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All Journal Jurnal Hortikultura Jurnal Pendidikan Manajemen Perkantoran (JPManper) These proceedings represent the work of researchers participating in The International Conference on Elementary Education (ICEE) which is being hosted by the Elementary Education Study Programme School of Postgraduate Studies, Universitas Pendidikan
Agus Muharam
Balai Besar Pengkajian dan Pengembangan Teknologi Pertanian Jl. Tentara Pelajar No. 10 Bogor – Jawa Barat
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Teknologi Budidaya Tanaman Tomat Melalui Inverted Gardening dan Conventional Gardening Berbasis Pemanfaatan Bakteri Indigenus Widawati, S; Sudiana, IM; Sukara, E; Muharam, Agus
Jurnal Hortikultura Vol 22, No 3 (2012): September 2012
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Inokulan padat Azzofor-wd3 merupakan campuran 16 isolat bakteri indigenus lahan gambut (Rhizobium,  Azotobacter, Azospirillum, dan bakteri pelarut fosfat) masing-masing empat isolat digunakan sebagai pupuk hayati untuk meningkatkan produksi tomat dalam inverted dan conventional gardening. Penelitian bertujuan mengetahui peran potensial inokulan padat Azzofor-wd3 sebagai plant growth promoter dalam kondisi lingkungan ekstrim, khususnya pada lahan gambut. Penelitian dilaksanakan di Pusat Penelitian Biologi, LIPI,  dari Bulan Januari sampai dengan Desember 2011 Penelitian menggunakan rancangan acak lengkap dengan 28 perlakuan penambahan media pupuk pada media tanam dengan tiga ulangan atau pot. Media dasar ialah gambut (50%) dan tambahan pupuk hayati (50%). Perlakuan tambahan media pupuk mencakup : (1) gambut sebagai kontrol, (2) sekam kotoran ayam, (3) kompos, (4) pasir halus, (5) kapur, (6) Azzofor-wd3, (7) sekam kotoran ayam + pasir halus, (8) sekam kotoran ayam + kapur, (9) sekam kotoran ayam + Azzofor-wd3, (10) kompos + pasir halus, (11) kompos + kapur, (12) kompos + Azzofor-wd3, (13) pasir halus + kapur, (14) pasir halus + Azzofor-wd3, (15) kapur + Azzofor-wd3, (16) sekam kotoran ayam + kompos + pasir halus, (17) sekam kotoran ayam + kompos + kapur, (18) sekam kotoran ayam + kompos + Azzofor-wd3, (19) sekam kotoran ayam + pasir halus + kapur, (20) sekam kotoran ayam + pasir halus + Azzofor-wd3, (21) sekam kotoran ayam + kapur + Azzofor-wd3, (22) kompos + pasir halus + kapur, (23) kompos + kapur + Azzofor-wd3, (24) pasir halus + kapur + Azzofor-wd3, (25) sekam kotoran ayam + kompos + pasir halus, (26) sekam kotoran ayam + kompos + pasir halus + Azzofor-wd3, (27) kompos + pasir halus + kapur + Azzofor-wd3, dan (28) sekam kotoran ayam + kompos + pasir halus + kapur + Azzofor-wd3. Hasil penelitian menunjukkan bahwa produksi tomat tertinggi setelah 3 bulan ialah pada perlakukan media gambut + sekam kotoran ayam + kompos + pasir halus + kapur + Azzofor-wd3 pada inverted dan conventional gardening, masing-masing sebesar 63,9 dan 65,9 g/pot.  Terdapat perbedaan pengaruh perlakukan yang nyata antara inverted dan conventional gardening dalam hal P-tersedia, populasi bakteri, dan aktivitas PME-ase. Namun demikian, tidak ada pengaruh perlakuan yang nyata terhadap produksi tomat antara inverted dan conventional gardening.  Hasil penelitian ini menunjukkan bahwa Azzofor-wd3 merupakan bakteri pendorong pertumbuhan tanaman yang potensial untuk tanaman tomat yang dibudidayakan pada lahan gambut.  Aplikasi jenis bakteri tersebut sangat bermanfaat dalam  pengayaan tanah gambut untuk pembudidayaan tanaman sayuran. ABSTRACT. Widawati, S, Sudiana, IM, Sukara, E, and Muharam, A 2012. The Technology of Tomato Plant Cultivation Through Inverted and Conventional Gardening Based on Utilization of Indigenous Bacteria. Azzofor-wd3 is a solid inoculant consisted of 16 peat indigenous bacteria isolates i.e. Rhizobium, Azotobacter, Azospirillum, and PSB four isolates respectively were used as biofertilizers to stimulate tomato production on inverted and conventional gardening. An experiment was conducted at the Research Center for Biology, Indonesian Institute of Sciences from January until December 2011. The research was aimed to determine the potential role of Azzofor-wd3 solid inoculant as a plant growth promoter in extremely environmental conditions. The treatments of growth media mixture were arranged in a completely randomized design with three replications. The based media was peat for 50% of mixture. The treatments were the addition of biofertilizers with the same volume of the based media (50%). The treatments were (1) peat only as the control, (2) chicken dunk, (3) compost, (4) fine sand, (5) lime, (6) Azzofor-wd3 inoculant, (7) chicken dunk + fine sand, (8) chicken dunk + lime, (9) chicken dunk + Azzofor-wd3, (10) compost + fine sand, (11) compost + lime, (12) compost + Azzofor-wd3, (13) fine sand + lime, (14) fine sand + Azzofor-wd3, (15) lime + Azzofor-wd3, (16)  chicken dunk + compost + fine sand, (17) chicken dunk + compost + lime, (18) chicken dunk + compost + Azzofor-wd3, (19) chicken dunk + fine sand + lime, (20) chicken dunk + fine sand + Azzofor-wd3, (21) chicken dunk + lime + Azzofor-wd3, (22) compost + fine sand + lime, (23) compost + lime + Azzofor-wd3, (24) fine sand + lime + Azzofor-wd3, (25) Chicken dunk + compost + fine sand, (26) chicken dunk + compost + fine sand + Azzofor-wd3, (27) compost + fine sand + lime + Azzofor-wd3, and (28) chicken dunk + compost + fine sand + lime + Azzofor-wd3. The results showed that the highest production of  tomato in inverted gardening was 63.9 g/pot and in conventional gardening was 65.9 g/pot produced by the plants grown on peat + chicken dunk + compost + sand + lime + Azzofor wd3 inoculant, 3 months after planting. There was significant difference of available-P, bacterial population, and PME-ase activity in inverted and conventional gardening before and after fertilization, whereas there was no significant difference of  tomato yield between inverted and convensional gardening. It can be concluded that  Azzofor-wd3 is potential as a plant growth promoting bacteria for tomato plants grown in peat soil. The application of the bacteria is very helpful to enrich peat soil for growing  vegetable crops.
