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Characterization of Protease from Bacillus licheniformis F11.1 as a Bio-Detergent Agent Sitti Nur Ilmiah; Nisa Rachmania Mubarik; Budiasih Wahyuntari
Makara Journal of Science Vol 22, No 3 (2018): September
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Proteases are among the most important enzymes in both food and non-food industries taking up almost 60% of the world enzyme market. This enzyme has been used for many industrial processes, especially in the detergent industry. The purpose of this study was to characterize the protease from Bacillus licheniformis F11.1 as a bio-detergent agent. An enzyme assay of protease activity was used to assess and characterize the protease enzyme from B. licheniformis F11.1. It showed that the highest pH protease activity for alkaline protease occurred at pH 8.0 with a value of 35.00 U/mL. Under incubation temperature, the protease had the highest activity at 50 °C with a value of 24.46 U/mL. Protease activity was inhibited by Ca2+, Mn2+, K+, and Na+ ions at concentrations of 5 mM. Protease activity can be enhanced by these ions at concentrations of 2 mM. Protease stability can be measured from half-life. Under an incubation temperature of 50 °C, the half- life of the protease at pH 8, 9, and 10 was 108 min, 114 min, and 98 min, respectively. The assay for enzyme stability with an incubation temperature of 60 °C showed half-lives of 92 minutes, 56 minutes, and 61 minutes for pH 6, 9, and 10, respectively. This enzyme was found to be stable with the addition of detergent compounds such as sodium dodecyl sulfate (SDS), Triton X-100, ethylenediaminetetraacetic acid (EDTA), and hydrogen peroxide; all under low concentrations. Determination of the molecular weight using SDS- PAGE and zymogram found the molecular weight was 32.90-35.16 kDa. These results showed that the alkaline protease from B. licheniformis F11.1 can be used as a bio-detergent because of its tolerance to various detergent compounds.
Karakterisasi α-Amilase dari Aspergillus versicolor 3a1 yang Diproduksi pada Media Limbah Cair Tapioka Aini, Fitratul; Mubarik, Nisa Rachmania; Manaf, Lisdar A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

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Abstract

The aim of this experiment was to characterize A. versicolor 3a1 α-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 α-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of α-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 α-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. α-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 α-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 α-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 α-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 -amylase on cassava waste media. Crude α-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.
Potensi Bakteri Proteolitik Aeromonas caviae NU-4 dan Aeromonas sp. NU-8 sebagai Pengendali Pertumbuhan Microcystis aeruginosa BT-02 pada Ikan Mas (Cyprinus carpio) Mulyeti, Encah Ewi; Rachmania Mubarik, Nisa; Wahjuningrum, Dinamella
0853-8670
Publisher : PBI Yogyakarta

