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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia ACTA VETERINARIA INDONESIANA Hemera Zoa Forum Pasca Sarjana Jurnal Sain Veteriner Buletin Peternakan Jurnal Veteriner Jurnal Agripet Media Penelitian dan Pengembangan Kesehatan Jurnal Ilmu Ternak dan Veteriner Jurnal Ilmu dan Teknologi Peternakan Tropis Jurnal Riset Veteriner Indonesia (Journal of The Indonesian Veterinary Research) ELSE (Elementary School Education Journal) : Jurnal Pendidikan dan Pembelajaran Sekolah Dasar Jurnal Agroqua: Media Informasi Agronomi dan Budidaya Perairan Indonesian Aquaculture Journal Jurnal Riset Akuakultur ARSHI Veterinary Letters JURNAL Al-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI Indonesian Journal of Tropical Aquatic JURNAL AKTUAL AKUNTANSI KEUANGAN BISNIS TERAPAN (AKUNBISNIS) JURNAL PENGABDIAN PENDIDIKAN MASYARAKAT (JPPM) Prosiding Seminar Nasional Unimus

sri murtini .

Departemen Ilmu Penyakit Hewan Dan Kesehatan Masyarakat Veteriner, Fakultas Kedokteran Hewan Institut Pertanian Bogor

Author-ID : 325742

Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Library & Information Science Mathematics Nursing Public Health Social Sciences Veterinary Other

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A study of inoculation route and dosage levels on embryonated chicken eggs as media for testing tea mistlestoe (Scurrula oortiana) extract activity Murtini, Sri; Murwarni, R; Satrija, F; Malole, M.B.M
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Tea mistlestoe extract (Scurrula oortiana) has cytotoxic activity which is potential to be used in preventing viral induced-chicken tumor. The following study was designed to evaluate the effects of different inoculation routes, dosage levels, and strains of embryonated chicken eggs as media for testing the tea mistlestoe extract (Scurrula oortiana) antiviral activity. Proper inoculation route was examined by inoculation of the extract at dose level of 0,2 mg/egg into embryonated layer eggs via allantoic cavity, chorio-allantoic membrane, and yolk sac. Effect of dose level of tea mistlestoe extract on embryo development was examined in groups of embryonated broiler eggs inoculated with the extract at 0.02, 0.2, 2, 20, or 200 mg/egg. Inoculation of tea mistlestoe extract into allantoic cavity was the safest procedure as indicated by the absence of embryos mortality, and faster embryo growth compared to those of chorio-allantoic membrane and yolk sac-inoculated eggs. The extract induced different growth effects when inoculated into embryonated layer or broiler eggs. Administration of the extract at dose levels between 0,02â200 mg/egg reduced significantly the weight of broiler embryoes, but not the relative weights of liver, heart and spleen. Administration of similar dosage in layer embryoes did not cause any significant difference in the embryoes weight. This study suggests that the study of antiviral activity of tea mistlestoe extract in embryonated chicken eggs should be carried out on embryonated eggs of layer breeds and the extract should be inoculated via allantoic cavity. Key Words: Scurrula oortiana, Embryonated Chicken Eggs, Enti Viral Activity

DETEKSI ANTIGEN EKSKRETORI-SEKRETORI Schistosoma japonicum DENGAN METODE ELISA PADA PENDERITA SCHSISTOSOMIASIS DI NAPU SULAWESI TENGAH Samarang, Samarang; Satrija, Fadjar; Murtini, Sri; Nurjana, Made Agus; Chadijah, Sitti; Maksud, Malonda; Tolistiawaty, Intan
Media Penelitian dan Pengembangan Kesehatan Vol 25, No 1 Mar (2015)
Publisher : Badan Penelitian dan Pengembangan Kesehatan

