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Lymphokine activated killer cells from peripheral blood mononuclear cells of endometriosis of patients improve cytotoxicity to endometriosis cell culture Natadisastra, Muharam; Bustami, Arleni A.; Mansur, Indra G.; Jacoeb, Teuku Z.; Giustiniani, Jerome; Schiavon, Valerie; Bensussan, Armand
Medical Journal of Indonesia Vol 20, No 2 (2011): May
Publisher : Faculty of Medicine Universitas Indonesia

Background: To assess the increased cellular immunity of Peripheral Blood Mononuclear Cells (PBMC) derivedÂ LAK cells from endometriosis patients towards endometriosis cell cultures after stimulation with IL-2.Methods: This study is a quasi-experimental study of pre and post treatment using controls. Phenotype evaluation ofÂ CD3+CD4+, CD3+CD8+ and CD56+ effector cells of PBMC from endometriosis patients and controls was performed.Â Cytotoxicity test of PBMC from endometriosis patients and control towards Daudi, K562 cell line and endometriosisÂ cell cultures using 51Chromium release assay was also carried out.Results: Phenotype evaluation of PBMC from endometriosis patients (n = 10) and controls (n = 6) were done prior to andÂ after IL-2 stimulation. Before IL-2 stimulation, CD3+CD4+, CD56+ from endometriosis group (n = 10) tend to be lowerÂ than control (n=6) whereas CD3+CD8+ were higher in endometriosis group than controls. After IL-2 stimulation, CD3+ CD8+, CD56+ of PBMC from endometriosis group were significantly increased (p < 0.05). Cytotoxicity test revealedÂ a significant increase (p < 0.05) in both PBMCâs effector cells from endometriosis and control group towards targetÂ cells, Daudi, and K562 cell lines after IL-2 stimulation. PBMCâs effector cells cytotoxicity from both endometriosisÂ and control towards target endometriosis cell cultures were also elevated after IL-2 stimulation.Conclusion: LAK cells derived IL-2 stimulated PBMC from endometriosis patients increased cellular immunityÂ towards endometriosis cell cultures. (Med J Indones 2011; 20:87-93)Keywords: Daudi cell line, endometriosis, endometriosis cell cultures, K562 cell line, LAK cells, NK cells

Homocysteine Level in the Blood and Follicular Fluid is Higher in Infertile Women with Endometriosis Natadisastra, Muharam
Indonesian Journal of Obstetrics and Gynecology Volume. 37, No. 2, April 2013
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.231 KB)

Objective: To compare and determine the differences in the level of homocysteine in the blood and follicular fluid in infertile women with and without endometriosis, then analyze the effect of homocysteine levels to oocyte quality. Methods: This study was cross-sectional study. Fifty-nine subjects following the in-vitro fertilization program are included in the admission criteria were divided into two equal groups, ie groups of endometriosis and without endometriosis consecutively (consecutive sampling). Each subject taken from the blood and follicular fluid then measured the levels of homocystein levels with immuoassay method. The mean of each group was statistically tested with an independent t test. Result: The mean levels of homocysteine in the blood is higher in the endometriosis group than without endometriosis group and it was statistically significance (8.34 Â± 2.68 vs 6.71 Â± 1.56, p=0.007; 95% CI: 0.02417-0.14657). Similarly, the levels of homocysteine in follicular fluid , the endometriosis group is higher and statistically significance (6.19 Â± 1.67 vs 3.46 Â± 1.03; p= 0.000; 95% CI: 0.19310-0.32353). All oocytes are in good quality in both groups, maturation grade 3. There is a correlation between the levels of homocysteine in the blood and follicular fluid in the endometriosis group and assessed with Pearson test, and it found significant (p = 0.002) and the correlation value 0.553 (moderate correlation strength) and direction of a positive correlation. Conclusion: The mean levels of homocysteine in the blood and follicular fluid in infertile women with endometriosis is higher than without endometriosis and were statistically significantly different. These homocysteine levels does not affect the quality of oocytes. There is a positive correlation between the levels of homocysteine in the blood and follicular fluid in endometriosis group. [Indones J Obstet Gynecol 2013; 37-2: 92-8] Keywords: endometriosis, homocysteine, infertility, oocyte quality

PPARy Expression in Eutopic and Ectopic Endometrium of Reproductive Age Women with Endometriosis Dilmy, Adya F; Natadisastra, Muharam; Sumapradja, Kanadi
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 4, October 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To evaluate the expression of PPARy receptor and to compare its expression in eutopic and ectopic endometrium in women with endometriosis. Method: This is a cross sectional study. Ten female subjects with endometriosis that underwent laparoscopy or laparotomy who fulfilled the inclusion criteria were recruited by consecutive sampling. Two samples were taken, eutopic endometrium and ectopic endometrium from endometriosis cyst wall during surgery of each subject. PPARy expression was examined by two-step RT-qPCR. Our data was statistically examined using the paired t-test and Pearson’s correlation test. Result: PPARy was found to be expressed in eutopic and ectopic endometrium of women with endometriosis using the RT-qPCR method. The expression of PPARy was not statistically different in eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative fold; p=0.26). By Pearson’s correlation there was a weak positive correlation between PPARy expression of eutopic and ectopic endometrium (r=0.16). Conclusion: PPARy was detected by two-step RT-qPCR in eutopic and ectopic endometrium of women with endometriosis. Semiquantification of PPARy expression showed that there was no significant difference between PPARy expression in eutopic and ectopic endometrium of women with endometriosis. There was a weak positive correlation between PPARy expression in eutopic and ectopic endometrium of women with endometriosis. [Indones J Obstet Gynecol 2015; 3-4: 200-205] Keywords: endometriosis, PPARy, two-step RT-qPCR

