Rena Normasari
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Study of Kidney Repair Mechanism of Cassava Leaf Extract on Gentamicin induced Nephrotoxicity Rena Normasari; Rosita Dewi; Sheilla Rachmania
Journal of Agromedicine and Medical Sciences Vol 3 No 1 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i1.4090

Abstract

Drugs consumption like gentamicin caused damage and decreased of kidney function. Cassava leaf contain lots of micronutrients, such as vitamin C, vitamin A and carotenoids, which also as an antioxidant. The results of the study conducted by Almashuri proved that cassava leaf have high levels of carotenoid compounds. The purpose of this study was to determine the effect of cassava leaf extract on the repairment of renal damage in mice in vivo. This research is true experimental using mice (mus musculus) and were grouped into 5 groups. Two control groups (positive and negative) and 3 treatment groups (cassava leaves 150, 300, and 450 mg / day). Gentamicin is given every day for 14 days and then observed on serum levels of creatinine along with histopathological scores of kidneys’ damage. Statistical analysis showed a significant reduction in serum levels of creatinine (P <0.05) and a significant decrease in renal histopathologic score. This study suggests that cassava leaf extract can repair kidney damage as a result gentamicin-induced nephrotoxicity in mice.Keywords: cassava leaf extract, creatinin, kidney histopathology 
Protective Effect of Cassava Leaf Extract on Gentamicin-Induced Hepatotoxity In Mice Rosita Dewi; Rena Normasari
Journal of Agromedicine and Medical Sciences Vol 5 No 3 (2019)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v5i3.13815

Abstract

Abstract Gentamicin usage can cause the damage of liver structure and function. The basic mechanism inducing liver damage from gentamicin is lipid peroxidation in cell membrane and the suppresion of antioxidant defence system in liver. Antioxidant in cassava leaf such as vitamin C, carotene, flavonoid, dan mineral can protect liver from drug toxicity effect. This research aimed to determine hepatoprotective effect of cassava leaf through microscopic observation of liver histopathology slide of mice induced by gentamicin. The research design was post test only control group design. Mice were divided into five groups, normal group, positive control (gentamicin 80 mg/kg b.w.); P1, P2, and P3 (gentamicin 80 mg/kg b.w. and cassava leaf extract 150 mg/kg b.w., 300 mg/kg b.w., 450 mg/kg b.w. respectively, for 14 days). The average score of liver cell damage was determined by microscopic observation of 200 liver cells undergoing parenchymal degeneration, hidrophic degeneration, and necrosis. One Way Anova analysis showed significant difference among the groups (p<0,05) and Post Hoc Tukey HSD test showed that cassava leaf extract at the dose level of 450 mg/kg b.w. resulted significant liver cell characteristic improvement in liver histophatology slide (p<0,05) compared to positive control group. It could be concluded that cassava leaf extract had protective effect on gentamicin-induced hepatotoxicity in mice. Keywords: cassava leaf extract, hepatotoxicity, gentamicin, liver histopathology
Acute Toxicity of Ethanol Leaves Extracts of Cassava (Manihot esculenta) on Liver Histopathology of Wistar Rats Sheillavi Fauziah Alex Saddamiah; Rena Normasari; Cholis Abrori
Journal of Agromedicine and Medical Sciences Vol 4 No 1 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i1.6741

Abstract

Cassava leaves (Manihot esculenta) contain cyanogenic glylcoside as toxicant. Cyanogenic glycoside can be hydrolized into glucose and cyanohydrin acetone which is then decomposed into cyanide. Cassava leaves are used by the community as a source of food and traditional medicine. However, there is a report of incidents of intoxication and death from cassava consumption in Kenya. This research is intended to know the effect of toxic dose ethanol extract of cassava leaves to liver histopathology of Wistar rats. This study was conducted through two stages, sighting study and main study. Both studies used initial dose level 2000 mg/kg and given once at the first day of each study. Main study consists of two groups, control group given 0,5% Na-CMC and treatment group given ethanol extract of cassava leaves dose 2000 mg/kg. All animals were observed for 14 days. Liver histopathology scores were obtained. Mann Whitney test showed that p= 0,009 (p<0,05) so that can be concluded that ethanol extract of cassava leaves dose 2000 mg/kg can cause histopathological changes in the liver of Wistar rats represented by cloudy swelling degeneration, hydropic degeneration, and necrosis in some cell.
The Protection Effect Of Methanol Extract From Asam Jawa Seed On Testicular Tissue Damage Induced By Aluminium Chloride (AlCL3) Rena Normasari; Muhammad Iqbal Fauzi; Ayu Munawaroh Aziz
Journal of Agromedicine and Medical Sciences Vol 7 No 1 (2021)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v7i1.19645

