Rachmawati Ningsih
Jurusan Kimia Fakultas Sains dan Teknologi Universitas Islam Negeri (UIN) Maulana Malik Ibrahim Malang

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Antioxidant Activity of Flavonoid from Rhizome Kaemferia galanga L. Extract Hayati, Elok Kamilah; Ningsih, Rachmawati; Latifah, Latifah
ALCHEMY ALCHEMY (Vol. 4, No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.957 KB) | DOI: 10.18860/al.v4i2.3203

Abstract

Kaemferia galanga L. rhizome is a traditional crop which is widely used for herbal medicine.  This research is started from the extraction of maceration using ethanol 80% and partition with chloroform:water. 80 % ethanol extract and chloroform fraction tested antioxidant activity with DPPH (1,1-diphenyl-2-picrylhydrazyl), phytochemicals with reagents, separation of the active compound by TLC (thin layer chromatography) analytical and preparative TLC, the identification of compounds with sliding reagent using UV-Vis. The test results on the antioxidant activity of 80% ethanol extract of 64,93% (100 ppm) and chloroform fraction of 54,9 % (100 ppm) with indigo IC50 of 13,07 mg/mL and 81,9 mg mL. 80 % ethanol extract and chloroform fraction has potential as a source of natural antioxidants. Phytochemical test showed 80% ethanol extract contains flavonoids, alkaloids and tannins. At the chloroform fraction extract containing flavonoids. The separation of the active compounds with analytical TLC using eluent PE: ethyl acetate (5: 1) resulted in 7 stain (Rf 0,06 to 0,96) and preparative TLC produce 8 spots (Rf from 0,19 to 0,83). Results from UV-Vis spectrum can be presumed that such a class of flavonoid compounds flavanones or dihidroflavonol. It can be seen from the wavelength range 210-285 nm in the bands I and 300-550 nm in band II. The addition of the reagent slide showed the presence of hydroxyl groups in ring A, B and C and O-hydroxy on ring A. Keywords: antioxidant activity, Kaemferia galanga rizhome, sliding reagent, Thin Layer Chromatography, 1,1-diphenyl-2-picrylhydrazyl 
UJI TOKSISITAS DAN IDENTIFIKASI AWAL GOLONGAN SENYAWA AKTIF EKSTRAK ETANOL DAN N-HEKSANA TERIPANG PASIR (Holothuria scabra) KERING PANTAI KENJERAN SURABAYA Inayah, Nurul; Ningsih, Rachmawati; Adi, Tri Kustono
ALCHEMY ALCHEMY (Vol.2 No.1
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (321.171 KB) | DOI: 10.18860/al.v0i0.2292

Abstract

Sea cucumber (H.Scabra)is a species of marine biota providing high economicaland medical values. It has been hundreds years consumed for drug to cure various diseases in China. The aim of this research was to determine the level of the toxicity against brine shrimp larvae A.salina Leach in each dried sea cucumber (H.scabra) extract collected from Kenjeran Coast, Surabaya. The information would be beneficial for anticancer drug discovery .The research was conducted by extracting samples using both ethanol and n-hexane solvents.The concentrated extract obtained then were used for the toxicity test against brine shrimp larvae of A.salina Leach (BSLT). The mortality data of A.salina Leach was analysed using probit analysis to determine the value of LC50 on each extract. The phytochemical assay and thin layer chromatography (TLC) was also apllied for both extracts.The result showed that both ethanol and n-hexane exctracts performed toxicity level against brine shrimp larvae of A.salina Leach with LC50less than 1000 ppm.The n-hexane extract indicated LC50 of 189.093 ppm, higher than the ethanol extract, 286.031 ppm. The presence of steroids were detected in both extract whilst the alkaloids were only detected in the ethanol extract. Further separation of the n-hexane exctrat using TLC in n-hexane;ethyl acetate;amonia (66:33:0.8) yielded 5 spots with the Rf of 0.3;0.44;0.54;0.72 and 0.78, respectively.
Ekstraksi, Hidrolisis dan Partisi Metabolit Sekunder dari Mikroalga Chlorella sp. Fasya, Ahmad Ghanaim; Dinasti, Anike Riska; Shofiyah, Muharromatus; Rahmawati, Laili Maghfiroh; Millati, Nuria; Safitri, Dany Aulia; Handoko, Singgih; Hanapi, Ahmad; Ningsih, Rachmawati
ALCHEMY Vol 5, No 1 (2016): ALCHEMY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.234 KB) | DOI: 10.18860/al.v5i1.3686

