Article Per Year (5 Year)
p-Index From 2018 - 2023
2.426
P-Index
This Author published in this journals
All Journal Jurnal Pendidikan dan Pengajaran Journal of Biology Education Biosaintifika: Journal of Biology & Biology Education Mimbar Ilmu Life Science
Ibnul Mubarok
Universitas Negeri Semarang, Semarang, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Education Public Health Social Sciences

Published : 30 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search
Journal : Biosaintifika: Journal of Biology

 1 
ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG -, Jumiyati; Bintari, Siti Harnina; Mubarok, Ibnul
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2265

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.
Biosorption of Heavy Metal Pollution by Enterobacter agglomerans Dewi, Nur Kusuma; Mubarok, Ibnul; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20471

Abstract

Biosorption is a new waste treatment technology that can eliminate toxic heavy metals. Biosorption can be considered as an environmentally friendly alternative technology to treat industrial liquid waste that is economically proper to use. One of them is biosorption that utilizes the microorganisms’ absorption ability, especially bacteria that can absorb heavy metals in waters, such as Enterobacter agglomerans. This research aimed to determine the ability of E. agglomerans in reducing heavy metals pollution in local river. The research employed the measurement of the effect of lead (Pb) to E. agglomerans growth using Optical Density (OD) at wavelength 600 nm. The colony numbers were calculated using a standard curve. While the ability of E. agglomerans to reduce heavy metals concentration in liquid media was measured using AAS with a wavelength of 240 nm. The results showed that lead affected the growth of E. agglomerans. The OD value has a negative relationship with the concentration level of Pb. The ODs were decreased from 2.867 to 1.242, using Pb level from 0 ppm to 20 ppm. Therefore, it proved that E. agglomerans could reduce heavy metals concentration in local river in Central Java Province. This research was the first report on E. agglomerans activity on heavy metal in contaminated water. This result can be used as a reference for industrial sites near the river to treat their wastewater before discharging it to the river body to preserve its water purity.
THE POTENTIAL OF CNIDOSCOLUS CHAYAMANSA ALCHOHOLIC LEAVES EXTRACT AS HYPOLIPIDEMIA AGENT Iswari, Retno Sri; Mubarok, Ibnul; Sasi, Fitri Arum
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (374.617 KB) | DOI: 10.15294/biosaintifika.v12i1.23687

Abstract

Cardiovascular disease caused by hyperlipidemia is the highest case in term of fatality of non-infectious disease in Indonesia. Cnidoscolus chayamansa is well known as a herb that has nutraceutical potential for medicine and likely as a hypolipidemia agent. Therefore, this study aimed to identify the optimal dose of C. chayamansa as a candidate to treat cardiovascular diseases. A total of 30 white rats were acclimatized in the laboratory for 14 days. After that, the rats were grouped into six groups, randomly, which including a healthy control group (K0), a negative control group or hyperlipidemic rats (K-), a positive control group or hyperlipidemia-induced rats that was supplemented with atorvastatin (K+), then a hyperlipidemic rats treated with alcoholic extract of C. chayamansa leaves at doses of 100 mg /kgBB /day for K1, 200 mg/ kgBB/ day for K2 and 400 mg/ KgBB/ day for K3. The results showed that K3 treatment is the most effective and optimum dose for lowering total cholesterol level at 76.81±1.10 mg/dl, triglycerides 72.39±1.66 mg/dl and LDL-C at 21.47±0.58 mg/dl. The aortic histology assessment also showed that the K- group had putative thrombus or plaque in intima, and it was not found in other groups. This research focused on the optimum dose and new usage of C. chayamansa, as an anti-inflammatory in atherosclerosis. By understanding the optimum dose of C. chayamansa, the community can apply and control the herb consumption for their own therapeutic properties. For future application, the herb is potentially developed as anti-atherogenic medicine.
The Potential of Cnidoscolus chayamansa Alchoholic Leaves Extract as Hypolipidemia Agent Iswari, Retno Sri; Mubarok, Ibnul; Sasi, Fitri Arum
Biosaintifika: Journal of Biology & Biology Education Vol 12, No 1 (2020): April 2020
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v12i1.23687

Abstract

Cardiovascular disease caused by hyperlipidemia is the highest case in term of fatality of non-infectious disease in Indonesia. Cnidoscolus chayamansa is well known as a herb that has nutraceutical potential for medicine and likely as a hypolipidemia agent. Therefore, this study aimed to identify the optimal dose of C. chayamansa as a candidate to treat cardiovascular diseases. A total of 30 white rats were acclimatized in the laboratory for 14 days. After that, the rats were grouped into six groups, randomly, which including a healthy control group (K0), a negative control group or hyperlipidemic rats (K-), a positive control group or hyperlipidemia-induced rats that was supplemented with atorvastatin (K+), then a hyperlipidemic rats treated with alcoholic extract of C. chayamansa leaves at doses of 100 mg /kgBB /day for K1, 200 mg/ kgBB/ day for K2 and 400 mg/ KgBB/ day for K3. The results showed that K3 treatment is the most effective and optimum dose for lowering total cholesterol level at 76.81±1.10 mg/dl, triglycerides 72.39±1.66 mg/dl and LDL-C at 21.47±0.58 mg/dl. The aortic histology assessment also showed that the K- group had putative thrombus or plaque in intima, and it was not found in other groups. This research focused on the optimum dose and new usage of C. chayamansa, as an anti-inflammatory in atherosclerosis. By understanding the optimum dose of C. chayamansa, the community can apply and control the herb consumption for their own therapeutic properties. For future application, the herb is potentially developed as anti-atherogenic medicine.
Biosorption of Heavy Metal Pollution by Enterobacter agglomerans Dewi, Nur Kusuma; Mubarok, Ibnul; Yuniastuti, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 2 (2019): August 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i2.20471

