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All Journal ACTA VETERINARIA INDONESIANA Global Medical and Health Communication Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Jurnal Integrasi Kesehatan dan Sains Jurnal Riset Kedokteran Bandung Conference Series : Medical Science
Lelly Yuniarti
Bagian Biologi Medik Dan Histologi, Fakultas Kedokteran, Universitas Islam Bandung, Bandung
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Hubungan Derajat Nyeri dan Klasifikasi Radiologik dengan Kualitas Hidup Pasien Osteoartritis Lutut Salma Nur Afina; Lelly Yuniarti; Sadeli Masria; Hilmi Sulaiman Rathomi; Susanti Dharmmika
Jurnal Integrasi Kesehatan dan Sains Vol 1, No 2 (2019): Jurnal Integrasi Kesehatan dan Sains
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jiks.v1i2.4351

Abstract

Osteoartritis (OA) lutut merupakan masalah kesehatan masyarakat utama yang mengakibatkan nyeri dan disabilitas. Sampai saat ini masih belum jelas diketahui dampak penyakit OA lutut dilihat dari derajat nyeri dan klasifikasi radiologik OA dengan kualitas hidup pasien OA lutut. Tujuan penelitian ini adalah mengetahui hubungan derajat nyeri dan klasifikasi radiologik OA dengan kualitas hidup pasien OA lutut. Penelitian ini adalah studi analitik dengan pendekatan cross sectional. Sebanyak 85 pasien OA telah dipilih secara consecutive dan dinilai kualitas hidupnya yang terdiri atas delapan komponen menggunakan kuesioner SF-36, derajat nyerinya menggunakan numeric rating scale (NRS), serta secara radiologik berdasar atas klasifikasi Kellgren-Lawrence (KL). Penelitian ini dilakukan di RS Al-Islam Bandung selama periode April–Juli 2018. Data dianalisis dengan SPSS melalui Uji Kruskal-Wallis. Dari hasil penelitian didapatkan mayoritas pasien OA lutut mengalami derajat nyeri sedang (50%) termasuk dalam klasifikasi radiologik OA moderat (43%) dan skor kualitas hidup 548 (265,63–728,5). Penelitian ini menunjukkan hubungan bermakna antara derajat nyeri dan kualitas hidup pada aspek nyeri dan vitalitas (p=0,000 dan 0,005) serta hubungan klasifikasi radiologik OA dengan kualitas hidup aspek fungsi sosial (p=0,027). Disimpulkan bahwa terdapat hubungan derajat nyeri dengan kualitas hidup pada aspek nyeri dan vitalitas serta hubungan klasifikasi radiologik OA dengan kualitas hidup pada aspek fungsi sosial.RADIOGRAPHIC CLASSIFICATION WITH QUALITY OF LIFE IN KNEE OSTEOARTHRITIS PATIENTKnee osteoarthritis (OA) is a major public health issue causing chronic pain and disability. Until now there is a little explanation on the impact of degree of pain and radiographic classification of OA on quality of life (QOL) in knee OA patient. The objective of this study was to clarify association between degree of pain and radiographic classification of OA with QOL in knee OA patient. A total of 85 knee OA patients were selected consecutively and assessed for their eight component of quality of life using the SF-36 questionnaire, their degree of pain using numeric rating scale (NRS), and radiologically based on the Kellgren-Lawrence (KL) classification. This research was conducted in RS Al-Islam Bandung during period of April–July 2018. Data were analyzed with SPSS through Kruskal-Wallis test. From the results of the study, the majority of OA patients experienced moderate pain degree (50%) and were included in moderate OA classification (43%) and QOL score of 548 (265.63–728.5). This study showed that there was a significant association between degree of pain and QOL on pain and vitality component (p=0.000 and 0.005) and association between OA radiographic classification and QOL in social functioning component (p=0.027). It can be concluded that there is association between degree of pain with QOL on pain and vitality component also association between radiologic classification of OA with QOL on social functioning component in knee OA patient.
RANCANGAN PRIMER SPESIFIK GEN MACROPHAGE MANNOSE RECEPTOR (MMR) UNTUK POLYMERASE CHAIN REACTION (PCR) DAN SEKUENSING DEOXYRIBO NUCLEIC ACID (DNA) Yani Triyani; Nurizzatun Nafsi; Lelly Yuniarti; Nanan Sekarwana; Endang Sutedja; Dida Ahmad Gurnida; Ida Parwati; Bachti Alisjahbana
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 22, No 2 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v22i2.1120

