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Journal : Jurnal Ilmu Ternak dan Veteriner

A study of inoculation route and dosage levels on embryonated chicken eggs as media for testing tea mistlestoe (Scurrula oortiana) extract activity Murtini, Sri; Murwarni, R; Satrija, F; Malole, M.B.M
Indonesian Journal of Animal and Veterinary Sciences Vol 11, No 2 (2006)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.597 KB) | DOI: 10.14334/jitv.v11i2.518

Abstract

Tea mistlestoe extract (Scurrula oortiana) has cytotoxic activity which is potential to be used in preventing viral induced-chicken tumor. The following study was designed to evaluate the effects of different inoculation routes, dosage levels, and strains of embryonated chicken eggs as media for testing the tea mistlestoe extract (Scurrula oortiana) antiviral activity. Proper inoculation route was examined by inoculation of the extract at dose level of 0,2 mg/egg into embryonated layer eggs via allantoic cavity, chorio-allantoic membrane, and yolk sac. Effect of dose level of tea mistlestoe extract on embryo development was examined in groups of embryonated broiler eggs inoculated with the extract at 0.02, 0.2, 2, 20, or 200 mg/egg. Inoculation of tea mistlestoe extract into allantoic cavity was the safest procedure as indicated by the absence of embryos mortality, and faster embryo growth compared to those of chorio-allantoic membrane and yolk sac-inoculated eggs. The extract induced different growth effects when inoculated into embryonated layer or broiler eggs. Administration of the extract at dose levels between 0,02–200 mg/egg reduced significantly the weight of broiler embryoes, but not the relative weights of liver, heart and spleen. Administration of similar dosage in layer embryoes did not cause any significant difference in the embryoes weight. This study suggests that the study of antiviral activity of tea mistlestoe extract in embryonated chicken eggs should be carried out on embryonated eggs of layer breeds and the extract should be inoculated via allantoic cavity. Key Words: Scurrula oortiana, Embryonated Chicken Eggs, Enti Viral Activity
A study of inoculation route and dosage levels on embryonated chicken eggs as media for testing tea mistlestoe (Scurrula oortiana) extract activity Sri Murtini; R Murwarni; F Satrija; M.B.M Malole
Jurnal Ilmu Ternak dan Veteriner Vol 11, No 2 (2006): JUNE 2006
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (127.597 KB) | DOI: 10.14334/jitv.v11i2.518

Abstract

Tea mistlestoe extract (Scurrula oortiana) has cytotoxic activity which is potential to be used in preventing viral induced-chicken tumor. The following study was designed to evaluate the effects of different inoculation routes, dosage levels, and strains of embryonated chicken eggs as media for testing the tea mistlestoe extract (Scurrula oortiana) antiviral activity. Proper inoculation route was examined by inoculation of the extract at dose level of 0,2 mg/egg into embryonated layer eggs via allantoic cavity, chorio-allantoic membrane, and yolk sac. Effect of dose level of tea mistlestoe extract on embryo development was examined in groups of embryonated broiler eggs inoculated with the extract at 0.02, 0.2, 2, 20, or 200 mg/egg. Inoculation of tea mistlestoe extract into allantoic cavity was the safest procedure as indicated by the absence of embryos mortality, and faster embryo growth compared to those of chorio-allantoic membrane and yolk sac-inoculated eggs. The extract induced different growth effects when inoculated into embryonated layer or broiler eggs. Administration of the extract at dose levels between 0,02–200 mg/egg reduced significantly the weight of broiler embryoes, but not the relative weights of liver, heart and spleen. Administration of similar dosage in layer embryoes did not cause any significant difference in the embryoes weight. This study suggests that the study of antiviral activity of tea mistlestoe extract in embryonated chicken eggs should be carried out on embryonated eggs of layer breeds and the extract should be inoculated via allantoic cavity. Key Words: Scurrula oortiana, Embryonated Chicken Eggs, Enti Viral Activity
Avian Beta Defensin 2 (AvBD2) Gene Polymorphism Identification in IPB-D1 Chicken Masrurah Masrurah; Isyana Khaerunnisa; Sri Murtini; Cece Sumantri
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 2 (2021): June 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i2.2715