Uji Laboratorium Azospirillum sp. yang Diisolasi dari Beberapa Ekosistem Widawati, S; Muharam, Agus
Jurnal Hortikultura Vol 22, No 3 (2012): September 2012
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Beberapa mikrob yang bersifat nonpatogenik dan nonsimbiotik yang efektif menambat nitrogen dari udara serta mampu melarutkan P terikat pada Ca, Al, dan Fe dalam tanah, dapat hidup dalam berbagai ekosistem di alam. Sebagian bakteri tersebut dapat diisolasi dari daerah perakaran tanaman hortikultura. Penelitian bertujuan mengetahui peran Azospirillum sp. yang potensial sebagai pendorong pertumbuhan tanaman pada ekosistem pantai dan kondisi lingkungan yang ekstrim. Pengujian terhadap isolat bakteri yang dikumpulkan dari berbagai kondisi ekosistem dilaksanakan di Laboratorium Ekofisiologi, Pusat Penelitian Biologi, LIPI, Bogor dari Bulan Januari sampai dengan Desember 2011. Sebanyak 34 isolat Azospirillum sp. diuji dengan berbagai metode, yaitu (1) uji kualitatif kemampuan isolat Azospirillum sp.  dalam menambat (fiksasi) nitrogen dan kemampuan hidup pada media Okon padat yang mengandung NaCl, (2) uji kualitatif kemampuan isolat Azospirillum sp. dalam melarutkan P terikat pada  Ca3(PO4)2 dalam media  Pikovskaya padat dan indeks efisiensi pelarutan fosfat,  (3) uji kualitatif kemampuan isolat Azospirillum sp. dalam melarutkan P terikat pada media  Pikovskaya cair  dan aktivitas enzim PME-ase asam dan basa, serta kondisi pH selama inkubasi 7 hari pada kultur murni (pH asal= 7), dan (4) analisis kemampuan Azospirillum sp. dalam memproduksi indole acetic acid (IAA).  Hasil penelitian menunjukkan bahwa: (1) semua isolat bakteri yang diuji mampu menambat nitrogen dalam media Okon padat,  (2) isolat B2, B4, B6, B12,  B14, PS2, dan FR13 mampu melarutkan P dari Ca3(PO4)2 dalam medium Pikovskaya padat dengan masing-masing indeks efisiensi pelarutan sebesar  120, 160, 140, 100, 110, 120, dan 100,  (3) isolat B1, B2, B3, B4, B6, B14, B17, PS1, PS2, PS3, FR1, FR5, FR7, FR8, FR10, FR12, dan FR13 mampu tumbuh dalam medium Okon dengan kandungan NaCl sebesar 0, 2, 4, atau 6%, (4)  konsentrasi tertinggi P terlarut dihasilkan oleh isolat B4 (5,80 mg/l), B6 (5,84 mg/l), dan PS2 (5,45 mg/l) dengan PME-ase sebesar 0,58 u m/l, 0,58 u m/l, 0,57 u m/l (asam), 0,52 mg/l, 0,50 mg/l, 0,48 mg/l (basa), dan dengan  pH : 4,20, 4,30, dan 4,22,  dan (5) isolat B4 dan B6 yang diisolasi dari pertanaman padi di pantai Rambut Siwi, Bali, mampu memproduksi IAA tertinggi, yaitu masing-masing sebesar 0,6749 dan 0,4694 ppm pada hari pertama setelah perlakuan. Berdasarkan hasil penelitian ini terbukti bahwa isolat Azospirillum sp. berpotensi sebagai plant growth promoter  untuk ekosistem di daerah pesisir atau pantai. Bakteri tersebut sangat penting untuk pengkayaan nutrisi pada lahan di daerah dataran rendah atau pantai dalam rangka pengembangan tanaman termasuk komoditas hortikultura.ABSTRACT. Widawati, S and Muharam, A 2012. The Laboratory  Test of  Azospirillum sp. Isolated  from Several  Ecosystems. Microbes that are nonpathogenic  and nonsymbiotic bacteria which are effectively fixed up nitrogen from air, and are able to dissolve phosphated bounded on Ca, Al, and Fe in soil, are able to growth in different ecosystems in nature. Some of the bacterial species can be isolated from rizosphere of horticultural crops. The research was aimed to determine the potential role of Azospirillum sp.  as a plant growth promoter in coastal ecosystem and extremely environmental conditions. The laboratory test of Azospirillum sp. isolated from several ecosystems was carried out in the Ecophysiology Laboratory, Research Center for Biology, Indonesian Institute of Sciences, Bogor from January until December 2011. Thirty-four isolates of Azospirillum sp. (B1 to B17;PS1 to PS3; FR1 to FR 14) were investigated with some methods i.e. (1) the qualitative test of the capability of Azospirillum sp. to fix up nitrogen in solid Okon medium containing NaCl, (2) the qualitative test of the capability of Azospirillum sp. in dissolving bounded P in solid Pikovskaya medium and phosphate dissolution efficiency index,  (3) the qualitative test of the capability of Azospirillum sp. in dissolving bounded P in liquid Pikovskaya medium and the activity of acid and base PME-ase, and pH condition after 7 days incubation in pure media, and (4) analysis of the capability of Azospirillum sp. in producing indole acetic acid (IAA).  