Abstract

The order (sequencing) determinationof DeoxyribonucleicAcid (DNA) bases is the gene’s most basic information, using the methodof Polymerase Chain Reaction (PCR) as its stage. A key factor of successful detection by PCR is specific PCR primer design choice. Thedetection of diversity of Mycobacterium Mannose Receptor (MMR) gene, responsible for recognizing mannosylated antigen structureof Mycobacterium tuberculosis (M.tb) by DNA sequencing of exon 7 chromosome 10p12, related to susceptiblity for PulmonaryTuberculosis(TB), was first performed in China in 2012. The purpose of this study was to find specific primerfromboth design originatedfrom the research in China/primer I and my own design/primer IIby using Primer3 software. This study was based on 10 healthy subjectsand was a preliminary study of a research titled. The Relationship of Single Nucleotide Polymorphisms (SNPs) of Macrophage MannoseReceptor Gene to Pulmonary Tuberculosis Cases. The examination materials consist of 3 mL of EDTA blood and DNA extraction from itsbuffy coat. The resulting DNA was processed by PCR to amplify MMR gene with primer I and II. The primer I successfully amplified DNAfragments up to 780bp while primer II only 329 bp. The MMR gene DNA sequencing analysis was performed on the amplification resultof both kinds primers by using DNA Baser and Ensembl−BLAST software. The results were different, DNA sequencing result by using theprimer I was found in several chromosomes and also in several loci. Whereas, by using the primer II, it was only found in chromosome10 and in the same locus. Based on this study, it can be concluded that the specific primer design is one of the most important factorsin the success of DNA sequencing.
Hubungan Rasio CT dan Ekspresi Gen E dengan Kejadian Gagal Napas pada Pasien Covid-19 Rawat Inap di RS X Dika Rifky Fernanda; Lelly Yuniarti
Jurnal Riset Kedokteran Volume 1, No.2, Desember 2021, Jurnal Riset Kedokteran (JRK)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.238 KB) | DOI: 10.29313/jrk.v1i2.563

