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Keragaman Morfologi dan Genetik Padi Gogo Lokal Asal Banten Mulyaningsih, Enung Sri; Indrayani, Sri
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Superior upland rice cultivar can be assembled through crossbreeding using germplasm diversity which serves as a sourceof gene. Germplasm may constitute of landrace and local cultivars. Banten is one of the provinces that has various localupland rice varieties. Baduy tribe, in the province has played a major role in preserving local upland rice varieties anduphold the wisdom.Genetic diversity information and the benefits of local cultivars can be useful to maximize the selectionprocess of parental candidate for creating new cultivars. This study aims to determine the genetic diversity of local uplandrice from Banten. Methods of analysis diversity performed by phenotypic and genotypic. Observations phenotypic throughobservation of agronomic characters and yield of each cultivar. Whereas genotypic observations conducted with RAPD(random amplified polymorphism DNA). In this study we used 17 local upland rice cultivars from Banten with 14 randomprimers for RAPD. The result showed a mean plant height local upland rice cultivar of Banten was higher than nationalupland rice varieties however productive tillers and rice productivity were lower than national varieties. Nonetheless, localcultivars persisted in this environment because it has the advantage that the community needed. Based on the time ofharvest, local cultivar belonged to early and medium maturing group. Qualitative trait observation showed that there werediversity of stem, leaves, grain and rice among the cultivars. Genetic analysis with RAPD showed nine primers out of 14were successful in amplifying loci from DNA template. The primers having high amplification product were OPJ 05, OPJ07 and OPD07 but the highest polymorphism presentation was OPJ 01. Based on phylogenetic tree, there were two mainclusters. Padi Koneng and Carogol were similar. Three glutinous rice cultivars (Mayang, Jalupang, and Hideung) were inone cluster except Kentan Simpai.Keywords: upland rice, Banten, RAPD, Phenotipe, genotype

Eksplorasi Marka SSR Terpaut Sifat Toleransi Padi Gogo terhadap Alumunium Anggraheni, Yuliana Galih Dyan; Mulyaningsih, Enung Sri
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3099

ABSTRACTThe objective of this research was to study molecular markers were linked to upland rice tolerance of Situ Patenggang x B11930F-TB2 against Al using SSR method. The plant materials were 36 lines of Situ Patenggang x B11930F-TB2 with categories tolerant, moderate, and susceptible to acid soil based on selection in East Lampung. IR6008032 line and ITA variety used as comparative control of tolerant and susceptible. The results showed that 40 primer pairs were applied to 36 lines and allegedly 3 primer pairs that are: RM205, RM257 and RM247 linked to Al tolerance. The markers location analysis on rice chromosome listed as follows: RM205 and RM257 were located on chromosome 9 and RM247 located on chromosome 12 with genetic distance 93,12 cM, 772.62 cM and 13,05 cM, respectively.Keywords: SSR marker, upland rice, aluminium tolerance, ultisols

PEWARISAN GEN PENANDA HPT(HYGROMYCINE PHOSPHOTRANSFERASE) BERDASARKAN ANALISIS PCR DAN EKSPRESINYA PADA POPULASI PADI TRANSFORMAN MENGOVEREKSPRESIKAN GEN HD ZIP OSHOX-6 [Segregation of hpt Gene by PCR Analysis and its Expression in Transgenic Rice Population Overexpressing HD-Zip oshox6 Gene] Mulyaningsih, Enung Sri; Aswidinnoor, Hajrial; Sopandie, Didy; OuwerkerV, Pieter BF; Slamet Loedin, Inez Hortense
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2051

First generation (TO) transgenic plants do not always segregate their transgenes in a Mendellian segregation pattern. Moreover,instability of heterologous gene expression was often observed in transgenic plants. This phenomenon is often called gene silencing. Gene silencing could happen on different level of gene expression, notably at transcriptional or post-transcriptional level.The purpose of this research was to identify the transgene segregation pattern of a marker gene (hpt) as well as the introduced- regulator gene (OsLEA-oshox6) in second generation (Tl) transgenic rice plants.Gene segregation (hpt) analysis was carried out using PCR method. Gene expression analysis was done by hygromycin antibiotic resistant test of leaf samples.Analysis was carried out on 17 lines of Tl transgenic rice plants from Batutegi and Kasalath cultivars.Mendellian segregation pattern of 3:1 was revealed for all lines based on PCR analysis. Gene expression analysis showed almost all lines was segregated in a Mendellian fashion except for Tl-BT III 2C line. Less transgenic plants that expressed the hpt gene were suggested due to gene silencing effects.It was suggested to happen at transcriptional level.