The results pointed out that : (1) all tested isolates of Azospirillum sp. were  capable to fix up nitrogen in solid Okon medium, (2) isolates of B2, B4, B6, B12,  B14, PS2, and FR13 were capable to solubilize P on Ca3(PO4)2 in solid Pikovskaya medium with its efficiency of  120, 160, 140, 100, 110, 120, and 100, respectively, (3) isolates of B1, B2, B3, B4, B6, B14, B17, PS1, PS2, PS3, FR1, FR5, FR7, FR8, FR10, FR12, and FR13 were able to grow in Okon medium with 0, 2, 4, or 6% of NaCl doses, (4) the highest concentrations of solubilized P was resulted by isolates B4 (5.80 mg/l), B6 (5.84 mg/l), and PS2 (5.45 mg/l) with PME-ase i.e. 0.58 u m/l, 0.58 u m/l, 0.57 u m/l (acid), 0.52 mg/l, 0.50 mg/l, 0.48 mg/l (base), and with pH : 4.20, 4.30, and 4.22, and (5) isolates of B4 and B6 isolated from rice field at Rambut Siwi beach, Bali, were capable to produce highest IAA hormone i.e. 0.6749 and 0.4694 ppm respectively  on the first day after the treatment. Based on the result of this experiment it can be concluded that Azospirillum sp. is a potential plant growth promoting Rhizobacteria for coastal ecosystem. The bacterial species is very important to enrich coastal areas for crop cultivation, including horticulture.
Kompatibilitas Bacillus subtilis, Pseudomonas fluorescens, dan Trichoderma harzianum untuk Mengendalikan Ralstonia solanacearum pada Tanaman Kentang Marwoto, Budi; -, Hanudin; -, Hersanti; Muharam, Agus
Jurnal Hortikultura Vol 22, No 2 (2012): Juni 2012
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Dalam usaha budidaya kentang dijumpai berbagai kendala yang menekan produktivitas tanaman.  Salah satu kendala yang paling penting yaitu patogen tular-tanah yang disebabkan oleh bakteri Ralstonia solanacearum E. F. Smith. Hasil pengamatan di lapangan menunjukkan bahwa patogen ini dapat menimbulkan kehilangan hasil tanaman kentang  40–100%. Salah satu alternatif yang paling prospektif dalam mengendalikan R. solanacearum ialah dengan mengaplikasikan mikrob antagonis yang diisolasi dari alam. Bacillus subtilis, Pseudomonas fluorescens, dan Trichoderma harzianum merupakan mikrob antagonis yang mampu mengendalikan patogen yang terbawa tanah sampai 70% dan mampu meningkatkan produksi tanaman sampai 40%. Penelitian ini bertujuan mendapatkan informasi kompatibilitas mikrob antagonis dan dapat mengendalikan R. solanacearum pada tanaman kentang.  Penelitian dilaksanakan di Laboratorium Fitopatologi Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran, Rumah Kaca Balai Penelitian Tanaman Sayuran, dan Laboratorium Bakteriologi Balai Penelitian Tanaman Hias,  pada bulan Maret sampai dengan Desember  2009. Dari hasil penelitian ini diperoleh dua nomor isolat bakteri yaitu P. fluorescens yang diisolasi dari rizosfer krisan, Segunung (Pf2), B. subtilis berasal dari IPB (Bs 12), dan satu isolat T. harzianum berasal dari Universitas Gadjah Mada (Th 1) kompatibel pada media yang banyak mengandung protein (King’s B), tetapi tidak kompatibel pada media yang banyak mengandung karbohidrat (potato dextrose agar). Indikatornya ialah indeks kompatibilitas ≤1. Aplikasi B. subtilis secara tunggal merupakan perlakuan yang paling efektif mengendalikan R. solanacearum pada kentang. Hal tersebut ditunjukkan oleh persentase penekanan perlakuan tersebut paling besar (35,27%) bila dibandingkan dengan perlakuan lainnya, kecuali perlakuan gabungan antara Bs 12, Pf 2, dan Th 1 yang sama-sama menunjukkan persentase penekanan sebesar 35,27%.ABSTRACT. Hanudin, Marwoto, B, Hersanti, and Muharam, A 2012. Compatibilities of Bacillus subtilis, Pseudomonas fluorescens, and Trichoderma harzianum to Control Ralstonia solanacearum on Potato.   Among the vegetable crops cultivated by farmers, potato is the most important one.  In cultivating the crop, farmers has faced many problems, the most important one is wilt disease caused by Ralstonia solanacearum E. F. Smith.   Based on the available data known that the disease reduces 40–100% of the total production.  One of the alternative methods control which was more environmentally friendly applied by the use of antagonistic microbes isolated from the soil.  Bacillus subtilis, P. fluorescens, and T. harzianum were abundance in the soil and easily be isolated from rhizosfer.  The bacteria are reported to be effective to control soilborn pathogens in the field.  Based on the preliminary research proven that the bacteria could reduce 70% of the total soilborn pathogens in the soil and increase crop production up to 40%.  The objective of  this study was to determine information of compatibilities between antagonistic microbe and bacterial wilt which had control on potato. The research  were conducted in Laboratory and  Greenhouse of the Padjadjaran University, Indonesian Vegetable Research Institute, and Indonesian Ornamental Crops Research Institute, on March to December 2009. The results showed that  isolates of P. fluorescens (Pf2), B. subtilis (Bs12),  and T. harzianum (Th1) were compatible on medium which containing protein (King’s B), but were not copmatible on medium containing carbohydrate (PDA). The indicator is compatibility index ≤1. Single application of B. subtilis  was  effective to  control  R. solanacearum on potato, suppression wilt biggest (35,27%), if compared with other treatments except combination between Bs 12, Pf 2, and Th 1 treatments was 35,27 % too. 
