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All Journal IPTEK Journal of Proceedings Series Jurnal Matematika dan Ilmu Pengetahuan Alam Indonesian Journal of Tropical and Infectious Disease INDONESIAN JOURNAL OF PHARMACY Jurnal Kimia Riset Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya Jurnal Abdi: Media Pengabdian Kepada Masyarakat Bubungan Tinggi: Jurnal Pengabdian Masyarakat
Harsasi Setyawati
Departemen Of Chemistry, Faculty Of Science Technology, Universitas Airlangga, Indonesia
Humanities Chemistry Computer Science & IT Earth & Planetary Sciences Education Health Professions Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Penentuan Kondisi Optimum Penyerapan Logam Kadmium Oleh Asam Humat Darmokoesoemo, Handoko; Setyawati, Harsasi
Jurnal Matematika dan Ilmu Pengetahuan Alam Vol 17, No 1 (2015)
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (50.452 KB)

Abstract

This research was study on the formation of complexes between metals Cd(II) with humic acid and the optimum conditions of metal Cd(II) adsorption by humic acid. Determination of optimization conditions of metal Cd(II) adsorption by humic acid i.e. variation of contact time, pH and Cd(II) concentration. The results showed the optimum contact time was 90 minutes with a percentage of Cd adsorped was 86.52%, the pH optimum was pH 6 with a percentage of Cd adsorped was 80.39% and the optimum Cd(II) concentration was 500 ppm with the optimum Cd(II) adsorped by humic acid was 221, 8 mg /L. FTIR analysis showed the presence a bond between the metal Cd(II) with oxygen groups of humic acid at wavelength of 354.90 cm-1. The adsorption model of Cd(II) by humic acids showed Langmuir type with correlation coefficient 0,99909.Keywords : humic acid, cadmium, Cd(II)-humat complexes.
Toksisitas Senyawa Tembaga(II)Klorida Dihidrat Terhadap Sel Kanker Payudara T74D Secara in vitro Teguh Hari Sucipto; Harsasi Setyawati; Fahimah Martak
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 2, No 2 (2018)
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.624 KB) | DOI: 10.30651/jqm.v2i2.1433

Abstract

Cancer is a transformation of normal cells in the body into malignancy due to the induction of carcinogens. Breast cancer is the second leading cause of death in Indonesia. The field of treatment with inorganic compounds has been widely developed and shows better anticancer activity than organic compounds. This research aims to know the toxicity level of copper value of 29.021 μg/ml. The value of IC 50 obtained <100 μg/ml so that the copperCancer is a transformation of normal cells in the body into malignancy due to the induction ofcarcinogens. Breast cancer is the second leading cause of death in Indonesia. The field oftreatment with inorganic compounds has been widely developed and shows better anticanceractivity than organic compounds. This research aims to know the toxicity level ofcoppervalue of 29.021 μg/ml. The value of IC50obtained<100 μg/ml so that the copper
Glycerol Acetylation Using Magnesium Fluoride Catalyst to Produced DAG and TAG Qurrota A’yuni; Syafsir Akhlus; Harsasi Setyawati; Irmina Kris Murwani
IPTEK Journal of Proceedings Series No 4 (2017): 2nd International Seminar on Chemistry 2016
Publisher : Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (734.884 KB) | DOI: 10.12962/j23546026.y2017i4.3076

Abstract

Glycerol acetylation with acetic acid using magnesium fluoride catalyst have been investigated. Magnesium fluoride catalyst was prepared by sol-gel method with ethanol as gelating agent. The glycerol acetylation produced monoacetylglyceride (MAG), diacetylglyceride (DAG) and triacetylglyceride (TAG). The catalytic reaction carried out by thermal methods reflux under ambient conditions. The influence of reaction conditions such as the reaction time on the product distribution was investigated. The optimum of glycerol conversion was achieved at 5 hours of reaction time by 78.34% with selectivity of 30, 62 and 8% for MAG, DAG and TAG respectively. The reaction route over magnesium fluorides was observed by product as reactants, gradually. The results showed that the acetylation reaction of glycerol occurs via a parallel route to the first, followed by the consecutive route to produce DAG and TAG.
SINTESIS DAN KARAKTERISASI SENYAWA KOMPLEKS Zn(II)-EDTA SEBAGAI SENAYAWA ANTIALGA PADA COOLING WATER INDUSTRI Harsasi Setyawati; Sri Sumarsih; Sevia Ayuningtyas
Jurnal Kimia Riset Vol. 2 No. 1 (2017): Juni
Publisher : Universitas Airlangga, Campus C Mulyorejo, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1030.612 KB) | DOI: 10.20473/jkr.v2i1.3689

