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EFEK EKSTRAK ETANOL BIJI LABU KUNING (CUCURBITA MOSCHATA DUCHESNE) SEBAGAI ANTELMINTIK PADA CACING GELANG (ASCARIDIA GALLI) Noni Zakiah; Vonna Aulianshah; T. Maulana Hidayatullah; Faridah Hanum
Sel Jurnal Penelitian Kesehatan Vol 7 No 1 (2020): SEL Jurnal Penelitian Kesehatan
Publisher : Balai Penelitian dan Pengembangan Kesehatan Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22435/sel.v7i1.2341

Abstract

Kegunaan labu kuning di Indonesia masih sebatas daging buah yang dapat diolah menjadi panganan seperti kue basah, kolak dan sayur berkuah. Secara empiris, biji labu kuning telah digunakan untuk mengatasi cacingan. Penelitian ini dilakukan untuk mengetahui mortalitas cacing gelang (Ascaridia galli) dalam ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne). Penelitian ini menggunakan 25 ekor Ascaridia galli yang dibagi menjadi 5 kelompok, kelompok I kontrol negatif menggunakan larutan NaCl fisiologis, kelompok II kontrol positif menggunakan larutan pirantel pamoat 0,5 %, kelompok III, IV dan V berturut-turut menggunakan 25 mg/ml, 50 mg/ml dan 100 mg/ml ekstrak etanol biji labu kuning. Parameter penelitian ini ditentukan dengan melihat persentase nilai skor pasca inkubasi 12 jam, 24 jam, dan 36 jam. Skor 3 diberikan apabila seluruh tubuh Ascaridia galli bergerak, skor 2 diberikan jika hanya sebagian tubuh Ascaridia galli bergerak, skor 1 jika Ascaridia galli diam tetapi masih hidup, dan skor 0 apabila Ascaridia galli mati. Hasil uji in vitro dengan perlakuan 25 mg/ml ekstrak etanol biji labu kuning menyebabkan kematian 3 ekor Ascaridia galli atau 60% pasca inkubasi 36 jam, sedangkan ekstrak etanol biji labu kuning dengan perlakuan 50 mg/ml, 100 mg/ml dan kelompok kontrol positif mengakibatkan kematian 4 ekor Ascaridia galli atau 80% pasca inkubasi 36 jam. Dari hasil penelitian disimpulkan bahwa ekstrak etanol biji labu kuning (Cucurbita moschata Duchesne) dosis 25 mg/ml, 50 mg/ml, dan 100 mg/ml secara in vitro dalam waktu 36 jam mampu mengakibatkan mortalitas Ascaridia galli. The use of yellow pumpkin in Indonesia is still limited to fruit meat that can be processed into snacks such as soggy cakes, porridge and vegetable soup. This research was conducted to determine the mortality of Ascaridia galli in ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne). This study used 25 Ascaridia galli which were divided into 5 groups, group I was negative control using physiological NaCl solution, group II was positive control using 0.5% pirantel pamoate solution, group III, IV and V respectively used 25 mg / ml, 50 mg/ml and 100 mg/ml ethanol extract of yellow pumpkin seeds. The parameters of this study were determined by looking at the percentage of post-incubation scores 12 hours, 24 hours, and 36 hours. A score of 3 is given if the whole body of Ascaridia galli moves, a score of 2 is given if only part of the body of Ascaridia galli moves, a score of 1 if Ascaridia galli is still but still alive, and a score of 0 if Ascaridia galli dies. In vitro test results with 25 mg/ml ethanol extract of pumpkin seeds caused 3 deaths of Ascaridia galli or 60% after incubation for 36 hours, while ethanol extract of yellow pumpkin seeds treated with 50 mg / ml, 100 mg/ml and positive control group resulting in the death of 4 Ascaridia galli or 80% after 36 hours incubation. From the results of the study concluded that the ethanol extract of yellow pumpkin seeds (Cucurbita moschata Duchesne) doses of 25 mg / ml, 50 mg / ml, and 100 mg / ml in vitro within 36 hours can lead to Ascaridia galli mortality.
Antibakteri PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KULIT MELINJO HIJAU DAN MERAH (Gnetum gnemon L) TERHADAP Staphylococcus aureus: PERBANDINGAN AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL KULIT MELINJO HIJAU DAN MERAH (Gnetum gnemon L) TERHADAP Staphylococcus aureus diva nadia humaira; Noni Zakiah; Vonna Aulianshah
JIFS: JURNAL ILMIAH FARMASI SIMPLISIA Vol. 3 No. 1 (2023): Juni 2023
Publisher : Jurusan Farmasi, Politeknik Kesehatan Kementerian Kesehatan Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Along with the increase in bacterial resistance in the world of health, it is necessary to find new drugs. Melinjo skin has components of bioactive compounds that are thought to have antibacterial potential. this research aims to determine the antibacterial in inhibition ability of the ethanol extract of green and red melinjo peels (Gnetum gnemon L) againts Staphylococcus aureus. This research was experimental, the antibacterial test used the disc diffusion method with a Completely Randomized Design (CRD) which was divided into 3 treatments namely aquades (P0) green melinjo peel extract (P1) red melinjo peel extract (P2) with 6 repetitions each. Phytochemical test results showed that the ethanol extract of green and red melinjo peels contained alkaloids, steroids, terpenoids, flavonoids, phenolics and tannins. The result of the ANOVA test showed that the ethanol extract of green and red melinjo peels was very influential in inhibiting the growth of Staphylococcus aureus (P=0,000). Further Duncan test results showed that the largest average diameter of the inhibiyion zone was the green melinjo peel extract (15,9 mm) and was significantly different from the red melinjo peel extract (10 mm). Green and red melinjo peel extract can inhibit Staphylococcus aureus`
Aktivitas Antibakteri Buah Laban (Vitex pubescens V) yang Tumbuh di Dalam dan di Luar Kawasan Geothermal Ie Seum Aceh Besar terhadap Propionibacterium acnes dan Staphylococcus epidermidis: Antibacterial Activity Of Laban Fruit (Vitex pubescens V) Which Grows In And Outside Geothermal Areas Ie Seum Aceh Besar Against Propionibacterium acnes AND Staphylococcus epidermidis Rasidah; Jeni Windiyasari; Vonna Aulianshah; Munira; Noni Zakiah; Rini Handayani
Indonesian Journal of Pharmacy and Natural Product Vol. 7 No. 01 (2024): Indonesian Journal of Pharmacy and Natural Product
Publisher : Universitas Ngudi Waluyo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35473/ijpnp.v7i01.2619