Pengaruh Kompos yang Diperkaya Bakteri Penambat Nitrogen dan Pelarut Fosfat terhadap Pertumbuhan Tanaman Kapri dan Aktivitas Enzim Fosfatase dalam Tanah Widawati, S; Suliasih, Suliasih; Muharam, Agus
Jurnal Hortikultura Vol 20, No 3 (2010): September 2010
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Penggunaan pupuk organik dalam budidaya sayuran memiliki beberapa keuntungan, terutama untukmempertahankan kondisi tanah dan menekan penggunaan pupuk anorganik. Penelitian dilaksanakan di DesaCidawu (1.250 m dpl.), Cibodas, Kabupaten Cianjur, Provinsi Jawa Barat, mulai bulan Januari sampai Desember2007. Sampel tanah dikoleksi dari daerah berbeda di Pontianak, Kalimantan Barat. Penelitian bertujuan mengetahuipengaruh kompos yang diperkaya dengan bakteri pelarut fosfat (phosphate solubilizing bacteria=PSB) terhadappertumbuhan tanaman kapri dan aktivitas enzim fosfatase di dalam tanah. Penelitian menggunakan rancangan acakkelompok dengan lima perlakuan dan tiga ulangan. Varietas kapri yang digunakan ialah varietas lokal. Perlakuanterdiri atas (A) tanpa pupuk, (B) pupuk anorganik (TSP+KCl+Urea), (C) kotoran ayam + sekam, (D) kompos, dan(E) kompos plus, yaitu kompos yang diberi campuran bakteri, yaitu bakteri penambat nitrogen simbiotik, bakteripenambat nitrogen nonsimbiotik, dan bakteri pelarut fosfat. Hasil penelitian menunjukkan bahwa campuran bakteriyang diisolasi dari tanah gambut di Kalimantan Barat yang terkandung dalam kompos plus dapat beradaptasi denganbaik pada lahan di lokasi penelitian. Populasi total SNFB, NSNFB, dan PSB meningkat masing-masing menjadi 9,15x 108, 9,34 x 108, dan 9,35 x 108 sel/g tanah. Keberadaan campuran bakteri tersebut mampu meningkatkan aktivitasenzim fosfatase asam dan basa di dalam tanah. Peningkatan tertinggi aktivitas enzim fosfomonoesterase asam danbasa dicapai pada perlakuan kompos plus. Perlakuan tersebut juga meningkatkan berat buah kapri dibandingkandengan kontrol (75,32 %), perlakuan pupuk kimia (45,48%), kotoran ayam + sekam ( 31,19 %), dan kompos (15,60%). Pemanfaatan campuran bakteri pelarut fosfat tersebut dalam kompos diharapkan dapat digunakan secara meluasdalam pembudidayaan kapri dalam sistem organic farming (OF), sehingga berperan dalam peningkatan produksi dansekaligus menekan penggunaan pupuk anorganik.ABSTRACT. Widawati, S., Suliasih, and A. Muharam. 2010. The Effect of Compost Enriched with SymbioticNitrogen Fixing and Phosphate Solubilizing Bacteria on the Growth of Peas and the Activity of PhosphataseEnzymes in the Soil. The use of organic materials on cultivation of vegetable crops has some advantages, especiallyfor maintaining suitable soil conditions and decreasing the utilization of inorganic fertilizers. The research wascarried out at Cidawu Village, Cibodas (1,250 m asl.), Cianjur, West Java, from January to December 2007. Soilsamples were collected from some different areas in Pontianak, West Kalimantan. The research was aimed todetermine the effect of compost enriched with mixed phosphate solubilizing bacteria (PSB) on the growth of peasand on the activity of phosphatase enzymes in the soil. A randomized block design with five treatments and threereplications was used in the experiment. A local variety of peas was utilized in the experiment. The treatments werethe addition of fertilizers consisted of (A) without any fertilizer, (B) with inorganic fertilizers i.e. TSP+KCl+Urea,(C) with chicken dung + rice husk, (D) with compost, and (E) with compost plus (compost enriched with symbioticnitrogen fixing bacteria/SNFB, nonsymbiotic nitrogen fixing bacteria/NSNFB, and PSB). The results showed thatthe mixed bacteria isolated from peat soil in West Kalimantan in the compost plus (treatment E) properly adaptedin soil conditions at the site of the experiment. The total populations of SNFB, NSNFB, and PSB were increased upto 9.15 x 108, 9.34 x 108, and 9.35 x 108 cell/g soil, respectively. The occurrence of the mixed bacteria increased theactivity of acid and alkaline phosphatases in the soil. The highest activities of acid and alkaline phosphomonoesteraseenzymes in the soil achieved by the treatment of compost plus. The treatment increased the fresh weight of peascompared to control (75.32 %), to the chemical fertilizers (45.48%), to chicken dung+rice husk ( 31.19 %), and tocompost (15.60 %). The widely application of the mixed PSB in compost is hopefully established in cultivation ofpeas in the organic farming (OF) system, then it will confidently support on the increase of peas production, and thedecrease of inorganic fertilizers as well.