Abstract

Abstract. Introduction: Corona virus disease 2019 (COVID-19) is a new disease that causes millions of deaths worldwide. The majority of deaths are due to pulmonary degeneration leading to respiratory failure. The gold standard for COVID-19 diagnostics is the examination of the nucleic acid of the SARS-CoV-2 virus using RT-PCR which produces CT values for the SARS-CoV-2 gene target and internal control (CT). One of the target genes that is often examined in COVID-19 examination is gene E. This study aims to analyze the relationship between the CT IC/CT gene E ratio and the relative expression of the SARS-CoV-2 E gene with the incidence of respiratory failure. Methods: This study is a cross sectional study conducted at X General Hospital on 196 patients. Data obtained from medical records in the form of patient characteristics and PCR examination results from the laboratory. Relative gene expression was calculated using the livak method (2-ΔΔCT). The CT ratio was calculated by comparing the internal control CT with the CT gene E. The relationship of gene relative expression and CT ratio with the incidence of respiratory failure was analyzed using thestatistic unpaired t-test at 95% confidence level. Results: The results showed that the characteristics of COVID-19 patients were more males, aged 41-60 and 40 of the total patients experienced pulmonary distress. The unpaired t-test showed that there was no relationship between the CT IC/CT gene E ratio and the relative expression of the SARS-CoV-2 gene E and the incidence of respiratory failure, with P values (P=0.2083) and (P=0,8018). Conclusion: The conclusion of this study is that there is no relationship between the ratio of internal control CT/CT of the SARS-CoV-2 gene and the relative expression level of the SARS-CoV-2 E gene with the incidence of respiratory failure in confirmed COVID-19 patients hospitalized at Hospital X. There are several factors that influence the incidence of respiratory failure in addition to comorbidities such as age and gender. Abstrak. Pendahuluan: Corona virus disease 2019 (COVID-19) adalah penyakit baru yang menyebabkan jutaan kematian di seluruh dunia. Mayoritas kematian disebabkan oleh perburukan paru yang berujung pada gagal napas. Standar baku emas diagnostik COVID-19 adalah pemeriksaan asam nukleat virus SARS-CoV-2 dengan menggunakan RT-PCR yang menghasilkan nilai CT target gen SARS-CoV-2 dan internal control (CT). Salah satu gen target yang sering diperiksa pada pemeriksaan COVID-19 adalah gen E. Penelitian ini bertujuan untuk menganalisis hubungan rasio CT IC/CT gen E dan ekspresi relatif gen E SARS-CoV-2 dengan kejadian gagal napas. Metode: Penelitian ini merupakan penelitian cross sectional yang dilakukan di RS X pada 196 pasien. Data diperoleh dari medical record berupa karakteristik pasien dan hasil pemeriksaan PCR dari laboratorium. Ekspresi gen relatif dihitung dengan menggunakan metode livak (2-ΔΔCT). Rasio CT dihitung dengan membandingkan CT internal control dengan CT gen E. Hubungan ekspresi relatif gen dan rasio CT dengan kejadian gagal napas dianalisis menggunakan uji statistik unpaired t-test pada tingkat kepercayaan 95%. Hasil: Hasil penelitian didapatkan karakteristik pasien COVID-19 lebih banyak laki-laki, usia 41−60 dan 40 orang dari total pasien mengalami pulmonary distress. Uji unpaired t-test menujukan tidak terdapat hubungan antara rasio CT IC/CT gen E dan ekspresi relatif gen E SARS-CoV-2 dengan kejadian gagal napas, dengan nilai P berturut-turut P=0,2083 dan P=0,8018. Kesimpulan: Kesimpulan penelitian ini adalah tidak terdapat hubungan antara rasio CT internal control/CT gen E SARS-CoV-2 dan tingkat ekspresi relatif gen E SARS-CoV-2 dengan kejadian gagal napas pada pasien terkonfirmasi COVID-19 yang dirawat inap di RS X. Terdapat beberapa faktor yang memengaruhi kejadian gagal napas selain komorbid seperti usia dan jenis kelamin.
Rasio Cycle Threshold (CT) Internal Control (IC)/CT Gen N Sars-Cov-2 dan Ekspresi Gen N Sars-Cov-2 Berhubungan dengan Tingkat Keparahan Pasien Covid-19 Gibran Bramasta Dirgavansya; Umar Islami; Lelly Yuniarti
Bandung Conference Series: Medical Science Vol. 2 No. 1 (2022): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v2i1.1212

