Claim Missing Document
Check
Articles

EFEK SITOTOKSIK EKSTRAK ETANOLIK HERBA SELEDRI (Apium graveolens L.) PADA SEL KANKER T47D, WiDr, DAN HeLa Palupi, Kartika Dyah; Wulandari, Ainun; Goenadi, Fina Aryani; Nur, Kholid Alfan; Fitriasari, Aditya; Meiyanto, Edy
Farmasains : Jurnal Farmasi dan Ilmu Kesehatan Vol 1, No 2 (2011): Oktober 2010 - Maret 2011
Publisher : University of Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.379 KB) | DOI: 10.22219/far.v1i2.1164

Abstract

Celery (Apium graveolens L.) is commonly used to lower blood pressure, antirheumatic, relaxant, mild diuretic, antiseptic for the urinary tract, antioxidant, and anti-inflammation. According to previous studies, a number of the phytochemicals found in the plant show cytotoxicity toward some types of cancer cells. However, studies on the cytotoxic effects of celery herb ethanolic extract (CEE) on breast cancer cell (T47D), colon cancer cell (WiDr), and cervix cancer cell (HeLa), however, has not been done yet. Our research aims at doing so. Cytotoxicity test was conducted using MTT assay and its absorbance was read using ELISA reader at λ = 595 nm. Results of the assay show that CEE reduces cell viability at concentrations of 100-750 µg/ml on HeLa cells, while reduction of T47D and WiDr cell viability was not achieved until concentrations of 500-750 µg/ml. Based on these results, we conclude that CEE hold many potentials for further developments as preventive and therapeutive agent in cancer treatment.
Structure Modification of Ethyl p-methoxycinnamate Isolated from Kaempferia galanga Linn. and Citotoxicity Assay of The Products on WiDr Cells Ekowati, Juni; Rudyanto, Marcellino; Sasaki, Shigeru; Budiati, Tutuk; Sukardiman, .; Hermawan, Adam; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Ethyl p-methoxycinnamate, major ingredient of Kaempferia galanga rhizome, have been reported not only has analgesic – anti inflammatory activities like NSAIDs which inhibited cyclooxygenase, but also inhibit tumor cell proliferation in specimen of mouse epidermis. Therefore, it will be interesting to carry out  synthetic studies on the derivates of ethyl  p-methoxycinnamate and searching their citotoxic activity on WiDr cell. We wish to report of structure modification on carboxyl moiety of  ethyl p-methoxycinnamate  and  evaluation on their citotoxic activity  on WiDr cell. Isolation of ethyl p-methoxycinnamate from Kaempferia galanga rhizome was carried out by percolation with ethanol 96% as solvent. Hydrolysis of ethyl p-methoxycinnamate in basic condition was performed to obtain p-methoxycinnamic acid. Preparation of some thiourea derivates of ethyl  p-methoxycinnamate was carried out  by microwave irradiation. Citotoxicity assay was carried out by MTT method for 48 h.   Modification  of  carboxyl  group  of  ethyl  p-methoxycinnamate to its thiourea form could be carried out by microwave irradiation gave; (E)-3-(4-methoxyphenyl)-N-(phenylcarba- mothioyl)acrylamide (50%); (E)-3-(4-methoxyphenyl)-N-(4-methoxyphenylcarbamothi- oyl)acrylamide (26%) and (E)-3-(4-methoxyphenyl)-N-(4-methylphenylcarbamothioyl) acrylamide (54%), yield calculated for 2 step from the acid chloride. All compounds showed no citotoxic effect on WiDr cell at 48 h incubation.