Aplikasi Pupuk Organik dan Bakteri Pelarut Fosfat untuk Meningkatkan Pertumbuhan Tanaman Tomat dan Aktivitas Mikroba Tanah Suliasih, Suliasih; Widawati, S; Muharam, Agus
Jurnal Hortikultura Vol 20, No 3 (2010): September 2010
Publisher : Indonesian Center for Horticultural Research and Development

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ABSTRAK. Pemanfaatan bahan organik untuk budidaya tomat dewasa ini difokuskan untuk menekan penggunaanbahan kimia yang berlebihan, sehingga kerusakan lingkungan dapat diminimalkan. Percobaan dilaksanakan di DesaCidawu, Cibodas, Cianjur (1.250 m dpl.), sejak Januari sampai Desember 2007. Pupuk organik yang digunakanyaitu kompos dan kotoran ayam + sekam, sedangkan pupuk hayati yaitu bakteri pelarut fosfat (phosphatesolubilizing bacteria = PSB). Tujuan percobaan adalah mendapatkan inokulan yang efektif untuk meningkatkanpertumbuhan dan hasil tomat, serta menstimulasi aktivitas mikroba dan enzim fosfatase di dalam tanah. Percobaanmenggunakan rancangan acak kelompok dengan lima perlakuan, yaitu tanpa pemupukan maupun inokulan (K),pemberian inokulan PSB (P1), pemberian kompos (P2), pemberian kotoran ayam+sekam (P3), dan pemberianpupuk kimia NPK (P4), masing-masing dengan tiga kali ulangan. Varietas tomat yang digunakan adalah Gondol.Hasil percobaan menunjukkan bahwa pemberian pupuk organik atau inokulan PSB dapat meningkatkan pertumbuhantanaman tomat dan hasil buahnya, serta populasi PSB dan aktivitas enzim fosfatase di dalam tanah. Pemberianinokulan PSB menghasilkan peubah tinggi tanaman tomat tertinggi (108,3 cm) dibandingkan dengan kontrol (72,3cm), dan meningkatkan hasil buah sebanyak 88,2% dibandingkan dengan kontrol, dan juga lebih tinggi daripadapemberian pupuk kimia NPK. Peningkatan tertinggi populasi PSB dan aktivitas enzim fosfatase asam serta basadalam tanah setelah panen tomat juga diperoleh dari pemberian inokulan PSB dibandingkan dengan perlakuanlain. Implementasi inokulan PSB dan pupuk organik secara meluas dalam pembudidayaan tomat diharapkandapat mendorong peningkatan produksi, produktivitas, dan mutu buah tomat untuk memenuhi kebutuhan pasar.ABSTRACT. Suliasih, S. Widawati, and A. Muharam. 2010. The Application of Organic Fertilizers andPhosphate Solubilizing Bacteria to Increase the Growth of Tomato and Soil Microbial Activities. The use oforganic matters on tomato cultivation is focused to decrease the utilization of chemical substances for minimizingenvironmental degradation. An experiment was conducted at Cidawu Village, Cibodas, Cianjur (1,250 m asl.) todetermine the suitable and effective inoculant to increase the growth of tomato plants, and also to stimulate soil microbialactivities. The tomato variety used was Gondol. The organic fertilizers were compost, chicken dung plus rice husk,and phosphate solubilizing bacteria (PSB), as a biofertilizer. A randomized block design with three replications wasutilized in this experiment. The treatments consisted of without organic fertilizers or PSB (control) (K), inoculationof PSB (P1), application of compost (P2), (4) application of chicken dung plus rice husk (P3), and application of thechemical fertilizer (NPK) (P4). The results showed that the application of organic fertilizers and PSB increased thegrowth of tomato plants and also PSB population, acid, and alkaline phosphatase activities in the soil after harvesting.The inoculation of PSB resulted in heighest plant height (108.3 cm) compared to control (72.3 cm) and also thehighest tomato yield compared to the control treatment (88.2%), and even it was higher than the yield caused by theapplication of the chemical fertilizer. The highest increase of PSB population and the activities of acid and alkalinephosphatase enzymes in soil after harvesting was also caused by the application of the PSB inoculant compared to theother treatments. The applications of organic fertilizers and PSB for wide scale cultivation of tomato expectantly playan important role for increasing production, productivity, and quality of tomato to fulfill market demand for the product.