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bstrakDeteksi antigen ekskretori-sekretori Schistosoma japonicum (S.japonicum) dengan metode ELISA pada penderita schistosomiasis dilakukan di Napu Kabupaten Poso selama sembilan bulan, yaitu dari April hingga Desember 2013. Tujuan dari penelitian ini adalah mendapatkan nilai optical density (OD)pada penderita positif schistosomiasis dengan infeksi tinggi, sedang, dan rendah. Menetapkan nilai sensitivitas dan spesifiitas dari konformasi ELISA yang digunakan. Kegiatan dalam penelitian yang dilakukan meliputi kegiatan di lapangan dan kegiatan di laboratorium. Kegiatan di lapangan antara lainsurvei tinja dan survei darah. Kegiatan di labotarorium adalah optimasi ELISA. Hasil penelitian yaitu diperoleh nilai absorbansi pada infeksi rendah berkisar 0.468 Â± 0.699 dengan kepadatan telur 1-10 telur/ slide, pada infeksi sedang nilai absorbansinya berkisar 0.700 Â± 0.899 dengan kepadatan telur 11-20telur/slide dan untuk infeksi tinggi nilai absorbansinya yaitu 0.900 Â± 1.166 dengan kepadatan telur 21-44 telur/slide. Nilai sensitivitas sebesar 74% dan untuk nilai spesifiitasnya sebesar 90%. Kesimpulan dari penelitian ini adalah metode ELISA yang dikembangkan mempunyai nilai sensitivitas dan spesifiitasbaik untuk mendeteksi AgES S. japonicum pada serum penderita schistosomiasis.Kata Kunci: Schistosomiasis, ELISA, Sensitivitas, Spesifiitas, Indonesia.AbstractDetection of Schistosomajaponicum (S.japonicum) excretory-secretory antigens by ELISA method in human schistosomiasiswas conducted in Poso district Napu valey for nine months, from April to December 2013. The purpose of the study was to get the optical density for the low, medium, and high infection at human schistosomiasis and than to determine the specifiity and sensitivity ELISA conformation. The activities in this study with the laboratory and the fild. The fild activities included stool survey and blood survey. The laboratory activities was optimization of the ELISA method. The results of the study obtained value of sensitivity was 74% and specifiity 90%. Absorbance values ranges from 0699Â±0468 with density of eggs 1-10 eggs/slide was low infection, the absorbance values was 0.700Â±0.899 for medium infection the density of eggs 11-20 eggs/slide and high infection the absorbance values were 0.900Â±1,166 with density of eggs 21-44 eggs/slide. Therefore, it can be concluded of this study that developed ELISA method has good sensitivity and specifiity values for detecting ESAg S.japonicumin human schistosomiasis.Keywords: Schistosomiasis, ELISA, sensitivity, specifiity, Indonesia

Behavior, Histopathology and Physiological Responses of Rat Fed Diets Containing Growth Hormone Transgenic Fish Meal Alimuddin Alimuddin; Sri Murtini; Nurly Faridah; Sri Nuryati
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.

Serologi dan virologi virus avian influenza H5N1 pada kucing jalanan di Kota Bogor Sri Murtini; R. Susanti; Ekowati Handharyani
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Highly pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen in birds. Recently, the virus has been reported to cause sporadic fatal disease in tigers, leopards, and other exotic felids as well as domestic cats in Thailand. The present study was carried out to investigate the presence of AI H5N1 virus infection in stray cats roaming around residential, traditional and chicken farms in Bogor, West Java. Ninety serum samples were tested using HI test to screened for the presence of antibody to AI H5N1. Virus isolation was done in SPF embrionated chicken eggs and identify using HI, AGP and RT-PCR. The results showed that 18,9% of stray cats developed antibodies against H5 with geometric mean titre 23,1 . Stray cats lived in traditional markets 18–40% developed antibodies in the titre ranging from 22,8 to 24,5. Only two out of nine stray cats which lived in chicken farm developed low antibody titres again H5 (21). None of the stray cats lived in residencial area have developed antibodies against H5. This study revealed that stray cats have been contact with AI H5. Avian influenza H5 viruses were isolated in eight out of 33 pooled of rectal swab samples. The viral cleavage site sequences are CCTCAAAGAGAGAGC AGAAGAAAGAAGAGAGGT which represent amino acid sequences of PQRESRRKKRG. Based on the cleavage site sequence, the isolates are similar with the AI H5 virus subtype isolated from human in Indonesia during 2005–2007.

PEMBUATAN DAN STANDARISASI ANTIGEN AI H5N1 KOMERSIAL UNTUK MONITORING TITER ANTIBODI HASIL VAKSINASI AI DI INDUSTRI PETERNAKAN AYAM Retno D. Soejoedono; Sri Murtini; Kamalludin Zarkasie
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Vaccination is one of the chosen strategy for controling AI H5N1 in Indonesia. Vaccination able to induce protective antibodies against AI but unable to inhibit viral infection. Determination of antibody titers in the serum from bird vaccinated with AI-H5N1 vaccine consisting of 2 or 3 different AI virus isolates difficult to be meassured if the antigen for HI test is uncalibrated yet. Furthermore, the determination of a minimum protective antibody titer against the challenge of AI virus circulating in the field at this time needs to be done. This study aims to determine the H5N1 AI virus antigen for standart HI test and the minimum titre of antibodies that able neutralize virus infection. As much as 55 chickens were divided into 11 groups, 10 groups vaccinated with commercial AI vaccine and AI H5N1 field isolat antigen. Four types of commercial vaccines were veccinated to one group and seven other groups vaccinated with the antigen AI Legok 2004, Nagrak Ag 2009, Ag Lawang 2010, as well as polyvalent Ag combination of these three types of antigen. After third vaccinations, the presence of antibodieswere meassured by HI test. Serum with a titer test 26-28 were tested for the capability of virus neutralizationin using virus neutralization test against three different H5N1 AI virus field isolates. The test results showed that the H5N1 subtype AI virus antigen representative as standart antigen for HI test is antigen Legok 2004 and the minimum titer which able neutralize H5N1 AI virus field isolates 28.

Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini; Bayu Febram; Retno Wulansari; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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The objective of this experiment was to detect antibody (IgG) against enterotoxigenic Escherichia coli (ETEC)K99 in the blood of cows vaccinated by Escherichia coli polyvalent vaccine. Eight dry cows were injectedsubcutaneously by polyvalent Escherichia coli twice prior to parturition. Before vaccinated, the cows were givenimmunomodulator orally for 3 days. Blood samples were drawn from coccigeal vein prior to the 1st vaccination,two week following the 1st vaccination and at 1, 2, and 4 weeks after the 2nd vaccination. Blood samples wereanalyzed for IgG and ETEC K99 using indirect Enzyme Linked Immunosorbent Assay (ELISA) assay. Results of theexperiment indicated that absorbance values of all vaccinated cows before the first vaccination until third weekfollowing the 2nd vaccination were below cut off values. The absorbance values then increased and were above cutoff values at fourth week following the 2nd vaccination. In conclusion, antibody against ETEC K99, were detected inthe blood of cows, fourth week following the 2nd vaccination.

Seroprevalensi dan Faktor Risiko Rabies pada Anjing di Daerah Endemis Kabupaten Sukabumi Ardilasunu Wicaksono; Abdul Zahid; Etih Sudarnika; Dordia Anindita Rotinsulu; Denny Widaya Lukman; Sri Murtini; Yusuf Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.436

Sukabumi District is reported to date as an endemic rabies area in West Java Province. In supporting the rabies control programs, the occurrence of rabies needs to be determined to decrease the number of cases. This research aims to determine the seroprevalence of rabies and identify the risk factors associated with rabies in the district. The research was conducted in Jampang Tengah and Cisolok sub-districts with positive rabies cases on dogs and humans. The blood samples were taken from 191 selected dogs using simple random sampling. Rabies antibody titer was measured using ELISA, and the risk factor was identified using selected dogs’ data classified into intrinsic and extrinsic characteristics. Data were analyzed using the chi-square test and the calculation of the odds ratio. The results showed that the seroprevalence of rabies was 7.9% (4.9-10.9%). The average antibody titer was 0.23 EU with a minimum value of 0.03 EU and a maximum of 0.96 EU. Thus, as high as 7.8% of the population showed rabies seropositivity from the unvaccinated dogs, while among the vaccinated dogs, only 8.0% of the population with rabies protective antibody titer. The significant risk factor of rabies seroprevalence is the typology of raising area (p = 0.003), revealing that the dogs raised in the coastal area had the odds of 5.8 (1.6-21.2) higher to get the rabies seropositivity than the dogs raised in the highland area. Keywords: antibody titre, dogs, rabies, risk factor, seroprevalence

Kinerja Kesehatan Sapi Neonatus yang Diberi Kolostrum dari Induk Sapi yang Divaksin Escherichia coli Retno Wulansari; Anita Esfandiari; I Wayan Teguh Wibawan; Sri Murtini
Acta VETERINARIA Indonesiana Vol. 4 No. 1 (2016): Januari 2016
Publisher : IPB University