SynergisticCombinationofCiplukan(Physalis angulata) HerbsEthanolicExtractandDoxorubicinonT47DBreast CancerCells Armandari, Inna; Palupi, Kartika Dyah; Farida, Sofa; Hermawan, Adam; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Doxorubicinisoneofchemotherapeuticagentwidelyusedinbreastcancertreatment,but in high dose doxorubicin gives negative side effect, including vomit, nausea, immune suppression, and cardiac toxicity. This toxicity hopefully could be reduced by combination chemotherapy using natural herbs such as ciplukan herb. This research was conducted to explorecytotoxicactivityofsingleciplukanherbsethanolicextractanditscombinationwith doxorubicinonT47Dbreastcancercells.Cytotoxicactivityofciplukanherbsethanolicextract only and its combination with doxorubicinwere tested on T47D cells using MTT assay toobtainIC50valueandcombinationindex(CI),respectively.Singleextractshowedcytotoxic activityonT47DcellswithIC50valueofwas160*g/ml.Thus,combinationtreatmentfrom ciplukanherbsethanolicextractanddoxorubicinshowedsynergisticeffect(CI<1,0).Thiseffect wasreachedatconcentrationofciplukanherbsethanolicextract-doxorubicin80μg/ml-2nM, 80 μg/ml-4 nM, and 80 μg/ml-8 nM. This research indicated that ciplukan herbs ethanolic extractispotentialtobeappliedasco-chemotherapeuticagentinbreastcancertherapy.
Leunca (Solanum nigrum L.) Herbs Ethanolic Extract Increase Cytotoxic Activity of Cisplatin on Hela Cervical Cancer Cells Istiaji, Raditya Prima; Fitria, Maya; Larasati, .; Tjondro, Fortunella; Maruti, Astrid Ayu; Setyowati, Erna Prawita; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 1 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Cervical cancer is one of leading causes of cancer death in women in the developing countries. The use of cisplatin as chemotherapy agent in cervical cancer is known to cause side effects and also resistance for long-term uses. One of the strategies to prevent cervical cancer based on combination agents is being developed. Leunca (Solanum nigrum L.) has been revealed to inhibit growth of human cancer cells. Therefore, it can be used in combination with cisplatin to reduce those side effects and prevent the occurrence of cell resistance. Ethanolic extract of Leunca Herb (ELH) and cisplatin were tested their cytotoxic effect on HeLa cervical cancer cell by using MTT assay to determine IC50 value. The combinationss of cisplatin-ELH were tested to determine the combination index (CI value).  The IC50 of ELH and cisplatin on HeLa cells were 227 µg/mL and 17 µM. rRespectively. Tthe study of combination resulted that almost all the index combinations were <0,9 showed  the effect of synergism combination. The Ooptimum concentration of combination was  1/8 IC50 cisplatin–1/8 IC50 ELH. The results indicated that ELH had a potency to be combination agent to enhance the activity of cisplatin on HeLa cervical cancer cells. Therefore, further study on its molecular mechanism needs to be explored.
Combination of Solanum nigrum L. Herb Ethanolic Extract and Doxorubicin Performs Synergism on T47D Breast Cancer Cells Anindyajati, .; Sarmoko, .; Putri, Dyaningtyas D. P.; Hermawan, Adam; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Leunca (Solanum nigrum L.) has been proven to possess  anticancer activity on some type of cancer cells. In vitro study of solamargine found in the herb showed cytotoxic effect against several breast cancer cell lines, such as T47D and MDA-MB-31. Hence, further study on its potential as a co-chemotherapeutic agent needs to be conducted, in order to overcome resistance problem commonly found in cancer  chemotherapy. This study aimed to examine the cytotoxic activity of leunca herb ethanolic extract (LEE) alone and its combination with doxorubicin. Single and combinational treatment of LEE and doxorubicin on T47D breast cancer cells were done, and their viability representing cytotoxicity were analyzed by using MTT assay to determine the IC50 value and combination index (CI) to evaluate the combinational effect.  Twenty four hours-treatment of LEE  alone gave cytotoxicity activity showing a dose-dependent manner with the IC50 of 47 µg/ml, while combinational treatment showed that 4 µg/ml LEE was found to be synergist with 4 nM doxorubicin on T47D cells, with the optimum CI value of 0.59. This result shows that Solanum nigrum L. is potential to be proposed as doxorubicin co-chemotherapeutic agent against breast cancer. Further study on its molecular mechanism needs to be conducted.