Abstract

Mikroalga Chlorella sp. adalah tumbuhan tingkat rendah yang mengandung metabolit sekunder yaitu senyawa steroid. Penelitian ini bertujuan untuk mendapatkan senyawa metabolit sekunder yang terkandung dalam mikroalga Chlorella sp. berdasarkan kelarutannya terhadap pelarut etil asetat dan petroleum eter. Chlorella sp. dikultivasi dalam Media Ekstrak Tauge (MET) 4% dan pemanenannya dilakukan pada hari ke-10. Biomassa hasil panen diukur kadar air dengan teknik termogravimetri dan dimaserasi menggunakan pelarut methanol. Ekstrak kasar hasil maserasi dihidrolisis dengan HCl 2 N dan dipartisi dengan masing-masing pelarut etil asetat dan petroleum eter. Hasil penelitian menunjukkan bahwa rendemen hasil ekstraksi pada pelarut metanol adalah 21,89 %. Rendemen hasil hidrolisis dan partisi adalah 80,95% untuk fraksi etil asetat dan 50,63% untuk fraksi petroleum eter.
UJI TOKSISITAS DAN IDENTIFIKASI SENYAWA EKSTRAK ALGA MERAH (Eucheuma cottonii) TERHADAP LARVA UDANG Artemia salina LEACH Sharo, Noer Mardlatillah; Ningsih, Rachmawati; Hanapi, Ahmad; Nasichuddin, Ahmad
ALCHEMY ALCHEMY (Vol.2, No.3
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (381.412 KB) | DOI: 10.18860/al.v0i0.2892

Abstract

Eucheuma cottonii is a multicellular algae (multicellular) that have bioactive compounds used in medicine, such as anticancer. The purpose of this research is to know the level of toxicity of the crude extract of ethanol and n-hexane red algae (Eucheuma cottonii) against larvae of shrimp A. salina Leach and to know the active compounds contained in crude extracts of ethanol and n-hexane red algae (Eucheuma cottonii).This research was conducted with the maceration extraction continuing by test toxicity against the shrimp larvae of A. salina Leach (BSLT). The result of the toxicity test then proceeds with the test reagents and phytochemicals with thin layer chromatography for positive test of compound phytochemicals. Thin-layer chromatography using eluen n-hexane: ethyl acetate (7: 3) to ethanol extract and n-hexane: ethyl acetate (17: 3) for n-hexane extract. Data on toxicity of A. salina Leach analyzed by probit analysis to find out the value of LC50 on each extract.The results of this research indicate that each of the rough red algae extract (Eucheuma cottonii) by using a solvent of ethanol and n-hexane have toxicity values (LC50) of shrimp larvae of A. salina Leach consecutive 58,0128 ppm and 61,7571 ppm. Active compounds content of Phytochemical test results i.e. triterpenoid (ethanol extract) and steroids (n-hexsane extract). The TLC triterpenoid obtained 7 stains, and the results obtained steroids 8 TLC stains.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KASAR ETANOL, KLOROFORM DAN N-HEKSANA ALGA COKLAT Sargassum vulgare ASAL PANTAI KAPONG PAMEKASAN TERHADAP BAKTERI Staphilococcus aureus dan Eschericia coli Alfiyaturrohmah, Alfiyaturrohmah; Ningsih, Rachmawati; Yusnawan, Eriyanto
ALCHEMY ALCHEMY (Vol.3, No.1
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (420.679 KB) | DOI: 10.18860/al.v0i0.2906

Abstract

Uji aktivitas antibakteri ekstrak kasar etanol, kloroform dan n-heksan alga coklat S. vulgare asal pantai Kapong Pamekasan dilakukan terhadap bakteri S. aureus dan E. coli. Tujuan penelitian untuk mengetahui ekstrak pelarut terbaik yang mempunyai aktivitas antibakteri serta mengidentifikasi golongan senyawa aktif yang terkandung dalam ekstrak yang berpotensi sebagai antibakteri.Ekstraksi senyawa aktif alga coklat dilakukan dengan metode maserasi pelarut etanol, kloroform dan n-heksana. Ekstrak kasar diuji aktivitas antibakteri menggunakan metode difusi cakram. Senyawa aktif yang dikandung diuji fitokimia dan dipisahkan golongan senyawa menggunakan KLT.Hasil penelitian menunjukkan ekstrak kloroform alga coklat memiliki aktivitas antibakteri terhadap bakteri S. aureus pada konsentrasi 1 % dan terhadap E. coli pada konsentrasi 5%. Hasil uji fitokimia terdeteksi golongan senyawa flavonoid dan steroid. Pemisahan menggunakan KLT eluen kloroform-metanol (99:1) menghasilkan 9 spot dengan nilai Rf 0,017−0,46. Eluen kloroform-metanol (9:1) menghasilkan 7 spot dengan nilai Rf 0,17−0,91.
UJI AKTIVITAS ANTIBAKTERI EKSTRAK KASAR ETANOL, KLOROFORM DAN N-HEKSANA ALGA COKLAT Sargassum vulgare ASAL PANTAI KAPONG PAMEKASAN TERHADAP BAKTERI Staphilococcus aureus dan Eschericia coli Alfiyaturrohmah, Alfiyaturrohmah; Ningsih, Rachmawati; Yusnawan, Eriyanto
ALCHEMY ALCHEMY (Vol.2 No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (672.436 KB) | DOI: 10.18860/al.v0i0.2883

