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All Journal Jurnal Ilmu Dasar Jurnal Matematika & Sains UNEJ e-Proceeding Jurnal Kimia Riset Molekul: Jurnal Ilmiah Kimia Jurnal Abdi: Media Pengabdian Kepada Masyarakat Bubungan Tinggi: Jurnal Pengabdian Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service)
Purkan Purkan
Departemen Kimia Fakultas Sains Dan Teknologi Universitas Airlangga
Humanities Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Decision Sciences, Operations Research & Management Education Environmental Science Languange, Linguistic, Communication & Media Mathematics Social Sciences Other

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Novel Mutations in the katG Gene in Isoniazid Resistant Mycobacterium tuberculosis Isolate Purkan Purkan; Ihsanawati Ihsanawati; Debbie Soefie Soefie; Yana Maolana Syah; Achmad Saifuddin Noer; Dessy Natalia
Jurnal Matematika & Sains Vol 18, No 1 (2013)
Publisher : Institut Teknologi Bandung

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Abstract

Isoniazid (INH) resistance in Mycobacterium tuberculosis is commonly associated with mutations in katG encoding catalase-peroxidase.  A clinical isolate of M. tuberculosis L21 exhibits isoniazid resistant phenotype.  A 2.2 kb DNA fragment of katG gene from the L21 isolate had been cloned and characterized.  The L21 katG gene has four point mutations, namely T898C; G1388T; T1997C; C2035T; and one G2203 deletion.  The in silico translation analysis of the katG mutant suggests that the mutation results in a truncated protein lacking of 62 C-terminal amino acids with three amino acid substitutions, Trp300Arg, Arg463Leu, and Ile666Thr. The structural model superposition of the mutant protein with the full length protein suggests that the mutant losses an ability to form a dimer structure, and also posseses a distortion of intermolecular interactions in the substrate binding channel. Keywords: katG gene, KatG, MDR M. tuberculosis, Isoniazid resistance, Structure model.   Beberapa Mutasi Baru pada Gen katG pada Isolat Mycobacterium tuberculosis Resisten Isoniazid Abstrak Resistensi Mycobacterium tuberculosis terhadap isoniazid umumnya berkaitan dengan mutasi yang terjadi pada gen katG yang pengode katalase-peroksidase.  Isolat klinis M. tuberculosis L21 fenotip resisten terhadap isoniazid.  Fragmen DNA berukuran 2,2 kb dari gen katG dari isolate L21 telah diisolasi dan dikarakterisasi. Gen katG dari isolat L21 memiliki empat mutasi, yaitu T898C; G1388T; T1997C; C2035T; dan delesi G2203.  Analisis in silico terhadap gen katG mutan menunjukkan bahwa mutasi yang terjadi menhasilkan protein mutan yang kehilangan 62 asam amino pada ujung-C yang juga mengandung perubahan asam amino Trp300Arg, Arg463Leu, dan Ile666Thr. Superposisi model struktur protein antara protein mutan dengan protein utuh menyarankan bahwa protein mutan diduga tidak dapat membentuk struktur dimer dan juga mengandung interaksi molekular pada poket pengikatan substrat yang berbeda. Kata kunci: Gen katG, M. tuberculosis, Resisten isoniazid, Model struktur.
Unique Cellulase Enzyme of Bacillus firmus From Organic Waste ., Purkan; ., Sumarsih; A, Rachmadani D
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Abstract

The objectives of this research are to isolate cellulolytic bacteria from liquid fermentation of organic waste, to determine the optimum conditions of cellulose enzyme production and characterize of crude extract cellulase enzyme activity, including pH and optimum temperature. Cellulolytic bacteria was isolated with pour plate method using specific media CMC (Carboxymethyl Celullose) and incubated at  the temperature of 370C for 24 hours. The activity of cellulolytic bacteria can be seen by the presence of transparent zone around the place where colony grows. The identification of isolates was done macroscopically, microscopically and biochemistry. Cellulolytic enzyme activity was determined with Miller method to CMC substrate. In this research, 2 isolates of cellulose producer bacteria with the biggest cellulolytic index of 1,903 (DA1) was obtained. Optimum condition of cellulose enzyme production by the bacteria was at hour-24 with the optimum molasses concentration of 0,4% and producing enzyme activity which is 0,04802 U/mL. The cellulose enzyme activity which was produced by Bacillus firmus isolate showed that the crude enzyme has  optimum activity at  pH 5 and temperature of 500C. Keywords: organic waste, Bacillus firmus,  cellulolytic bacteria, cellulose
Unique Cellulase Enzyme of Bacillus firmus From Organic Waste Purkan .; Sumarsih .; Rachmadani D A
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UPT Penerbitan Universitas Jember

