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EFEKTIVITAS BAKTERI PROBIOTIK DARI UDANG GALAH (Macrobrachium rosenbergii, de Man) TERHADAP BAKTERI PATOGEN Aeromonas hydrophila, Pseudomonas stutzeri DAN Vibrio alginolyticus Desi Anggreni; Feliatra '; Nursyirwani '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractProbiotic bacteria capable of producing substances like antibiotic sandin dispensable for being able to suppressthe growth of pathogenic bacteria into disease and parasites in aquaculture. The research purposeof this study to find probiotic isolates were able to controlthe disease in aquaculture. The candidate probiotic bacteria isolated from prawn (Macrobrachium rosenbergii, de Man) by the method of scratch on TSA and NA media. Anti-bacterial activity using media discs on Zobell and TSA. 4 isolates were found which consisted of coccus, white and orange colonies. All isolates had inhibitory effect onthe growth of pathogenic bacteria. The highest sensitivitytest is indicated by P4 DUG isolates of A.hydrophilla at13,30, 13,20 and14,10 of P.stutzeri in V.alginolyticusKeyword : Probiotic, Effectiveness, Tiger Prawn.
The Effect of space planting on Growth of Mangrove (Avicennia marina) Seedlings in The Anak Setatah Village, West Rangsang District Meranti Archipelago Riau Province Ari yanto rama doni; Syafruddin Nasution; Feliatra '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted in Febuary - April 2016 at Anak Setatah Village, West Rangsang District Meranti Archipelagos Riau Province. This study aims to determine the effect of spacing on seedling height, survival rateand leave number of A. marina. The method used was experiment method. Planting space treatment were 25, 50 and 75 cm. The result for 60 days of observation show that the best space in high found in plant spacing 75 cm with a value of 26,04 cm/60 days, and the best growth for diameter was at space of 25 cm, with a stem diameter growth of 0.41 cm/60 days. The highest number of leaves in a spacing of 75 cm. The Anova test results that spasing planting given effect to height grow, stem diameter, and total number of leafs A. marina. Student- Newman- Keuls test show that treatment 25 cm is diffirent with 50 cm, and 75 cm, but treatment 50 cm is not diffirent with 75cm.Keywords: Avicennia marina, growth, and planting space.
THE BACTERIOCIN ANTIMICROBIAL TEST ACTIVITY OF PROBIOTIC BACTERIA ISOLATED FROM GIANT PRAWNS (Macrobrachium rosenbergii) Popy Wardani; Feliatra '; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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This research was conducted to test the antimicrobial activity of bacteriocin isolates of probiotic bacteria isolated from giant prawns (Macrobrachium rosenbergii). The method used was experiment method with tested the inhibition against pathogenic bacteria (Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri). The results showed that bacteriocin capable of inhibiting the growth of pathogenic bacteria of P. stutzeri, A. hydrophila and V. alginolyticus. The best isolates obtained from results of this research was UG4 with clear zones ranging from 7.2 to 11.4 mm and the inhibition activity of 1476.53 mm²/mL after fragmentation with (NH4)2SO4 against pathogenic bacteria P. stutzeri was incubated at room temperature (37°C). This inhibition was caused by the presence of antimicrobial compounds that are produced by probiotic bacteria, namely bacteriocin. For isolates UG2 codes, the bacteria was resistant in which characterized by the formation of bluring zone around the paper disc.Key words: Antimicrobial activity, probiotics, bacteriocins, giant prawns
ANTAGONISM TEST OF PROBIOTICS BACTERIA ISOLATED FROM BLACK TIGER SHRIMP (Penaeus monodon) AGAINST PATHOGENS (Aeromonas hydrophila, Pseudomonas sp and Vibrio alginolyticus) Nursit Wijayanto; Feliatra '; Syahril Nedi
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractProbiotics is one effective strategy in an effort to cope with the disease by pathogenic bacteria. The study aims to determine the level of pathogenic bacteria were isolated from the candidate probiotic intestinal tiger shrimp against pathogenic bacteria A. hydrophila, Pseudomonas sp and V. alginolyticus. Expected candidate probiotics can be used to combat the disease in fish and shrimp farming. Antagonism using disc diffusion test. Four isolates of candidate probiotic bacteria more dominant round, milky white color and yellow, smooth or scalloped edge. Four candidate probiotic bacteria can inhibit pathogenic bacteria A. hydrophila, Pseudomonas sp and V. alginolyticus. The highest inhibition zone on the candidate probiotics P1 against A. hydrophila, Pseudomonas sp of 11.7 mm and 11.1 mm. The highest inhibition zone on V.alginolyticus the candidate probiotic bacteria P2 of 11.4 mm.Keywords: Probiotics, Tiger Shrimp, antagonists, A. hydrophila, Pseudomonas sp,V. alginolyticus.
