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All Journal HAYATI Journal of Biosciences JBIO: jurnal biosains (the journal of biosciences) Saintia Biologi Microbiology Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Abdimas Talenta : Jurnal Pengabdian Kepada Masyarakat Jurnal Pendidikan Kimia (JPKim) Indonesian Journal of Chemistry Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Serambi Engineering Sarwahita : Jurnal Pengabdian Kepada Masyarakat Jurnal Jejaring Matematika dan Sains Community Service Seminar and Community Engagement (COSECANT)
Erman Munir
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Sumatera Utara
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Physics Social Sciences Other

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ASAI ISOLAT BAKTERI KITINOLITIK BACILLUS SP. BK17 PADA MEDIA PEMBAWA TANAH GAMBUT DAN KOMPOS JANJANG KELAPA SAWIT DALAM MENGHAMBAT PERTUMBUHAN JAMUR PATOGEN SCLEROTIUM ROLFSII DAN FUSARIUM OXYSPORUM PADA KECAMBAH CABAI Hutauruk, Deswidya; Suryanto, Dwi; Munir, Erman
JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Vol 16, No 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.152 KB) | DOI: 10.23960/j.hptt.11661-70

Abstract

Assay of chitinolytic bacterial isolate of Bacillus sp. BK17 in peat and palm oil bunch compost as carrier media in inhibiting Sclerotium rolfsii and Fusarium oxysporum of chilli seedlings. Sclerotium rolfsii and Fusarium oxysporum have been known as causal agents of seedling-off of chilli. Biological control has been used as an alternative control to replace chemical control. This study was aimed to determine the viability and ability of chitinolityc bacteria Bacillus sp. BK17 in carrier media of peat and palm oil bunch compost and in growing media to control seedling-off caused by S. rolfsii dan F. oxysporum of chilli. Our previous study showed that Bacillus sp. BK17 could reduce disease severity and intensity. Bacterial viability was measured as colony number grown after 90 days of storage in minimum salt medium with colloidal chitin as sole C source. Reduction of disease infection was measured as seedling number infected by S. rolfsii dan F. oxysporum. Seedling performances were measured as seedling height, leaf number and dry-weight after 30-days of growth. The result showed that bacterial cell viability was still high in both peat and palm oil bunch compost both with and without colloidal chitin addition after 90 days of storage. It was also shown that during application bacterial cell could grow. Seedling performaces i.e. seedling height, leaf number and dry-weight showed to be normal or even increase compared to those of pathogenic fungal inoculation only and (-) control.
Ulitization of Oil Palm Empty Bunches Waste as a Growth Media for Straw Mushrooms Hastuti, Liana Dwi Sri; Munir, Erman; Tanjung, Rendra Syahputra; Ningrum, Iradani Yupita
ABDIMAS TALENTA: Jurnal Pengabdian Kepada Masyarakat Vol. 6 No. 1 (2021): ABDIMAS TALENTA : Jurnal Pengabdian Kepada Masyarakat
Publisher : Talenta Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (208.462 KB) | DOI: 10.32734/abdimastalenta.v6i1.5803

Abstract

Straw Mushroom (Volvariella volvaceae) as well asmushrooms edible otherhave been developed in Indonesia, including North Sumatra as a substitute for animal protein. Mushrooms or also known as button mushrooms are vegetables that are recognized to have high nutritional value, and are even believed to have medicinal properties for various types of diseases so that they have a fairly high selling value. Some studies report that in 100 grams of mushroom, only 0.17% fat is contained and even better, this fat is not bad fat. Seeing the high demand because of its nutritional value, and its rich content, its taste that is liked by many people, mushroom cultivation has a very promising market value. Basically until now the demand for mushroom continues to increase, but farmers have not been able to meet market needs. good quality start to be limited. Some farmers have started to switch to alternative planting media which is currently mostly practiced, namely oil palm pulp or empty palm oil bunches (TKKS). EFB is a waste that is very easy and is found mostly around palm oil processing factories, whose use has been limited so far as heating materials for boilers and particle wood, many empty bunches have not been utilized. This service aims to provide training in the form of mushroom cultivation using TKKS, as well as provide training in the form of post-harvest handling which is important given the lack of understanding of farmers in production and marketing activities.
Control of Fusarium Wilt of Chili With Chitinolytic Bacteria Dwi Suryanto; Siti Patotah; Erman Munir
HAYATI Journal of Biosciences Vol. 17 No. 1 (2010): March 2010
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.73 KB) | DOI: 10.4308/hjb.17.1.5