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Abstract

The objectives of this research are to isolate cellulolytic bacteria from liquid fermentation of organic waste, to determine the optimum conditions of cellulose enzyme production and characterize of crude extract cellulase enzyme activity, including pH and optimum temperature. Cellulolytic bacteria was isolated with pour plate method using specific media CMC (Carboxymethyl Celullose) and incubated at  the temperature of 370C for 24 hours. The activity of cellulolytic bacteria can be seen by the presence of transparent zone around the place where colony grows. The identification of isolates was done macroscopically, microscopically and biochemistry. Cellulolytic enzyme activity was determined with Miller method to CMC substrate. In this research, 2 isolates of cellulose producer bacteria with the biggest cellulolytic index of 1,903 (DA1) was obtained. Optimum condition of cellulose enzyme production by the bacteria was at hour-24 with the optimum molasses concentration of 0,4% and producing enzyme activity which is 0,04802 U/mL. The cellulose enzyme activity which was produced by Bacillus firmus isolate showed that the crude enzyme has  optimum activity at  pH 5 and temperature of 500C. Keywords: organic waste, Bacillus firmus,  cellulolytic bacteria, cellulose
PRODUKSI ENZIM KITINASE DARI Aspergillus niger MENGGUNAKAN LIMBAH CANGKANG RAJUNGAN SEBAGAI INDUSER Purkan Purkan; Afaf Baktir; Arju Rohmah Sayyidah
Jurnal Kimia Riset Vol. 1 No. 1 (2016): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.796 KB) | DOI: 10.20473/jkr.v1i1.2440

Abstract

AbstrakKitinase merupakan enzim hidrolitik yang dapat menghidrolisis kitin pada ikatan β-1,4-glikosidiknya dengan menghasilkan derivat-derivat kitin seperti oligomer kitin yang mempunyai banyak manfaat. Penelitian ini bertujuan untuk melakukan pengembangan produksi enzim kitinase dari sumber lokal yang melimpah di alamserta murah dengan melakukan optimasi substrat dalam hal ini digunakan substrat tetes tebu (molase) dan limbah cangkang rajungan untuk produksi enzim kitinase dari Aspergillus niger. Sebelumnya, dilakukan kultivasi isolat kapang Aspergillus niger dengan membuat kurva pertumbuhan menggunakan metode masa sel kering dimana dari hasil penelitian inokulasi optimal adalah 22 jam. Pada proses produksi, diperoleh waktu fermentasi optimal adalah 52 jam dengan menentukan uji aktivitasnya menggunakan metode turbidimetri. Hasil optimasi substrat menunjukkan bahwa enzim kitinase yang maksimal diperoleh pada penambahan molase 0,5% (b/v) dengan unit aktivitas enzim 0,14726 (U/mL) dan cangkang rajungan 2% (b/v) dengan unit aktivitas enzim yang dihasilkan 0,12826 (U/mL). Kitinase dari Aspergillus niger ini mempunyai pH optimal 6 dan suhu optimal 40 oC. Kata kunci: Aspergillus niger, kitinase, cangkang rajungan, molase   AbstractChitinase is a hydrolytic enzyme that hydrolyzes chitin on β-1,4-glycosidic bond and thereby producing chitin derivatives such as chitin oligomers that have multiple benefits. The purpose of this research was to develop the production of chitinase enzyme from cheap and are abundant local nature sources, by optimizations substrate in this case the substrate used molasses and crab shell waste for the production of chitinase enzyme from Aspergillus niger. Previously, isolates of Aspergillus niger cultivated by creating a growth curve using dry cell mass method which from the results of research inoculation optimal are 22 hours. In the production process, obtained the optimum fermentation time is 52 hours to determine the activity test using turbidimetry method. Result of substrate optimizations indicate that chitinase enzyme maximum by addition of molasses obtained in 0.5% (w/v) with enzyme activity units 0.14726 (U/mL) and crab shells 2% (w/v) with enzyme activity units 0.12826 (U/mL). Chitinase from Aspergillus niger has a pH optimum 6 and temperature optimum 40 oC. Keywords: Aspergillus niger, chitinase, crab shells, molasses
Lactobacillus bulgaricus Sebagai Probiotik Guna Peningkatan Kualitas Ampas Tahu Untuk Pakan Cacing Tanah Purkan Purkan; Nur Nisdiyatul Laila; Sri Sumarsih
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (791.751 KB) | DOI: 10.20473/jkr.v2i1.3688