Abstract

Aluminum is known has neurotoxin effect that can lead to alzheimer's disease, dementia, and parkinson's. Metal ions such as aluminum can distract homeostasis and increase oxidative stress level, formation of reactive oxygen species (ROS) that can overwhelm antioxidant defenses and cause DNA damage, lipid peroxidase, leading to the destruction of testicular cells. Tamarindus indica’s seeds are one of the traditional plant and useful as antioxidants. Tamarindus indica’s seeds are able to protect testicular cells from damage caused by ROS. The purpose of this study to determine the effect of methanol extract from Tamarindus indica’s seeds to prevent aluminum chloride induced testicular damage to be measured from the histopathological features. This study is a quasi experimental design with a posttest design only with non-equivalent groups using thirty male Wistar rats divided into five groups, one control group and four treatment groups. The control group was given 1 mL of aquabides solution orally. Group P1 was given a dose of aluminum solution 300 mg/kgBW orally. Groups P2, P3, and P4 were each given an aluminum solution at dose of 300 mg/kgBW orally and an extract solution at a dose of 25, 50, 100 mg/kgBW orally for 10 weeks. The results of the observations showed that the scores varied between groups. Data analysis using Kruskal-Wallis test obtained p value = 0.006 (p <0.05). Post Hoc test using Mann Whitney obtained varied p values. Post Hoc analysis between K and P1 groups, P1 and P3 groups, P1 and P4 groups showed a significant difference with p value <0.05. Based on the results of this study, methanol extract from tamarind seeds (Tamarindus indica) that was given to male wistar rats had prevented histopathological damage to the testes of rats induced by aluminum chloride (AlCl3). Keywords: aluminium, testicular cell, Tamarindus indica
The Effectiveness of Tamarindus indica Extract in Total Osteoblast Cell of Male Wistar Rat’s Femur Bone Induced by Aluminium Indah Pratiwi; Rena Normasari; Rony Prasetyo
Journal of Agromedicine and Medical Sciences Vol 6 No 2 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i2.17813

Abstract

Aluminum is widely used in life but includes non-essential metals toxic to the human body that most accumulates in the femur. Aluminum is toxic because it increases the number of free radicals resulting in oxidative stress that causes osteoblast apoptosis. Tamarindus indica has the most potent antioxidant effect on the seed. The polyphenol content in Tamarindus indica seed are myricetin, procyanidin B2, and caffeic acid. Antioxidants in Tamarindus indica seed have mechanism of donating electrons from the -OH group in the phenolic ring to stop oxidative chain reactions and prevent the formation of hydroxyl radicals and lipid peroxidation that play a role in cell apoptosis. The purpose of this study to determine the effect of giving Tamarindus indica extract on the total osteoblasts of femur bone in male wistar rats induced by aluminum. This research is a true experimental in vivo with randomized post-test only control group design using 25 male Wistar rats divided into five groups. Aluminum is given orally dose 300mg/kgBB and Tamarindus indica extract dose 25, 50, 100mg/kgBB for 10 weeks. The results of this study indicate the average total of osteoblasts in groups K, P1, P2, P3, P4 are 15.21 ± 1.71; 18.48 ± 3.65; 17.8 ± 7.05; 17.13 ± 1.16; 16.74 ± 5.71. Data analysis used the Kruskal Wallis test. The results of data analysis showed that there was no significant differences in all groups (p = 0.581). Based on the results of this study, Tamarindus indica extract that was given to male wistar rats induced by aluminum for 10 weeks had no effect on the total osteoblasts.
The Difference of Asphyxia Neonatorum Incident between Very Preterm Labor That Is Followed or Not by Premature Rupture of Membranes at RSD dr. Soebandi of Jember Nely Masruroh; Yonas Hadisubroto; Rena Normasari
Journal of Agromedicine and Medical Sciences Vol 4 No 3 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i3.6394

Abstract

Asphyxia neonatorum is an infant's inability to breathe spontaneously and regularly soon after birth. Risk factors of neonatal asphyxia are preterm labor and premature rupture of membranes. In the poor and developing countries there are many occurrences of asphyxia at gestation less than 32 weeks (very preterm). The aim of this study is to describe the incidence of asphyxia neonatorum in very preterm labor followed by premature rupture of membranes, describes the incidence of asphyxia neonatorum in very preterm labor that is not followed by premature rupture of membranes, and the difference of asphyxia neonatorum incident between very preterm labor that is followed or not by premature rupture of membranes. The type of this research was retrospective analytical observational with cross sectional approach and total sampling technique. The population was a single preterm delivery mother with spontaneous presentation of heads born in RSD dr. Soebandi from January 2015 - January 2017. From 465 mothers, 65 mothers meet the inclusion criteria. Data is processed and presented in the form of frequency distribution table then analyzed using Chi-Square test. The results showed that mothers with very preterm followed by premature rupture of membranes did not have asphyxia (22 mothers or 33.8%) and mothers with very preterm that is not followed by premature rupture of membranes did not have asphyxia (18 mothers or 27.2%). Based on Chi-Square statistical test results obtained p-value (0.298)> α (0.05) and it can be concluded that is statistically at 95% confidence level there is no significant difference in the incident of asphyxia neonatorum between very preterm labor that is followed or not by premature rupture of membranes in RSD dr. Soebandi Kabupaten Jember.Keywords: Asphyxia neonatorum, preterm labor, premature rupture of membranes
The Effect of Zumba Exercise to Women's Waist Hip Ratio Laksmi Indreswari; Yunita Dewi Anggraeni; Rena Normasari
Journal of Agromedicine and Medical Sciences Vol 6 No 2 (2020)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v6i2.17449

