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Rochman Naim

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Author-ID : 327716

Education Medicine & Pharmacology

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Detection of Mycobacterium avium subspecies paratuberculosis in formula milk from Bogor using PCR IS 900 Nugroho, Widagdo S.; Sudarwanto, Mirnawati; Lukman, Denny W.; Naim, Rochman; Hassan, Abdulwahed A.; Usleber, Ewald
Medical Journal of Indonesia Vol 17, No 3 (2008): July-September
Publisher : Faculty of Medicine Universitas Indonesia

Crohnâs disease (CD) that becomes a public health concern in developed countries shows similarities in clinical signs and pathological features with Johneâs disease (JD) in ruminants infected by Mycobacterium avium subspecies paratuberculosis (MAP). Few researches conducted in Europe, the USA, and Australia showed relationships between MAP, CD, JD and dairy products. Indonesians consume milk and diary products from domestic and imported source. Adji in 2004 found some domestic dairy cows that were seropositive for MAP, and this could be a serious problem in dairy farm animals and human health in the future. The aim of this study was to detect MAP in the growing up formula milk. Fifty samples from five established factories were taken from supermarkets in Bogor. Polymerase chain reaction method (PCR) with insertion sequence (IS) 900 as primer and culture in Herroldâs egg yolk media with mycobactin J (HEYM J) as a gold standard were used in this study. Neither MAP grew up in HEYM J medium after 20 weeks of culture period nor positive samples by PCR IS 900 were found. Although there were no positive samples found in this study, further extensive and comprehensive studies on MAP should be done with more and varied samples, as well as in human to provide data on MAP in Indonesia. (Med J Indones 2008; 17: 183-7)Keywords: Crohnâs disease, dairy cow, growing up formula milk

Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy he Widagdo Sri Nugroho; Mirnawati Sudarwanto; Denny Widaya Lukman; Surachmi Setyaningsih; Rochman Naim; Ewald Usleber
Forum Pasca Sarjana Vol. 32 No. 2 (2009): Forum Pascasarjana
Publisher : Forum Pasca Sarjana

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Mycobacterium avium subspecies paratuberculosis (MAP) becomes a public health concern in developed countries which associated with Crohn’s disease (CD) in human and Johne’s disease (JD) in ruminants. Some researchers in Europe, USA, and Australia detected MAP in the dairy products and showed the relationship among MAP, CD, and JD. Meanwhile Indonesia imported milk and milk products from those countries to cover national demand. In the future it will be a potential problem to national dairy herd and human health. The aim of this study is to detect MAP in the growing up milk formula. Fifty samples from five established distributors were taken in Bogor. Some diagnostic methods were used parallel in this study, namely Mycobacterial Growth Indicator Tube (MGIT), Herrold’s Egg Yolk enrichment with mycobactine-J (HEYM) and polymerase chain reaction method (PCR) with insertion sequence IS 900 and F 57 as primer. Neither MAP grew up in MGIT and HEYM after 20 weeks of incubation period. No positive samples were found by conventional PCR using IS 900 and F57 either but 5 samples were detected positive by nested PCR F57. Although there was no evidence of MAP grew from the samples in this study, the comprehensive and sustainable studies on MAP still should be carried out with more and varied samples, as well as in human to provide data on MAP and to anticipate it in Indonesia. Key words: mycobacterium avium subspecies paratuberculosis, growing up milk formula, PCR

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