Abstract

Biosorption is a new waste treatment technology that can eliminate toxic heavy metals. Biosorption can be considered as an environmentally friendly alternative technology to treat industrial liquid waste that is economically proper to use. One of them is biosorption that utilizes the microorganisms’ absorption ability, especially bacteria that can absorb heavy metals in waters, such as Enterobacter agglomerans. This research aimed to determine the ability of E. agglomerans in reducing heavy metals pollution in local river. The research employed the measurement of the effect of lead (Pb) to E. agglomerans growth using Optical Density (OD) at wavelength 600 nm. The colony numbers were calculated using a standard curve. While the ability of E. agglomerans to reduce heavy metals concentration in liquid media was measured using AAS with a wavelength of 240 nm. The results showed that lead affected the growth of E. agglomerans. The OD value has a negative relationship with the concentration level of Pb. The ODs were decreased from 2.867 to 1.242, using Pb level from 0 ppm to 20 ppm. Therefore, it proved that E. agglomerans could reduce heavy metals concentration in local river in Central Java Province. This research was the first report on E. agglomerans activity on heavy metal in contaminated water. This result can be used as a reference for industrial sites near the river to treat their wastewater before discharging it to the river body to preserve its water purity.
ISOLASI DAN IDENTIFIKASI KHAMIR SECARA MORFOLOGI DI TANAH KEBUN WISATA PENDIDIKAN UNIVERSITAS NEGERI SEMARANG -, Jumiyati; Bintari, Siti Harnina; Mubarok, Ibnul
Biosaintifika: Journal of Biology & Biology Education Vol 4, No 1 (2012): March 2012
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v4i1.2265

Abstract

Tujuan penelitian untuk mengisolasi dan mengidentifikasi khamir secara morfologi di tanah Kebun Wisata Pendidikan Unnes. Penelitian ini merupakan penelitian eksplorasi yang dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Unnes. Populasi penelitian adalah khamir di tanah Kebun Wisata Pendidikan Unnes. Sampel penelitian adalah isolat khamir yang diambil dengan teknik Purposive Sampling yaitu membagi kebun menjadi lima zona dan setiap zona diambil secara acak lima titik pengambilan kemudian dihomogenkan. Sampel dibawa ke Laboratorium untuk dilakukan isolasi, purifikasi dan identifikasi secara morfologi koloni dan sel. Simpulan penelitian ditemukan tujuh isolat khamir dan termasuk ke dalam lima genus yaitu Saccharomyces, Candida, Debaromyces, Brettanomyces dan Saccharomycodes. The purpose of the research was to isolate and to identify morphologically the individually-isolated yeasts from the soil of Biology Educational Garden Semarang State University. The exploration research was conducted in the Microbiology Laboratory Department of Biology, FMIPA Semarang State University. The yeasts were collected using purposive sampling technique in five zones and from each zone five random plots were selected to obtain the samples. Samples were isolated, purified and identified morphologically in terms of the colonies and the cells. Result revealed that seven isolated yeasts from five generas (Saccharomyces, Candida, Debaromyces, Brettanomyces, and Saccharomycodes) had been collected from the soil of Biology Educational Garden Semarang State University.
Co-Authors Afifa Megantara, Afifa Agus Yulianto Agus Yulianto Agus Yulianto Alam, Aulia Nuanza Anhari, Saeful Ari Yuniastuti Bambang Priyono Chulia Mubtadiah, Chulia Dewi Mustikaningtyas DWI SUSILANINGSIH Dyah Rini Indriyanti Eling Purwantoyo Fitri Arum Sasi, Fitri Arum Iwan Junaedi Iwan Junaedi Iwan junaedi Jumiyati - Khairunisa, Nisrina Mahardhika Adhi Pratama, Mahardhika Adhi Martin H. B, F. Putut Mohammad Qois Syafi Mulyati Mulyati Noeryana, Dyah Vita Nuha, Agus Ulin Nur Kusuma Dewi Parmin Parmin Parmin Parmin Parmin Purwaningsih, Sri Dyah Ayu Rahmawati, Ain Amalia Retno Sri Iswari Riska Damayanti, Riska Rizki Oktavia, Rizki Siti Harnina Bintari Sri Sukaesih Sutrisnowati, Intan Indah Sutrisnowati, Intan Indah Syafi, Mohammad Qois