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All Journal BERITA BIOLOGI Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology
Apridah Cameliawati Djohan
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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ANALISIS KARBOKSIMETIL SELULOSA DARI BAKTERI Acetobacter xylinum DAN Acetobacter sp. RMG-2 Ruth Melliawati; Apridah Cameliawati Djohan
BERITA BIOLOGI Vol 12, No 3 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i3.642

Abstract

Bacterial cellulose has long been manufactured and used for industrial purposes and health. Bacterial cellulose more profitable than the cellulose plants because during the manufacturing process, they do not harm the environment. The purpose of this study was to identify differences of cellulose produced by Acetobacter xylinum and Acetobacter sp. RMG-2 from that produced by plants. The study was also aimed to determine superiority of carboxymethyl cellulose (CMC) produced by those bacteria. The medium HB was prepared for the production of cellulose from both bacteria. Bacterial cellulose preparation was carried out to obtain solid fine powder, followed by manufacturing carboxymethyl cellulose through several stages to obtain CMC powder. CMC analysis was performed for both bacteria and plants targeted on the surface structure of cellulose, the density of solids, viscosity CMC and functional groups. As a result, the surface fiber cellulose plants had a wider space than fiber cellulose bacterium. The density of solids of CMC A. xylinum, A. sp. RMG-2 and plant were 30.9998 g/cm3, 0.0079 g/cm3 and 0.9978 g/cm3 respectively. Viscosity of the CMC were of 5.78 cP, 5.25 cP and 5.91 cP for each A. xylinum,A. sp. RMG-2 and plant. CMC functional groups of bacteria has met the parameters of success as indicated by the infrared spectrum since it formed a methyl group, carboxyl group and the group of sugar. Cellulose Acetobacter sp. RMG-2 and A. xylinum cellulose can replace plants through the process of compound alkalization with sodium hydroxide, because the compound can lower the level of density of pores of cellulose fibers. The CMC resulting from bacterial cellulose as good as CMC plant and had characteristics resembling CMC plant.
Cloning of Partial β-Mannanase Gene from Indonesia Marine Bacteria Bacillus Subtilis LBF-005 Yopi yopi; Nanik Rahmani; Maghfirotul Amaniyah; Apridah Cameliawati Djohan
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 12, No 1 (2017): May 2017
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v12i1.252

Abstract

 The strain LBF-005 from marine bacteria have already isolated and screened for mannanase degrading enzyme in submerged fermentation process. This strain was further identified by using 16S rRNA showed that bacterium is belong to Bacillus subtilis that could produce mannanase with activity around 9.5 U/mL. The optimum pH and temperature for the activity of crude enzyme for mannanase was 6.0 and 50 oC. Cloning of mannanase gene from B. subtilis was conducted using six primers set designed based on the homology analysis conserve region several mannanase from bacteria (Bacillus sp.) glycosyl hydrolase (GH) family 26. Optimization of PCR conditions was performed by gradient PCR to obtained PCR product of b-mannanase gene. The PCR product was obtained by third primer combination and was estimated to be around 972-bp. Analysis of the nucleotide sequence showed that sequences has similarity with mananase gene from other Bacillus sp., such as the B. subtilis strain WLY-12, B. subtilis strain WL-8, B. subtilis strain CICC 10260, B.subtilis strain CD-25, B.subtilis strain G1, and Bacillus sp. SWU60 were about 98%, 98%, 98%, 98%, 97% and 95%, respectively. The next research will to obtain a whole gene encoding β-mannanase by using in vitro cloning method, characterization of recombinant mannanase and application this enzyme for mannooligosaccharides production.
Co-Authors Maghfirotul Amaniyah Nanik Rahmani Nanik Rahmani Ruth Melliawati Yopi yopi