Abstract

Uji aktivitas antibakteri ekstrak kasar etanol, kloroform dan n-heksan alga coklat S. vulgare asal pantai Kapong Pamekasan dilakukan terhadap bakteri S. aureus dan E. coli. Tujuan penelitian untuk mengetahui ekstrak pelarut terbaik yang mempunyai aktivitas antibakteri serta mengidentifikasi golongan senyawa aktif yang terkandung dalam ekstrak yang berpotensi sebagai antibakteri.Ekstraksi senyawa aktif alga coklat S. vulgare dilakukan dengan metode maserasi pelarut etanol, kloroform dan n-heksana. Ekstrak kasar diuji aktivitas antibakteri menggunakan metode difusi cakram. Senyawa aktif yang dikandung diuji fitokimia dan dipisahkan golongan senyawa menggunakan KLT.Hasil penelitian menunjukkan ekstrak kloroform S. vulgare memiliki aktivitas antibakteri terhadap bakteri S. aureus pada konsentrasi 1 % dan terhadap E. coli pada konsentrasi 5%. Hasil uji fitokimia terdeteksi golongan senyawa flavonoid dan steroid. Pemisahan menggunakan KLT eluen kloroform-metanol (99:1) menghasilkan 9 spot dengan nilai Rf 0,017−0,46. Eluen kloroform-metanol (9:1) menghasilkan 7 spot dengan nilai Rf 0,17−0,91.
Extraction, Toxicity Assay and Identification of Active Compounds of Red Algae (Eucheuma cottonii) from Sumenep Madura Afif, Sholeh; Fasya, Ahmad Ghanaim; Ningsih, Rachmawati
ALCHEMY ALCHEMY (Vol. 4, No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (287.673 KB) | DOI: 10.18860/al.v4i2.3199

Abstract

Extraction, Toxicity Assay and Identification ofActive Compounds of Red Algae Extract (Eucheuma cottonii) from Tanjung Sumenep Madura was analysed. Extraction of active compound was conducted by maceration method  using methanol as a solvent. The methanol extract was hydrolyzed with HCl 2 N. The result was partitioned using five solvents (1-butanol, ethyl acetate, chloroform, petroleum  ether and n-hexane). Toxicity level was determined by Brine Shrimp Lethality Test (BSLT). The identification of the active compound was analyzed by phytochemical test, qualitatively. The result showed that the 1-butanol is the best solvent to extract active compounds of red algae E. Cottonii in partition process. 1-Butanol fraction gives the highest activity of toxicity with LC5070,32 ppm. LC50 value for ethyl acetate, chloroform, petroleum ether and n-hexane was 143,43; 194,40; 195,28; 303,66; and 634,97 ppm, respectively. Identification of active compounds indicated that 1-butanol fraction contains steroids and alkaloids. Keywords: Eucheuma cottonii, extraction, Brine Shrimp Lethality Test, phythochemical
UJI TOKSISITAS EKSTRAK DAUN BINAHONG (Anredera cordifolia (Ten.) Steenis) TERHADAP LARVA UDANG Artemia salina Leach DENGAN METODE BRINE SHRIMP LETHALITY TEST (BSLT) Jazilah, Nur; Fasya, Ahmad Ghanaim; Ningsih, Rachmawati; Abtokhi, Ahmad
ALCHEMY ALCHEMY (Vol.3, No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (476.637 KB) | DOI: 10.18860/al.v0i1.2909