Abstract

AbstrakPenelitian ini bertujuan untuk menentukan aktivitas protease dari probiotik Lactobacillus bulgaricus dan pengaruh probiotik Lactobacillus bulgaricus dalam fermentasi pakan ampas tahu untuk meningkatkan produktivitas cacing tanah. Metode yang digunakan untuk penentuan aktivitas protease dalam hidrolisis substrat kasein adalah metode Bradford. Dari hasil penelitian, probiotik Lactobacillus bulgaricus mengeluarkan protease selama 18 jam pertumbuhan, dengan aktivitas protease sebesar 131,04 U/mL. Probiotik Lactobacillus bulgaricus OD 0,6 dapat menghidrolisis protein ampas tahu sebesar 1,48 µg/mL dalam 12 jam fermentasi. Produktivitas cacing tanah mengalami peningkatan berat cacing tanah karena adanya pengaruh probiotik Lactobacillus bulgaricus pada pakan ampas tahu yang ditunjukkan dengan persen kenaikan berat cacing tanah sebesar 32,13%.Kata kunci: Probiotik, Lactobacillus bulgaricus, Enzim protease, Cacing tanah
Production of Cellulase Enzyme from Aspergilus niger using Rice Husk and Bagasse as Inducer Purkan Purkan; HD Purnama; S Sumarsih
Jurnal ILMU DASAR Vol 16 No 2 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.132 KB) | DOI: 10.19184/jid.v16i2.2768

Abstract

Aspergillus niger is fungi can produce cellulase enzyme with agriculture waste as natural inducers. The purpose of this study was to compare the natural inducers potential between rice husk and bagasse to produce cellulase enzyme from Aspergillus niger. Production of cellulase enzyme was done with variety of inducers such as CMC, rice husk, and bagasse. The optimization of enzyme production includes optimum production time, inducer type, and optimum concentration of inducer. Furthermore, the enzyme also was characterized in pH and temperature. Enzyme activity test using the DNS method with CMC as substrate. According of this test result show that highest cellulase enzyme activity has production time for 108 hours with rice husk as inducer. The optimum rice husk concentration was needed of 2.5%. The cellulase enzyme was induced by rice husk has optimum activity at pH 4 and 50°C of 0.709 IU/mL.   Keywords : cellulase enzymes, Aspergillus niger, inducers, rice husk, bagasse.
RESISTANCE LEVEL OF Pseudomonas stutzeri AGAINST MERCURY AND ITS ABILITY IN PRODUCTION OF MERCURY REDUCTASE ENZYME Purkan Purkan; Safita Nurmalyya; Sofijan Hadi
Molekul Vol 11, No 2 (2016)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (511.85 KB) | DOI: 10.20884/1.jm.2016.11.2.256

Abstract

Mercury reductase is an enzyme that is able to reduce Hg2+ to Hg0 non toxic. This enzyme is usually produced by mercury resistant bacteria. The research wanted to determine the resistance of indigenous Pseudomonas stutzeri isolate toward mercury and to explore the mercury reductase activity which is produced by the bacteria. The results of resistance assay of the Pseudomonas stutzeri toward mercury ion showed that the isolate could survive in media containing HgCl2 up to a concentration of 80 µM. The bacteria could produce mercury reductase optimally at the 24th of fermentation time. The enzyme showed optimum activity at pH 7 and temperature of 45 oC
Biochemical Properties of Mercuric Reductase from Local Isolate of Bacillus sp for Bioremediation Agent Purkan Purkan; Yuliana Firdausi Nuzulla; Sofijan Hadi; Endang Triwahyu Prasetyawati
Molekul Vol 12, No 2 (2017)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (357.854 KB) | DOI: 10.20884/1.jm.2017.12.2.398