Growth Of Heterotrophic Bacteria At Sea Water Polluted By ‘Surf’ Detergent in The Water of Kayu Ara Village Siak Regency Riau Province Dewan Pratama Novian; Irwan Effendi; Feliatra '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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The research was conducted from February 2016 to April 2017 and was aimed to determine the effect of adding different concentrations of detergent on the growth of bacterial populations in the ocean water column and to see the ratio of bacterial persisted capability in different lengths of dissection. This research used experimental method with Completely Randomized Design. Seawater samples were analyzed at Marine Microbiology Laboratory, Fisheries and Marine Faculty of Riau University. Based on the results of the study, from day 0 untill day 10 after given detergent with different concentrations, bacteria experienced a decreasing trend of growth, in day 15 untill day 20, the trend of bacterial growth contaminated by detergent with different concentration relatively increased to stagnant. Based on the result of this study the addition of different detergent concentration in each treatment affect the growth of heterotrophic bacteria population in the sea water, where the higher concentration of detergent added the lower the ability of bacteria to grow and the ability of heterotrophic bacteria to survive at different concentrations of detergent, it can be concluded that the ability of heterotrophic bacteria to grow from days 0 to 20 has decreased in each of its concentrations.   Keywords: heterotrophic bacteria, Surf detergent.
Isolation and Characterization of Heterotrophic Bacteria from Residential Area and Low Salinity Seawater in Purnama Village Dumai Riau Province Mayda Susana; Feliatra '; Iesje Lukistyowati
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Heterotrophs is a bacteria that use organic carbon and inorganic nitrogen as a food source. The research was conducted from December 2016 to January 2017. The aim at this study was to isolate, determine the ability of bacteria isolates as an antibacterial to pathogenic bacteria and determine the characteristics of heterotrophic bacteria isolated from residential area and low salinity seawater based on PCR method technique 16S rDNA sequences. Isolation and identification morphology, biochemical test and antibacterial tests were analyzed in Marine Microbiology Laboratorium of Riau University. DNA and PCR analysis process was conducted in Biology Laboratorium of Riau University. The sequencing DNA process was conducted in PT. Genetika Science Indonesia, West Jakarta. The result showed that the twenty three pure isolates bacteria and only seven isolates could be sequenced. Bacterial isolates 20M2, 20M4, 20M5, 20M11, 30M6, 30M7 and 30M8 able to inhibit the growth of pathogenic bacteria. Based on the BLAST system accessed via World Wide Web, the isolated bacteria were: Bacillus toyonensis strain ACOPR1ISOXg, Bacillus subtilis strain Y37, Enterobacter cloacae strain ENC-3, Clostridium acetobulylicum strain S512, Bacillus cereus strain 4PLGES, Bacillus cereus strain ML267 and Bacillus thuringensis strain C17.Keywords: heterotrophic bacteria, isolation, characterization, 16S rDNA
The Antagonists Test of Probiotic Bacteria Isolated from Black Tiger Shrimp (Penaeus monodon fabricus) Against Pathogens Pseudomonas sp, Aeromonas hidrophyla, Vibrio alginolyticus Ranap Junani Sari; Feliatra '; Dessy Yoswaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 1 (2015): Wisuda Februari Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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AbstractProbiotic bacteria are beneficial microbes that are beneficial to improve the microbialbalance in the digestive tract. The purpose of research to test the antimicrobial activity ofprobiotic bacteria in inhibiting the growth of pathogenic bacteria Pseudomonas sp,Aeromonas hidrophyla, and Vibrio algonolyticus. The method used in penilitian is a surveymethod, ie dengam observe and identify probiotic bacteria isolated from the gastrointestinaltract tiger shrimp (Penaeus monodon fabricus).The results showed that that the greatest inhibition zone strains of probiotic bacteriacontained in the code R10 is able to inhibit the activity of pathogenic bacteria P. stutzeri witha large zone of inhibition was 9.93 mm, the largest zone of inhibition of bacterial pathogensA. hydrophila is a probiotic bacterial strain with code R8 with a large zone of inhibition is9:46 mm, and most large inhibition zones of bacterial pathogens are present in V.alginolyticus strains of probiotic bacteria with the code R10 with a large zone of inhibitionwas 8.90 mm.Keywords : Probiotic Bacteri, Black Tiger Shrimp, Against Pathogens
VARIATION OF CONCENTRATION ON VIABILITY PROBIOTICS POWDER COATING EFFERVESCENT TABLETS Lora Lestari Lubis; Devi Ambarwaty Oktavia; Feliatra '