Abstract

Daun binahong (Anredera cordifolia (Ten.) Steenis) adalah bagian dari tanaman obat potensial yang dapat mengatasi berbagai jenis penyakit termasuk sebagai obat antikanker. Penelitian ini dilakukan dengan tujuan untuk: (1) Mengetahui tingkat toksisitas masing-masing ekstrak daun binahong (Anredera cordifolia (Ten) Steenis) dalam tiap pelarut n-heksana, etil asetat dan etanol terhadap tingkat mortalitas larva udang Artemia salina Leach. (2) Mengetahui golongan senyawa yang terdapat dalam ekstrak daun binahong (Anredera cordifolia (Ten) Steenis) dengan toksisitas yang terbaik.Penelitian dilakukan dengan mengekstraksi sampel dengan pelarut n-heksana yang dilanjutkan dengan pelarut etil asetat dan etanol. Ekstrak pekat yang diperoleh digunakan untuk uji toksisitas terhadap larva udang BSLT dan uji fitokimia dengan reagen. Data kematian Artemia salina dianalisis dengan analisis probit untuk mengetahui nilai LC50.Hasil dari penelitian menunjukkan pada masing-masing ekstrak daun binahong (Anredera cordifiola (Ten) Steenis) memiliki tingkat toksisitas terhadap larva udang Artemia salina Leach, ditunjukkan dengan nilai LC50 1000 ppm. Tingkat toksisitas ekstrak etanol, ekstrak etil asetat dan ekstrak n-heksana yaitu dengan nilai LC50 sebesar 7,35702 ppm, 106,992 ppm dan 175,800 ppm. Pada ekstrak etanol dilakukan uji fitokimia denga reagen yaitu adanya golongan senyawa alkaloid, flavonoid, tanin, triterpenoid/steroid dan saponin. Golongan senyawa-senyawa tersebut yang menunjukkan adanya potensi bioaktivitas terhadap larva udang Artemia salina Leach. 
SINTESIS SENYAWA ALIL ISOEUGENOL ETER DAN PRODUK REAKSI PENATAAN ULANG CLAISENNYA Ningsih, Rachmawati
ALCHEMY ALCHEMY (Vol.1 No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.262 KB) | DOI: 10.18860/al.v0i0.1673

Abstract

Telah dilakukan penelitian untuk mempelajari reaksi alilasi isoeugenol dan produk penataan ulang Claisennya. Reaksi substitusi gugus alil pada isoeugenol dilakukan dengan pemanasan pada suhu 55oC selama 8 jam. Reaksi penataan ulang Claisen alil isoeugenol eter dilakukan selama 30 menit. Produk reaksi dianalisis dengan spektrofotometer IR, GC dan GC-MS. Pada spektra GC-MS hasil reaksi yang didukung oleh spektra IR, menunjukkan bahwa reaksi alilasi isoeugenol menghasilkan produk campuran alil isoeugenol eter dan 6-alil-isoeugenol dengan kemurnian masing-masing 11,19 % dan 82,13 %. Analisis GC-MS memberikan dua puncak yang memiliki berat molekul sama, yaitu 204 gram/mol. Pemanasan produk hasil alilasi isoeugenol pada suhu 200oC memberikan kemurnian sebesar 60,33 %.
ANTIBACTERIAL ACTIVITY TEST SEA CUCUMBER EXTRACT (Holothuria scabra) SIDAYU COAST GRESIK USING DISK DIFFUSION METHOD Arifin, Haris Nursyah; Ningsih, Rachmawati; Fitrianingsih, Avin Ainur; Hakim, Abdul
ALCHEMY ALCHEMY (Vol.2 No.2
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.952 KB) | DOI: 10.18860/al.v0i0.2882

Abstract

Sea cucumber (Holothuria scabra) is one kind of sea cucumbers that contains of bioactive compound that can inhibit bacteria growth. This research aim to know the effect of methanol and n-hexane extract towards bacterium Staphylococcus aureus, Salmonella typhi and Escherichia coli bacterial growth and to identification estimation active compound group.Sea cucumbers active compound extraction using maceration method with methanol and n-hexane as a solvent. Methanol and n-hexane crude extract conducted antibacterial assay using disk diffusion method towards bacterium Staphylococcus aureus, Salmonella typhi, Escherichia coli and continued with reagent test to knowing active compound group.The result of this research indicated that n-hexane extracts provide the best inhibition zone toward bacterium Escherichia coli at concentration 2000 ppm with among of inhibition zone 1,75 mm, while at bacterium Staphylococcus aureus and Salmonella typhi does not provide inhibition zone. Methanol extract does not provide inhibition zone towards bacterium Staphylococcus aureus, Salmonella typhi and Escherichia coli. The result of reagent test indicated compound that detected in n-hexane extract is saponins