Abstract

Biological control of plant disease using antagonistic microorganism has been obtaining much attention and implemented for decades. One of the potential microorganisms used in this strategy is chitinolytic bacteria. Utilization of this bacteria ranges from cell life, enzymes, genes, or other metabolites. In this study, we examined the ability of chitinolytic bacteria as a biocontrol agent of Fusarium wilt of red chili (Capsicum annuum L.) seedlings. The ability of chitinolytic bacteria to suppress the disease was evaluated by soaking red chili seeds in the bacterial isolates solution for 30 minutes prior seedling. Percentage of seedling of treated chili seed at end of study (4-weeks) ranging from 46 to 82.14%. Relative reduction of the seedling damping-off was observed in all bacterial treatment ranged from 28.57 to 60.71%. Furthermore, manifestation of bacterial suppression to Fusarium wilt was also exhibited by increasing of seedling height (ranged from 7.33 to 7.87 cm compared to 6.88 cm) and dry-weight (ranged from 2.7 to 4.3 mg compared to 2.3 mg). However, no significant effect was observed in leaf number. Then, from all chitinolytic isolates tested, BK08 was the most potential candidate for biological control agent of Fusarium wilt in chili seedling.
Isolasi Bakteri Penghasil Biosurfaktan Menggunakan Media yang Mengandung Pestisida Karbosulfan: Isolation of Biosurfactant-Producing Bacteria Using Medium Containing Carbosulfan Pesticide Frans Grovy Naibaho; Nunuk Priyani; Erman Munir; Nina Septania Damanik
Jurnal Jejaring Matematika dan Sains Vol. 2 No. 1 (2020): Edisi Oktober 2020
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Palangka Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36873/jjms.2020.v2.i1.345

Abstract

Penggunaan pestisida sintetik yang tidak terkontrol dapat menyebabkan pencemaran lingkungan karena meningggalkan senyawa pestisida di tanah dan perairan. Bioremediasi adalah salah satu cara untuk mengurangi cemaran senyawa kimia berbahaya di lingkungan dengan menggunakan mikroorganisme. Tujuan penelitian ini ialah untuk memperoleh isolat bakteri potensial penghasil biosurfaktan dari laut Belawan Sumatera Utara dan untuk mengetahui aktivitas biosurfaktannya. Isolasi bakteri dilakukan dengan menggunakan media selektif Bushnell-Hass Agar (BHA) yang mengandung 12 ppm pestisida berbahan aktif karbosulfan sebagai sumber karbon. Aktivitas biosurfaktan dilakukan dengan metode Drop collapsing test dan konsentrasi biosurfaktan dilakukan dengan metose orsinol yang dimodifikasi. Hasil penelitian menunjukkan 9 isolat berhasil diisolasi dan mampu tumbuh pada media yang mengandung karbosulfan. Aktivitas biosurfaktan tertinggi dihasilkan oleh isolat FB6 yang ditandai dengan pembentukan emulsi sebesar 5,627 mL dan produksi biosurfaktan tertinggi ditunjukkan oleh isolat FB7 dengan konsentrasi 54,6 ppm.
ISOLASI DAN UJI ANTIFUNGAL EKSTRAK METANOL, ETIL ASETAT DAN N-HEKSANA BAKTERI ENDOFIT DARI AKAR TUMBUHAN MENTIGI (Vaccinium varingaefolium) Widya Lestari; Dwi Suryanto; Erman Munir
JBIO: jurnal biosains (the journal of biosciences) Vol 3, No 3 (2017): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v3i3.9794

Abstract

Many study that endophytic bacteria useful for plant by protection mechanism to parasitic disease and recovery plant morphology using metabolite producing. Many plants such as mentigi grow at extreme area in volcanic mount. This plant can be able to has specific metabolite producing endophytic bacteria. Research about plant endophytic at extreme area are still limited. The aim of this research to know plant endophytic frommentigi root in inhibiting pathogen fungi growing. plantendophytic bacteria isolation from mentigi root was done by root surface sterilization, cutting and cultivating on nutrient agar medium. Cell extraction was done using methanol, etil-asetat and n-heksana for two isolates. Antagonis test isolates and cell extract was done to some plant and fish pathogen fungus such as Fusarium oxysporum, Ganoderma boninense, Rigidoporus microporusand Saprolegniasp. on potatoes dextrose agar contain 1% yeast extract. Isolates AW5 and AW6 have high potential in inhibiting plant pathogen fungi growing.Essay result extraction to plant pathogen fungi showed that methanol extract of isolate AW5 and AW6 have biggest potential in inhibiting pathogen fungi growing.
PENGURANGAN AKUMULASI TIMBAL (Pb) DENGAN MEMANFAATKAN MIKORIZA ARBUSKULA DAN TANAMAN BELIMBING WULUH (Averrhoa bilimbi) JABON (Anthocephalus cadamba) PETAI (Parkia speciosa) Herna Sianipar; Erman Munir; Delvian Delvian
JBIO: jurnal biosains (the journal of biosciences) Vol 2, No 3 (2016): Jurnal Biosains
Publisher : Universitas Negeri Medan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24114/jbio.v2i3.4955