Ethanolic Extract of Moringa oleifera L. Increases Sensitivity of WiDr Colon Cancer Cell Line Towards 5-Fluorouracil Nur, Kholid Alfan; Putri, Herwandhani; Cahyani, Fany Mutia; Katarina, Aulia; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

For more than four decades, combination chemotherapy (co-chemotherapy) has been employed as a means to increase the effectiveness of chemotherapy regiments. The aim of our research is to investigate the activity of  Moringa oleifera  L. (tanaman kelor) ethanolic extract (MEE) as a co-chemotherapy agent with 5-fluorouracil (5-FU) on WiDr colon cancer cell line. Evaluation of MEE potency as a co-chemotherapy agent with 5-FU was based on cytotoxic activity based on percent cell viability via MTT  assay, and based on apoptosis observation via the double staining method using acrydin orange – ethidium bromide (AE) as the staining reagent.Cytotoxicity evaluation of single treatment using concentrations of 5, 20, 50, 100,125, and 250 µg/ml of MEE reduced cell viability 24 hours post-treatment. 5, 50, and 250 µg/ml of MEE was chosen as the combination concentrations with 1000 µM 5-FU. MTT assay 24 hours and 48 hours post-combination treatment showed significant cell viability reduction in comparison to those of single treatments. Apoptosis observation using the double staining method shows the presence of apoptotic cells 48 hours post combination treatment. MEE is a potential co-chemotherapy agent  by increasing the sensitivity  of WiDr colon cancer cell line towards 5-FU.
Taraxacum officinale Leaves Ethanolic Extract as Immunostimulatory Agent For Reducing Side Effect of Doxorubicin in Sprague Dawley Rats Kasianningsih, Sri; Rivanti, Erlina; Pratama, Ratih Hardika; Pratama, Nanda Resa; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Doxorubicin as chemotherapeutic agent causes immunosuppresive. The aim  for this study to determine the effect of ethanolic extract of Taraxacum oficinale (ETO) in immunity system of Sprague Dawley  rat that induced  by doxorubicin to observe the profile of immunity cells. Sprague Dawley rats were divided into five groups each groups contain five rats : control doxorubicin group, doxorubicin dose 4,67 mg/kgBW+ ETO dose 1000 mg/kgBW, doxorubicin dose 4,67 mg/kgBW+ ETO dose 500 mg/kgBW, control extract group, and without treatment. Then the number of leukocytes, lymphocytes and neutrophils were analyzed  by hematology analyzer, whereas CD8+ T lymphocytes by flowcytometry. Results showed groups of  doxorubicin combined with ETO dose 1000 mg/kgBW and 500 mg/kgBW increased the number of leukocytes, lymphocytes, neutrophils,  cytotoxic CD8 + T cells  T cells compared  to control doxorubicin group. These data presents that etanolic extract of Jombang leaves has  immunostimulatory activity and potential as co-chemotherapy agents. Molecullar mechanism underlaying it’s immune activity need to be explored in detail.
Banana Peels (Musa paradisiaca L.) Extract as Phytoestrogen on Ovariectomized Mice Mammary Gland Development by Inducing c-Myc Expression Pratama, Nanda Resa; Gilang, Yurista; Riata, Rita; Hermawan, Adam; Ikawati, Muthi; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Hormone Replacement Therapy (HRT) is therapy for estrogen deficiency and post menopausal  syndromes,  but  high  cost  and  unwell-secured  therapy.  One  of  alternative therapy  is  the  usage  of  phytoestrogens.  The  banana  peel  contains  flavones,  flavonol, flavanone and polimethoxyflavone which are potential as phytoestrogen. The purpose of this study was to examine the estrogenic effect of banana peel extract (BPE) development of mammary gland of ovariectomized rats. Estrogenic effects was examined based on in vivo and in silico experiment. For in vivo experiment, female Sprague-dawley rats aged 50 days were ovariectomized. At 70 days of age, 12 rats were treated with BPE 500 mg/kgBB and 1000mg/kgBB, 5 rats were treated with estradiol 2g/day while others served as control were treated with CMC-Na 0.5% and sacrificed 2 weeks later. The base line ovariectomized rats and base line non-ovariectomized rats were sacrificed at 70 days of age. The in silico experiment examined by molecular docking between myricetin and estrogen receptor alpha (ER-α). The result of in vivo experiment showed that 1000 mg/kgBW BPE induced c-Myc expression  and  enhance  ovariectomized  rat  mammary  gland  development  significantly. Meanwhile, molecular docking showed that there are hydrogen bond interaction between bioactive compound in BPE and Estrogen Receptor (ER)-α but less powerfull than estrogen and ER-α interaction. In summary, BPE can act as an estrogen agonist,  resulting in the enhancement of c-Myc expression. 