Abstract

Abstract. Introduction: COVID-19 is a disease caused by a virus called SARS-CoV-2, COVID-19 cases in Indonesia reached 4.2 million in October 2021. The standard for diagnosis of COVID-19 is RT-PCR because of its high specificity and sensitivity, RT -PCR produces a CT value that can describe the amount of nucleic acid and viral load of SARS-CoV-2. This study aims to analyze the relationship between the expression of SARS-CoV-2 gene N and the ratio of CT internal control/CT gene N SARS-CoV-2 with the severity of illness in COVID-19 patients. Methods: This study was an observational study with a cross-sectional approach to inpatients at Cideres Majalengka Hospital, the data was taken in the form of medical records and the results of the RT-PCR examination were CT values. The expression of the SARS-CoV-2 N gene was calculated using the saliva formula (Formula 2-ΔΔCT), while the ratio was obtained by comparing the CT values internal control /CT gene N SARS-CoV-2. The relationship of gene expression and CT ratio with severity was analyzed using the correlation test Spearman at a 95% confidence level. Results: The correlation test Spearman showed a relationship between the expression of SARS-CoV-2 Gene N (r=0.31; p=0.015) and the ratio of CT internal control/CT gene N SARS-CoV-2 (r= 0.28; p = 0.03) with the severity of COVID-19 patients. Severity is classified according to the COVID-19 Management Guidelines into mild, moderate, severe, critical. Conclusion: The conclusion of this study is that there is a relationship between the expression of SARS-CoV-2 gene N and the ratio of CT internal control/CT gene N SARS-CoV-2 with the severity of COVID-19 patients, the greater the gene expression ratio value, the severity of the patient Heavier. The ratio of CT internal control /CT gene N SARS-CoV-2 and high gene expression indicate a high viral load that can increase the severity of the patient. Abstrak. Pendahuluan: COVID-19 adalah penyakit yang diakibatkan oleh virus bernama SARS-CoV-2, kasus COVID-19 di Indonesia mencapai 4,2 juta pada Oktober 2021. Standar baku diagnosis COVID-19 adalah RT-PCR karena spesifisitas dan sensitivitasnya tinggi, RT-PCR menghasilkan CT value yang dapat menggambarkan jumlah asam nukleat dan viral load SARS-CoV-2. Penelitian ini bertujuan menganalisis hubungan ekspresi gen N SARS-CoV-2 dan rasio CT internal control/CT gen N SARS-CoV-2 dengan tingkat keparahan penyakit pasien COVID-19. Metode: Penelitian ini merupakan penelitian observasional dengan pendekatan potong lintang pada pasien rawat inap RSUD Cideres Majalengka, data diambil berupa rekam medik dan hasil pemeriksaan RT-PCR berupa CT value. Ekspresi gen N SARS-CoV-2 dihitung menggunakan rumus Livak (Rumus 2-ΔΔCT), sementara rasio didapatkan dengan membandingkan nilai CT internal control/CT gen N SARS-CoV-2. Hubungan dari ekspresi gen dan rasio CT dengan tingkat keparahan dianalisa dengan menggunakan uji korelasi spearman pada tingkat kepercayaan 95%. Hasil: Uji korelasi spearman menunjukkan adanya hubungan antara ekspresi gen N SARS-CoV-2 (r=0,31; p=0,015) dan rasio CT internal control/CT gen N SARS-CoV-2 (r= 0,28; p=0,03) dengan tingkat keparahan pasien COVID-19. Tingkat keparahan diklasifikasikan berdasar Pedoman Tatalaksana COVID-19 menjadi ringan, sedang, berat, kritis. Kesimpulan: Kesimpulan dari penelitian ini adalah terdapat hubungan antara ekspresi gen N SARS-CoV-2 dan rasio CT internal control/CT gen N SARS-CoV-2 dengan tingkat keparahan pasien COVID-19, semakin besar nilai ekspresi gen rasio maka tingkat keparahan pasien semakin berat. Rasio CT internal control/CT gen N SARS-CoV-2 dan ekspresi gen yang tinggi menunjukkan viral load yang tinggi sehingga dapat meningkatkan tingkat keparahan pasien.
Analisis In Sillico Mekanisme Cell Survival Zat Aktif Daun Sirsak (Annona Muricata Linn) pada Kanker Kolorektal Hur'iynazzahra Kariima Romli; Lelly Yuniarti; Julia Hartati
Bandung Conference Series: Medical Science Vol. 2 No. 1 (2022): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v2i1.1316

