcus faecalis ID 6017 under batch system has been proven to be able to decolorize orange to colourless. In the presence of intermediates accumulation in batch system, claimed bioprocess development with continues system. An hypothesis was carried out that continues system could reduce intermediates generated by Orange II degradation. This research aimed to know the growth of Enterococcus faecalis and its Orange II decolorization ability in the medium supplemented with Orange II with different concentration under continues system. The research was set on continues system with single cultures Enterococcus faecalis, grown in medium with 80, 120, or 160 mg/L Orange II concentration. Measured parameters were Orange II concentration, biomass, glucose concentration, intermediates compound (sulphanilic acid). It could be concluded that under continues system (D = 0.06 hour-1), Enterococcus faecalis could decolorize Orange II until 160 mg/l.
Pengaruh Penyulangan Medium yang Mengandung Orange II terhadap Pertumbuhan Enterococcus faecalis ID 6017 dan Kemampuan Dekolorisasinya Meitiniarti, V. I.; Sunardi, E. Vandiyani; Timotius, K. H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.362 KB) | DOI: 10.24002/biota.v11i1.2823

Abstract

The influence of fed Orange II containing medium on the growth of Enterococcus faecalis ID 6017 and its decolorization ability was studied in this research. A fed batch growth was compared with the batch growth. Total Orange II added to the both was 120 mg/L. In the fed batch, the Orange II was given into three steps. The first addition was at the initial culture medium. The second and third were added after the almost total decolorization or clearance of the Orange II given in the first or the second addition respectively. The culture was incubated under static condition and room temperature. The fed batch growth was better than the batch growth, seen from both aspects; their growth parameters and the decolorization ability. The biomass yield and specific growth rates of the fed batch was higher than the batch growth. Under fed batch growth, the decolorization was 85-94%, while the batch growth was only 54%. For achieving these performances, the fed batch growth was needed to consume more glucose.
PENGURANGAN AMONIUM DENGAN METODE NITRIFIKASI DAN ANAMMOX PADA AIR LINDI DARI TEMPAT PEMBUANGAN AKHIR SAMPAH JATIBARANG, SEMARANG Nindrasari, Gabriela; Meitiniarti, V. Irene; C. Mangimbulude, Jubhar
Prosiding Seminar Biologi Vol 8, No 1 (2011): Seminar Nasional VIII Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (304.599 KB)

Abstract

ABSTRAK Kandungan amonium yang cukup tinggi dalam air lindi TPA Jatibarang dapat menurunkan kualitas air sungai Kreo sehingga perlu diturunkan kadarnya. Kadar amonium secara biologis dapat diturunkan menggunakan proses nitrifikasi-denitrifikasi dan anammox. Penelitian ini dilakukan dengan tujuan memanfaatkan prinsip proses nitrifikasi dan anammox yang dirancang secara simultan dalam reaktor tidak terpisah, yang ditandai dengan adanya aktivitas dan interaksi bakteri nitrifikasi dan anammox untuk menurunkan jumlah amonium pada air lindi. Dalam penelitian ini digunakan metode kultur batch pada medium air lindi yang dirancang dengan zonasi aerob-anaerob untuk lingkungan perkembangan bakteri nitrifikasi dan anammox. Kadar amonium, nitrit, dan nitrat pada medium selama 28 hari diukur dan dianalisis dengan spektrofotometri sebagai parameter nitrifikasi, sedangkan kadar amonium dan nitrit diukur sebagai parameter anammox. Proses anammox berlangsung selama 14 hari pertama, dimana indeks amonium adalah 1.0 dan indeks nitrit 0.9, sedangkan pada hari ke-14 hingga hari ke-28 proses denitrifikasi terjadi dengan indeks amonium adalah 0.6 dan nitrit adalah 10.6. Hasilnya adalah kadar amonium pada kultur mengalami penurunan dengan nilai efisiensi zona aerob 39.75% dan zona anaerob 56.35% sehingga dapat disimpulkan bahwa terjadi proses nitrifikasi dan anammox pada medium air lindi. Kata kunci : pengurangan amonium, anammox, air lindi, TPA Jatibarang.
Pertumbuhan Curah Enterococcus faecalis Id 6017 dan Kemampuan Dekolorisasi Reactive Red-2 pada Medium yang Mengandung Gliserol Meitiniarti, V. I.; Napitupulu, Morina M.; Timotius, K. H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2004): February 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.63 KB) | DOI: 10.24002/biota.v9i1.2829

Abstract

Enterococcus faecalis ID 6017 can utilize glycerol as the source of carbon and energy for its growth. The present of glycerol in the medium containing Reactive Red-2 not only influenced its growth but also its ability to decolorize Reactive Red-2. The aim of this study was to investigate the growth of  Enterococcus faecalis (E. faecalis) and its ability to decolorize Reactive Red-2. The microbe was grown in batch system with three different growth medium, i.e. medium which contained (i) 1.643 g/l glycerol and 0.08 g/l reactive red-2, (ii) 1.643 g/l glycerol, and (iii) 0.08 g/l Reactive Red 2. The result of this study showed that the growth of  E. faecalis and its ability to decolorize Reactive Red-2 on medium contained glycerol was better than without glycerol. E. faecalis could not growth and decolorized Reactive Red-2 on medium without glycerol.
Astaxanthin Production by the Red Yeast (Phaffia rhodozyma), Grown on Yeast Extract Addes Coconut Water K H Timotius; Ana W Purnomo; V I Meitiniarti
Jurnal Teknologi dan Industri Pangan Vol. 14 No. 2 (2003): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2725.432 KB)

