Kahar Muzakhar
Biology Dept., Faculty of Mathematic and Natural Sciences, The University of Jember, Jl. Kalimantan 37, Jember 68121

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GROWTH PROFILES AND ETHANOL PRODUCTION OF TWO YEAST STRAINS Kluyveromyces marxianus IFO-288 AND Kluyveromyces marxianus IFO-617 DURING FERMENTATION OF SOYBEAN PULP HYDROLYZATE SUBSTRATES Muzakhar, Kahar
International Journal of Biosciences and Biotechnology Vol 1 No 2 (2013)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Two yeast strains, K.marxianus IFO-288 and K.marxianus IFO-617, were observed to utilize carbon andnitrogen sources obtained from enzymatically hydrolyzed soybean pulp (hydrolyzate). Under aerobiccondition where 1.5% hydrolyzate was used, the maximum biomass of the two yeast yield reached 30%with the protein contents of 47-53%. The two yeast strains also fermented the soybean pulp hydrolyzateand produced alcohol when anaerobic condition was set. The alcohol production effi ciencies of the K.marxianus IFO-288 and K. marxianus IFO-617were 49% and 59.7%, respectively in the medium containing1.5% hydrolyzate. These values were noticed to decrease for the two strains when the concentration of thehydrolyzate was increased. At the concentrations of 2.5% and 5% hydrolyzate for examples, the alcoholproduction effi ciencies of the K.marxianus IFO-288 became 42.5% and 18.5%, respectively, while 45.6% and20.8%, respectively were recorded in the medium inoculated with K. marxianus IFO-617. At 10% hydrolyzateconcentration, none of the yeast strains grew or produced alcohol, indicating that some inhibitory substancesin hydrolyzates were present in the fermentation medium.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

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Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
SUGAR PRODUCTION BY DIGESTING OF OIL PALM EMPTY FRUIT BUNCH USING EXTRACELLULAR ENZYMES FROM Aspergillus niger AND Trichoderma reesei FOR ETHANOL PRODUCTION Muzakhar, Kahar; S, Sutoyo; S, Siswoyo
International Journal of Biosciences and Biotechnology Vol 2 No 1 (2014)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Extracellular enzymes which obtained from 4 days cultivation Aspergillus niger and Trichoderma reesei onsolid state fermentation of oil palm empty fruit bunch (OPEFB) were used for lignocellulosic-­??rich OPEFBdigestion. The enzymes were concentrated using 70% saturated ammonium sulphate, dialysed against20mM acetate buffer at pH 5 and adjusted one tenth (v/v) from the initial volume with the same buffer.The concentrated enzymes were then used in hydrolysation of powdered OPEFB. Amount of 10.65 mg/ mland 11.47 mg/ml sugars were produced when each concetrated enzyme A. niger and T. reesei mixedwith2%OPEFB. These hydrolysation were done on 100 ml total volume, incubated at 37oC with 100 rpmshaken for 36 hours. Further, both hydrolyzates results were sterilised and fermented anaerobically usingSaccharomycess cerevisiae at concentration 0.5mg/ml cells and incubated in 30oC for 24 hours. Colorimetricanalysis using QuantiChrom Kit DIET-­??500 at OD 580nm gave results the alcohol production were 0.86%and 0.92% which were similar with Gas Chromatograph analysis that of 0.83% and 0.93%, respectively.
Preliminary Investigation: Stearidonic Acid Production by Genetically Modified Saccharomyces cerreviseae Using Linseed Oil as A Fatty Acid Source Muzakhar, Kahar
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Stearidonic acid (SDA); 18:4(n-3), an ω-3 Polyunsaturated Fatty Acid (PUFA), could be produced by Genetically Modified Saccharomyces cerreviseae (GMY) using Linseed Oil (LO) as fatty acid source. In nature, S. cerrevisae accumulates only very small amount lipid and contains until mono unsaturated fatty acids. But this GMY strain beside has capability to accumulate lipid, it also contains Δ6 desaturase gene which leads to convert α-Linolenic Acid (ALA); 18:3(n-3) to be SDA. Gas Chromatograph analysis of transmethylated LO sample showed that main component of fatty acid was ALA (about 57%), therefore LO can be used as a cheap source for other PUFA production. In order to provide ALA as a source for the yeast, enzymatic hydrolysis of LO using lipase was done. The lipase from Rhizomucor miehei (L4277-SIGMA) showed the highest activity among 5 lipases when 1 g/L LO in the medium for yeast (containing 6.7g/L yeast nitrogen base, 20 g/L glucose and 2.5g/L tergitol NP-40) was hydrolyzed. For SDA production, 1g/L LO in medium was aseptically hydrolyzed by lipase in 50 unit/mL for 18 hours at 30 oC and 140 rpm. The pre-culture of GMY (1% V/V) was then inoculated into the treated medium and 2.59 g/L dried cells were obtained after 5 days cultivation. ALA was accumulated in the cells at 0.06g/L (11% of total ALA in LO), and only 25% (0.015g/L) of accumulated ALA were converted to SDA. These results suggested that LO can be used as a source for PUFA production. In order to improve the productivity of SDA using GMY, hydrolysis of LO as well as cultivation condition and genetically improvement of the yeast must be highly considerated.
Phosphate Solubilizing Bacteria Adaptive to Vinasse Muzakhar, Kahar; Sutoyo, Sutoyo; Saragih, Ahmad Bukhari
Journal of Mathematical and Fundamental Sciences Vol. 47 No. 2 (2015)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2015.47.2.8