Abstract

Abstract. Colorectal cancer (CRC) is a malignancy of the colon which is the second leading cause of cancer death. The first-line treatment for CRC consists of chemotherapy and monoclonal antibodies that target cell survival. The efficacy of chemotherapy drugs in most cases of CRC still needs to be improved because the cancer cells show that they still have a defense mechanism. In several studies, the active compounds of soursop leaves have several anticancer effects such as cell survival. The drug discovery process requires time, several stages of clinical trials, and large funds so we need a method that can help make them more efficient, one of which is in sillico method. This study used the in sillico method by looking for the active compound of soursop leaves from published scientific articles and also looking for three-dimensional structures, predicting, and analysis of target proteins using several databases. The results of this study found 18 target proteins of soursop leaf active compound is involved in cell survival mechanism of CRC by regulating cell cycle regulation, miRNA transcription regulation, decreasing cell proliferation, triggering apoptosis, decreasing cell invasion and metastasis. The conclusion of this study is that the active substances of soursop leaves have some target proteins that can have some anticancer mechanism, one of which is by suppressing cell survival in CRC. Abstrak. Kanker kolorektal (KKR) merupakan keganasan pada usus besar yang menjadi kanker kedua penyebab kematian tertinggi akibat kanker. Pengobatan lini pertama KKR diantaranya kemoterapi dan antibodi monoklonal yang memiliki target salah satunya mekanisme cell survival. Efikasi obat kemoterapi pada sebagian besar kasus KKR masih perlu ditingkatkan karena sel kanker menunjukkan masih memiliki mekanisme pertahanan. Pada beberapa penelitian, zat aktif daun sirsak memiliki efek sebagai antikanker seperti melalui cell survival. Proses penemuan obat memerlukan waktu, beberapa tahap uji klinis, dan dana yang besar sehingga perlu metode yang membantu agar lebih efisien, salah satunya in sillico. Penelitian ini menggunakan metode in sillico dengan melakukan penelusuran zat aktif daun sirsak dari artikel ilmiah yang terpublikasi dan dilakukan pencarian stuktur tiga dimensi, prediksi, serta analisis protein target menggunakan beberapa database. Hasil dari penelitian ini yaitu ditemukan 18 protein target zat aktif daun sirsak yang terlibat pada mekanisme cell survival KKR dengan mengatur regulasi siklus sel, regulasi transkripsi miRNA, memicu apoptosis, menurunkan proliferasi sel, kemampuan invasi, dan metastasis sel. Kesimpulan pada penelitian ini yaitu zat aktif daun sirsak memiliki protein target yang dapat memiliki mekanisme antikanker salah satunya dengan menekan cell survival pada KKR.
Systematic Review: Efektivitas Siprofloksasin Topikal pada Pengobatan Otitis Media Supuratif Kronik Anissha Fitry Oktavianita; Lelly Yuniarti
Jurnal Riset Kedokteran Volume 2, No.2, Desember 2022, Jurnal Riset Kedokteran (JRK)
Publisher : UPT Publikasi Ilmiah Unisba