Antiproliferative Activity of Ethanolic Extract of Ciplukan Herbs (Physalis angulata L.) on 7,12-Dimethylbenz[a]nthracene-Induced Rat Mammary Carcinogenesis Monikawati, Ameilinda; Farida, Sofa; Putri, Laras Widawaty; Ikhtiarsyah, Yurista Gilang; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Physalis angulata  L.  is  an  annual  herb  widely  used  as  popular  medicine  for  the treatment of cancer. Physalis angulata L. ethanolic extract (PEE) has been demonstrated to have strong cytotoxic activity against breast cancer, inhibited cancer cell’s proliferation and induced  cell  cycle  arrest.  The  aim  of  our  study  is  to  investigate  the  effect  of  PEE  as  a cancer  chemopreventive  agent  on  7,12-dimethylbenz[a]nthracene  (DMBA)-induced  rats mammary. The antiproliferative activity was characterized by monitoring the histopatology representation  and  expression  of  cell  proliferation  on  DMBA-induced  mammary  rats  that were  treated  with  PEE  against  control  groups.  The  histopatology  representation  were analyzed  by  Haematoksilin  Eosin  (HE)  staining  method,  while  proliferative  activity  was detected by AgNOR method. The HE staining results showed significant differences in cells morphology  of  treatment  groups  compared  to  the  control  groups.  Thus  results  suggest that  PEE  was  able  to  repair  morphology  of  cells  undergoing  carcinogenesis.  AgNOR method  showed  decreasing  occurrence  of  black  dots  between  treatment  and  control groups. Thus, we conclude that PEE has an antiproliferative activity on DMBA-induced rat mammary.  Therefore,  the  ethanolic  extract  of  Physalis  angulata  herbs  is  a  potential chemopreventive agent on cancer. Further study on its molecular mechanism needs to be explored. Keywords:  Physalis  angulata,  breast  cancer, 7,12-dimethylbenz[a]nthracene, carcinogenesis, antiproliferative
Hesperidin Increases Cytotoxic Activity of Doxorubicin on Hela Cell Line Through Cell Cycle Modulation and Apoptotis Induction Kusharyanti, Indri; Larasati, .; Susidarti, Ratna Asmah; Meiyanto, Edy
Indonesian Journal of Cancer Chemoprevention Vol 2, No 2 (2011)
Publisher : Indonesian Research Gateway

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4.399 KB)

Abstract

Combination of chemotherapeutic agent and chemo preventiveagent is being a new approach in cancer treatment.This is aimed at enhancing the effectivity and also reducing drugresistance and adverse side effect of the chemo therapeuticagent.Hesperidin,acitrus flavonoid has reported to reduce theproliferation of many cancer cells.The objectives of this study were to investigate cytotoxic activities, cell cycle modulation and apoptosis induction of he speridinand its combination withdoxorubicinon Helacelllines.MTT [3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazoliumbromide] assay was used tomeasure the growth inhibitory effect of he speridinanditscombination with doxorubicinon Helacells.Cellcycle profile was determined by flowcytometry and the dataobtained was analyzed by using Mod Fit LT3.0program.Apoptos is assay was done using double staining method