Abstract

Microorganisms identified as phosphate solubilizing bacteria (PSB) adaptive to vinasse were successfully screened from sugarcane soil from an agriculatural estate in Jatiroto. By conducting a screening on Pikovskaya's agar medium (PAM), we found that five different isolates were detected as PSB (pvk-5a, pvk-5b, pvk-6b, pvk-7a, and pvk-8a). Of the five isolates only three could be grown and were found to be adaptive to vinasse based medium without any nutrients added (pvk-5a, pvk-5b and pvk-7a). The three isolates were  characterized as coccus and Gram negative with no endospores detected. We suggest that these three isolates can be used as biofertilizer agent to  support organic farming.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Khofiya, Zunairoh Nidaan; Winarsa, Rudju; Muzakhar, Kahar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9915

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml. Kata Kunci: Kulit buah kopi, hidrolisis, produksi PST, Pestalotiopsis sp.
Hidrolis Kulit Buah Kopi Oleh Kapang Pestalotiopsis sp. VM 9 Serta Pemanfaatan Hidrolisatnya Sebagai Medium Produksi Protein Sel Tunggal Saccharomyces cerevisiae Khofiya, Zunairoh Nidaan; Winarsa, Rudju; Muzakhar, Kahar
BERKALA SAINSTEK Vol 7 No 1 (2019)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v7i1.9682

Abstract

Hidrolisis limbah kulit buah kopi oleh enzim ekstraseluler Pestalotiopsis sp. VM 9 telah dilakukan. Hasil hidrolisis menunjukkan bahwa dalam hidrolisat mengandung gula reduksi 392,35μg/ml setelah masa inkubasi hari ke-6. Analisis lanjutan menunjukkan bahwa hidrolisat dapat digunakan sebagai medium pertumbuhan Protein Sel Tunggal (PST) S. cerevisiae dengan tingkat pertumbuhan hingga mencapai kepadatan sel 8,5x10 6 sel/ml selama 72 jam kultivasi. Selama pertumbuhannya S. cerevisiae terbukti mengkonsumsi sumber karbon dari gula reduksi sebanyak 211,91 μg/ml.
Isolation of Genes Encoding Arthropod Odorant Binding Proteins (OBP), D7 from Salivary Gland Vectors of Malaria: Anopheles sundaicus Pawana, Nuryatmaja Gora; Muzakhar, Kahar; Senjarini, Kartika
Jurnal ILMU DASAR Vol 16 No 1 (2015)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v16i1.621

Abstract

The isolation of Arthropod Odorant Binding, D7 protein, encoding genes from Anopheles sundaicus and An. maculatus mosquitos as the malaria vectors in Indonesia is necessary to recognize their characteristic. The isolated genes can be used to develop the Transmission Blocking Vaccine (TBV). This research aims to characterize the D7 protein encoding genes from An. sundaicus and An. maculatus through the synthesis of complementary DNA (cDNA) of D7 protein by using D7 protein primer that has been used for the other species of Anopheles. The mosquitos were taken from Dusun Parasputih, Bangsring, Wongsorejo, Banyuwangi, Jawa Timur. Isolation of the salivary gland was done by performing microdisection method and the isolation of the total RNA was done by performing High Pure RNA Isolation Kit (Roche-Germany). Synthesis of cDNA D7 encoding gene and its amplification were performed by using Maxime RT-PCR Premix Kit (iNtRon Biotechnology). The result of the total RNA and RT-PCR were run  in agarose gel and visualized under the UV transiluminator. Based on the visualization, we found that the salivary gland total RNA of female An. sundaicus was 500-750 base pair (bp). The RT-PCR visualization showed a band sized below 100 bp and it was concluded not to be the size of the D7 protein encoding gene. An incompatibility of D7 primer from An. gambiae with cDNA template from An. sundaicus was suspected to be the reason of the gene isolation failure.Keywords: gene isolation, D7 protein, salivary gland, Anopheles sundaicus
PRODUKSI GUM ARABIC BALURAN SEBAGAI PENDUKUNG PENGEMBANGAN WISATA KAMPUNG BANTENG DI KARANG TEKOK SEBAGAI WILAYAH PENYANGGA TN BALURAN Kusumah, Maulana S.; Wiyono, Hidayat Teguh; Subekti, Agus; Muzakhar, Kahar; Winarsa, Rudju
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol 4 No 1 (2020): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : Dewan Pimpinan Daerah (DPD) Forum Dosen Indonesia JATIM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36339/je.v4i1.272

Abstract

This article is the result of PPDM (Mitra Desa Service Program) about Baluran bos javanicus (Banteng) village tourism. Development of bull village tourism is an effort to solve the problem of wild grazing in Bunaken National Park. In the event of Banteng Village Tourism, it is necessary to support tourism, namely creative industries, agro-tourism, and NTFP production (non-timber forest products). One of the NTFPs that is relied upon is Arabic gum. Currently, cattle breeders in the banteng village area have been able to produce Arabic gum as a result of the introduction of tapping technology by the 2019 PPDM team. The dedication method is in the form of dissemination and field practice. Three groups representing breeders were trained to tap acacia gum through a drilling method combined with ethephon induction as GIS. One week after application, the group begins harvesting gum and submits the results to the group leader. Then the group leader sends the results to the Cooperative in Pondok Pesantren Assalam, Sumberanyar, Banyuputih Situbondo. The amount of Baluran Arabic gum that was collected by the group for three months reached 143.9 kg. This service activity concludes that the strength in producing Baluran Arabic gum is significant in improving the welfare of breeders in supporting the maintenance and retention of a Banteng.