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/jrk.vi.1506

Abstract

Abstract. Chronic suppurative otitis media or CSOM is an chronic inflammatory process that occurs in the middle ear, which is caused by bacteria with perforation of the tympanic membrane and the presence of ottorhoea. The most common causative bacteria is Pseudomonas aeruginosa. Topical antibiotic using ciprofloxacin can be used to treat for CSOM. Several studies have shown topical antibiotic to be more effective than systemic antibiotics. The aim of this study was to analyze the effectiveness of ciprofloxacin in the treatment of chronic suppurative otitis media. The study was conducted using systematic review method and database used in this study were Google Scholar, Science Direct, Proquest, Springer Link, Cochrane Library, and EBSCO HOST with 599 articles of journals obtained. The screening result on articles in accordance with the inclusion citeria were 344 articles and the exclusion criteria were 340 articles. The results of critical appraisal as many 4 articles.The study was conducted during March–December 2020. The results of review dan analysis from 4 articles of each study, topical ciprofloxacin was more effective topical neomycin, framycetin gramicidin-dexamethasone (FGD), and boric acid in the treatment of chronic suppurative otitis media. The parameters for relieving of symptomatic symptoms, perforation of tympanic membrane, and hearing quality. The conclusion of this study is that ciprofloxacin topical is more effective in the treatment of chronic suppurative otitis media. Abstrak. Otitis media supuratif kronik atau OMSK merupakan proses inflamasi kronik yang terjadi pada telinga bagian tengah yang diakibatkan oleh bakteri, ditandai dengan perforasi membran timpani serta ottorhoea yang terjadi secara terus menerus. Bakteri penyebab paling umum adalah Pseudomonas aeruginosa. Pengobatan OMSK dapat menggunakan antibiotik topikal siprofloksasin. Beberapa penelitian menunjukkan antibiotik topikal lebih efektif dibanding dengan antibiotik sistemik. Tujuan peneltian ini adalah menganalisis efektivitas siprofloksasin topikal pada pengobatan otitis media supuratif kronik. Penelitian ini merupakan systematic review dengan database yang digunakan adalah Google Scholar, Science Direct, Proquest, Springer Link, Cochrane Library, dan EBSCO HOST dengan jumlah artikel yang didapat sebanyak 599 artikel. Hasil skrining pada artikel yang sesuai dengan kriteria inklusi sebanyak 344 artikel dan kriteria eksklusi sebanyak 340 artikel. Setelah dilakukan telaah kritis, artikel yang di-review sebanyak empat artikel. Penelitian dilakukan pada periode Maret–Desember 2020. Hasil telaah dan analisis dari empat artikel menunjukkan bahwa siproflokasin topikal lebih efektif dibanding dengan topikal neomisin, framisetin gramisidin-deksametason (FGD), dan asam borik pada pengobatan otitis media supuratif kronik. Parameter kesembuhan gejala ottorhoea, perforasi membran timpani, serta kualitas pendengaran. Kesimpulan penelitian ini adalah siprofloksasin topikal efektif pada pengobatan otitis media supuratif kronik.
Hubungan Komorbid Hipertensi dengan Gejala Long Covid-19: Studi Pada Pasien Covid-19 yang Pernah di Rawat Inap di RSUD Al-Ihsan Provinsi Jawa Barat dan RSUD Cideres Majalengka Althaafvashti Trixie Niadi; Lelly Yuniarti; Abdul Hadi Hassan
Bandung Conference Series: Medical Science Vol. 3 No. 1 (2023): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v3i1.6267

Abstract

Abstract. Long COVID-19 is a prolonged symptom in patients who have recovered from COVID-19 which incidence increases along with the increasing cases of COVID-19. One of the risk factors for long COVID-19 is comorbid hypertension. This study aimed to discover the relationship between comorbid hypertension and long COVID-19 symptoms. The subjects of this study were COVID-19 inpatients at Al-Ihsan and Cideres Hospital from December 2020–February 2022. This analytical study used a case-control design conducted from December 2021–December 2022. Primary data was gained through interviews and questionnaires. Secondary data was acquired from medical records. The total subjects were 120 patients (65 subjects as cases and 55 subjects as controls) who met the inclusion criteria and were selected using the purposive sampling technique. The results showed that most COVID-19 patients aged 56–65 years and the number was comparable between men and women, most long COVID-19 patients aged 56–65 years and are female, more subjects did not have comorbid hypertension (54.2%), more subjects experienced long COVID- 19 symptoms (54.2%), and the most common long COVID-19 symptoms were fatigue, coughing, and dyspnea. Most COVID-19 patients with comorbid hypertension (41.7%) experienced long COVID-19 symptoms, while most COVID- 19 patients without comorbid hypertension did not experience long COVID-19 symptoms. The conclusion of this study, based on bivariate analysis using Fisher’s exact test with a 95% confidence level, is that there is a correlation between comorbid hypertension and long COVID-19 symptoms (p<0.05). COVID-19 patients with comorbid hypertension are more at risk of experiencing long COVID-19 symptoms. Abstrak. Long COVID-19 merupakan gejala berkepanjangan pada pasien yang telah pulih dari COVID-19 yang kejadiannya meningkat seiring dengan meningkatnya kasus COVID-19. Salah satu faktor risiko terjadinya long COVID-19 adalah komorbid hipertensi. Tujuan penelitian ini adalah mengetahui hubungan komorbid hipertensi dengan gejala long COVID-19. Subjek penelitian adalah pasien COVID- 19 yang pernah dirawat inap di RSUD Al-Ihsan dan Cideres pada Desember 2020– Februari 2021. Penelitian analitis ini menggunakan desain case-control dan dilaksanakan pada Desember 2021–Desember 2022. Data primer diperoleh melalui wawancara dan pengisian kuesioner serta data sekunder diperoleh dari rekam medis. Total subjek penelitian berjumlah 120 pasien yaitu didapatkan 65 kasus dan 55 kontrol yang memenuhi kriteria inklusi dan dipilih dengan teknik purposive sampling. Hasil penelitian menunjukkan bahwa pasien COVID-19 paling banyak berusia 56–65 tahun dan sebanding jumlahnya antara laki-laki dan perempuan, pasien long COVID-19 paling banyak berusia 56–65 tahun dan lebih banyak yang berjenis kelamin perempuan, lebih banyak pasien COVID-19 yang tidak memiliki komorbid hipertensi (54,2%), lebih banyak pasien COVID-19 yang mengalami gejala long COVID-19 (54,2%), dan gejala long COVID-19 yang paling sering dialami adalah fatigue, batuk, dan dyspnea. Sebagian besar pasien COVID-19 dengan komorbid hipertensi (41,7%) mengalami gejala long COVID-19, sedangkan sebagian besar pasien COVID-19 tanpa komorbid hipertensi tidak mengalami gejala long COVID- 19. Kesimpulan penelitian ini, berdasarkan analisis bivariat menggunakan Fisher’s exact test dengan tingkat kepercayaan 95%, adalah terdapat hubungan antara komorbid hipertensi dan gejala long COVID-19 (p<0,05). Pasien COVID-19 dengan komorbid hipertensi lebih berisiko mengalami gejala long COVID-19.
Efek Antikanker dan Ko-Kemoterapi Nanopartikel Alginat Ekstrak Etanol Daun Sirsak (Annona Muricata) dengan Doxorubicin pada Kultur Sel Kanker Serviks Uteri (HeLa) Revina Mayra Salsabila Hasyim; Lelly Yuniarti; Yuke Andriane
Bandung Conference Series: Medical Science Vol. 3 No. 1 (2023): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v3i1.5741