Abstract

Avian Beta Defensin 2 (AvBD2) gene, which is located in chromosome 3, plays an important role in the immune system of the chicken by inhibiting the development of microorganisms such as bacteria that infect body tissues. Defensins are produced through epithelial cells immediately after tissue injury or infection, which then processes the maturation of dendritic cells to initiate an immune response in the lymph nodes. The purpose of this study was to discover the polymorphism of the AvBD2 gene in IPB-D1 chickens. PCR and direct-DNA sequencing methods were used to identify the diversity of intron 1, exon 2, and intron 2 AvDB2 genes in 47 chickens. Genotype and allele frequency, and heterozygosity calculations were carried out to obtain information of the AvBD2 gene polymorphism. A total of 10 single nucleotide polymorphisms were found in the AvBD2 gene located in intron 1 (g.4843T>A, g.4853G>A, and g.4859T>C), exon 2 (g.4881A>G, g.4889G>A, and g.5002C>T), and intron 2 (g.5075C>T, g.5111T>G, g.5116G>T, and g.5177G>T). All SNPs are polymorphic. The g.5002C>T mutation causes changes in the amino acid Ala to Val which has the potential to be a candidate for characterizing disease resistance in IPB-D1 chickens.
Co-Authors . Alimuddin Abdul Zahid Adi Supryatno Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Andrijanto Hauferson Angi Anita Esfandiari Annisa Madyanti Geminastiti Parampasi Arif Purwo Mihardi Asah Hilaliah Ayi Santika Bambang Sigit Widodo Bayu Febram Cece Sumantri Daryono Denny Widaya Lukman Denny Widya Hikman DEWI APRI ASTUTI Dewi Elfidasari Dwi Hany Yanti Edi Sukmawinata Edi Sukmawinata Eko Budiyanto Ekowati Handharyani Elok Budi Retnani Etih Sudarnika F Satrija Fitria Susanti I Gusti Ngurah Kade Mahardika I wayan Teguh Wibawan I.W. Teguh Wibawan Intan Tolistiawaty Irma Isnafia Arief Isyana Khaerunnisa Ita Mardiani Zain Joko Pamungkas Kamalludin Zarkasie Kathirina Beatrik Riwu Wolo Kenda Adhitya Nugraha Koekoeh Santoso Leni Maylina Lestari, Alif Putra Luci Cyrilla M.B.M Malole Made Agus Nurjana Malonda Maksud Marlefzena Marlefzena Masrurah Masrurah Melly Pratiwi Setyawati Melly Pratiwi Setyawati Muhammad Fikri Al Habib Muhammad Yusuf Ridwan Nanis Nurhidayah Niken Ulupi Noor Rohman Setiawan Noviriliensi Hartika Noviyanti Noviyanti Nugraha, Arifin Budiman Nugroho Hari Purnomo Nurly Faridah Okti Nadia Poetri Purwaningsih Purwaningsih R Murwarni R. Susanti Retno D Soejoedono Retno D. Soejoedono Retno Damajanti Soejoedono Retno Damayanti Soejoedono Retno Setyaningsih Retno Wijayanti Retno Wulansari Ridi Arif Risqika Akla Velayati Rita Mutia Rotinsulu, Dordia Anindita Safitria Wulandari Samarang Samarang Sekar Ayu Chairunnisa Sitti Chadijah Sri Nuryati Sulistinah Sus Derthi Widhyari Sus Derthi Widhyarti Suwarny Ruhi Tarigan, Ronald TARUNI SRI PRAWAST MIEN KAOMINI ANY ARYANI DEDY DURYADI SOLIHIN Tethool, Angelina Novita Ulfatin Khoiriyah Herowati Upik Kesumawati Hadi Usamah Afiff Wicaksono, Ardilasunu Wijaya, Agus Wiwien Mukti Andriyani Wiwien Mukti Andriyani Wiwik Sri Utami Yus Rusila Noor Yusuf Ridwan Yusuf Ridwan