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A.actinomycetemcomitans adhesi protein increasing IL-8 titre in heart of wistar rat with aggressive periodontitis (Protein adhesin dari A.actinomycetemcomitans meningkatkantiter IL -8 di dalamjantungtikuswistar denganperiodontitis agresif) Rini Devijanti Ridwan; Retno Indrawati
Journal of Dentomaxillofacial Science Vol. 13 No. 2 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i2.392

Abstract

Cardiovascular and periodontal diseases are common inflammatory conditions in the human population. Tlymphocytesparticipateinthepathogenesis and inflammatory events of atherosclerosis. These immune cells enter theinflamed artery wall and join macrophages via a number of interferon-c-inducible chemokines. Chemokines (IL-8),cytokines, and growth factors also participate in this process. The interaction between interleukin- 8 and its receptor,CXCR2, can also contribute to lesion formation in mice. The main causes of aggressive periodontitis isActinobacillusactinomycetemcomitans. Previous studies have proven that adhesin protein with 24 kDa molecular weight from A.actinomycetemcomitans is a specific adhesin, this adhesin proteins play a role in the adhesion process on host. thiskind adhesion in the epithelial attachment would lead to colonization and invasion ofA.actinomycetemcomitans thatwill stimulate the host immune response. This study aimed to analyze the influence of induction 24 kDaA.actinomycetemcomitans adhesin protein to the titre of IL-8 in heart of Wistar rat with aggressive periodontitis usingElisa method to measure and analyze the titre of IL-8. After analyzed with analysis of variance, showed significantdifferences of IL-8 titre in the control group and the group with the induction byA.actinomycetemcomitans,A.actinomycetemcomitans plus 24 kDa A.actinomycetemcomitans adhesin protein, and only with 24 kDaA.actinomycetemcomitans adhesin protein. It can be concluded that A.actinomycetemcomitans adhesin protein with 24kDa molecular weight has a role in increasing of IL-8 titre in heart wistar rat with aggressive periodontitis.
Ujitoksisitasakut ekstrakbatangpisangambon(Musa paradisiacavar sapientum) terhadap hati mencit (Mus musculus) dengan parameter LD (Acute toxicity test of ambonese banana (Musa paradisiaca var sapientum) stem extract in liver of mice (Mus musculus) with LD 50 parameters 50 Hendrik Setia Budi; Ira Arundina; Retno Indrawati; Leonita Widyana Mahardikasari
Journal of Dentomaxillofacial Science Vol. 13 No. 2 (2014): Formerly Jurnal Dentofasial ISSN 1412-8926
Publisher : DiscoverSys Inc.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15562/jdmfs.v13i2.394

Abstract

The sap of ambonese banana (Musa paradisiaca var sapientum) contain flavonoids, saponins, tannins which have beenwidely used by people in Trunyan Bali as traditional medicine on wound healing, and it has been reported as apotential wound healing after tooth extraction. The aim of this research was to determine the level of safety of usingherbal medicine, ambonese banana stem extract on histopatology liver of mice with LDparameters. This experimentalstudy was performed bythe post test only controlled group design. The sample were 28 mice (Mus musculus) randomlydivided into 4 groups. K group as control group was given aquadest. P1, P2, and P3, as treatment groups were givenambonese banana stem extract with dose 0.42g/20gbw, 2.1g/20gbw, 4.2g/20gbw. The extract was given per-oral withsonde on the first day. On day 3, the mice were terminated, and the livers were microscopically histopathological observed. The observed at 3th50 day, there were no deaths in every groups of mice (K, P1, P2, and P3) on the third day observation. Kruskal Wallis test showed there was not significant difference in histopathological appearance on liverof mice (p=0.771). It wasconcluded that the maximumsafety dose that can still be administered is 0.42g/20gbw followingthe LDparameter. Theacute toxicity test of ambonese banana stem extract did not show necrosis on liver but it showedthe highest simple degeneration than all groups which were given 0.42g/20gbw dose.
againaba Daya Saing Industri Life Sciences di Indonesia Ernawati Pasaribu; Retno Indrawati
Jurnal Aplikasi Statistika & Komputasi Statistik Vol 7 No 2 (2015): Journal of Statistical Aplication and Statistical Computing
Publisher : Pusat Penelitian dan Pengabdian Masyarakat Politeknik Statistika STIS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (814.63 KB) | DOI: 10.34123/jurnalasks.v7i2.22