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Abstract

Sianobakteri merupakan kelompok fitoplankton yang umum dijumpai di perairan tawar di seluruh dunia. Sianobakteri menghasilkan toksin mikrosistin yang dihasilkan oleh Microcystis aeruginosa yang menyebabkan kematian ikan. Penelitian ini bertujuan mengetahui potensi bakteri proteolitik yang berasal dari saluran pencernaan ikan nila GIFT dalam menghambat pertumbuhan M. aeruginosa BT-02. Isolat bakteri proteolitik asal saluran pencernaan ikan nila yang menghambat pertumbuhan M. aeruginosa BT-02, yaitu isolat i.e Aeromonas caviae NU-4 and Aeromonas sp. NU-8. Indeks penghambatan bakteri NU-4 (1,71) terhadap M. aeruginosa lebih besar daripada NU-8 (1,34). Mekanisme penghambatan belum diketahui. Aplikasi Microcystis aeruginosa BT-02 pada ikan mas tidak menyebabkan kematian ikan, tetapi menimbulkan beberapa perubahan histopatologi pada hati dan usus ikan mas.Kata kunci: Bakteri proteolitik, Aeromonas sp., Microcystis aeruginosa, ikan mas, tes toksisitas
ISOLASI BAKTERI SELULOLITIK DAN KARAKTERISASI ENZIMNYA Anja Meryandini; Wahyu Widosari; Besty Maranatha; Titi Candra Sunarti; Nisa Rachmania; Hasrul Satria
Makara Journal of Science Vol 13, No 1 (2009): April
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Isolation of Cellulolytic Bacteria and Characterization of the Enzyme. Four of cellulolitic bacteria isolates had been characterized. The determination of cellulase activity was conducted at the highest production time, using crude enzymes with the modification of Miller methods (1959) on pure cellulose substrates such as CMC (Carboxymethyl cellulose), Avicel and Filter paper Whatman No. 1 as well as agriculture waste such as rice straw, corn cob and banana peel. Cellulase from C4-4, C5-1, C5-3 and C11-1 showed optimum activity at pH 5, 70°C, pH 3.5, 90°C, pH 5, 80°C and pH 8, 70°C, respectively. Avicel is a appropriate substrate for C4-4 cellulase whereas CMC for the other three. C11-1 cellulase has the highest cellulase enzyme activity on rice straw substrate whereas C4-4 cellulase on banana peel substrates. C5-1 and C5-3 cellulase have relatively low cellulase activities in degrading substrates of agriculture waste. However, isolates of C5-1 and C5-3 have high cellulase activities on banana peel substrates. Keywords: bacteria, cellulolitic, cellulase, agriculture waste
The Abundance of Nitrogen Fixing, Nitrifying, Denitrifying and Ammonifying Bacteria in the Soil of Tropical Rainforests and Oil Palm Plantations in Jambi Zulfarina Zulfarina; Iman Rusmana; Nisa Rachmania Mubarik; Dwi Andreas Santosa
Makara Journal of Science Vol 21, No 4 (2017): December
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Alterations in the use of land for oil palm plantations can change the domination and activity of soil bacteria. More specifically, alteration in soil microbial communities can directly affect soil ecosystem functioning, particularly with respect to carbon and nitrogen cycles. Nitrogen can be a limiting nutrient, and the availability of nitrogen in the soil environment becomes a major factor in controlling the production of biomass. This research project aimed at studying the abundance of nitrogen-fixing, nitrogen-oxidizing, nitrogen-reducing, and ammonifying bacteria based on their functional genes in the tropical rain forests of Taman Nasional Bukit Duabelas (TNBD) and the oil palm plantations in Sarolangun Jambi. Samples were collected in November 2015. Soil sampling was performed randomly at three points representing each area of ​​the tropical rainforests of TNBD and the seven- to eight-year-old oil palm plantations. Soil samples were collected using a soil sample core from 0–15 cm below the surface with depth strata of 0–5 cm, 5–10 cm, and 10–15 cm. Composite assessment was conducted on samples from each point corresponding to each respective depth strata. Soil samples were stored at -20ºC prior to testing. Microbial abundance was measured using the most probable number (MPN) method. The abundance of microbes that play a role in nitrogen metabolism between strata of 5–10 cm and 10–15 cm does not appear to be different. The highest abundance of microbes in oil palm plantation land in Jambi was found in samples with nitrifying bacteria, later followed by denitrifying, nitrogen-fixing, and ammonifying bacteria. Ultimately, it was found that microbial abundance in oil palm plantations was higher than the corresponding rates in samples from tropical rainforests.
Characterization of Gibberellin Producing Rhizobacteria Isolated from Soil Forest in Banten Hadi Susilo; Nisa Rachmania Mubarik; Triadiati Triadiati
Current Biochemistry Vol. 2 No. 1 (2015)
Publisher : IPB University

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Gibberellin is plant growth regulator that stimulates cell elongation, seed germination, flowering, and fruit ripening. This study was conducted to isolate, identify, and optimize growth media for gibberellins producing- rhizobacteria isolated from rhizosphere soil of “keruing“ (Dipterocarpus sp.) tree in forest research Carita, Pandeglang, Banten. Eight bacterial isolates were obtained and all produced gibberellin. The BC2 isolate produced the highest of gibberellin (0.897 mg mL-1) and then selected for identification based on physiology, molecular character, and effects of growth media with variation of temperature, pH, and light. The result of physiological test indicated that BC2 isolate does not produce indole, positive on urease and oxidative carbohydrate. The phylogenetic analysis showed that BC2 isolate is belonged to Stenotrophomonas maltophilia with 98% similarity level. The optimation of growth media indicated that the growth of BC2 isolate was optimum at 30°C, pH 7, and dark condition.
Detection and Cloning of a Gene Involved in Zwitermicin A Synthesis from Plant Growth Promoting Rhizobacteria of Bacillus sp CR64 Wahyudi, Aris Tri; Astuti, Rika Indri; Mubarik, Nisa Rachmania; Faulina, Sarah Asih
Indonesian Journal of Biotechnology Vol 15, No 1 (2010)
Publisher : Universitas Gadjah Mada

