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Produksi dan Karakterisasi Xilanase dari Isolat Bakteri M-13.2a Asal Air Laut Manado Fawzya, Yusro Nuri; Mangunwardoyo, Wibowo; Munifah, Ifah; Patantis, Gintung
Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 8, No 1 (2013): Juni 2013
Publisher : Balai Besar Riset Pengolahan Produk dan Bioteknologi Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/jpbkp.v8i1.53

Abstract

Isolat bakteri M-13.2A yang berasal dari laut Manado diketahui mampu menghasilkan enzim selulase dan xilanase, berdasarkan pembentukan zona bening pada media padat. Penelitian ini bertujuan untuk mendapatkan informasi lebih lanjut mengenai produksi dan sifat enzim xilanase yang dihasilkan dari isolat bakteri M-13.2A serta identifikasi isolat bakteri tersebut di atas. Sebanyak (2,4-3,3) x 108 cfu/ml inokulum dengan konsentrasi sekitar 9% (v/v) diinokulasikan dalam medium xylan broth, kemudian diinkubasi selama 6 hari pada suhu 30°C, 150 rpm. Pengambilan sampel dilakukan setiap hari dan enzim yang dihasilkan diuji aktivitasnya dengan metode asam dinitro salisilat (DNS). Hasil penelitian menunjukkan bahwa aktivitas xilanase tertinggi dihasilkan pada hari ke-2 inkubasi, sebesar 5,17 U/ml. Enzim xilanase ini bekerja optimum pada pH 8, suhu 70°C. Penambahan ion logam 10 mM memberikan pengaruh yang bervariasi terhadap aktivitas enzim. Ion Zn2+ meningkatkan aktivitas xilanase hingga 278,1%. Ion Fe3+ dan Ca2+ menurunkan aktivitas xilanase menjadi 75 dan 8,3% relatif terhadap kontrol, sedangkan ion K+ tidak memberikan pengaruh terhadap aktivitas xilanase. Hasil identifikasi bakteri menunjukkan bahwa isolat M-13.2A memiliki kemiripan 99% dengan Acinetobacter baumannii.
Antioxidant and Ace Inhibitor Potential of Stripe Trevally Fish (Selaroides leptolepis) Hydrolysate Reinal Putalan; Ifah Munifah; Tati Nurhayati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.319

Abstract

This study aimed to investigate the potency of fish protein hydrolysates (FPH) of stripe trevally fish (Selaroides leptolepis) as antioxidant and ACE inhibitor. The FPH was produced through enzymatically hydrolysis using protease produced by Bacillus licheniformis, a collection of Research and Development Center for Marine and Fisheries Product Processing and Biotechnology (RDCMFPPB). The FPH was fractionated using ultrafiltration membranes with molecular weight cut off (MWCO) of 10, 5 and 3 kDa. The hydrolysis degree, protein content, peptide content, antioxidant activity and Angiotensin-converting enzyme (ACE) inhibitor were observed. The result showed that FPH had maximum hydrolysis degree of 63.91% reached after 6 hours hydrolysis with protein content of 27.43 mg/mL and peptide content of 223.32 mg/mL. That FPH showed antioxidant activity (IC50) of 1941.06 ppm and ACE inhibitor of 87.82% at test concentration of 10 mg/mL. In the ultrafiltration step, the higher molecular cut off used, the higher protein content and peptide content. The lower molecular weight of the hydrolysate, the better antioxidant  and ACE inhibitor activity. The best fractionation that produce potential product to be used as anti-hypertension was in mixture peptides between 3-5 kDa. The IC50 antioxidant activity was 1336.96 ppm and percentage of ACE inhibitor was 97.15% % (with a concentration of 10 mg/mL). From the results, protein hydrolysate of stripe trevally fish produced by enzymatic hydrolysis using local protease was potential as a functional ingredient, particularly as antihypertensive agent.
Evaluation of Antibacterial Assays for Screening of Marine Invertebrate Extracts Nurrahmi Dewi Fajarningsih; Ifah Munifah; Dewi Seswita Zilda
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.294

Abstract

Marine environment continuously produces pharmacologically active compounds. To screen marine natural products as a source of potential antibiotics, it is very important to select an accurate and efficient antibacterial assay. This study aims to find out the best assay for screening of antibacterial activity of marine invertebrate extracts by comparing the performance of 3 methods, i.e. the colorimetric resazurin microtiter assay (REMA), disc diffusion assay and spectrophotometric microdilution assay (SMA). Five marine invertebrate extracts, i.e. Stylissa sp., Theonella sp., Lobophytum sp., Sarcophyton sp., and Aaptos sp., were tested using those 3 different methods. The best method obtained was then further tested for its performance to screen 126 marine invertebrate extracts against 2 bacterial strains i.e. Escherichia coli (ATCC®25922TM) and Staphylococcus aureus (ATCC®25923TM). The study showed that, resazurin microtiter assay (REMA) was simpler, produced quicker result and able to generate reliable data for antimicrobial activity screening compared to the other methods. Moreover, REMA assay is regarded as a suitable assay platform to be implemented for screening of marine invertebrate extracts antibacterial activity since it requires only a small amount of extracts. Based on the MIC values, amongst 126 marine invertebrate extracts screened, 59; 36; 25 and 6 extracts tested against E. coli and 69; 36; 18; and 3 extracts tested against S. aureus were respectively categorized as not active, moderately potential, potential and very potential sample worth to be analyzed further. The ability of the REMA to generate accurate MIC value, which is comparable to the existing antibiotic drug MIC value, will empower researchers to decide whether the extracts worth to be examined further or not. 
Produksi dan Karakterisasi Enzim Selulase Ekstrak Kasar dari Bakteri yang Diisolasi dari Limbah Rumput Laut Isna Rahma Dini; Ifah Munifah
Jurnal Teknologi dan Industri Pertanian Indonesia Vol 6, No 3 (2014): Vol.(6) No.3, October 2014
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (617.634 KB) | DOI: 10.17969/jtipi.v6i3.2315

Abstract

Seaweed waste is a source of bacteria that can produce cellulase enzyme. PMP 0126w isolate is one collection isolate of BBP4BKP obtained from seaweed Glacilaria sp. waste from Pameungpeuk area, Garut, West Java. The aims of this research were to produce and characterize the cellulase enzyme. PMP 0126w isolate was a Gram-positive rod shape bacteria. The isolate had 0.8 cellulolytic index on Carboxymethyl Cellulose (CMC) agar medium. The highest cellulase activity obtained on the third day of fermentation time with a cellulase activity of 0,074 U/mL and specific activity of 0.092 U/mg. Optimum activity of the cellulase enzyme crude extract was pH 5 and 300C. The activity of the cellulase was increased by addition of 5 mM CaCl2 and 10 mM FeCl3 ions and decreased by the addition of 10 mM ZnCl2 ions. The cellulase with the highest activity of 0.237 U/mL was obtained from Glacilaria sp. seaweed waste treated with NaOH 6%. Keywords: cellulose, characterization, production, seaweed waste
Astaxanthin: Senyawa Antioksidan Karoten Bersumber dari Biota Laut Ifah Munifah; Thamrin Wikanta
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 1 (2006): December 2006
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v1i1.71

Abstract

Meskipun berbagai jenis antioksidan dalam makanan telah berkontribusi dalam pencegahan penyakit, namun sejumlah riset yang telah dilakukan lebih memfokuskan pada tiga jenis antioksidan yakni vitamin E, vitamin C dan karotenoid karena berperan penting sebagai nutrien yang diperlukan dalam metabolisme tubuh manusia.