Pentoxifylline as a Therapy for Thin Endometrial Lining in Infertility Natadisastra, Muharam; Kurniawan, Riyan H; Malik, Devi M
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 3, July 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To evaluate the effect of pentoxifylline as a single regimen therapy for thin endometrial lining in infertility. Method: A cross-sectional retrospective research was held in Yasmin Clinic - Kencana, Dr. Cipto Mangunkusumo Hospital from 2010 until 2011. Our respondents were women with infertility problem. Result: There was significant improvement of endometrial lining in respondents who received pentoxifylline in the first month (p

Methylation Profile of HOXA 11 Gene in Eutopic Endometrium on Endometriosis Patient with Infertility Natadisastra, Muharam; Yuwono, Valencia; Febri, Ririn; Asmarinah, Asmarinah
Indonesian Journal of Obstetrics and Gynecology Volume 6. No. 2 April 2018
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To investigate the HOXA11 gene profile on endometriosispatients with infertility in Indonesia.Methods: This cross sectional study was conducted in Dr. CiptoMangunkusumo Hospital from July 2015- June 2016. The subjectswere endometriosis patients with infertility who have beenconfirmed histopathological. The control group was taken fromnon-endometriosis and fertile patients. Eutopic endometriumsamples were taken and examined for the methylation of HOXA 11gene.Results: Both groups consist of six patients. The difference ofmethylation of HOXA 11 gene between those two groups isstatistically significant (p=0.03). There was hyper methylation inendometriosis group.Conclusion: There is a hyper methylation of HOXA 11 gene ineutopic endometrium of endometriosis patients with infertility.Thus, possibly can explain the poor endometrial receptivityin endometriosis patient and give a broad research area inepigenetic therapy of endometriosis.[Indones J Obstet Gynecol 2018; 6-2: 110-113]Keywords: endometriosis, epigenetic, HOXA 11, infertility,methylation

The Outcome on Conservative Surgical Treatment of Adenomyosis Wiweko, Budi; Legiantuko, Ario; Kemal, Achmad; Pratama, Gita; Situmorang, Herbert; Sumapraja, Kanadi; Natadisastra, Muharam; Hestiantoro, Andon
Indonesian Journal of Obstetrics and Gynecology Volume 4, No. 4, October 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To understand the outcome on conservative surgical treatment of adenomyosis. Methods: A retrospective cohort study followed for 2 years from 2010 to 2012 of women with adenomyosis were diagnosed by transvaginal sonography and confirmed histologically. Subjects divided into women who were treated by adenomyosis resection (with/without Osada’s technique) and who were underwent hysterectomy. Results: After the surgery, as many as 40 patients (81.63%) did not feel any pain (VAS 0), and 9 patients (18.37%) still felt pain. For the fertility outcome, we had 8 patients (20.51%) getting pregnant naturally without any fertility intervention. Two patients (5.13%) had successfully conceived by IVF. According to the type of surgery, from 8 natural pregnancy, 7 patients (87.50%) was underwent conventional resection of adenomyosis and 1 patients (12.50%) underwent Osada’s procedures. Two patients who were conceived by IVF, both of them were underwent Osada’s resection. Conclusion: Adenomyosis resection both conservative or Osada’s procedures actually has a better outcome for relieving pain; therefore, some patients can still have a child. Keywords: adenomyosis resection, conventional resection, infertility, Osada’s procedure

Homocysteine Level in the Blood and Follicular Fluid is Higher in Infertile Women with Endometriosis Natadisastra, Muharam
Indonesian Journal of Obstetrics and Gynecology Volume. 37, No. 2, April 2013
Publisher : Indonesian Socety of Obstetrics and Gynecology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.231 KB)