Abstract

Mercuric reductase is the important enzyme which catalyzes a reduction of a toxic Hg2+ to non-toxic Hg0. The enzyme which has been potentially used as mercury bioremediation agent is produced by mercury resistant bacteria. These research aims are to determinate the resistance level of a local Bacillus sp to HgCl2 in media, to determine the mercuric reductase activity from the bacteria, and to determine the biochemical properties of the mercuric reductase. The Bacillus sp was grown in the Nutrient Broth media with various of  0; 20; 40; 60; 120; and 160 µM HgCl2 to know the response of the bacteria against mercury, The cell growth of Bacillus sp was measured by optical density (OD) method of at λ 600 nm. The mercuric reductase activity was assayed in the solution of MRA (Mercury Reductase Assay), then the oxidized NADPH was observed by the spectrophotometry method at λ340 nm. The result showed that the Bacillus sp has been resistant to media containing mercury at 120 µM, but the microbial growth was decreased by 50% in media containing mercury 80 µM. The Bacillus sp could produce highly the mercuric reductase enzyme at 16 hours of growth time with enzyme activity as 0.574 Unit/µg. The mercuric reductase from the bacteria has an  optimum activity at pH 6 and temperature 37 °C
EKSPLORASI BAKTERI KITINOLITIK DARI SAMPAH ORGANIK : ISOLASI DAN KARAKTRISASI ENZIM KITINASE Purkan Purkan; Badi’atul Azizah; Afaf Baktir; Sri Sumarsih
Molekul Vol 9, No 2 (2014)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.612 KB) | DOI: 10.20884/1.jm.2014.9.2.160

Abstract

Enzim kitinase banyak digunakan dalam bidang medis, makanan, bioteknologi dan lingkungan. Banyaknya kebutuhan enzim kitinase menuntut penyediannya yang murah dan melimpah dengan teknologi produksi yang sederhana. Penelitian ini bertujuan untuk isolasi mikroba kitinolitik dari cairan fermentasi sampah organik, produksi dan uji aktivitas enzim kitinase serta mengetahui karakteristik dari enzim kitinase. Isolasi mikroba telah dilakukan dengan  metode spread plate. Aktivitas kitinase ditentukan secara kualitatif dengan pengukuran indeks kitinolitik dan secara kuantitatif dengan pengukuran absorbansi menggunakan spektrofotometer Uv-Vis pada panjang gelombang 660 nm berdasarkan banyaknya substrat kitin yang dihidrolisis oleh enzim kitinase. Satu dari beberapa isolat yang didapatkan, yaitu isolat A1 menunjukkan aktivitas kitinolitik tertinggi, yaitu sebesar 1,21. Hasil identifikasi mikrobiologi menunjukkan bahwa isolat A1dinyatakan sebagai Pseudomonas pseudomallei. Bakteri ini mampu menghasilkan kitinase secara optimum pada jam ke 18 waktu fermentasi, dengan penambahan molase 0,5% (b/v) dan 1% kitin (b/v) pada media produksinya. Kitinase yang dihasilkan P.  pseudomallei menunjukkan aktivitas optimum pada suhu 50 °C danpH sebesar 6.
Pelatihan Pembuatan Hand Sanitizer Menuju Desa Cerdas Kesehatan Di Desa Cangkir, Kecamatan Driyorejo, Kabupaten Gresik Qurrota A'yuni; Alfa Akustia Widati; Harsasi Setyawati; Atik Widiyanti; Purkan Purkan; Miratul Khasanah; Tokok Ardiarto; A. Budi Prasetyo; Aning Purwaningsih; Siti Wafiroh; Sri Sumarsih; Rico Ramadhan; Sofijan Hadi; Kariza Makanty; Ahlan Riwahyu Habibi; Aulianitha Salsabella
Bubungan Tinggi: Jurnal Pengabdian Masyarakat Vol 4, No 2 (2022)
Publisher : Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/btjpm.v4i2.5253