Pengaruh Pemupukan dan Tumpangsari antara Tomat dan Kubis terhadap Populasi Bemisia tabaci dan Insiden Penyakit Virus Kuning pada Tanaman Tomat Setiawati, Wiwin; Gunaeni, Neni; Subhan, -; Muharam, Agus
Jurnal Hortikultura Vol 21, No 2 (2011): JUNI 2011
Publisher : Indonesian Center for Horticultural Research and Development

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Pola tanam sayuran secara tumpang sari telah dimanfaatkan secara meluas di sentra-sentra produksi  sayuran di Indonesia. Penelitian ini bertujuan mengetahui pengaruh pemupukan dan tumpangsari antara tomat dan kubis terhadap populasi Bemisia tabaci dan serangan penyakit virus kuning yang disebabkan oleh virus gemini pada tanaman tomat. Penelitian dilaksanakan di Kebun Percobaan Balai Penelitian Tanaman Sayuran, Lembang (1.250 dpl.) dari bulan Juni sampai dengan Oktober 2008. Rancangan yang digunakan ialah acak kelompok pola faktorial dengan empat ulangan. Dua faktor perlakuan yang diuji, yaitu (1) dosis pupuk (N 180 kg/ha + P2O5 150 kg/ha + K2O 100 kg/ha,  N 168 kg/ha + P2O5 146,5 kg/ha + K2O  145 kg /ha, serta  N 210 kg/ha + P2O5 183,125 kg/ha + K2O 181,25 kg/ha) dan (2) cara tanam (monokultur tomat dan tumpangsari tomat dengan kubis). Hasil penelitian menunjukkan bahwa penggunaan dosis pupuk yang tinggi dan tanaman tomat yang ditanam secara monokultur dapat meningkatkan populasi kutukebul dan serangan penyakit virus kuning dibandingkan dengan dosis pupuk yang lebih rendah. Penggunaan dosis pupuk yang tinggi tidak berpengaruh nyata terhadap peningkatan produksi tomat. Penggunaan dosis pupuk N 168 kg/ha + P2O5  146,5 kg/ha + K2O 145 kg/ha dan tumpangsari tomat dengan kubis dapat direkomendasikan sebagai komponen teknologi PHT untuk pengelolaan hama B. tabaci dan penyakit virus kuning pada tanaman tomat.The intercropping planting technique is widely implemented in vegetable production centers in Indonesia. The research on the application of different doses of fertilizers (N, P, and K) and the planting technique of tomato and cabbage  on B. tabaci and the yellow disease caused by gemini virus was carried out at the Indonesian  Vegetables Research Institute from June to October 2008. The objective was to determine the effect of different doses of fertilizers (N, P, and K) and tomato-cabbage intercropping on the population densities of B. tabaci and incidence of gemini virus on tomato.  A factorial randomized block design with two factors and four replication was used in the experiment. Two treatments factor were tested i.e.  (1) different doses of fertilizers (N 180 kg/ha + P2O5 150 kg/ha + K2O 100 kg/ha,  N 168 kg/ha + P2O5 146,5 kg/ha +  K2O 145 kg/ha, and  N 210 kg /ha +  P2O5 183,125 kg/ha + K2O 181,25 kg/ha), and (2) planting techniques (monoculture and tomato-cabbage intercropping). The result indicated that heigher doses of fertilizers resulted in higher population of whitefly per leaf and yellow virus symptoms on tomato compared to lower doses. Higher amounts of fertilizers did not significantly affect tomato yield. It is suggested that the dose of  N 168 kg/ha + P2O5 146,5 kg/ha + K2O 145 kg/ha, and the tomato-cabbage intercropping technique can be incorporated into the IPM program, especially for the management of  whitefly and gemini virus on tomato.
Insiden Penyakit Virus Tular Umbi pada Tigabelas Varietas Bawang Merah Asal Jawa Barat dan Jawa Tengah Gunaeni, Neni; Wulandari, Astri W; Duriat, Ati Srie; Muharam, Agus
Jurnal Hortikultura Vol 21, No 2 (2011): JUNI 2011
Publisher : Indonesian Center for Horticultural Research and Development

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Penyakit virus tular umbi merupakan salah satu kendala dalam meningkatkan produksi bawang merah. Hal ini disebabkan oleh virus yang infeksinya bersifat sistemik. Apabila partikel virus berada dalam jaringan benih umbi, maka akan sulit untuk dikendalikan dan dapat membawa masalah baru pada pertanaman berikutnya. Penelitian bertujuan mengetahui insiden penyakit virus tular umbi pada 13 varietas bawang merah yang berasal dari Jawa Barat dan Jawa Tengah. Penelitian dilaksanakan di Rancaekek (elevasi 650 m dpl.) dan di Laboratorium Balai Penelitian Tanaman Sayuran Lembang (elevasi 1.250 m dpl.), sejak bulan Agustus sampai November 2004. Perlakuan terdiri atas 13 varietas bawang merah, yaitu: Lodra, Sumenep, Batu, Merah Maja, Merah Cigugur, Ciniru, Bima, Bima Curut, Bima Timor, Bima Arjuna, Kuning Tablet, Kuning Gombong, dan Philipina. Rancangan yang digunakan ialah acak kelompok dengan tiga ulangan. Hasil penelitian menunjukkan bahwa (1) insiden penyakit virus tular umbi pada masing-masing varietas bawang merah asal Jawa Barat dan Jawa Tengah berturut-turut yaitu varietas Lodra 84,67%, Sumenep 82,56-100%, Batu 39,86-78,67%, Merah Maja 95,25%, Merah Cigugur 100%, Ciniru 66,27%, Bima Curut 78,57%, Bima 100%, Bima Timor 57,98%, Bima Arjuna 47,96%, Kuning Tablet 57,48%, Kuning Gombong 97,92%, dan Philipina 97,92 %, (2) gejala infeksi virus pada daun umumnya berupa  klorosis, mosaik bergaris kuning vertikal terputus-putus, garis-garis hijau vertikal, dan ukuran daun menjadi kecil, (3) gejala-gejala tersebut bereaksi positif dengan OYDV(onion yellow dwarf virus) dan SYSV (shallot yellow stripe virus) berdasarkan uji DAS-ELISA (double antibody sandwich-enzyme linked immunosorbent assay). Informasi mengenai insiden virus tular umbi pada bawang merah ini sangat penting dalam rangka mengembangkan metode perbenihan bawang merah bebas virus. Virus disease is one of major problems in increasing shallots production, because its infection has a systemic character. If it is already in shallots bulb tissues, the virus is difficult to be controlled and will cause new problems to the next planting. The experiment was aimed to determine incidence of bulb-borne virus diseases on  thirteen varieties of shallots (Allium cepa var. ascalonicum) originated from West  and Central Java. The experiment was carried out at Indonesian Vegetable Research Institute Lembang (1,250 m asl.) and Rancaekek (650 m asl.), from  August to November 2004. The shallot varieties tested were Lodra, Sumenep, Batu, Merah Maja, Merah Cigugur, Ciniru, Bima, Bima Curut, Bima Timor, Bima Arjuna, Kuning Tablet, Kuning Gombong, and Philipina. A randomized complete block design with three replications were used in this experiment. The results of the experiment showed that  (1) incidence of virus diseases in shallots bulb on variety Lodra was 84.67%, Sumenep 82.56-100%, Batu 39.86-78.67%, Merah Maja 95.25%, Merah Cigugur 100%, Ciniru 66.27%, Bima Curut 78.57%, Bima 100%, Bima Timor 57.98%, Bima Arjuna 47.96%, Kuning Tablet 57.48%, Kuning Gombong 97.92%,  and Philipina 97.92 %, (2) the virus symptoms exhibited on infected shallots were  yellow stripe mosaic, chlorosis,  green stripe leaf,  and leaves became small, and (3) the symptoms were associated with OYDV (onion yellow dwarf virus) and SYSV (shallots yellow stripe virus) base on DAS-ELISA (double antibody sandwich-enzyme linked immunosorbent assay). Information on the incidence of viral diseases on shallots bulb is very important to develop the production technology of virus-free shallots bulb.