Abstract

ABSTRACTA research on the synthesis and characterization of complex compounds of Zn (II)-EDTA as antialgae compound is applied to the cooling water industry. This research aims to determine the activity of complex compounds of Zn (II)-EDTA against algae that live in the water cooling water. The activity antialgae assay of comple compound of Zn(II)-EDTA with luminescence method and dry cell weight method. Complex compound of Zn (II)-EDTA made with mole ratio of ZnCl2: Na2EDTA is 1:1. Complex compound of Zn (II)-EDTA analyzed using UV-Vis spectrophotometer and FTIR spectrophotometer. The results of UV-Vis spectrophotometer analysis showed that the complex compounds of Zn (II)-EDTA has a maximum wavelength at 752 nm. While the results of FTIR analysis showed  Zn-O vibration absorption at wave number 478.35 cm-1 and Zn-N vibration absorption at wave number 516.92 cm-1. In the activity antialgae assay of complex compound of Zn (II)-EDTA made with a concentration of 5 ppm, 10 ppm, 50 ppm and 100 ppm. The test results showed that the activity of complex compounds of Zn (II) -EDTA can kill green algae and brown algae. Of the four concentrations of complex compounds of Zn (II)-EDTA, green algae and brown algae can be killed optimally at a concentration of 50 ppm.Keywords: complex Zn(II)-EDTA, cooling water, antialgae, green algae, brown                   algae 
The Influence of pH Values on the Crystallite Size of ZnO by Solvothermal Synthesis Ahmadi Jaya Permana; Dian Wulandari; Hartati Hartati; Harsasi Setyawati; Mochammad Zakki Fahmi
Jurnal Kimia Riset Vol. 2 No. 2 (2017): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (222.782 KB) | DOI: 10.20473/jkr.v2i2.6430

Abstract

 Zinc oxide (ZnO) is a semiconductor material that widely used in various applications due to its unique properties. Synthesis of ZnO by solvothermal method has been conducted with controlled pH values. The variations of pH value were 10, 11 and 12 by adjusting NaOH content. Crystall structure of the synthesis products after heat treatment at 110oC and 600oC has characterised by X-ray Diffratometer (XRD). Crystallite size of ZnO was measured by Scherrer equation. Crystall phase of ZnO has been observed on all pH value variations at 110 oC with 22,98-37,06 nm of crystallite size, whereas ZnO has been observed on all pH value variations at 600 oC with 41,39-71,77 nm of crystallite size.Keywords: ZnO, pH values, crystallite size, solvothermal
RNA Isolation of Dengue Virus Type 2 with Different Precipitation Solvents : Methanol, Chloroform, and 2-Isopropanol. Yovilianda Maulitiva Untoro; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Shuhai Ueda; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 1 (2018): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (416.739 KB) | DOI: 10.20473/jkr.v3i1.7455

Abstract

Dengue virus distributed in tropical and subtropical regions in the world. DENV viruses are transmitted between humans primarily by Aedes aegypti and Aedes albopictus mosquitoes and are endemic in most areas in which the vectors occur. Four serotypes of dengue virus are DENV-1, DENV-2, DENV-3 and DENV-4. DENV-2 is comprised of six genotypes. Ethanol precipitation is a commonly used technique for concentrating and de-salting nucleic acids (DNA or RNA) preparations in aqueous solution. RNA isolation by combining Guanidinium thiocyanate and phenol reported has been reported. In this report, we investigated RNA isolation from DENV-2 using QIAamp Mini Kit with 2-Isopropanol, Methanol, Chloroform precipitation solvent. Electrophoregram showed DNA band as  the result of RNA isolation with methanol and 2-isopropanol are produced quite well. Dna band of the of RNA isolation with chloroform solvent has the lowest intensity than methanol and 2-isopropanol. This study showed that methanol and 2-isopropanol  can used as precipitation solvent for isolating RNA.
Precipitation Solvents for RNA Extraction of Dengue Virus Type 3: Dimethylformamide, Ethylenediamintetraacetic Acid, and Ultrapure H2O Rizqidhana Juliana Putri; Teguh Hari Sucipto; Harsasi Setyawati; Siti Churrotin; Ilham Harlan Amarullah; Puspa Wardhani; Aryati Aryati; Soegeng Soegijanto
Jurnal Kimia Riset Vol. 3 No. 2 (2018): Desember
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (477.838 KB) | DOI: 10.20473/jkr.v3i2.9353