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh pemberian fungi mikoriza arbuskula (fma)terhadap akumulasi logam Pb tanaman belimbing wuluh, jabon, dan petai, mengetahui dosis pemberian fungi mikoriza arbuskula (fma) yang optimalterhadap akumulasi logam Pb, mengetahui jenis tanaman yang paling efektif dalam akumulasi logam Pb. Penelitian ini dilakukan di di Rumah Kasa, Laboratorium Biologi Tanah dan Laboratorium Sentral Fakultas Pertanian Universitas Sumatera Utara. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) faktorial dengan dua faktor. Faktor pertama adalah inokulasi mikoriza dengan 4 taraf dosis (0 g/bibit, 10  g/bibit, 20 g/bibit, 30 g/bibit) dan faktor kedua adalah tanaman yang terdiri dari tiga jenis tanaman yaitu belimbing wuluh, jabon, dan petai. Hasil penelitian menunjukkan fungi mikoriza arbuskula memberikan  pengaruh terhadap akumulasi logam Pb pada semua tanaman. Dosis mikoriza 10 g optimalterhadap akumulasi logam Pb pada tanaman uji, tanaman jabon yang paling efektif dalam menyerap logam Pb. Rasio akumulasi logam Pb pada bagian akar tanaman  paling tinggi pada tanaman belimbing wuluh yaitu 52,38 mg/kg, rasio akumulasi logam Pb pada bagian batang  tanaman  paling tinggi pada tanaman jabon yaitu 32,7 mg/kg, rasio akumulasi logam Pb pada bagian daun tanaman  paling tinggi pada tanaman jabon yaitu 16,49 mg/kg. Efisiensi penyerapan Pb tanaman  paling tinggi pada tanaman jabon yaitu 13,06 % dan terendah pada belimbing wuluh  yaitu 8,6 % Kata Kunci : Fungi Mikoriza Arbuskula, Logam Berat Pb, Belimbing Wuluh, Jabon , Petai.
ISOLASI DAN UJI POTENSI BAKTERI TANAH PERTANIAN BERASTAGI SUMATERA UTARA DALAM MENDEGRADASI FUNGISIDA ANTRACOL BERBAHAN AKTIF PROPINEB Diah Sri Utami; Nunuk Priyani; Erman Munir
Saintia Biologi Vol 1, No 2 (2013)
Publisher : Saintia Biologi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.671 KB)

Abstract

Isolation and potential Berastagi agricultural soil bacteria, North Sumatra in degrading propineb-based antracol fungicide has been conducted. The isolated bacteria were grown on Bushnell Hass Broth (BHB) containing 2% of propineb-based antracol fungicide with propineb as the active compound. The cultures have been grown on shaking incubator at 150 rpm for 21 days. The media BHB containing 2% of propineb-based antracol fungicide without bacteria was used as a control. The parameters observed were the growth bacterial, biosurfactant activity, biosurfactant concentration and the residues of propineb which were observed on day 0th, 7th, 14th and 21th. A total of sixteen bacterial isolates were isolated using selective media Bushnel Hass Agar (BHA) containing 2% propineb-based antracol fungicide​​. Two bacterial isolates which were CBA 02 and JBA 04 were selected for further test to determine their ability to degrade propineb. JBA 04 showed much higher ability in reducing propineb concentration up to 60.86%, while CBA 02 was only 5.59% than that of control.   Keywords: biodegradation, bioremediation, biosurfactant, fungicides, propineb
Isolation and Characterization of Chitinolytic Bacteria and Their Potential to Inhibit Plant Pathogenic Fungi DWI SURYANTO; NETTI IRAWATI; ERMAN MUNIR
Microbiology Indonesia Vol. 5 No. 3 (2011): September 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (66.653 KB) | DOI: 10.5454/mi.5.3.8