Abstract

Acne can be caused by clogged skin pores causing red spots, inflammation and infection. Infections of the skin can be caused by the bacteria Propionibacterium acnes and Staphylococcus epidermidis. One plant that has antibacterial potential is laban. Laban plants usually live freely scattered in terrestrial and hilly areas, one of which is in the Ie Seum area in Aceh Besar Regency which is a geothermal area in Aceh. The Ie Seum area has a higher soil pH and temperature compared to other areas (non-geothermal). This study was conducted to determine the difference in antibacterial activity between laban fruit extract (Vitex pubescens V) growing inside and outside the geothermal area of Ie Seum Aceh Besar in inhibiting the growth of Propionibacterium acnes and Staphylococcus epidermidis using disc diffusion method. Microbiological test results obtained the diameter of the inhibitory zone against Propionibacterium acnes and Staphylococcus epidermidis  respectively geothermal laban fruit extract (14.33±1.21 mm and 12.66±1.69 mm) and laban fruit extract outside geothermal (14.83±1.69 mm and 11.41±2.28 mm) and chloramphenicol (19.66±1.88 mm and 22.00±2.50 mm). Anova test results are known to be very influential laban fruit extract (P = 0.000) on the growth of Propionibacterium acnes and Staphylococcus epidermidis. Duncan's further test results found that laban fruit extracts inside and outside the geothermal region had no real difference in inhibiting the growth of Propionibacterium acnes and Staphylococcus epidermidis. The difference in growing place did not provide a difference in antibacterial activity from laban fruit extract (P<0.005).   ABSTRAK Jerawat dapat disebabkan karena tersumbatnya pori-pori kulit sehingga menimbulkan bintik merah, peradangan dan infeksi. Infeksi pada kulit dapat disebabkan oleh bakteri Propionibacterium acnes dan Staphylococcus epidermidis. Salah satu tanaman yang memiliki potensi sebagai antibakteri adalah laban. Tanaman laban biasanya hidup tersebar secara bebas di daerah teresterial dan perbukitan, salah satunya pada kawasan Ie Seum di Kabupaten Aceh Besar yang merupakan daerah geothermal di Aceh. Kawasan Ie Seum memiliki pH tanah dan suhu yang lebih tinggi dibandingkan dengan daerah lainnya (non geothermal). Penelitian ini dilakukan untuk mengetahui perbedaan aktivitas antibakteri antara ekstrak buah laban (Vitex pubescens V) yang tumbuh di dalam dan di luar kawasan geothermal Ie Seum Aceh Besar dalam menghambat pertumbuhan Propionibacterium acnes dan Staphylococcus epidermidis menggunakan metode difusi cakram. Hasil uji mikrobiologi diperoleh diameter zona hambat terhadap Propionibacterium acnes dan Staphylococcus epidermidis berturut-turut ekstrak buah laban geothermal (14,33±1,21 mm dan 12,66±1,69 mm); ekstrak buah laban di luar geothermal (14,83±1,69 mm dan 11,41±2,28 mm) serta kloramfenikol(19,66±1,88 mm dan 22,00±2,50 mm). Hasil uji Anova diketahui ekstrak buah laban sangat berpengaruh (P=0,000) terhadap pertumbuhan Propionibacterium acnes dan Staphylococcus epidermidis. Hasil uji lanjut Duncan diperoleh bahwa ekstrak buah laban di dalam dan di luar kawasan geothermal tidak memiliki perbedaan nyata dalam menghambat pertumbuhan Propionibacterium acnes dan Staphylococcus epidermidis. Perbedaan tempat tumbuh tidak memberikan perbedaan aktivitas antibakteri dari ekstrak buah laban (P<0,005).