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Journal : Jurnal Ilmu Pertanian Indonesia

PEMBUATAN DAN STANDARISASI ANTIGEN AI H5N1 KOMERSIAL UNTUK MONITORING TITER ANTIBODI HASIL VAKSINASI AI DI INDUSTRI PETERNAKAN AYAM Retno D. Soejoedono; Sri Murtini; Kamalludin Zarkasie
Jurnal Ilmu Pertanian Indonesia Vol. 17 No. 1 (2012): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Vaccination is one of the chosen strategy for controling AI H5N1 in Indonesia. Vaccination able to induce protective antibodies against AI but unable to inhibit viral infection. Determination of antibody titers in the serum from bird vaccinated with AI-H5N1 vaccine consisting of 2 or 3 different AI virus isolates difficult to be meassured if the antigen for HI test is uncalibrated yet. Furthermore, the determination of a minimum protective antibody titer against the challenge of AI virus circulating in the field at this time needs to be done.  This study aims to determine the H5N1 AI virus antigen for standart HI test and the minimum titre of antibodies that able neutralize virus infection. As much as 55 chickens were divided into 11 groups, 10 groups vaccinated with commercial AI vaccine and AI H5N1 field isolat antigen. Four types of commercial vaccines were veccinated to one group and seven other groups vaccinated with the antigen AI Legok 2004, Nagrak Ag 2009, Ag Lawang 2010, as well as polyvalent Ag combination of these three types of antigen. After third vaccinations, the presence of antibodieswere meassured by HI test. Serum with a titer test 26-28 were tested for the capability of virus neutralizationin using virus neutralization test against three different H5N1 AI virus field isolates. The test results showed that the H5N1 subtype AI virus antigen representative as standart antigen for HI test is antigen Legok 2004 and the minimum titer which able neutralize H5N1 AI virus field isolates 28.
Respons Antibodi Anti ETEC K99 pada Induk Sapi Bunting Setelah Pemberian Vaksin Escherichia Coli Polivalen Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini; Bayu Febram; Retno Wulansari; Leni Maylina
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 2 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this experiment was to detect antibody (IgG) against enterotoxigenic Escherichia coli (ETEC)K99 in the blood of cows vaccinated by Escherichia coli polyvalent vaccine. Eight dry cows were injectedsubcutaneously by polyvalent Escherichia coli twice prior to parturition. Before vaccinated, the cows were givenimmunomodulator orally for 3 days. Blood samples were drawn from coccigeal vein prior to the 1st vaccination,two week following the 1st vaccination and at 1, 2, and 4 weeks after the 2nd vaccination. Blood samples wereanalyzed for IgG and ETEC K99 using indirect Enzyme Linked Immunosorbent Assay (ELISA) assay. Results of theexperiment indicated that absorbance values of all vaccinated cows before the first vaccination until third weekfollowing the 2nd vaccination were below cut off values. The absorbance values then increased and were above cutoff values at fourth week following the 2nd vaccination. In conclusion, antibody against ETEC K99, were detected inthe blood of cows, fourth week following the 2nd vaccination.
Serologi dan virologi virus avian influenza H5N1 pada kucing jalanan di Kota Bogor Sri Murtini; R. Susanti; Ekowati Handharyani
Jurnal Ilmu Pertanian Indonesia Vol. 14 No. 1 (2009): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Highly  pathogenic avian influenza (HPAI) H5N1 virus is a known pathogen in birds. Recently, the virus has been reported to cause sporadic fatal disease in tigers, leopards, and other exotic felids as well as domestic cats  in Thailand. The present study was carried out to investigate the presence of AI H5N1 virus infection in stray cats roaming around residential, traditional and chicken farms in Bogor, West Java. Ninety serum samples were tested using HI test to screened for the presence of antibody to AI H5N1. Virus isolation was done in SPF embrionated chicken eggs and identify using HI, AGP and RT-PCR. The results showed that 18,9% of stray cats developed antibodies against H5 with geometric mean titre 23,1 . Stray cats lived in traditional markets 18–40% developed antibodies in the titre ranging from 22,8  to 24,5. Only two out of nine stray cats which lived in chicken farm developed low antibody titres again H5 (21). None of the stray cats lived in residencial area have developed antibodies against H5. This study revealed that stray cats have been contact with AI H5. Avian influenza H5 viruses were isolated in eight out of 33 pooled of rectal swab samples. The viral cleavage site sequences are CCTCAAAGAGAGAGC AGAAGAAAGAAGAGAGGT which represent amino acid sequences of PQRESRRKKRG. Based on the cleavage site sequence, the isolates are similar with the AI H5 virus subtype isolated from human in Indonesia during 2005–2007.
Seroprevalensi dan Faktor Risiko Rabies pada Anjing di Daerah Endemis Kabupaten Sukabumi Ardilasunu Wicaksono; Abdul Zahid; Etih Sudarnika; Dordia Anindita Rotinsulu; Denny Widaya Lukman; Sri Murtini; Yusuf Ridwan
Jurnal Ilmu Pertanian Indonesia Vol. 26 No. 3 (2021): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.26.3.436

