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All Journal Jurnal Pengabdian Kepada Masyarakat Sakai Sambayan Journal of Aquatropica Asia e-Jurnal Rekayasa dan Teknologi Budidaya Perairan Jurnal Pengabdian Fakultas Pertanian Universitas Lampung
Hilma Putri Fidyandini
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Earth & Planetary Sciences Immunology & microbiology Medicine & Pharmacology Other

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Journal : Journal of Aquatropica Asia

 1 
SKRINING BAKTERI TERMOFILIK POTENSIAL AMILOLITIK DARI SUMBER AIR PANAS WAY BELERANG KALIANDA LAMPUNG SELATAN Sefi Desfeni Mawati; Esti Harpen; Hilma Putri Fidyandini
Journal of Aquatropica Asia Vol 6 No 1 (2021): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/aquatropica.v6i1.2458

Abstract

Thermophilic bacteria that produced amylase and protease have been isolated from Way Belerang hot spring, Kalianda, South Lampung. This research aims to screen and identify thermophilic bacteria that have the potential to produce thermostable amylase and protease enzymes.The research procedures included sampling, isolation of enzyme-producing thermophilic bacteria, a series of phenotypic and biochemical tests, and molecular identification by 16s rRNA. This study used 2 treatments, namely incubation temperature 37 and 50 ºC with 3 repetitions. The results showed that the optimum temperature for growth of thermophilic bacterial isolates and thermophilic bacterial isolates producing amylase enzymes was 50ºC. The bacteria isolate that had the best amylolytic enzyme activity was Isolate A.WB.50.1 with a diameter of the inhibitory zone was 15.44 mm. Isolate A.WB.50.1 has been identified by the species Pseudomonas stutzeri.
UJI IN VITRO AKTIVITAS ANTIBAKTERI EKSTRAK CANGKANG BIJI KARET DAN BIJI KARET TERHADAP Aeromonas hydrophila Hilma Putri Fidyandini; Lisa Silviana
Journal of Aquatropica Asia Vol 6 No 1 (2021): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/aquatropica.v6i1.2459

Abstract

Catfish is one of leading fishery commodity in Indonesia that nowadays is using intensive technology. One of the problems in cultivating catfish is the invasion of bacteria Aeromonas hyprophila which can cause Motile Aetomonas Septicemia disease. Information regarding the antibacterial activity of shell and rubber seed extract on Aeromonas hydrophila is not yet known. Therefore it is necessary to do a research about the antibacterial activities of the shell and rubber seed extract against the pathogenic bacteria, Aeromonas hydrophila. This research method was sample preparation, extraction, phytochemical test, preparation of bacterial suspension, antibacterial test with agar diffusion method, minimum inhibitory concentration test. The result shows that the rubber seed shell extract contains antibacterial compounds such as saponin, terpenoids and flavonoids. While the rubber seed extract contains terpenoid and flavonoid compounds. The shell and rubber seed extract at a concentration of 0.5-4 mg/mL showed very weak inhibition.
PENGGUNAAN EKSTRAK DAUN JAMBU BIJI (Psidium guajava Linnaeus) SEBAGAI IMUNOSTIMULAN IKAN MAS (Cyprinus carpio L) YANG DIINFEKSI MOTILE AEROMONAS SEPTICEMIA Rissa Amelia; Esti Harpeni; Hilma Putri Fidyandini
Journal of Aquatropica Asia Vol 6 No 2 (2021): Journal of Aquatropica Asia
Publisher : Jurusan Akuakultur, Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/aquatropica.v6i2.2614

Abstract

This study aims to analyze the effect of the various concentration of guava leaf extract (Psidium guajava Linnaeus) on the prevention of Motile Aeromonas Septicemia disease in common carp (Cyprinus carpio Linnaeus). In a total of 150 common carp, length 9-11 cm with a density of 10 carps/aquarium. This study used a completely randomized design through 5 treatments, each treatment has 3 replications, namely (K+) consists of without guava Psidium guajava leaf extracts and without bacteria-infection Aeromonas hydrophila, (K-) consists of without guava Psidium guajava leaf extracts with bacteria-infection Aeromonas hydrophila, (A) the application of 125 ppm guava Psidium guajava leaf extracts, (B) the application of 250 ppm guava leaf extracts, (C) the application of 500 ppm guava Psidium guajava leaf extracts for 23 days. The parameters observed total of leukocytes, differensial leukocytes, hematocrit levels, erythrocyte, hemoglobin, phagocytic activity (AF), phagocytic index (IF), relative percent survival (RPS), survival rate (SR) and water quality. Data were analyzed with Anova 95% confidence interval and continued with Duncan test. The parameter also showed a significant result (P<0,05) with the application of guava Psidium guajava leaf extracts to the total leukocytes, differensial leukocytes, erythrocyte, hemoglobin, phagocytic activity, phagocytic index and relative percent survival. The results of this research indicate the concentration of 250 ppm guava Psidium guajava leaf extracts is the optimum application to increase common carp non-spesific immune responses.
Co-Authors Agung Prasetyo Agus Setyawan Agus Setyawan Agus Setyawan Elba Dwiki Esti Harpen Esti Harpeni Hilma Nahwa Firdausi Hilma Putri Fidyandini Joshua Sahat H Sitohang Limin Santoso Lisa Silviana Mas Achmad Syamsul Arif Rissa Amelia Sefi Desfeni Mawati Siti Hudaidah Supono Supono Yudha Trinoegraha Adiputra