Objective: To compare and determine the differences in the level of homocysteine in the blood and follicular fluid in infertile women with and without endometriosis, then analyze the effect of homocysteine levels to oocyte quality. Methods: This study was cross-sectional study. Fifty-nine subjects following the in-vitro fertilization program are included in the admission criteria were divided into two equal groups, ie groups of endometriosis and without endometriosis consecutively (consecutive sampling). Each subject taken from the blood and follicular fluid then measured the levels of homocystein levels with immuoassay method. The mean of each group was statistically tested with an independent t test. Result: The mean levels of homocysteine in the blood is higher in the endometriosis group than without endometriosis group and it was statistically significance (8.34 ± 2.68 vs 6.71 ± 1.56, p=0.007; 95% CI: 0.02417-0.14657). Similarly, the levels of homocysteine in follicular fluid , the endometriosis group is higher and statistically significance (6.19 ± 1.67 vs 3.46 ± 1.03; p= 0.000; 95% CI: 0.19310-0.32353). All oocytes are in good quality in both groups, maturation grade 3. There is a correlation between the levels of homocysteine in the blood and follicular fluid in the endometriosis group and assessed with Pearson test, and it found significant (p = 0.002) and the correlation value 0.553 (moderate correlation strength) and direction of a positive correlation. Conclusion: The mean levels of homocysteine in the blood and follicular fluid in infertile women with endometriosis is higher than without endometriosis and were statistically significantly different. These homocysteine levels does not affect the quality of oocytes. There is a positive correlation between the levels of homocysteine in the blood and follicular fluid in endometriosis group. [Indones J Obstet Gynecol 2013; 37-2: 92-8] Keywords: endometriosis, homocysteine, infertility, oocyte quality

Pentoxifylline as a Therapy for Thin Endometrial Lining in Infertility Natadisastra, Muharam; Kurniawan, Riyan H; Malik, Devi M
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 3, July 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To evaluate the effect of pentoxifylline as a single regimen therapy for thin endometrial lining in infertility. Method: A cross-sectional retrospective research was held in Yasmin Clinic - Kencana, Dr. Cipto Mangunkusumo Hospital from 2010 until 2011. Our respondents were women with infertility problem. Result: There was significant improvement of endometrial lining in respondents who received pentoxifylline in the first month (p

PPARy Expression in Eutopic and Ectopic Endometrium of Reproductive Age Women with Endometriosis Dilmy, Adya F; Natadisastra, Muharam; Sumapradja, Kanadi
Indonesian Journal of Obstetrics and Gynecology Volume. 3, No. 4, October 2015
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To evaluate the expression of PPARy receptor and to compare its expression in eutopic and ectopic endometrium in women with endometriosis. Method: This is a cross sectional study. Ten female subjects with endometriosis that underwent laparoscopy or laparotomy who fulfilled the inclusion criteria were recruited by consecutive sampling. Two samples were taken, eutopic endometrium and ectopic endometrium from endometriosis cyst wall during surgery of each subject. PPARy expression was examined by two-step RT-qPCR. Our data was statistically examined using the paired t-test and Pearson’s correlation test. Result: PPARy was found to be expressed in eutopic and ectopic endometrium of women with endometriosis using the RT-qPCR method. The expression of PPARy was not statistically different in eutopic and ectopic endometrium (1.16 relative fold vs 1.25 relative fold; p=0.26). By Pearson’s correlation there was a weak positive correlation between PPARy expression of eutopic and ectopic endometrium (r=0.16). Conclusion: PPARy was detected by two-step RT-qPCR in eutopic and ectopic endometrium of women with endometriosis. Semiquantification of PPARy expression showed that there was no significant difference between PPARy expression in eutopic and ectopic endometrium of women with endometriosis. There was a weak positive correlation between PPARy expression in eutopic and ectopic endometrium of women with endometriosis. [Indones J Obstet Gynecol 2015; 3-4: 200-205] Keywords: endometriosis, PPARy, two-step RT-qPCR

The Outcome on Conservative Surgical Treatment of Adenomyosis Wiweko, Budi; Legiantuko, Ario; Kemal, Achmad; Pratama, Gita; Situmorang, Herbert; Sumapraja, Kanadi; Natadisastra, Muharam; Hestiantoro, Andon
Indonesian Journal of Obstetrics and Gynecology Volume 4, No. 4, October 2016
Publisher : Indonesian Socety of Obstetrics and Gynecology

Objective: To understand the outcome on conservative surgical treatment of adenomyosis. Methods: A retrospective cohort study followed for 2 years from 2010 to 2012 of women with adenomyosis were diagnosed by transvaginal sonography and confirmed histologically. Subjects divided into women who were treated by adenomyosis resection (with/without Osada’s technique) and who were underwent hysterectomy. Results: After the surgery, as many as 40 patients (81.63%) did not feel any pain (VAS 0), and 9 patients (18.37%) still felt pain. For the fertility outcome, we had 8 patients (20.51%) getting pregnant naturally without any fertility intervention. Two patients (5.13%) had successfully conceived by IVF. According to the type of surgery, from 8 natural pregnancy, 7 patients (87.50%) was underwent conventional resection of adenomyosis and 1 patients (12.50%) underwent Osada’s procedures. Two patients who were conceived by IVF, both of them were underwent Osada’s resection. Conclusion: Adenomyosis resection both conservative or Osada’s procedures actually has a better outcome for relieving pain; therefore, some patients can still have a child. Keywords: adenomyosis resection, conventional resection, infertility, Osada’s procedure

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