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Journal : Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology

Antioxidant and Ace Inhibitor Potential of Stripe Trevally Fish (Selaroides leptolepis) Hydrolysate Reinal Putalan; Ifah Munifah; Tati Nurhayati; Ekowati Chasanah
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.319

Abstract

This study aimed to investigate the potency of fish protein hydrolysates (FPH) of stripe trevally fish (Selaroides leptolepis) as antioxidant and ACE inhibitor. The FPH was produced through enzymatically hydrolysis using protease produced by Bacillus licheniformis, a collection of Research and Development Center for Marine and Fisheries Product Processing and Biotechnology (RDCMFPPB). The FPH was fractionated using ultrafiltration membranes with molecular weight cut off (MWCO) of 10, 5 and 3 kDa. The hydrolysis degree, protein content, peptide content, antioxidant activity and Angiotensin-converting enzyme (ACE) inhibitor were observed. The result showed that FPH had maximum hydrolysis degree of 63.91% reached after 6 hours hydrolysis with protein content of 27.43 mg/mL and peptide content of 223.32 mg/mL. That FPH showed antioxidant activity (IC50) of 1941.06 ppm and ACE inhibitor of 87.82% at test concentration of 10 mg/mL. In the ultrafiltration step, the higher molecular cut off used, the higher protein content and peptide content. The lower molecular weight of the hydrolysate, the better antioxidant  and ACE inhibitor activity. The best fractionation that produce potential product to be used as anti-hypertension was in mixture peptides between 3-5 kDa. The IC50 antioxidant activity was 1336.96 ppm and percentage of ACE inhibitor was 97.15% % (with a concentration of 10 mg/mL). From the results, protein hydrolysate of stripe trevally fish produced by enzymatic hydrolysis using local protease was potential as a functional ingredient, particularly as antihypertensive agent.
Evaluation of Antibacterial Assays for Screening of Marine Invertebrate Extracts Nurrahmi Dewi Fajarningsih; Ifah Munifah; Dewi Seswita Zilda
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 13, No 1 (2018): May 2018
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v13i1.294

Abstract

Marine environment continuously produces pharmacologically active compounds. To screen marine natural products as a source of potential antibiotics, it is very important to select an accurate and efficient antibacterial assay. This study aims to find out the best assay for screening of antibacterial activity of marine invertebrate extracts by comparing the performance of 3 methods, i.e. the colorimetric resazurin microtiter assay (REMA), disc diffusion assay and spectrophotometric microdilution assay (SMA). Five marine invertebrate extracts, i.e. Stylissa sp., Theonella sp., Lobophytum sp., Sarcophyton sp., and Aaptos sp., were tested using those 3 different methods. The best method obtained was then further tested for its performance to screen 126 marine invertebrate extracts against 2 bacterial strains i.e. Escherichia coli (ATCC®25922TM) and Staphylococcus aureus (ATCC®25923TM). The study showed that, resazurin microtiter assay (REMA) was simpler, produced quicker result and able to generate reliable data for antimicrobial activity screening compared to the other methods. Moreover, REMA assay is regarded as a suitable assay platform to be implemented for screening of marine invertebrate extracts antibacterial activity since it requires only a small amount of extracts. Based on the MIC values, amongst 126 marine invertebrate extracts screened, 59; 36; 25 and 6 extracts tested against E. coli and 69; 36; 18; and 3 extracts tested against S. aureus were respectively categorized as not active, moderately potential, potential and very potential sample worth to be analyzed further. The ability of the REMA to generate accurate MIC value, which is comparable to the existing antibiotic drug MIC value, will empower researchers to decide whether the extracts worth to be examined further or not. 
Astaxanthin: Senyawa Antioksidan Karoten Bersumber dari Biota Laut Ifah Munifah; Thamrin Wikanta
Squalen, Buletin Pascapanen dan Bioteknologi Kelautan dan Perikanan Vol 1, No 1 (2006): December 2006
Publisher : Research and Development Center for Marine and Fisheries Product Processing and Biotechnol

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/squalen.v1i1.71

Abstract

Meskipun berbagai jenis antioksidan dalam makanan telah berkontribusi dalam pencegahan penyakit, namun sejumlah riset yang telah dilakukan lebih memfokuskan pada tiga jenis antioksidan yakni vitamin E, vitamin C dan karotenoid karena berperan penting sebagai nutrien yang diperlukan dalam metabolisme tubuh manusia.