Abstract

This experiment was conducted to evaluated the effect of yeast extract addition in fresh green coconut water medium during the batch growth and astaxanthin production of Phaffia rhodozyma MUCL 31142. The addition of yeast extract (0,025% and 0,075%) could increase the cell growth (37,12 g/l and 49,18 g/l), growth rate (0,061/hour and 0,074/hour), total production of astaxanthin (4,871 mg/l and 9,442 mg/l), specific concentration of astaxanthin (118,99 µg/g biomass), production rate of astaxanthin (0,042/hour and 0,088/hour), astaxanthin yield (0,236 mg/g glucose and 0,342 mg/g glucose, and glocose consumption (19,84 g/l and 26,95 g/l). Key Word : Astaxanthin, Phaffia rhodozyma, coconut water.
Optimum Concentration of Glucose and Orange II for Growth and Decolorization of Orange II by Enterococcus faecalis ID6017 under Static Culture V. IRENE MEITINIARTI; ENDANG S. SOETARTO; EKO SUGIHARTO; KRIS HERAWAN TIMOTIUS
Microbiology Indonesia Vol. 2 No. 2 (2008): August 2008
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.255 KB) | DOI: 10.5454/mi.2.2.5

Abstract

Growth and decolorization performance of bacterial grown on azodyes-containing-medium is influenced by various concentrations of carbon sources and azodyes. The optimum level of glucose and Orange II concentration for growth and Orange II decolorization by Enterococcus faecalis ID6017 are reported in this paper. The experiments were carried out in liquid static culture as batch experiments. Glucose and Orange II concentrations used in these experiments were 0.45, 0.90, 1.80 g l-1, and 40, 80, 120 mg l-1, respectively. The specific growth rate and decolorization rate of Orange II by E. faecalis were highest on the medium which contained at least 0.90 g l-1 glucose. It is necessary to note that glucose above 0.90 g l-1 gave no significant difference. On the medium containing 0.90 g l-1 glucose and 80 mg l-1 Orange II, E. faecalis grew with the highest specific growth rate (0.28 h-1) and Orange II decolorization rate (0.47 h-1). The maximum specific growth rate of biomass (μmax) and the halfsaturation coefficient (KS) under optimal conditions were 0.25 h-1 and 1.5 g.l-1, respectively. The kinetics of decolorization indicated that the process followed first order kinetics with respect to the initial concentration of Orange II. The inhibition constant (KI) was found to be 750 mg l-1 Orange II, indicating that Orange II concentration at e” 750 mg l-1 would inhibit bacterial growth to decolorize Orange II..
Products of Orange II Biodegradation by Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 VINCENTIA IRENE MEITINIARTI; ENDANG SUTARININGSIH SOETARTO; KRIS HERAWAN TIMOTIUS; EKO SUGIHARTO
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (261.229 KB) | DOI: 10.5454/mi.1.2.1

Abstract

Chryseobacterium indologenes and Enterococcus faecalis were isolated from activated sludge of textile wastewater treatment plant. These bacteria had the ability to decolorize several azo-dyes. Degradation of azo dyes was initiated by decolorization (reduction of azo bond) which occurred in anaerobic condition. In this study, we focussed on biodegradation of Orange II by pure culture of C. indologenes ID6016 and E. faecalis ID6017, and to determine the metabolite products of Orange II degradation. The degradation of Orange II by both bacteria was carried out in batch experiments using liquid medium containing 80 mg/l Orange II, under sequential static agitated incubation. During the bacterial growth under static incubation (6 h), 66.1 mg/l Orange II were decolorized by 35.54 mg/l biomass of E. faecalis ID6017, but no decolorization found with C. indologenes ID6016. Based on HPLC results, the decolorized Orange II products were identified as sulfanilic acid and amino-naphthol. These metabolites were probably used or degraded by C. indologenes ID6016 under agitated incubation.
Decolorization of Orange II by Mixed Culture of Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 VINCENTIA IRENE MEITINIARTI; KRIS HERAWAN TIMOTIUS; ENDANG SUTARININGSIH SOETARTO; EKO SUGIHARTO
Microbiology Indonesia Vol. 6 No. 3 (2012): September 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.238 KB) | DOI: 10.5454/mi.6.3.4

Abstract

Previous work showed that Enterococcus faecalis ID6017 and Chryseobacterium indologenes ID6016 were able to decolorize the orange II qualitatively. In that experiment, E. faecalis could decolorize orange II more rapidly than C. indologenes. The objective of this study was to examine the decolorization of orange II by mixed culture and the growth of both bacterial species on orange II containing medium. The experiment was done in 500 mL sterilized Erlenmeyer flasks containing 285 mL growth media with 80 mg L-1 orange II. Five different treatments were performed in this project, i.e. medium was inoculated with (i) 15 mL of sterile aquadest, (ii) 15 mL of C. indologenes, (iii) 15 mL of E. faecalis, (iv) 7.5 mL of C. indologenes and 7.5 mL of E. faecalis, and (v) 7.5 mL of E. faecalis until decolorization occured , followed by inoculation with 7.5 mL of C. indologenes. Bacterial growth (total cells number), orange II, glucose, and suphanilic acid, as intermediate product of orange II decolorization, concentrations were measured every 2 h.The maximum decolorization of orange II was observed in the medium inoculated with a mixed culture of E. faecalis and C. indologenes. Decolorization of orange II occurred of growth and gave final concentration of sulphanilic acid of 7.06 mg L-1. During culture both species grow in equilibrium in terms of population.