Abstract

A study on the isolation and characterization of chitinolytic bacteria and their potential to inhibit plant pathogenic fungi has been done. The bacteria were isolated from the soil of Karo, Langkat, and Bangka, Sumatra. Ganoderma boninense, Fusarium oxysporum, and Penicillium citrinum of the stock cultures in Laboratory of Microbiology Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara were used for growth inhibition assay by the isolated bacteria. KR05 and LK08 shared similar morphological and physiological characters; like wise, KR07 shared property similarities with BK08. All bacterial isolates inhibited the growth of G. boninense F. oxysporum , and P. citrinum at a different extent. LK08 showed the highest inhibition rate followed by BK07 and BK09. However, P. citrinum was inhibited more by BK07 and BK09. The crude enzyme preparation of the latter isolate exhibited the highest chitinase activity. The result suggested that their swarming activity seemed to contributed to inhibition of fungal growth.
KEMAMPUAN BAKTERI ENDOFIT TANAMAN SEMANGKA DALAM MENEKAN PERKEMBANGAN PENYAKIT BERCAK DAUN YANG DISEBABKAN OLEH JAMUR COLLETOTRICHUM SP. Eryna Elfasari Rangkuti; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (408.67 KB) | DOI: 10.23960/j.hptt.214170-177

Abstract

Ability of watermelon endophytic bacteria to suppress development of leaf spot disease caused by Colletotrichum sp. A studi on assay of endophytic bacteria to control Colletotrichum sp., causal agent of leaf spot disease on watermelon, was conducted. Colletotrichum sp. was isolated from infected leaf of leaf spot disease, while endophytic bacteria were isolated from stem, leaf, and root of watermelon healthy plant. Antagonistic assay was conducted by dual culture method. Hyphal abnormalities as a result of antagonistic assay was observed using light microscope. To determine the ability of endophytic bacterial isolates to control leaf spot disease, watermelon seeds were treated by dipping the seed in endophytic bacterial suspension. Seven endophytic bacterial isolates showed to inhibit Colletotrichum sp. to some extent. Two isolates DS 01 and BS 01 showed relatively high inhibition zone compared to others, therefore were choosen for further study. Abnormal hyphae such as broken, lysis, twisted, curled, and swollen hyphae were recorded as the result of antagonistic assay. Watermelon seed treatment revealed that dipping the seed in endophytic bacterial suspension of DS 01 and BS 01 reduced leaf spot disease to 12 and 24%, but inhibited seed growth by 12% and 44%, respectively. It seemed that all treatments showed to reduce seedling performance i.e seedling height, leaf number, and dry weight, compared to that of (-) control. DS 01 however seemed to increase dry weight of watermelon plants.
ASAI KEMAMPUAN BAKTERI ENDOFIT DARI KACANG TANAH DALAM MENGHAMBAT PERTUMBUHAN Sclerotium sp. PADA KECAMBAH KACANG TANAH Lisa Novita Arios; Dwi Suryanto .; Kiki Nurtjahja .; Erman Munir .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (886.632 KB) | DOI: 10.23960/j.hptt.214178-186

Abstract

Assay on ability of endophytic bacteria isolated from peanut to inhibit Sclerotium sp. growth in peanut seedlings. A study on assay of ability of endophytic bacteria to inhibit Sclerotium sp. in peanut seedling has been done. The bacteria were isolated from peanut healthy plants, while Sclerotium sp. was isolated from infected peanaut plant. Antagonistic assay was conducted by dual culture method. In vivo assay of inhibiting Sclerotium sp. was conducted by dipping peanut seed in bacterial solution, and planting the seed in soil:compost (3:1) growing media. Six endophytic bacterial isolates showed to inhibit the growth of Sclerotium sp. in vitro. LN1 seemed to inhibit more of Sclerotium sp., while LN5 showed to inhibit less. Two potential isolates LN1 of gram-negative and LN2 of gram-positive using for further study showed to decrease more of dumping off. It also seemed that the isolates increased the seedling height, number of leaves, and dry weight.
Co-Authors . Elvitriana Apri Heri Iswanto Arbi Maulana Arida Susilowati Arif Nuryawan Bejo Slamet Delvian Delvian Deni Elfiati Deswidya Hutauruk, Deswidya Diah Sri Utami DWI SURYANTO Dwi Suryanto Edi Munawar Eryna Elfasari Rangkuti Fachri Fauzi Frans Grovy Naibaho Hamonangan Nainggolan Harisyah Manurung Harry Agusnar Herna Sianipar Hesti Wahyuningsih Irvan Irvan It Jamilah Iwan Risnasari Risnasari KIKI NURTJAHJA Liana Dwi Sri Hastuti Liana Dwi Sri Hastuti, Liana Dwi Sri Lisa Novita Arios Mohammad Basyuni Muhammad Nizar N S Sepwin NETTI IRAWATI Nin Suharti Nina Septania Damanik Ningrum, Iradani Yupita Nunuk Priyani Nunuk Priyani Siti Patotah Tanjung, Rendra Syahputra Taufik Ashar Widya Lestari Wirsal Hasan