Abstract

Abdominal obesity is a global public health problem. The high prevalence of abdominal obesity triggers an increase in morbidity and mortality in chronic diseases such as diabetes mellitus, cardiovascular, and cancer. One of the causes of the high prevalence of abdominal obesity is the lack of physical activity. Prevention efforts are needed through the identification of risk factors such as measurement of waist hip ratios. The respondents of this study were divided into two groups: 28 respondents who did zumba exercise for four weeks and 28 control respondents. The statistical test performed was a paired t-test comparison test. The results of this study indicate the value of t-count > t-table value (7.184 > 2.052) and the significance of 0.000 < 0.05. These results indicate that there is a decrease in the waist hip ratio after doing zumba exercise for four weeks.
The Difference in Lemuru Fish Oil Duration of Administration on Connective Tissue Fibroblast Cell Count in Rheumatoid Arthritis Rat Model Asyirah Mujahidah Fillah; Rena Normasari; Dwita Aryadina Rachmawati
Journal of Agromedicine and Medical Sciences Vol 3 No 2 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i2.5062

Abstract

Rheumatoid arthritis is a joint chronic inflammation process leadingto progressive distruction.Cytokinessecreted from chronic inflammation increases fibroblast cell number that synthesize matrix metalloproteinase. Non steroidal anti inflammation drugs administrated to lighten inflammation symptoms had several side effects that increase the urge to discover an alternative antiinflammation with lower side effect like lemuru fish oil. This true experimental study with randomized post test only control group design aimed to investigate the difference in lemuru fish oil duration of administration on connective tissue fibroblast cell count in rheumatoid arthritis rat model. The One Way Anovatestrevealed that there are at least two group means that are statistically significant different from each other with a p value of 0,004. Post Hoc LSD analysis result indicate a significant difference after21 days duration of administrationcompared to 7 days administration with significance value of 0,038.It can be concluded that there is a significant difference in lemuru fish oil administration after21 days duration.
Effect of Leg Length Discrepancy to the Hip Function on Post Hemiarthroplasty Patients in Jember Yosalfa Adhista Kurniawan; Muhammad Hasan; Rena Normasari
Journal of Agromedicine and Medical Sciences Vol 3 No 2 (2017)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v3i2.5068

Abstract

Hip fractures in elderly patient are often treated with hemiarthroplasty. Hemiarthroplasty can cause various complications, one of which is leg legth descrepancy (LLD). The function of the hip joint is reduced due to these complications. Previous studies have shown contradictive results regarding the relationship of LLD to the function of the hip joint in post-arthroplasty patient's. This type of research is observational with retrospective study. The population in this study came from 2 hospitals in Jember was 53 patients and the sample was 30 people. LLD is measured by true leg length and apparent leg length, whereas hip joint function is measured by Oxford Hip Score (OHS). The data were tested by using Spearman correlation test. In conclusion, there was a moderate relationship between LLD true leg length to the function of the hip joint. This study does not support the implications of the LLD to the hip function because of weak relationship between LLD true leg to the hip function and moderate relasionship between LLD apparent leg length to the hip function.
Histopathological Changes in the Wistar Rats Liver Treated with Bangle (Zingiber cassumunar Roxb.) Rhizome Ethanol Extract during Acute Toxicity Test Kesy Sasta Handani; Wiwien Sugih Utami; Bagus Hermansyah; Rena Normasari
Journal of Agromedicine and Medical Sciences Vol 4 No 1 (2018)
Publisher : Faculty of Medicine, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/ams.v4i1.6327

Abstract

Malaria is still an infectious disease that can not be eradicated. The presence of antimalarial drug resistance case encourages the research on herbal plants that are expected to be a complementary therapy drug of malaria, one of them is bangle (Zingiber cassumunar Roxb.). This research aimed to investigate the effect of bangle (Zingiber cassumunar Roxb.) rhizome ethanol extract to the histopathological changes of Wistar rats liver during acute toxicity test. This research type was true experimental laboratories with posttest only control group design using four treatment groups (given dose 42,609 mg/200gBW + Tween 1%, 213,045 mg/200gBW + Tween 1%, 426,09 mg/200gBW + Tween 1%, and 852.18 mg/200gBW + Tween 1%) and one control group (given 1% Tween). Each treatment groups consisted of five male rats and five female rats. The final mean score of hepatocyte damage as the research data was analyzed by One-Way ANOVA test and showed that p=0,084. It indicated that there was no significant difference of liver histopathology results between groups. Keywords: malaria, bangle rhizome, acute toxicity test, liver histopathology