usingethidium$bromideandacridine$orange.Hesperidin inhibited cellgrowth with IC5048M, while the IC50 of doxorubicin was 1000nM.Combination of 500n Mdoxorubicin and 6M hesperidin showed strongest inhibitory effect toward Hela cells. Hesperidin of 24 2M accumulated HeLacells at G1phase,butit scombinationwith 500nM Doxorubicin gave G1 and Sphase accumulation at 24h incubation.Both of Hesperidin and Doxorubicin were capable of inducing apoptosis.Inaccordance of the apoptoticeffect,hesperidin,doxorubicin and their combination decreasedthe expression Bcl$2 and increased the expression of Bax. Accordingtothisresult,hesperidinhasapotencytobedevelopedasco$chemotherapeutic agent forcervical cancer. Key    words:Cochemotherapy,Hesperidin,Doxorubicin,Hela,MTTassay
Co-Authors . Anindyajati . Larasati . Sugiyanto . Sukardiman Adam Hermawan Aditya Fitriasari Agung Endro Nugroho Agusta Fauzi, Ilham Ahmad Fudholi Ainun Wulandari Alexxander, . Alexxander, . Ameilinda Monikawati Andita Pra Darma Arief Nurrochmad Arief Rahman Hakim Astrid Ayu Maruti Aulia Katarina Ayu Maruti, Astrid B. Sudarto D., Andita Pra D., Andita Pra Daâ??i, Muhammad Da’i, Muhammad Dewi Arum Sekti, Dewi Arum Dewi Pamungkas Putri, Dyaningtyas Dewi Pratiwi Djaswadi Dasuki Dwi Ana Nawangsari, Dwi Ana Dwi Nurahmanto Dyaningtyas D. P. Putri Dyaningtyas Dewi Putri Pamungkas Endah Puspitasari Endang Purwaningsih Erlina Rivanti Erna Prawita Setyowati Fany Mutia Cahyani Feby Handoko, Fransiscus Fikri Amalia Fina Aryani Goenadi, Fina Aryani Fitria Rahmi Fiveri, Anis Fiveri, Anis Fortunella Tjondro Herwandhani Putri Ibrahim Arifin Ika Nurzijah Ika Rahmawati Sutejo Ilham Agusta Fauzi Imono Argo Donatus, Imono Argo Indri Kusharyanti Inna Armandani, Inna Inna Armandari Iwan Sahrial Hamid JAKA WIDADA Jenie, Riris Istighfari Juni Ekowati Kadarsih Soejono, Sri Kadarsih Soejono, Sri Kartika Dyah Palupi Kholid Alfan Nur Laras Widawaty Putri Luthfia Indriyani M, Kawaichi M, Kawaichi Mae Sri Hartati Wahyuningsih, Mae Sri Hartati Marcellino Rudyanto Maria Dwi Supriyati, Maria Dwi Masashi Kawaichi, Masashi Maury, Hendra Kurniawan Maya Fitria Muhammad Da'i Muhammad Da’i, Muhammad Muhammad Fithrul Mubarok, Muhammad Fithrul Muthi Ikawati N., Perdana Adhi N., Perdana Adhi Nanda Resa Pratama Niken Nur W, Niken Novi Hastuti, Novi Nunuk Aries Nurulita Nurma Sabila P.K.W., Diah Ayu P.K.W., Diah Ayu Perdana Adhi Nugroho R A Susidarti Raditya Prima Istiaji Rahmi Khamsita Ratih Hardika Pratama Ratna Asmah Susidarti Retno Murwanti Riris I Jenie, Riris I Riris Istighfari Jenie Riris Istighfari Jenie Rita Riata Rosa Adelina Rosana Anna Ashari, Rosana Anna Rosye H.R. Tanjung Rul Afiyah Syarif Sari Haryanti Sarmoko Sarmoko Sendy Junedi, Sendy Sendy Junedy, Sendy Septriyanto Dirgantara Shigeru Sasaki Sitarina Widyarini Sofa Farida Sri Handayani Sri Kadarsih Soejono Sri Kasianningsih Sri Susilowati Sri Tasminatun, Sri Sugeng Riyanto Sugiyanto . Sugiyanto . Supardjan A. M. Supardjan A.M., Supardjan Supardjan AM, Supardjan Susi Ari Kristina Tutuk Budiati Umar A. Jenie Umar Anggara Jenie Umar Anggara Jenie Yundari, Yundari Yurista Gilang Yurista Gilang Ikhtiarsyah Yuyun Farida, Yuyun