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 1 (2017): Wisuda Februari Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Efforts to increase shrimp production one through the use of probiotics. To maintain the viability of probiotic bacteria it is necessary need to protect bacteria by coating maltodextrin. Next will be molded into effervescent tablets to more intensive in the protection of probiotic bacteria. This study aimed to test the viability (the number of bacteria and soluble) probiotic that has been modified into effervescent tablets with variation in the concentrations of the coating. The method used is an experimental method in which samples of shrimp pond water will be increased 4 effervescent tablets (Formula I, II, III, IV) with 3 coating concentrations of 20%, 30%, and 40% maltodextrin. Each treatment be repeated two times. To determine the concentration of effervescent tablet coating formula is most effective test TPC (Total Plate Count) and the test of time to dissolve. Based on the test showed that the viability (number of bacteria and soluble time) probiotics in effervescent tablets with a coating of maltodextrin the best and the most effective in maintaining the stability of bacteria is of formula IV with a concentration of 30% by number of bacteria of 6.46 log CFU / mL and soluble within 20 menit. tatistical tests, the coating concentration factor (P) and the effervescent tablet formula (Q) has significant effect (p <0.05).Keywords: probiotics, effervescent tablets, Total Plate Count, viability
TEST OF BACTERIOCIN ANTIMICROBIAL ACTIVITY OF PROBIOTIC BACTERIA ISOLATED FROM TIGER SHRIMP (Penaeus monodon Fabricus) Anita R Sidabutar; Feliatra &#039;; Andi Dahliaty
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 2, No 2 (2015): Wisuda Oktober Tahun 2015
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Probiotic bacteria is a type of lactic acid bacteria (BAL) that produces compound of bacteriocin antimicrobial. Research to analyzed the bacteriocin of 5 probiotic bacterial from isolates tiger shrimp (Penaeus monodon Fabricus). The bacteriocin product contained in 2 forms, that i.e. without precipitation (extract bacteriocin) and with precipitation (fragment bacteriocin) used ammonium sulfate (NH4)2SO4. Bacteriocin will be tested activity against pathogen bacterial that cause fish disease was is Vibrio alginolyticus, Aeromonas hydrophila and Pseudomonas stutzeri. Inhibitory activity test conducted using paper disc diffusion method. Inhibitory activity bacteriocin with precipitation showed inhibition was higher the biggest than without precipitation. Fragment bacteriocin (BC) with precipitation had inhibitory activity or AU (Activity Unit) i.e. 881,71 mm2/ml at UWH9 isolate toward bacteria test A. hydrophila and P. stutzeri. The biggest Inhibitory activity of extract bacteriocin (CR) without precipitation was 365,65mm2/ml at UWH10 isolate toward bacteria test P. stutzeri.Keywords: Bacteriocin, Pathogen bacterial, Precipitation, Inhibitory activity
Using of Bacteria Heterotrophic as an Anti-Bacterial Againts Pathogenic Bacteria Isolated from Sea Water in Dumai City , Riau Province Dewi Sujani Adithiya; Feliatra &#039;; Afrizal Tanjung
Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan Vol 4, No 2 (2017): Wisuda Oktober Tahun 2017
Publisher : Jurnal Online Mahasiswa (JOM) Bidang Perikanan dan Ilmu Kelautan

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Heterotrophic bacteria have a role as decomposer of organic compounds (mineralization) and inorganic nitrogen (ammonia, nitrite, and nitrate) derived from industrial waste, decomposition of uneaten feed, faecal, excretion of fish and have the ability to inhibit the growth of pathogenic bacteria. This research was conducted in November untill December 2016. The results of antagonism test showed isolated bacteria have the ability to inhibit the growth of pathogenic bacteria (Vibrio algynolyticus, Aeromonas hydrophila and Pseudomonas sp.) Zone of inhibition in D5, D6 and D4 isolates classified in the strong category with zone of inhibition average of 10.5 to 11.8 mm and zone of inhibition in D1, D2, D3, D5, D7 isolates classified in the medium category with zone of inhibition average of 5.3 to 8.5 mm and each of the isolates are also classified in the weak category. The results of DNA analysis of heterotrophic bacteria isolated from marine waters of industrial area and low salinity of estuarine waters by using the PCR method technique 16S sequences found three strains of bacteria from the highest level of homology Bacillus toyonensis, Bacillus cereus and Bacillus pseudomycoides. D1 isolates has similarity to Bacillus cereus strain ASK16, D2 isolates to Bacillus toyonensis strain AC OPR1150Xg, D3 isolates to Bacillus pseudomycoides strain F1-8, D4 isolates to Bacillus cereus strain SBFW51, D5 isolates to Bacillus cereus strain OPP5 3-2, D6 to Bacillus cereus strain SBFW5S, D7 isolates to Bacillus cereus strain B4. Most of isolated bacteria obtained from the waters of industrial area because it receives much of nutrients that are very influential in the growth of bacteria.Kata Kunci : Bakteri Hetrotrofik, Antagonisme, Sekuens 16S rRNA