Abstract

Dengue is a disease caused by a virus from the family Flaviviradae, carried by a female mosquito of Aedes aegypti species. Dengue fever is widespread in the tropic areas. It caused by rainfall, temperature and unplanned urbanization. According to the ministry of health , almost all provinces in Indonesia are endemic areas of dengue fever. In 2014, up to mid-December Dengue Hemorrhagic Fever (DHF) patients in 34 provinces in Indonesia are 71,668 people and 641. This figure is lower than the previous year, 2013 with 112,511 people and 871 deaths . This disease consists of four types of serotypes, namely DENV-1, DENV-2, DENV-3, and DENV-4. This disease can be identified using a variety of methods, one of the method is Reverse Transcription - Polymerase Chain Reaction (RT-PCR) method. This study aims to determine the ability of Dimethylformamide (DMF), Ethylenediamintetraacetic Acid (EDTA), and Ultrapure H2O as the substitute of  Ethanol for precipitation in RNA extraction process. The sample used in this research obtained from Surabaya. RNA extraction itself can be done by using a special kit for RNA extraction. In Reverse Transcription - Polymerase Chain Reaction method, first RNA is extracted and then transcribed back (Reverse Transcription) which then form cDNA that later will be amplified by using PCR method. In this study used specific primers for dengue virus type 3 (DENV-3). The results of this study show that DMF, EDTA, and Ultrapure H2O can be used as the substitute of Ethanol for precipitation on RNA extraction. The result is evidenced by the formation of viral DNA bands on gel electrophoresis results.
SINTESIS, KARAKTERISASI DAN UJI AKTIVITAS SENYAWA KOMPLEKS Zn(II)-KATEKIN SEBAGAI INHIBITOR ENZIM LIPASE Antyka Lutfiana Putri; Sri Sumarsih; Harsasi Setyawati
Jurnal Kimia Riset Vol. 4 No. 1 (2019): Juni
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (295.2 KB) | DOI: 10.20473/jkr.v4i1.13154

Abstract

ABSTRACT The study aims to synthesis, characterization and activity testing of Zn(II)-catechin as lipase inhibitor activity. The complex compound from synthesis result is characterized by its maximum wavelength, functional groups, metals and ligands bonds, and it’s melting point. This complex compound tested of Zn(II)-catechin as lipase inhibitor activity to p-NPP (para-nitrofenilpalmitat) substrate. The result shows that Zn(II)-catechin could be synthesized from Zn(II) ligans and metals with ratio ligands and metals mol is 1:1, that has maximum wavelength 454 nm, which contain Zn-O bond at 354,90 and 478,35 cm-1, and melting point of Zn(II)-catechin >250 oC. Zn(II)-catechin can inhibition 60,63% from concentration 50 µg/mL with mixed inhibition Keyword : Zn(II)-catechin, lipase, mixed inhibition ABSTRAK Penelitian ini bertujuan untuk melakukan sintesis, karakterisasi dan uji aktivitas senyawa kompleks Zn(II)-katekin terhadap aktivitas inhibitor enzim lipase. Senyawa kompleks hasil sintesis dikarakterisasi panjang gelombang maksimumnya, gugus fungsi serta ikatan logam dan ligannya, serta titik lelehnya. Senyawa kompleks diuji aktivitas inhibitor enzim lipase terhadap substrat p-NPP (paranitrofenilpalmitat). Hasil penelitian menunjukkan bahwa senyawa kompleks Zn(II)-katekin dapat disintesis dari ligan katekin dan logam Zn(II) dengan perbandingan mol ligan logam 1:1, mempunyai panjang gelombang maksimum 454 nm, mengandung ikatan Zn-O pada 354,90 dan 478,35 cm-1 dan titik leleh senyawa kompleks >250 oC. Senyawa kompleks Zn(II)-katekin pada konsentrasi 50µg/mL mempunyai daya inhinbisi sebesar 60,63% terhadap enzim lipase dengan jenis inhibisi campuran. Kata kunci : Zn(II)-katekin, lipase, inhibitor campuran
BETLE LEAF ESSENTIAL OIL FOR HEMOPHILIAC PATIENTS AND ITS ANTIBACTERIAL EFFECTS ON MYCOBACTERIUM TUBERCULOSIS Teguh Hari Sucipto; Nourmalasari Aisyah; Puji Lestari; Harsasi Setyawati
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 3 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.65 KB) | DOI: 10.20473/ijtid.v6i3.1387