Abstract

Sukabumi District is reported to date as an endemic rabies area in West Java Province. In supporting the rabies control programs, the occurrence of rabies needs to be determined to decrease the number of cases. This research aims to determine the seroprevalence of rabies and identify the risk factors associated with rabies in the district. The research was conducted in Jampang Tengah and Cisolok sub-districts with positive rabies cases on dogs and humans. The blood samples were taken from 191 selected dogs using simple random sampling. Rabies antibody titer was measured using ELISA, and the risk factor was identified using selected dogs’ data classified into intrinsic and extrinsic characteristics. Data were analyzed using the chi-square test and the calculation of the odds ratio. The results showed that the seroprevalence of rabies was 7.9% (4.9-10.9%). The average antibody titer was 0.23 EU with a minimum value of 0.03 EU and a maximum of 0.96 EU. Thus, as high as 7.8% of the population showed rabies seropositivity from the unvaccinated dogs, while among the vaccinated dogs, only 8.0% of the population with rabies protective antibody titer. The significant risk factor of rabies seroprevalence is the typology of raising area (p = 0.003), revealing that the dogs raised in the coastal area had the odds of 5.8 (1.6-21.2) higher to get the rabies seropositivity than the dogs raised in the highland area. Keywords: antibody titre, dogs, rabies, risk factor, seroprevalence
Co-Authors . Alimuddin Abdul Zahid Adi Supryatno Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Andrijanto Hauferson Angi Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Arif Purwo Mihardi Asah Hilaliah Ayi Santika Bambang Sigit Widodo Bayu Febram Cece Sumantri Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dwi Hany Yanti Edi Sukmawinata Edi Sukmawinata Eko Budiyanto Ekowati Handharyani Elok Budi Retnani Etih Sudarnika F Satrija Fitria Susanti I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Joko Pamungkas Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Kenda Adhitya Nugraha Koekoeh Santoso Leni Maylina Lestari, Alif Putra Luci Cyrilla M.B.M Malole Made Agus Nurjana Malonda Maksud Marlefzena Marlefzena Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Fikri Al Habib Muhammad Yusuf Ridwan Nanis Nurhidayah Niken Ulupi Noor Rohman Setiawan Noviriliensi Hartika Noviyanti Noviyanti Nugraha, Arifin Budiman Nugroho Hari Purnomo Nurly Faridah Okti Nadia Poetri Purwaningsih Purwaningsih R Murwarni R. Susanti Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Risqika Akla Velayati Rita Mutia Rotinsulu, Dordia Anindita Safitria Wulandari Samarang Samarang Sekar Ayu Chairunnisa Sitti Chadijah Sri Nuryati Sulistinah Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Ronald TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tethool, Angelina Novita Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Usamah Afiff Wicaksono, Ardilasunu Wijaya, Agus Wiwien Mukti Andriyani Wiwien Mukti Andriyani Wiwik Sri Utami Yus Rusila Noor Yusuf Ridwan Yusuf Ridwan