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EFEK EKSTRAK ETANOL BIJI LABU KUNING (CUCURBITA MOSCHATA DUCHESNE) SEBAGAI ANTELMINTIK PADA CACING GELANG (ASCARIDIA GALLI) Noni Zakiah; Vonna Aulianshah; T. Maulana Hidayatullah; Faridah Hanum
Sel Jurnal Penelitian Kesehatan Vol 7 No 1 (2020): SEL Jurnal Penelitian Kesehatan
Publisher : Balai Penelitian dan Pengembangan Kesehatan Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/sel.v7i1.2341

Abstract

Kegunaan labu kuning di Indonesia masih sebatas daging buah yang dapat diolah menjadi panganan seperti kue basah, kolak dan sayur berkuah. Secara empiris, biji labu kuning telah digunakan untuk mengatasi cacingan. Penelitian ini dilakukan untuk mengetahui mortalitas cacing gelang (Ascaridia galli) dalam ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne). Penelitian ini menggunakan 25 ekor Ascaridia galli yang dibagi menjadi 5 kelompok, kelompok I kontrol negatif menggunakan larutan NaCl fisiologis, kelompok II kontrol positif menggunakan larutan pirantel pamoat 0,5 %, kelompok III, IV dan V berturut-turut menggunakan 25 mg/ml, 50 mg/ml dan 100 mg/ml ekstrak etanol biji labu kuning. Parameter penelitian ini ditentukan dengan melihat persentase nilai skor pasca inkubasi 12 jam, 24 jam, dan 36 jam. Skor 3 diberikan apabila seluruh tubuh Ascaridia galli bergerak, skor 2 diberikan jika hanya sebagian tubuh Ascaridia galli bergerak, skor 1 jika Ascaridia galli diam tetapi masih hidup, dan skor 0 apabila Ascaridia galli mati. Hasil uji in vitro dengan perlakuan 25 mg/ml ekstrak etanol biji labu kuning menyebabkan kematian 3 ekor Ascaridia galli atau 60% pasca inkubasi 36 jam, sedangkan ekstrak etanol biji labu kuning dengan perlakuan 50 mg/ml, 100 mg/ml dan kelompok kontrol positif mengakibatkan kematian 4 ekor Ascaridia galli atau 80% pasca inkubasi 36 jam. Dari hasil penelitian disimpulkan bahwa ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne) dosis 25 mg/ml, 50 mg/ml, dan 100 mg/ml secara in vitro dalam waktu 36 jam mampu mengakibatkan mortalitas Ascaridia galli. The use of yellow pumpkin in Indonesia is still limited to fruit meat that can be processed into snacks such as soggy cakes, porridge and vegetable soup. This research was conducted to determine the mortality of Ascaridia galli in ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne). This study used 25 Ascaridia galli which were divided into 5 groups, group I was negative control using physiological NaCl solution, group II was positive control using 0.5% pirantel pamoate solution, group III, IV and V respectively used 25 mg / ml, 50 mg/ml and 100 mg/ml ethanol extract of yellow pumpkin seeds. The parameters of this study were determined by looking at the percentage of post-incubation scores 12 hours, 24 hours, and 36 hours. A score of 3 is given if the whole body of Ascaridia galli moves, a score of 2 is given if only part of the body of Ascaridia galli moves, a score of 1 if Ascaridia galli is still but still alive, and a score of 0 if Ascaridia galli dies. In vitro test results with 25 mg/ml ethanol extract of pumpkin seeds caused 3 deaths of Ascaridia galli or 60% after incubation for 36 hours, while ethanol extract of yellow pumpkin seeds treated with 50 mg / ml, 100 mg/ml and positive control group resulting in the death of 4 Ascaridia galli or 80% after 36 hours incubation. From the results of the study concluded that the ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne) doses of 25 mg / ml, 50 mg / ml, and 100 mg / ml in vitro within 36 hours can lead to Ascaridia galli mortality.
POTENSI ANTIMIKROBA EKSTRAK DAUN JAMBLANG (Syzygium cumini L.) DARI KAWASAN GEOTHERMAL IE SEUM ACEH BESAR Munira Munira; Noni Zakiah; Rini Handayani; Muhammad Nasir
Jurnal Insan Farmasi Indonesia Vol 5 No 1 (2022): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v5i1.915

Abstract

Jamblang leaf (Syzygium cumini L.) contains several antimicrobial compounds. The content of chemical compounds in a plant is influenced by environmental factors. The Geothermal area of Ie Seum Aceh Besar has a different temperature and pH of the soil. This research was conducted to determine the antimicrobial activity of jamblang leaf ethanol extract which grew inside and outside the geothermal area. This research is experimental laboratories using a complete randomized design (RAL). The study consisted of 3 treatments, namely distilled water, jamblang leaf extract which grew in the Geothermal area of Ie Seum (EDJDKG) and jamblang leaf extract that grew outside the Geothermal area of Ie Seum (EDJLKG) and each 5 replications. The results showed that jamblang leaf ethanol extract that grew inside and outside the geothermal area of Ie Seum was very influential (p = 0,000) in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Candida albican. Duncan's further test results showed that the largest average inhibitory diameter was produced by EDJDKG (19.20 mm) and was not significantly different from EDJLKG (18.00 mm) in inhibiting the growth of S. aureus. As for E. coli EDJDKG of 20.70 mm and significantly different from EDJLKG (18.70 mm).
POTENSI ANTIMIKROBA EKSTRAK DAUN JAMBLANG (Syzygium cumini L.) DARI KAWASAN GEOTHERMAL IE SEUM ACEH BESAR Munira Munira; Noni Zakiah; Rini Handayani; Muhammad Nasir
Jurnal Insan Farmasi Indonesia Vol 5 No 1 (2022): Jurnal Insan Farmasi Indonesia
Publisher : Sekolah Tinggi Ilmu Kesehatan ISFI Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36387/jifi.v5i1.915

Abstract

Jamblang leaf (Syzygium cumini L.) contains several antimicrobial compounds. The content of chemical compounds in a plant is influenced by environmental factors. The Geothermal area of Ie Seum Aceh Besar has a different temperature and pH of the soil. This research was conducted to determine the antimicrobial activity of jamblang leaf ethanol extract which grew inside and outside the geothermal area. This research is experimental laboratories using a complete randomized design (RAL). The study consisted of 3 treatments, namely distilled water, jamblang leaf extract which grew in the Geothermal area of Ie Seum (EDJDKG) and jamblang leaf extract that grew outside the Geothermal area of Ie Seum (EDJLKG) and each 5 replications. The results showed that jamblang leaf ethanol extract that grew inside and outside the geothermal area of Ie Seum was very influential (p = 0,000) in inhibiting the growth of Staphylococcus aureus, Escherichia coli and Candida albican. Duncan's further test results showed that the largest average inhibitory diameter was produced by EDJDKG (19.20 mm) and was not significantly different from EDJLKG (18.00 mm) in inhibiting the growth of S. aureus. As for E. coli EDJDKG of 20.70 mm and significantly different from EDJLKG (18.70 mm).