Abstract

Indonesia is the South East Asia’s largest economy and has a substantial and increasingly inspirational middle class of over 20 million. Indonesia has become an attractive market due to her strongly growing consumer market, especially the middle income segment. The high number of population also indicates the existing potential pool of labour. Life Sciences (LS) industry is widely recognised as the new wave of knowledge-based economy. This study identifies relative position of Indonesia in terms of foreign direct investment (FDI) in LS industry and competitiveness of the LS industry in Indonesia compared with other countries. Based on LS sector, Indonesia has to compete mainly with Portugal, Turkey, Saudi Arabia and Nigeria, while based on LS activities, Argentina and Bulgaria are the main competitors. It also reveals that FDI inflow to LS industry in Indonesia is influenced mainly by inflation and return on investment.
The Effect of Pluchea indica Less Leaves Extract Againts Biofilm of Enterococcus faecalis and Fusobacterium nucleatum In Vitro Agni Febrina Pargaputri; Elly Munadziroh; Retno Indrawati
Denta Journal Kedokteran Gigi Vol 11 No 1 (2017): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Background: Enterococcus faecalis and Fusobacterium nucleatum are the most common bacteria found in infected root canal teeth and most of them often caused failure in endodontic treatments. These bacteria can form biofilm which makes them more resistant against antibacterial agents. Biofilm formation also causes a decrease in antibiotics and antimicrobials sensitivity. Pluchea indica Less leaves is a species of plants that has several chemical properties. It consists of flavonoids and polyphenols which have benefits to inhibit biofilm formation. Because of its benefits, the extract of Pluchea indica Less leaves can be potentially developed as one of sterilization dressing in root canal teeth. Purpose: The aim of this study was to determine biofilm formation inhibition of Pluchea indica Less leaves extract against Enterococcus faecalis and Fusobacterium nucleatum. Materials and Methods: The dilution method was done first to show the Minimum Inhibitory Concentration (MIC) of the extract. The inhibition biolfilm formation was tested using microtitter plate assay by measuring the bacterial biofilm Optical Density (OD) from ELISA reader’s results and using autoagregation assay to show the inhibition of adherance bacteria. The Pluchea indica Less leaves extract concentration used for inhibition of biofilm formation were 100%, 50%, 25%, 12,5%, and 6,25%. Results: The result of biofilm formation inhibition showed that Pluchea indica Less leaves extract were able to inhibit Enterococcus faecalis and Fusobacterium nucleatum’ biofilm formation with strong moderate effect. The autoagregation assay showed a decrease in autoagregation percentation of Enterococcus faecalis and Fusobacterium nucleatum. Conclusions: Pluchea indica Less leaves extract has effect to inhibit biofilm formation of Enterococcus faecalis and Fusobacterium nucleatum.
Best Practice in Early Childhood Development Financial Governance: A Case Study in Indonesia Villages Indrawati, Retno; Komara Ragamustari, Safendrri; Ery Wijaya, Muhammad
Jurnal Pendidikan Usia Dini Vol 15 No 2 (2021): Jurnal Pendidikan Usia Dini Volume 15 Number 2 November 2021
Publisher : Program Studi Magister Pendidikan Anak Usia Dini

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (281.435 KB) | DOI: 10.21009/JPUD.152.07

Abstract

Early childhood development (PAUD) is a fundamental investment that is included in the Sustainable Development Goals (SDGs). Previous research has shown that ECD investment in Indonesia, and its financial governance remains a challenging issue. Through a qualitative approach to a case study method design, this study aims to examine the state of PAUD financial management in Panggungharjo Village, Yogyakarta. This study uses in-depth interviews and desk research as data collection techniques. The instrument was developed from the SABER-ECD World Bank Assessment and ECD Financial Profiling Tools. The results showed that PAUD financial management in established villages had innovative financing, budget management and institutional development resulted in a blueprint for managing PAUD financing in other villages in Indonesia. Important policy objectives in all aspects, including the legal framework, cross-sectoral coordination, program coverage, coverage, equity, data availability, quality standards, and compliance with standards, the vision of financing early childhood development will be more achievable for future research. Keywords: Early Childhood, Financial Governance, Village References: Alatas, H., Brinkman, S., Chang, M. C., Hadiyati, T., Hartono, D., Hasan, A., Hyson, M., Jung, H., Kinnell, A., Pradhan, M., & Roesli, R. (2013). Early childhood education and development services in Indonesia. In Education in Indonesia (pp. 82–108). Institute of Southeast Asian Studies. Ambang.Yudanto. (2019). Analisis Kepemimpinan Transformasional Kepala Desa dalam Pengelolaan Badan Usaha Milik Desa: Studi Kasus Bumdes Panggung Lestari, Bantul [Analysis of Village Head Transformational Leadership in Village Owned Enterprise Management: A Case Study of Bumdes Panggung Lestari, Bantul]. 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