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Abstract

Utilization of soil bacteria as biocontrol agent is becoming popular due to its valuable and effective mechanisms to suppress plant pathogenic microbes. We have previously isolated Bacillus sp, designated as Bacillus sp CR64, which exhibited effective plant growth promoting and antifungal activities. In this study, CR64 was examined in inhibiting the growth of Rhizoctonia solani, the causing agent of root rot disease. Partial sequence analysis of 16S rRNA gene revealed that this isolate similar with Bacillus cereus (94%). Furthermore, a gene designated zmaR was detected by means of specific amplification of DNA fragment approximately 950 bp. This fragment was then cloned onto pCRII-TOPO (3.9 kb) and sequenced using DNA sequencer ABI PRISM 310. Sequence analysis revealed that it had highest homology with the ZmaR protein (89% identity; 90% similarity) of B. thuringiensis serovar kurstaki (AAF82729.2). Alignment analysis with other ZmaR sequences from other antibiotic-producing Bacilli exhibited an almost fully conserved region within ZmaR sequences.Key words : PGPR, Bacillus sp CR64, Zwitermicin A, Cloning, Antifungal.
Isolasi dan Karakterisasi Amilase dari Kapang Alkalotoleran Asal Limbah Cair Tapioka Mubarik, Nisa Rachmania; Damayanti, Evi; Listyowati, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 8, No 1 (2003): February 2003
Publisher : Universitas Atma Jaya Yogyakarta

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A total of 6 fungi isolates of growing at pH 9 and 2 isolates on pH 10 with amylolytic indexes 0f 0.07-1.42 have been isolated from cassava starch liquid waste. Two isolates having the highest amylolytic index were identified as Aspergillus sydowii K10 (1.42) and Aspergillus versicolor L30 (1.4). Both A. sydowii K10 and A. versicolor L30 were described as alcalotolerant for being able to grow with range pH 5-10. The optimal -amylases production of A. sydowii K10 and A. versicolor L30 was obtained after 4 and 3 days of incubation at 300C. The optimum of -amylase activity from A. sydowii K10 was at 400C and 700C, and pH 6; while those from A. versicolor L30 was at 500C and pH 6 respectively. Both A. sydowii K10 and A. versicolor L30 could produce glucoamylase. The optimum of glucoamylase activity from A. sydowii K10 was at 400C and pH 5, while those from A. versicolor L30 was at 500C and pH 5 respectively.
Penapisan dan Identifikasi Bakteri Kitinolitik Penghambat Pertumbuhan Ganoderma boninense in Vitro Risky Hadi Wibowo; Nisa Rachmania Mubarik; Iman Rusmana; Maggy Thenawidjaya
Jurnal Fitopatologi Indonesia Vol. 13 No. 3 (2017)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