Characterization of Carambola (Averrhoa carambola L.) Plant Collection of Cibinong Plant Germplasm Garden Based on Phenotypic and Genetic Characters Priadi, Dody; Perdani, Ambar Yuswi; Sulistyowati, Yuli; Pohan, Fiqolbi Nuro; Mulyaningsih, Enung Sri
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 1 (2016): March 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i1.5199

Indonesia as a rich biodiversity country has many superior fruit plant germplasms such as sweet star fruit or carambola (Averrhoa carambola L.). Some varieties of carambola which collected at the Germplasm Garden of Research Center for Biotechnology-LIPI have been used for parent trees of fruit plant production. Therefore, they have to be characterized both phenotypically and genetically. The objective of the study was to analyze the relationship between eight varieties of carambola i.e. Malaysia, Penang, Rawasari, Bangkok, Sembiring, Dewabaru, Demak and Dewimurni at the germplasm garden based on phenotypic and genetic characters. Phenotypic characters were observed directly in the field, whereas genetic characters were observed with RAPD markers using 10 primers. Phylogenetic analysis was done using NT-SYS software showed that there were three clusters of carambola varieties. Meanwhile, Malaysia and Penang varieties have closed relationships (96%) compared with the other varieties. The result of the study would be dedicated to updating and completing the existing fruit plant collection database of Plants Germplasm Garden.

Phenotypic Screening of Ds transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayantie, Dwi; Pantouw, Carla F.; Astuti, Dwi; Indrayani, Sri; Rahmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/56

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice, Â combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller. Â Â Keywords: rice, insertion mutant, gene discovery, drought, salinity

Effects of Compost Type and Rootstock Length on Fruit and Vegetable Seedlings Growth in the Nursery Priadi, Dody; Mulyaningsih, Enung Sri
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 3 (2016): December 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

The study was conducted to develop local fruit plants and to improve vegetable production at the Plant Germplasm Garden of RC for Biotechnology-LIPI. Carambola (Averrhoa carambola), durian (Durio zibethinus) and guava (Psidium guajava) were propagated vegetatively (grafting and budding) and were grown on the media containing grass compost (K-1), spent compost of paddy straw mushroom (K-2) or oyster mushroom (K-3) in combination with rootstock length of 45-55 cm (TB-1), 65-75 cm (TB-2) and 75-90 cm (TB-3). Tomato (Lycopersicum esculentum) and kangkung (Ipomoea reptans) were grown on the same media in the screen house. The highest survival rate of grafted durian (71.56%) was obtained from TB-3 grown on K-1 by budding technique. Meanwhile, the highest survival rate of carambola (68.89%) was obtained from TB-1 by grafting technique. The budding technique was not appropriate for guava (0 % of survival). Application of K-3 of 3 kgs on tomato plants resulted in the highest fresh weight, length, and diameter of the fruit, and the highest of plant height, total leaves, and biomass of kangkung. The study is expected to be applied to improve fruit plant growth and survival rate as well as a high production of organic vegetable.How to CitePriadi, D., & Mulyaningsih, E. S. (2016). Effects of Compost Type and Rootstock Length on Fruit and Vegetable Seedlings Growth in the Nursery. Biosaintifika: Journal of Biology & Biology Education, 8(3), 301-307.

The Effects of Fertilizer Treatment, Rhizome Seed Size, and Day of Harvest In Java Turmeric (Curcuma xanthorrhiza Roxb.) Adi, Eko Binnaryo Mei; Mulyaningsih, Enung Sri
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 3 No 2 (2019): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Java turmeric (Curcuma xanthorrhiza Roxb.) is cultivated as a secondary crop, resulting in variable rhizome quality which can be increased by suitable cultivation methods. This study investigated the effect of different cultivation methods on the rhizome yield of Java turmeric. Different fertilizer treatments(none, organic, inorganic, and semi-organic fertilizer), three groups of rhizome seed size (small (50â€“80 g), medium (100â€“150 g), and large (200â€“250 g)) and three groups of harvesting age (eight, ten, and twelve months after planting) were evaluated in a split plot design experiment. Results show that large rhizome seed size together with organic fertilizer treatment increased secondary rhizome production, yielding the highest number, weight and diameter. As high levels of starch in the primary rhizome are crucial for growth of the plant, the use of large rhizomes for propagation is indicated in Java turmeric cultivation. The highest weight and number of primary rhizomes were yielded when plants were harvested twelve months after planting.

USE OF GRASS AND SPENT MUSHROOM COMPOST AS A GROWING MEDIUM OF LOCAL TOMATO (Lycopersicon esculentum Miller) SEEDLING IN THE NURSERY Priadi, Dody; Arfani, Agus; Saskiawan, Iwan; Mulyaningsih, Enung Sri
AGRIVITA, Journal of Agricultural Science Vol 38, No 3 (2016): OCTOBER
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v38i3.671

