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All Journal Folia Medica Indonesiana Biomolecular and Health Science Journal
Etik Ainun Rohmah
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Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health Other

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ISOLATION OF Streptomyces sp. FROM LAPINDO MUD SOIL, SIDOARJO, EAST JAVA PROVINCE, INDONESIA AS A LARVICIDE CANDIDATE AGAINST Aedes aegypti Subagyo Yotopranoto; Rochmah Kurnijasanti; Etik Ainun Rohmah
Folia Medica Indonesiana Vol. 53 No. 2 (2017): JUNE 2017
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (253.549 KB) | DOI: 10.20473/fmi.v53i2.6355

Abstract

Streptomyces sp. is a bacterium that can live in many kind of habitations e.i. marine, sea sponge, coastal area, soil, desert soil, river bank etc. Many antibiotics and secondary metabolites are produced by this bacterium. Several secondary metabolites of the bacterium can be used as an insecticide against insects including mosquito larva.The purpose of this study was to search and isolate until species of Streptomyces sp. from Sidoarjo Lapindo mud soil in East Java province that can be used as a larvicide against Aedes aegypti larva, the dengue haemorrhagic fever vector.The method of study was to collect several mud soil samples from Sidoarjo Lapindo. The collected samples were cultured in ISP-4 media for producing mix-cultures. Then, to isolate the suspected colony of Streptomyces sp. by culturing again in several replications on ISP-4 media in petri-dish. The pure isolates were cultured in ISP-4 slant media. There were could be obtained eight pure isolates of Streptomyces sp.The characterization of 16S rRNA of Streptomyces sp. was done in order to determine the species. DNA isolation was done and followed by DNA sequencing, then compared to Gene Bank with BLAST program. The results showed that the sequence nucleotide bases of Streptomyces Sp-D6 had high similarity to Streptomyces sp. 171524, beside the sequence nucleotide bases of Streptomyces Sp-D7 and Sp-D9 had high similarity to Strepto-myces sp. ACT-01578 and ACT-175695. These three species can be used as larvicide candidate against Ae. aegypti.
Breeding Preference and Bionomics of Anopheles spp. at the Malarial Endemic Area, Runut Village, East Nusa Tenggara Province, Indonesia Gery Morsales Munthe; David Nugraha; Gabriel Pedro Mudjianto; Etik Ainun Rohmah; Arnoldina Dolfina Dua Weni; Zukhaila Salma; Lynda Rossyanti; Fitriah; Suhintam Pusarawati; Budi Utomo; ukmawati Basuki; Haruki Uemura
Biomolecular and Health Science Journal Vol. 5 No. 1 (2022): Biomolecular and Health Science Journal
Publisher : Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bhsj.v5i1.35278

Abstract

Introduction: Anopheles mosquito is transmitting malaria, one of the health problems in Indonesia. Understanding Anopheles mosquito behaviour and its breeding preference is one of the crucial keys to prevent malaria transmission. This study aimed to identify the breeding place distribution and bionomics of Anopheles spp. in Runut village, Sikka district, East Nusa Tenggara. Methods: A descriptive observational study was conducted in Runut village, Waigete sub- district, in April 2018. Anopheles spp. larvae were collected in 7 suspected breeding places consisting of 2 rice fields, 3 fish ponds, and 2 puddles. Mosquitos behaviour was observed using bed-net traps located inside and outside the house from 7.15 PM to 1.15 AM after obtaining informed consent. Mosquito collection using bed-net trap were performed for 40 minutes then followed by resting mosquito collection for 10 minutes. Results: Anopheles spp. larvae were found in most of the suspected aquatic habitats, presenting different densities and together with larvae of the other mosquito species. Relatively high number of Anopheles spp. larvae was obtained from a puddle. Only one female mosquito of Anopheles spp. resting on the wall inside house was found around 00.55 – 01.05 AM and resulted in low mosquito density determination. Conclusion: Anopheles spp. larvae were harbouring in most of the aquatic habitats and one puddle contained moderately abundant larvae of Anopheles in Runut village, Sikka district, East Nusa Tenggara, Indonesia. Even only one Anopheles spp. mosquito was detected inside the house, residents in Runut village should regular use insecticide-treated bed nets and continuous observation of mosquito breeding places especially puddles to prevent malaria and other mosquito-borne diseases.
Co-Authors Arnoldina Dolfina Dua Weni BUDI UTOMO David Nugraha Fitriah Gabriel Pedro Mudjianto Gery Morsales Munthe Haruki Uemura Rochmah Kurnijasanti ROSSYANTI, LYNDA Subagyo Yotopranoto Suhintam Pusarawati ukmawati Basuki Zukhaila Salma