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Chitinolytic bacteria have been reported as biocontrol agents and have the ability to produce chitinase enzymes. The objective of the research was to obtain chitinase producing bacteria that had antagonistic activity to Ganoderma boninense, a causal agent of basal stem rot on oil palm. A total of 63 isolates of chitinase producing bacteria were isolated from soil of Bukit Dua Belas National Park and oil palm plantation in Jambi Province; all was screened for their potency in inhibiting G. boninense in vitro. Three isolates designated TB04-05, SW01-11, and SW02-08 were potentially suppressed and inhibited the mycelium growth of G. boninense in vitro. Based on their specific chitinase activity, these three isolates produced the highest level of chitinase enzyme of 6.3072 U mg-1 protein, 6.0385 U mg-1 protein and 6.1279 U mg-1 protein, respectively after 24 hr incubation. Based on 16S RNA identification, strain TB04-05 had similarity with Bacillus cereus, whereas strains SW01 and SW02-08 had similarity with Bacillus thuringiensis.
Pencirian Mananase Streptomyces costaricanus 451-3 Anjani Meryandini; Dwi Ambarawati; Nisa Rachmania
Jurnal Ilmu Pertanian Indonesia Vol. 13 No. 1 (2008): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Major component of hemicelluloses are mannans (softwoods) and xylans (hardwoods). Hemicclluloses arc used by microbes as a carbon sources. Mannanase and xylanasc arc enzyme complex that are able to degrade hemicelluloses. Mannanase activity from Streptomyces costarianus 451-3 was tested in locust bean gum 0.5% and coconut meal 0.5% medium and was detected by dinitrosalysilic acid method. Protein concentration was measured using Bradford method. Mannanase and xylanase activity were also detected using birchwood xylan and oatspelt xylan medium. The optimum temperature and pH of Streptomyces mannanase strain 451-3 was 40 oC and 6,0, respectively. The addition 1mM of Mg2+ and Zn2+ at final concentration increased the mannanase activity for about 30% and 80%, while 1mM Mn2+, Ca2+ and Co2+ decreased its activity for about 67%, 100%, and 60%, respectively. The addition of I mM ethylene diamine tetraacetic acid tend to decreased the enzyme activity to 30%. The medium which contain birchwood xylan dan oatspelt xylan could induce mannanase activity, hut in a lower degree then that of xylanase. Keywords: mananase, streptomyces, xilanasc 
Co-Authors , Triadiati . QATRUNNADA Ahmad Suryadi ANGELIA REZTY FITRIANI SITUMORANG Anggreandari, Rizky Ani Suryani Anja Meryandini Antonius Suwanto dan Meity S. Sinaga . Budi Tjahjono Andi Khaeruni R ARIS TJAHJOLEKSONO Aris Tri Wahyudi Besty Maranatha BRAMANTYO JATI PRASOJO Budiasih Wahyuntari Budiasih Wahyuntari Budiasih Wahyuntari Cahyadi, Alfan Cahyadi, Alfan Dadang Suhendar Delfi Trisnawati DERI YURATMOKO Desniar . Dewi Seswita Zilda Dewi Seswita Zilda Dian Syahfitri Dinamella Wahjuningrum Dini, Isna Rahma Dwi Ambarawati DWI ANDREAS SANTOSA Ekowati Chasanah Encah Ewi Mulyeti Evi Damayanti Evi Damayanti, Evi Fauzy Rachman Fauzy Rachman Ferymon Mahulette Ferymon Mahulette, Ferymon Fitratul Aini Fitratul Aini, Fitratul Galih Cendana Nabilasani Hadi Susilo Hamim Hamim Hana Nurullita Prestisia Hasrul Satria HENDRA PARDEDE Hirmas Fuady Putra HIRMAS FUADY PUTRA, HIRMAS FUADY Idin Abidin Iman Rusmana Ismi Isti'anah Iswati, Ruma It Jamilah Jepri Agung Priyanto Lia Siti Nur'amaliyah Lia Siti Nur'amaliyah LISDAR I. SUDIRMAN Listyowati, Sri lmiah, Sitti Nur Luky Adrianto Maggy Thenawidjaya Maggy Thenawidjaya Suhartono Marini Adani Masrukhin Masrukhin Mutiha Panjaitan Nabilasani, Galih Cendana Nabilasani, Galih Cendana Nurul Hidayati Partomuan Simanjuntak Partomuan Simanjuntak Puspita Lisdiyanti Rika Indri Astuti Risky Hadi Wibowo Risky Hadi wibowo Risky Hadi Wibowo Rizky Anggreandari Ruma Iswati Sarah Asih Faulina Sarah Asih Faulina, Sarah Asih Sipriyadi Sipriyadi Sipriyadi Siswa Setyahadi Siswodarsono, Trismilah Siswodarsono, Trismilah Siti Azzira Rahma Sitti Nur Ilmiah Sonya Tobing Sri Budiarti Poerwanto Sri Listyowati Suhendar, Dadang Suhendar, Dadang SYAMSUL BAHRI SYAMSUL BAHRI TEDJA IMAS Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) TRI ASMIRA DAMAYANTI Tri Handayani Kurniati Trismilah Siswodarsono Wahyu Widosari Widanarni Widanarni Yusro Nuri Fawzya Zulfarina Zulfarina Zulfarina,