Penelitian ini dilakukan untuk mempelajari status kesehatan anak sapi baru lahir yang diberi kolostrum berasal dari induk yang telah divaksinasi dangan vaksin E. coli in-aktif. Sebelas ekor anak sapi baru lahir digunakan dalam penelitian ini dibagi menjadi dua kelompok yaitu kelompok kolostrum (diberi kolostrum sapi) dan non-kolostrum (diberi susu sapi). Kolostrum atau susu sapi diberikan sebanyak 10% berat badan secara langsung setelah dilahirkan dan selanjutnya tiap 12 jam selama 3 hari. Uji tantang dilakukan dengan pemberian E. Coli K99 hidup peroral saat anak sapi berumur 14 jam. Pemeriksaan status kesehatan termasuk suhu tubuh, frekuensi pulsus dan napas serta kualitas defikasi pada 0,12, 24, 48, 72 dan 168 jam setelah uji tantang. Hasil penelitian ini memperlihatkan bahwa diarrhea timbul pada 12 sampai 26 jam setelah uji tantang pada semua anak sapi kelompok non-kolostrun dan beberapa anak sapi kelompok kolostrum. Kelompok non-kolostrum memperlihatkan tanda klinis dengan diarrhe parah sebagai diarrhe profus yang berwarna pucat kekuningan. Bahkan 1 ekor mati pada 3 hari setelah uji tantang. Sebaliknya pada kelompok kolostrum hanya memperlihatkan diarrhe sedang pada 5 dari 8 ekor. Kesimpulan dalam penelitian ini adalah konsumsi kolostrum yang berasal dari induk yang telah divaksinasi dengan E. Coli memperlihatkan sifat protektif terhadap infeksi E. coli K99.Kata kunci: Escherichia coli, kolostrum, anak sapi baru lahir. (The Health Performance of Neonatal Calves Received Colostrum from Cows Vaccinated by Escherichia coli)This experiment was conducted to study the health status of neonatal calves received colostrum from cows vaccinated by in-active E. coli. Eleven healthy newborn calves were used in this experiment and divided into two groups, i.e colostrum (received bovine colostrum) and non-colostrum group (received bovine milk). Colostrum or milk were given to the calves at 10% of body weight directly after birth and followed every 12 h, for three days. Challenges were done orally to all newborn calves when they were 14 hours of ages, used live E. coli K-99. Examination of health status included body temperature, pulses and respiration rates, and defecations quality at 0, 12, 24, 48, 72 and 168 hours after challenges. Results of the experiment showed that diarrhea appeared at 12-26 hours after challenges in all calves of non-colostrum group and part of calves in colostrum group. The non-colostrum group showed a severe clinical signs of diarrhea as watery profuse diarrhea with pale yellowish color. One calf even death at three days after challenges. In contrast, the colostrum group showed only a mild diarrhea in 5 out of 8 calves. In conclusion, the consumption of colostrum originated from cows vaccinated by E. coli showed protective properties against E. coli K-99 infection.Keywords: Escherichia coli, colostrum, neonatal calves

Prevalensi Sistiserkosis pada Babi Hutan (Sus scrofa) yang dipotong di Tempat Pemotongan Hewan (TPH) Bengkulu Tengah, Bengkulu Noviriliensi Hartika; Elok Budi Retnani; Sri Murtini
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Sistiserkosis/taeniasis merupakan penyakit zoonosis terabaikan yang memiliki dampak serius bagi ekonomi dan kesehatan masyarakat. Tujuan dari penelitian ini adalah untuk menentukan prevalensi sistiserkosis pada babi hutan di Bengkulu Tengah, Bengkulu. Penelitian ini dilakukan di tempat pemotongan hewan Bengkulu Tengah, Provinsi Bengkulu Indonesia antara Februari-Mei 2016. Delapan puluh dua ekor babi hutan di koleksi serum dari vena jugularis dan post-mortem karkasnya. Pemeriksaan post-mortem meliputi otot lidah, trisep, bisep, masseter, diafragma, intercostae, jantung, dan pemeriksaan hati untuk menentukan adanya sistiserkus. Serum di pisahkan dari darah masing-masing sampel dan di uji sirkulasi antigen dari sistiserkus menggunakan monoklonal antibodi-sandwich enzyme-linked immunosorbent assay (MOAB-ELISA). Hasil pemeriksaan post-mortem tidak di temukan adanya sistiserkus. Hasil uji ELISA didapatkan 8 sampel (9,8%) terdeteksi adanya antigen sistiserkus. Babi hutan dengan hasil seropositif sistiserkus ditemukan berasal dari kecamatan dengan prevalensi tertinggi kecamatan Pagar Jati (39,0%) diikuti oleh kecamatan Bang Haji (30,5%) dan prevalensi terendah ditemukan di kecamatan Pematang Tiga (30,5%). Penelitian ini menyatakan bahwa terdapat infeksi sistiserkus pada babi hutan dari Bengkulu Tengah, Bengkulu.

