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Journal : MAKARA of Science Series

Identification of Gene Resistance to Avian Influenza Virus (Mx Gene) among Wild Waterbirds Dewi Elfidasari; Dedy Duryadi Solihin; Retno Damayanti Soejoedono; Sri Murtini
Makara Journal of Science Vol 17, No 1 (2013): April
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

The Mx gene is an antiviral gene used to determine the resistance or the susceptibility to different types of viruses, including the Avian Influenza (AI) virus subtype H5N1. The AI virus subtype H5N1 infection in chickens causes Mx gene polymorphism. The Mx+ gene shows resistant to the AI virus subtype H5N1, whereas the Mx- gene shows signs of susceptible. The objective of this research was to detect the Mx gene in wild aquatic birds using the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) method with the primer pairs F2 and NE-R2/R and the RsaI restriction enzyme. DNA samples were obtained from eight species of wild waterbirds with positive and negative exposure to the AI virus subtype H5N1. DNA amplification results showed that the Mx gene in wild aquatic birds is found in a 100 bp fragment, which is the same as the Mx gene found in chickens. However, unlike chickens, the Mx gene in wild aquatic birds did not show any polymorphism. This study proves that Mx- based resistance to AI virus subtype H5N1 in different in wild birds than in chickens. Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance Keywords: Mx gene, wild waterbirds, exposure, AI virus subtype H5N1, resistance
Serosurveillance of Avian Influenza Virus Subtype H5N1 with Haemagglutination-Inhibition on Wild Aquatic Birds in Pulau Dua Serang Natural Reserves, Banten Province Dewi Elfidasari; Dedy Duryadi Solihin; Retno Damayanti Soejoedono; Sri Murtini; Yus Rusila Noor
Makara Journal of Science Vol 15, No 2 (2011): November
Publisher : Directorate of Research and Community Engagement, Universitas Indonesia

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Abstract

Further detailed research is required to obtain deeper information on the role of wild birds on the distribution of Avian influenza in Asia. A research has been carried out on February–June 2007 focused on blood sampling (serosurveillance) of wild birds in Pulau Dua Nature Reserves (CAPD), Serang, Banten. The research is aimed to investigate the infection of AI virus sub-tye H5N1 on the studied wild birds. The blood samples were taken from studied aquatic birds, followed by HI (haemagglutination-inhibition) test. A total of 183 samples represent 7 water bird species were taken i.e Cattle egret Bubulcus ibis, Javan pond-heron Ardeola speciosa, Little egret Egretta garzetta, Intermediate egret Egretta intermedia, Black-crowned night heron Nycticorax nycticorax, Great egret Casmerodius albus and Grey heron Ardea cinerea. The result revealed that 41 (23.27%) samples showed the present of AIV antibodies serotype H5N1 which is identified as positive. Data showed 5 positive-test species, including B. ibis (29.27%), E. garzetta (29.27%), E. intermedia (4.88%), Ardeola speciosa (7.32%), and N. nycticorax (29.27%). A total of 41.46% were infected adult individual, whereas 58.54% were juveniles.
Co-Authors . Alimuddin Abdul Zahid Adi Supryatno Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Andrijanto Hauferson Angi Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Arif Purwo Mihardi Asah Hilaliah Ayi Santika Bambang Sigit Widodo Bayu Febram Cece Sumantri Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dwi Hany Yanti Edi Sukmawinata Edi Sukmawinata Eko Budiyanto Ekowati Handharyani Elok Budi Retnani Etih Sudarnika F Satrija Fitria Susanti I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Joko Pamungkas Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Kenda Adhitya Nugraha Koekoeh Santoso Leni Maylina Lestari, Alif Putra Luci Cyrilla M.B.M Malole Made Agus Nurjana Malonda Maksud Marlefzena Marlefzena Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Fikri Al Habib Muhammad Yusuf Ridwan Nanis Nurhidayah Niken Ulupi Noor Rohman Setiawan Noviriliensi Hartika Noviyanti Noviyanti Nugraha, Arifin Budiman Nugroho Hari Purnomo Nurly Faridah Okti Nadia Poetri Purwaningsih Purwaningsih R Murwarni R. Susanti Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Risqika Akla Velayati Rita Mutia Rotinsulu, Dordia Anindita Safitria Wulandari Samarang Samarang Sekar Ayu Chairunnisa Sitti Chadijah Sri Nuryati Sulistinah Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Ronald TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tethool, Angelina Novita Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Usamah Afiff Wicaksono, Ardilasunu Wijaya, Agus Wiwien Mukti Andriyani Wiwien Mukti Andriyani Wiwik Sri Utami Yus Rusila Noor Yusuf Ridwan Yusuf Ridwan