Abstract

Betle leaf (Piper betle L.) is a medicinal plant. It contains essential oil and shows various biological activities, such as antibacterial, anticoagulant, etc. It is further reported to have low anticoagulant activities; thus, it is highly potential as a candidate for coagulant drug. Coagulant is used to prevent bleeding for patients with blood clotting disorders like hemophilia. In Indonesia, 1,236 people were reported with hemophilia. The standard parameters of anticoagulant activity are the freezing period and the compound concentrations. The purpose of this study was to determine the effect of betle leaf’s essential oil on blood coagulation in patients with factor VIII and IX of blood plasma disorders. The isolation of essential oil is conducted through steam distillation method with two kinds of solvents, namely distilled water and n-hexane. The obtained n-hexane extract is then separated from the liquid-liquid extraction and rotary evaporator. Essential oil is diluted with citrate plasma solution. The test results of blood clots increase as the concentration of essential oils increase. The results are recorded as such: essential oils ½ times dilution of 99.67 seconds; ¼ times dilution of 127 seconds; 1/8 times dilution of 179 seconds; and 1/16 times dilution of 242.67 seconds. The test above proves that the piper betle extract possesses a coagulant activity. The ethanol extract contained in the piper betle could stimulate clotting in the blood cells. It is caused by the increase of blood plasma concentration which further escalate the plasma fluid into the blood cells. Based on this study, the activity of Mycobacterium tuberculosis can be obstructed by betle leaf in ½ times dilution. The extract significantly reduces acid which accelerates bacteria development.
ANTIVIRAL ACTIVITY OF COPPER(II)CHLORIDE DIHYDRATE AGAINST DENGUE VIRUS TYPE-2 IN VERO CELL Teguh Hari Sucipto; Siti Churrotin; Harsasi Setyawati; Tomohiro Kotaki; Fahimah Martak; Soegeng Soegijanto
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 4 (2017)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (144.829 KB) | DOI: 10.20473/ijtid.v6i4.3806

Abstract

Infection of dengue virus (DENV) was number of globally significant emerging pathogen. Antiviral dengue therapies ar importantly needed to control emerging dengue. Dengue virus (DENV) is mosquito-borne arboviruses responsible for causing acute systemic diseases and grievous health conditions in humans. To date, there is no clinically approved dengue vaccine or antiviral for humans, even though there have been great efforts towards this end. Copper and copper compounds have more effective in inactivation viruses, likes an influenza virus and human immunodeficiency virus (HIV). Purpose in this project was investigated of Copper(II)chloride Dihydrate antiviral compound were further tested for inhibitory effect on the replication of DENV-2 in cell culture. DENV replication was measures by Enzyme linked Immunosorbent Assay (ELISA) with selectivity index value (SI) was determined as the ratio of cytotoxic concentration 50 (CC50) to inhibitory concentration 50 (IC50) for compound. The maximal inhibitory concentration (IC50) of Copper(II)chloride Dihydrate against dengue virus type-2 was 0.13 μg/ml. The cytotoxic concentration (CC50) of compound against Vero cell was 5.03 μg/ml. The SI values for Copper(II)chloride Dihydrate 38.69. Result of this study suggest that Copper(II)chloride Dihydrate demonstated significant anti-DENV-2 inhibitory activities and not toxic in the Vero cells. Copper mechanisms play an important role in the prevention of copper toxicity, exposure to excessive levels of copper can result in a number of adverse health effects, as a result increased reactive oxygen species and oxidative damage to lipid, DNA, and proteins have been observed in human cell culture models or clinical syndromes of severe copper deficiency and inhibition was attributed to released cupric ions which react with cysteine residues on the surface of the protease.
Co-Authors A. Budi Prasetyo Ahlan Riwahyu Habibi Ahmadi Jaya Permana Ahmadi Jaya Permana Alfa Akustia Widati Aning Purwaningsih Anisa Maharani Anisa Maharani Antyka Lutfiana Putri Aryati Aryati Aryati Aryati Aryati Aryati Aswandi Wibrianto Atik Widiyanti Aulianitha Salsabella DIAN WULANDARI Fahimah Martak Fatiha Khairunnisa Handoko Darmokoesoemo Handoko Darmokoesoemo Hartati Hartati Hartati Hartati Ilham Harlan Amarullah Ilham Harlan Amarullah Ilham Harlan Amarullah Ilham Harlan Amarullah Ilham Harlan Amarullah Kariza Makanty Kautsar Ul Haq Kris Cahyo Mulyatno Kris Murwani, Irmina Masanori Kameoka Miratul Khasanah Mochammad Zakki Fahmi Mulyadi Tanjung Nourmalasari Aisyah Novia Faridatus Sholihah Puji Lestari Purkan Purkan Puspa Wardhani Puspa Wardhani Puspa Wardhani Puspa Wardhani Qurrota A’yuni Rico Ramadhan Rizqidhana Juliana Putri Sevia Ayuningtyas Shuhai Ueda Shuhai Ueda Shuhai Ueda Siti Churrotin Siti Churrotin Siti Churrotin Siti Churrotin Siti Churrotin Siti Churrotin Siti Wafiroh Soegeng Soegijanto Sofijan Hadi Sri Subekti Sri Sumarsih Syafsir Akhlus Teguh Hari Sucipto Teguh Hari Sucipto Teguh Hari Sucipto Teguh Hari Sucipto Teguh Hari Sucipto Teguh Hari Sucipto Tokok Ardiarto Tomohiro Kotaki Tomohiro Kotaki Yanuardi Raharjo Yovilianda Maulitiva Untoro