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All Journal Indonesian Journal of Tropical and Infectious Disease journal of Basic Medical Veterinary AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment)
Dadik Raharjo
Department Of Veterinary Public Health, Faculty Of Veterinary Medicine, Universitas Airlangga
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Electrical & Electronics Engineering Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Antimicrobial Activities of Laurus nobilis Leaves Ethanol Extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. khawla Abdullah Sakran; Dadik Raharjo; Ni Made Mertaniasih
Indonesian Journal of Tropical and Infectious Disease Vol. 9 No. 2 (2021)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v9i2.22873

Abstract

Laurus nobilis is one of the most well-known, most frequently used plants is from Lauraceae family which contains up 2.500 species that grow in the subtropics and tropics of the Mediterranean region and Indonesia. This study was supposed to investigate the antimicrobial eff ect of L.nobilis leaves ethanol extract on Staphylococcus aureus, Salmonellae typhi, and Escherichia coli. This preliminary study examined the antimicrobial eff ect of L.nobilis leaves ethanol extract. The method used Agar-well diff usion for determination of the zone of inhibition and the minimum bactericidal concentration to investigate the activity of L.nobilis leaves ethanol extract at 100% concentration . The results revealed that extract of L. nobilis leaves had the antibacterial activity against Staphylococcus aureus with a zone of inhibition (16.3 ±1.5 mm), Staphylococcus aureus with (14.5±0.5 mm), and weak antimicrobial activity against Escherichia coli (11.3±1.1mm). Also, through the minimum bactericidal concentration experiment, the L.nobilis leaves ethanol extract had activity on Staphylococcus aureus and Salmonellae typhi, it’s killed the bacteria in all concentration start it from 5×107 to 5×104. But the activity on Escherichia coli just weaken concentration 5×107 and 106. This research has concluded that the L.nobilis leaves ethanol extract exhibited a signifi cant antimicrobial eff ect against Staphylococcus aureus and Salmonellae typhi then Escherichia coli that is considered a kind of multidrug-resistant bacteria.
Total Plate dan Total Staphylococcus aureus pada Daging Di Pasar Tradisional Kecamatan Mulyorejo Surabaya Nonie Olivia Adia Mutiarasari; Nenny Harijani; Fedik Abdul Rantam; Dadik Raharjo; Agnes Theresia Soelih Estoepangestie; Didik Handijatno
Journal of Basic Medical Veterinary Vol. 9 No. 2 (2020): Journal of Basic Medical Veterinary, December 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v9i2.28584

Abstract

This study aimed to evaluate the Total Plate Count and total Staphylococcus aureus count of beef sold in wet markets in Mulyorejo sub-district below the National Standard Indonesia (SNI 7388:2009) about maximum limit of microbial contamination in food or not. Total of twenty four samples of beef purchased from traditional markets of Tempurejo, Krempyeng Yamuri, Pacar Keling, and Menur in Mulyorejo sub-district Surabaya were examined by Total Plate Count using pour plate method. The sample was also cultured in Mannitol Salt Agar. The colony suspected to be S. aureus were taken for identification. The identification of S. aureus consists of isolation in Mannitol Salt Agar, Gram staining, catalase test, and coagulase test. Total plate count result showed that four samples were exceeding the National Standard of Indonesia SNI 7388:2009 or 1x106 CFU/g and the rest were below the maximum Total Plate Count in SNI. The highest Total Plate Count result was 1,9x106 CFU/g and the lowest was 7,8x104 CFU/g. The result of identification showed that 100% samples examined were contaminated by S. aureus with the highest result was 2,9x104 CFU/g and the lowest result was 4,3x103 CFU/g or exceeding the SNI 7388:2009.
Detection of eae Gene as A Marker Strain EPEC (Enteropathogenic Escherichia coli) in Chicken Meat Sold at Some Traditional Markets in Surabaya Amirul Muslim Amrullah; Fedik Abdul Rantam; Dadik Raharjo; Wiwiek Tyasningsih; Agnes Theresia Soelih Estoepangestie; Martia Rani Tacharina
Journal of Basic Medical Veterinary Vol. 11 No. 1 (2022): Journal of Basic Medical Veterinary, June 2022
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v11i1.31134

Abstract

This study aimed to detect the presence of eae gene in E. coli from chicken meat sold at the traditional market Surabaya. Identification of bacteria was carried out in several stages, inoculated on pre-enrichment media using Buffered Peptone Water (BPW), planting on Levine’s Eosin Methylene Blue Agar (L-EMB) media, suspected colonies on L-EMB were subjected to Triple Sugar Iron Agar (TSIA) and Indol test for the confirmation of E. coli. The confirmed E. coli isolates were tested by Polymerase chain reaction (PCR) to determine the presence of the eae gene. The results showed that E. coli could be identified in 28 out of 100 (28%) chicken meat samples, and eae gene was detected in three E. coli isolates (11%).
Occurrences Salmonella sp. and Escherichia Coli in Bulk and Packaged Chicken Sausages in Surabaya, Indonesia Deana Fyra Adi Nur; Ratna Yulistiani; Dedin F. Rosida; Dadik Raharjo
AJARCDE (Asian Journal of Applied Research for Community Development and Empowerment) Vol. 6 No. 2 (2022)
Publisher : Asia Pacific Network for Sustainable Agriculture, Food and Energy (SAFE-Network)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (425.656 KB) | DOI: 10.29165/ajarcde.v6i2.99

Abstract

Salmonella sp. and Escherichia coli are pathogenic bacteria that cause foodborne diseases that often contaminate food and are harmful to human health. Sales of unpackaged (bulk) sausages accompanied by poor hygiene and environmental sanitation conditions have a high potential for Salmonella and Escherichia coli contamination. The purpose of this study was to compare the contamination level of bacterial, Salmonella sp. and Escherichia coli in bulk and packaged chicken sausages as well as to determine the relationship between the hygiene and sanitation of traders and the level of bacterial contamination, Salmonella sp. and Escherichia coli contamination in chicken sausages sold at traditional markets in Surabaya, Indonesia. This research is a cross-sectional study and purposive sampling method. The results showed that the level of bacterial contamination in the bulk chicken sausage was higher (5.98 Log CFU/g) than in packaged chicken sausage (4.83 Log CFU/g). Salmonella sp. contamination in the bulk chicken sausage was higher (44.44%) than in packaged chicken sausage (10.00%) and Escherichia coli contamination in the bulk chicken sausage was higher (22.22%) than in packaged chicken sausage (20.00%). There is a significant relationship between the hygiene and sanitation of traders with the contamination level of bacteria, Salmonella sp., and Escherichia coli in bulk and packaged chicken sausages.
Co-Authors Agnes Theresia Soelih Estoepangestie Amirul Muslim Amrullah Deana Fyra Adi Nur Dedin F. Rosida Didik Handijatno Fedik Abdul Rantam khawla Abdullah Sakran Martia Rani Tacharina Nenny Harijani Ni Made Mertaniasih Nonie Olivia Adia Mutiarasari Ratna Yulistiani Tyasningsih, Wiwiek