Sifat Inovasi dan Aplikasi Teknologi Pengelolaan Terpadu Kebun Jeruk Sehat dalam Pengembangan Agribisnis Jeruk di Kabupaten Sambas, Kalimantan Barat Kahmir, Hilmi Ridwan; ruswandi, Agus; Winarno, -; Muharam, Agus; Hardiyanto, -
Jurnal Hortikultura Vol 18, No 4 (2008): Desember 2008
Publisher : Indonesian Center for Horticultural Research and Development

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Untuk meningkatkan produksi dan mutu buah jeruk di Indonesia, Pusat Penelitian dan Pengembangan Hortikultura telah melaksanakan program penelitian dan pengkajian penerapan pengelolaan terpadu kebun jeruk sehat (PTKJS) di beberapa provinsi sentra produksi jeruk. Pengelolaan terpadu kebun jeruk sehat meliputi (a) penggunaan bibit jeruk berlabel bebas penyakit, (b) pengendalian OPT terutama vektor penyakit CVPD, (c) sanitasi kebun yang baik, (d) pemeliharaan tanaman secara optimal, dan (e) konsolidasi pengelolaan kebun. Tujuan penelitian adalah mengetahui sifat inovasi teknologi PTKJS yang berpengaruh terhadap adopsi inovasi oleh petani. Penelitian dilaksanakan di Kabupaten Sambas, Kalimantan Barat, dari bulan April sampai Desember 2006, menggunakan metode survei. Hasil penelitian menunjukkan bahwa tidak semua inovasi teknologi PTKJS diadopsi oleh petani jeruk di Kabupaten Sambas. Inovasi teknologi yang tidak diadopsi oleh petani memiliki sifat inovasi yang berkategori nilai rendah. Inovasi teknologi tersebut adalah penggunaan perangkap kuning, penyiraman tanah dengan insektisida, penggunaan sex feromon, pemberongsongan, penyulaman dengan bibit berlabel, pemangkasan arsitektur, penyiraman tanaman dan pemanenan secara benar, serta konsolidasi pengelolaan kebun. Inovasi teknologi PTKJS yang sangat cepat diadopsi oleh petani adalah penyaputan batang menggunakan bubur (belerang) Kalifornia. Faktor nonteknis yang mempengaruhi adopsi teknologi PTKJS adalah kurang dukungan benih bermutu dari instansi berwenang, kurang dukungan penyediaan input produksi, khususnya belerang, rendahnya harga jual, dan kurangnya modal finansial petani.ABSTRACT. Ridwan, H.K., A. Ruswandi, Winarno, A. Muharam, and Hardiyanto. 2008. I���v�����Innovation Characteristics and Technologies Application of Integrated Crop Management for Healthy Citrus Orchid (ICMHCO) on the Development of Citrus Agribusiness in Sambas District, West Kalimantan. To increase production and quality of citrus in Indonesia, the Indonesian Center for Horticulture Research and Development has conducted research and assessment program of ICMHCO in several provinces. Integrated Crop Management for Healthy Citrus Orchid consists of (a) labelled and free diseases planting materials use, (b) pests and diseases control especially for the CVPD vector, (c) good field sanitation, (d) optimum cultural practices, and (e) field management consolidation. The objectives of this research was to evaluate characteristics of ICMHCO innovatory technologies that affect the adoption of the innovations by the farmers. The research was conducted in Sambas District, West Kalimantan, from April to December 2006, using survey method. The results showed that only a part of the innovatory technologies of ICMHCO were adopted by the citrus farmers in Sambas District. The non-adopted technologies generally have low value of technology characteristics. The technologies were labelled free diseases planting materials, yellow trap application, drenching with insecticide solutions, sex pheromon application, pruning, fruit wraping, irrigation and good harvesting practices. The innovatory technologies promptly adopted by the citrus farmers was California (sulphur) paste application. Non-technical factors that affect the adoption of the innovatory technologies of ICMHCO were less quality seed support institutionally, less support of production input from the proper institution especially for sulphur, low selling price and less financial capital of the farmers.