The objective of this study was to investigate the response of local tomato (Lycopersicon esculentum Miller) seedlings growth on media containing grass and spent mushroom compost in the nursery. The grass compost (GC) was produced by Research Center for Biotechnology-LIPI. Whereas the spent oyster mushroom (Pleurotus ostreatus) compost (OC) and spent paddy straw mushroom (Volvariella volvacea) compost (PC) were produced by Research Center for Biology-LIPI. Growing media of tomato seedling was a mixture ofÂ top soil (TS), rice husk charcoal (HC), grass compost (GC), spent oyster mushroom compost (OC) and spent paddy straw mushroom compost (PC) in the ratio of 1:1 as follows:Â TS+HC, TS+HC+GC, TS+HC+OC and TS+HC+PC. The experiments were arranged in Completely Randomized Design with three replications. Growth parameters observed i.e. seedling growth, dry weight production and leaf indices. Results showed that growing medium containing spent paddy straw mushroom compost was the best to improve seedlings growth in general. However, the lowest specific leaf area (SLA) and leaf area ratio (LAR) indices were obtained by the seedlings on the medium without compost. Correlation analysis showed that highest correlations were recorded for the TS+HC+OC medium. The tomato seedling growth was improved on the media containing compost in general.

PREFERENSI PETANI TERHADAP 15 GALUR PADI GOGO DITABALONG-KALIMANTAN SELATAN Perdani, Ambar Yuswi; Mulyaningsih, Enung Sri; Sulistyowati, Yuli; Suherman, Suherman; Sanjaya, Irwan; Prastyo, Muhammad
Jurnal Agrotek Tropika Vol 8, No 1 (2020): Jurnal Agrotek Tropika Vol 8, Januari 2020
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Upaya menghasilkan suatu varietas unggul baru tanaman padi, sudah selayaknya melibatkan calon pengguna seperti petani. Selera petani atau masyarakat di setiap daerah terhadap suatu varietas dapat berbeda bergantung pada pola konsumsi, budaya dan lingkungan setempat. Penelitian ini bertujuan untuk mengetahui preferensi petani terhadap 15 galur harapan padi gogo toleran aluminium. Pengumpulan data dilakukan di Desa Bintang Ara, Kecamatan Bintang Ara, Kabupaten Tabalong, Kalimantan Selatan. Sebanyak 30 responden petani menilai penampilan galur padi gogo pada fase menjelang panen. Pengamatan dilakukan secara visual terhadap karakter agronomis yang tampak. Sejumlah 15 galur harapan padi gogo ditanam pada petak berukuran 1 m x 5 m dengan jarak tanam 20 cm x 20 cm tanpa rancangan percobaan. Selama proses penanaman terdapat sejumlah kendala antara lain tingkat kesuburan tanah rendah dan serangan hama penyakit tanaman. Namun kendala ini tidak mengganggu produksi dan pengamatan. Hasil penjajakan preferensi petani Bintang Ara terpilih 5 nomor galur harapan padi gogo yaitu: 1, 2, 4, 22, dan 23, Galur-galur tersebut dipilih karena memiliki karakteristik sesuai keinginan calon pengguna. Beberapa karakter utama yang dipilih antara lain: tanaman tinggi, sudut daun bendera lebar, malai panjang, umur tanaman dengan kategori sedang. Sementara karakter unggul lainnya yang disukai ialah jumlah anakan produktif banyak, padi mudah rontok, bulir ramping, warna gabah kuning emas, dan aroma beras wangi.

KERAGAMAN MORFOLOGI DAN GENETIK PADI GOGO LOKAL ASAL BANTEN Mulyaningsih, Enung Sri; Indrayani, Sri
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.337

Superior upland rice cultivar can be assembled through crossbreeding using germplasm diversity which serves as a sourceof gene. Germplasm may constitute of landrace and local cultivars. Banten is one of the provinces that has various localupland rice varieties. Baduy tribe, in the province has played a major role in preserving local upland rice varieties anduphold the wisdom.Genetic diversity information and the benefits of local cultivars can be useful to maximize the selectionprocess of parental candidate for creating new cultivars. This study aims to determine the genetic diversity of local uplandrice from Banten. Methods of analysis diversity performed by phenotypic and genotypic. Observations phenotypic throughobservation of agronomic characters and yield of each cultivar. Whereas genotypic observations conducted with RAPD(random amplified polymorphism DNA). In this study we used 17 local upland rice cultivars from Banten with 14 randomprimers for RAPD. The result showed a mean plant height local upland rice cultivar of Banten was higher than nationalupland rice varieties however productive tillers and rice productivity were lower than national varieties. Nonetheless, localcultivars persisted in this environment because it has the advantage that the community needed. Based on the time ofharvest, local cultivar belonged to early and medium maturing group. Qualitative trait observation showed that there werediversity of stem, leaves, grain and rice among the cultivars. Genetic analysis with RAPD showed nine primers out of 14were successful in amplifying loci from DNA template. The primers having high amplification product were OPJ 05, OPJ07 and OPD07 but the highest polymorphism presentation was OPJ 01. Based on phylogenetic tree, there were two mainclusters. Padi Koneng and Carogol were similar. Three glutinous rice cultivars (Mayang, Jalupang, and Hideung) were inone cluster except Kentan Simpai.Keywords: upland rice, Banten, RAPD, Phenotipe, genotype

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