Abstract

Hand sanitizer merupakan cairan atau gel pembersih tangan yang memiliki sifat sebagai antibakteri dalam menghambat hingga membunuh bakteri. Dengan demikian hand sanitizer lebih efektif dalam membasmi kuman. Berdasarkan penelitian The Centers for Disease Control and Prevention (CDC), hand sanitizer dengan kandungan alkohol minimal 60% dapat berfungsi sebagai antibakteri maupun antivirus. Hand sanitizer pernah menjadi barang langka di puncak pandemi pada tahun 2020, karena pembelian yang masif dari masyarakat Indonesia. Langkanya hand sanitizer di beberapa toko dan apotek membuat harganya menjadi mahal. Kegiatan Pengabdian Kepada Masyarakat ini bertujuan untuk meningkatkan pengetahuan akan pentingnya kesehatan dan kemungkinan penyebaran kuman terutama virus dan bakteri serta upaya untuk mengantisipasinya. Tujuan yang lain yaitu untuk meningkatkan keterampilan masyarakat dalam pembuatan hand sanitizer. Rangkaian kegiatan ini diselenggarakan di Desa Cangkir, Kecamatan Driyorejo, Kabupaten Gresik pada Bulan November-Desember 2021 secara luring dengan menerapkan protokol kesehatan dengan peserta Ibu-Ibu PKK dan Kader PKK. Kegiatan ini dilakukan melalui metode pendampingan dan demonstrasi dengan presentasi materi dan praktik langsung. Kegiatan ini memberikan dampak yang positif bagi masyarakat Desa Cangkir dalam menghadapi Pandemi Covid-19 saat ini. Hand sanitizer is a liquid or gel that has antibacterial properties to inhibit and kill bacteria. Thus, hand sanitizer is more effective in eradicating germs. Based on research from The Centers for Disease Control and Prevention (CDC), alcohol-based hand sanitizers that contain at least 60% alcohol can function as both antibacterial and antivirus. Hand sanitizer was once a scarce item at the height of the pandemic in 2020, due to massive purchases from the Indonesian people. The scarcity of hand sanitizers in some shops and pharmacies makes the price expensive. This Community Service activity aims to increase knowledge of the importance of health and the possibility of spreading germs, especially viruses and bacteria, and efforts to anticipate them. Another aim is to improve community skills in making hand sanitizers. This series of activities were held in Cangkir Village, Driyorejo District, Gresik Regency in November-December 2021 via offline by implementing health protocols with PKK women and PKK Cadres as participants. This activity is carried out through mentoring and demonstration methods with presentations and hands-on practice. This activity has a positive impact on the Cangkir Village community in dealing with the current Covid-19 Pandemic. 
Co-Authors A. Budi Prasetyo Achmad Saifuddin Noer AFAF BAKTIR Ahlan Riwahyu Habibi Alfa Akustia Widati Aning Purwaningsih Arju Rohmah Sayyidah Atik Widiyanti Aulianitha Salsabella Badi’atul Azizah Debbie Soefie Soefie Dessy Natalia Diana Merna Eksan Ar Rasyid Fatiha Khairunnisa Fatiha Khairunnisa Hamizah Haula' Harsasi Setyawati Hartati Hartati HD Purnama Ihsanawati Ihsanawati Kariza Makanty Kautsar Ul Haq Miratul Khasanah Muji Harsini Mulyadi Tanjung Nur Nisdiyatul Laila Pratiwi Pudjiastuti Qurrota A’yuni Rachmadani D A Rachmadani D A, Rachmadani D Rico Ramadhan S Sumarsih Safita Nurmalyya Siti Wafiroh Sofijan Hadi Sri Sumarsih Sri Sumarsih Sri Sumarsih Sumarsih . Sumarsih . Tokok Ardiarto Yana Maolana Syah Yanuardi Raharjo Yuliana Firdausi Nuzulla