Preparasi Imunoglobulin Yolk (IgY) Spesifik Virus Rabies untuk Pengembangan Kit Diagnostik Suwarny Ruhi; Sri Murtini; Okti Nadia Poetri; Retno D Soejoedono
Acta VETERINARIA Indonesiana Vol. 6 No. 1 (2018): Januari 2018
Publisher : IPB University

Penelitian ini bertujuan untuk memproduksi dan mengkarakterisasi IgY anti rabies sebagai bahan diagnostik. Ayam petelur usia produktif divaksinasi dengan vaksin rabies inaktif secara parenteral melalui rute intramuskular dengan dosis 0,5 ml sebanyak 2 kali. Keberadaan IgY pada telur dievaluasi dengan metode ELISA. Konsentrasi total protein IgY di hitung dengan metode Bradford. IgY dipurifikasi menggunakan dua metode yaitu : 1) pengendapan dengan NaCl, PEG 6000-amonium sulfat; 2) teknik Water Soluble Fraction (WSF), dilanjutkan pengendapan dengan PEG 6000-amonium sulfat. Titer IgY spesifik di tentukan dengan uji ELISA dan karakterisasi protein dengan metode SDS-PAGE. Hasil pengujian menunjukkan, antibodi anti-rabies dapat dideteksi pada kuning telur di minggu kedua setelah vaksinasi pertama. Purifikasi IgY dengan NaCl menghasilkan konsentrasi 331 µg/ml dan teknik WSF 184 µg/ml. Karakterisasi protein pada teknik NaCl menghasilkan 6 pita protein dengan berat molekul 164,16 kDa, 126,43 kDa, 97,36 kDa, 68,73 kDa, 40,76 kDa, 28,77 kDa sedangkan teknik WSF hanya terdiri dari 3 pita dengan berat molekul 94,03 kDa, 65,61 kDa, dan 31,94 kDa. Titer antibodi spesifik menggunakan teknik NaCl lebih besar dari 0,5 IU/ml dan teknik WSF dengan titer antibodi di bawah 0,5 IU/ml . Berdasarkan hasil penelitian, dapat disimpulkan bahwa IgY spesifik rabies dapat diproduksi pada ayam petelur dan menghasilkan titer antibodi ≥ 0,5 IU/ml, dengan titer antibodi spesifik rabies sebesar ≥ 0,5 IU/ml.

Co-Authors . Alimuddin A. Wafa Manshur Abdul Zahid Adi Supryatno Agik Suprayogi Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alvianita G. Putri Andrijanto Hauferson Angi Angelina N. Tethool Anita Esfandiari Anna Heirina Annisa Madyanti Geminastiti Parampasi Arif Purwo Mihardi Asah Hilaliah Ayi Santika Bambang Sigit Widodo Bayu Febram Budhi Adhiani Cece Sumantri Ch. Budhi Adhiani Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dwi Hany Yanti Dwi Retno Wulandari Edi Sukmawinata Edi Sukmawinata Eka Murtiasri Eko Budiyanto Ekowati Handharyani Elok Budi Retnani Etih Sudarnika F Satrija Fitria Susanti Ghiandra Naufal Syazily Saukhan I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Joko Pamungkas Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Kenda Adhitya Nugraha Koekoeh Santoso Leni Maylina Lestari, Alif Putra Luci Cyrilla M. Noor Ardiansah M.B.M Malole Made Agus Nurjana Malonda Maksud Marlefzena Marlefzena Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Asrori Muhammad Fikri Al Habib Muhammad Noor Ardiansah Nanis Nurhidayah Neksidin Neksidin Nensy Tri Putri Niken Ulupi Noor Rohman Setiawan Noviriliensi Hartika Noviyanti Noviyanti Nugraha, Arifin Budiman Nugroho Hari Purnomo Nurly Faridah Okti Nadia Poetri Okti Nadia Poetri Purwaningsih Purwaningsih R Murwarni R. Harry Soehartono R. Susanti Raditya Pradana Putra Rani Raharjani Rani Raharjanti Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Riki Siswandi Risqika Akla Velayati Rita Mutia Rotinsulu, Dordia Anindita Rudiansyah Rudiansyah Safitria Wulandari Samarang Samarang Sekar Ayu Chairunnisa Sitti Chadijah Sri Nuryati Sugiarti Sugiarti Sulistinah Sus Derthi Widhyari Sus Derthi Widhyarti Susena Susena Suwarny Ruhi Tarigan, Ronald TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Ulfah Hidayati Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Usamah Afiff Wicaksono, Ardilasunu Wijaya, Agus Wiwien Mukti Andriyani Wiwien Mukti Andriyani Wiwik Sri Utami Yeni Novita Yus Rusila Noor Yusuf Ridwan

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