Proteksi Silang untuk Pengendalian Virus Mosaik Mentimun pada Krisan Rahardjo, Indiarto Budi; Muharam, Agus; Sulyo, Yoyo
Jurnal Hortikultura Vol 15, No 2 (2005): Juni 2005
Publisher : Indonesian Center for Horticultural Research and Development

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Salah satu virus yang menyerang tanaman krisan adalah CMV. Alternatif pengendalian CMV pada krisan adalah menggunakan vaksin CARNA 5. Tujuan penelitian ini adalah mengetahui teknik aplikasi vaksin yang paling efektif dalam memproteksi CMV pada krisan. Penelitian dilaksanakan di Laboratorium Virologi Balai Penelitian Tanaman Hias, Segunung dari bulan Januari sampai Desember 2001. Percobaan menggunakan rancangan acak kelompok tiga ulangan dengan delapan perlakuan, yaitu (1) penyambungan, (2) inokulasi mekanis vaksin CARNA 5 dengan konsentrasi 5 μg/ml, (3) inokulasi mekanis vaksin CARNA 5 dengan konsentrasi 15 μg/ml, (4) inokulasi mekanis vaksin CARNA 5 dengan konsentrasi 20 μg/ml, (5) inokulasi mekanis vaksin CARNA 5 dengan konsentrasi 25 μg/ml, (6) melalui serangga (kutu daun), dan (7) tanaman krisan sehat (kontrol). Hasil penelitian menunjukkan bahwa tanaman krisan yang diberi vaksin pada berbagai cara aplikasi tidak menunjukkan gejala mosaik. Warna bunga pada semua perlakuan tidak menampakkan gejala pecah warna.Cross protection for controling cucumber mosaic virus on chrysanthemum. One of the virus that attack chrysanthemum is CMV. The alternative of CMV control on plant is the use of vaccine CARNA 5. The objective of the experiment was to find out the application technique of vaccine for CMV protection on chrysanthemum. The experiment was conducted in Virology Laboratory of Indonesia Ornamental Plant Research Institute in Segunung from January to December 2001. RCBD with eight treatments and three replications were used. The treatments were grafting; mechanical inoculation of CARNA 5 with concentration 5 μg/ml, 10 μg/ml, 15 μg/ml, 20 μg/ml, and 25 μg/ml; through vector (aphid), and healthy chrysanthemum plant (control). The results of the experiment showed that chrysanthemum treated with various vaccine application techniques did not show mosaic symptoms. The quality of flower color showed that all treatments did not cause color breaking.
Studi Pembuatan Antiserum Poliklonal untuk Deteksi Cepat Virus Mosaik Mentimun pada Krisan Rahardjo, Indiarto Budi; Muharam, Agus; Sulyo, Yoyo
Jurnal Hortikultura Vol 15, No 2 (2005): Juni 2005
Publisher : Indonesian Center for Horticultural Research and Development

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Virus mosaik mentimun merupakan salah satu patogen penting pada berbagai tanaman hortikultura, termasuk tanaman krisan. Untuk mengetahui secara dini infeksi virus pada tanaman, maka perlu dikembangkan metode deteksi cepat. Penelitian ini bertujuan mendapatkan antiserum poliklonal untuk deteksi cepat virus mosaik mentimun pada krisan. Penelitian dilaksanakan di Laboratorium Virologi Balai Penelitian Tanaman Hias, Segunung dari bulan April sampai Desember 2000. Antiserum terhadap CMV pada tanaman krisan telah dihasilkan dengan cara penyuntikan virus murni CMV pada kelinci dengan konsentrasi setiap penyuntikan sebesar 1 mg/ml. Antiserum yang diuji terdiri dari enam periode pengambilan darah. Pengujian menggunakan metode ELISA tidak langsung. Hasil pengujian menunjukkan bahwa dari enam kali pengambilan darah ternyata bereaksi positif, yaitu dengan adanya konsentrasi antibodi dalam darah meningkat. Antiserum juga dapat digunakan untuk mendeteksi langsung terhadap ekstrak daun krisan yang terinfeksi CMV. Kepekaan antiserum tertinggi pada pengambilan darah ke empat dan ke enam dengan konsentrasi 1/100 dan 1/500 terhadap pengenceran sampel 1/10 dan 1/100. Study on developing of polyclonal antiserum for rapid detection of cucumber mosaic virus on chrysanthemum. Cucumber mosaic virus is one the major pathogens on some horticulture crops, including chrysanthemum. A rapid detection method should be developed to support the evaluation of initial infection of the virus in plants. The objective of this experiment was to obtain polyclonal antiserum to CMV on chrysanthemum for rapid detection. The experiment was conducted in Virology Laboratory of Indonesian Ornamental Plants Institute in Segunung from April to December 2000. A polyclonal CMV antiserum had been produced by injections of purified CMV into rabbits with concentration 1 mg/ml each injections. The antiserum from six bleeding periods were tested. An indirect ELISA method was used to determine the sensitivity of the antiserum. Results indicated that six bleeding periods had positive reaction, with concentration of the virus antibodies increased gradually from the first to the sixth bleedings. The antiserum can also be directly used to detect CMV from infected chrysanthemum plants. The highest antiserum sensitivity were the fourth and sixth bleedings with concentration 1/100 and 1/500 to sample dilution 1/10 and 1/100.
Co-Authors - Hardiyanto - Subhan Adha, Anisa Mutiara Agus Ruswandi Ardiyanto, Eko Astri W Wulandari Atep Sujana Ati Srie Duriat Ayu Wandira Budi Marwoto Budiana, Deden Redy Cahya, Otong E Sukara Hanudin - Hersanti - Hikamudin, Moch. Irfan Hilmi Ridwan Kahmir IM Sudiana Indiarto Budi Rahardjo Maulana, Agis Neni Gunaeni P. M. Winarno Putriyani, Fenny Rahman Rahman Rosyidi, Ahmad S Widawati Suliasih Suliasih Sulistyaningsih, Sri Wahyu Sopandi Wahyuningsih, Liana Wina Mustikaati Winarni, Neni Wiwin Setiawati Yoyo Sulyo