Abstract

Abstract. Uterine cervical cancer ranks fourth in the world and Asia and second in Indonesia. Cervical uteri cancer chemotherapy with doxorubicin still has high side effects. To reduce these side effects, combination therapy can be carried out between standard drugs and drugs from natural ingredients, one of which is soursop leaf in the form of nanoparticles which can increase the delivery of compounds. The purpose of this study was to analyze the anticancer effect of alginate nanoparticles of soursop leaf ethanol extract and its combination with doxorubicin on cervical uteri cancer (HeLa) cells. This research is a pure in vitro experimental study on cervical uteri cancer cell culture (HeLa). The cytotoxicity test was carried out using the microtetrazolium method and analyzed using the SPSS software probit regression. The combination test was carried out with concentrations of 1/2 IC50, 3/8 IC50, 1/4 IC50, 1/8 IC50 and analyzed using CompuSyn software to obtain a CI value indicating the synergy of the preparations. This study had an IC50 of soursop leaf extract nanoparticles of 52.0825 (±24.26) µg/mL (moderate effect) and doxorubicin which had an IC50 of 4.8 (±0.10) µg/mL (strong effect). The lowest CI value obtained was 0.04106. The conclusion of this study is that soursop leaf extract nanoparticles have a moderate anticancer effect and are highly synergistic with eight combinations of doxorubicin. Abstrak. Kanker serviks uteri menempati urutan keempat di dunia dan Asia serta kedua di Indonesia. Kemoterapi kanker serviks uteri dengan doxorubicin masih memiliki efek samping yang tinggi. Untuk menurunkan efek samping tersebut, dapat dilakukan terapi kombinasi antara obat standar dengan obat dari bahan alam, salah satunya adalah daun sirsak dalam bentuk nanopartikel yang dapat meningkatkan penghantaran senyawa. Tujuan penelitian ini adalah untuk menganalisis efek antikanker sediaan nanopartikel alginat ekstrak etanol daun sirsak dan kombinasinya dengan doxorubicin pada sel kanker serviks uteri (HeLa). Penelitian ini merupakan penelitian eksperimental murni in vitro pada kultur sel kanker serviks uteri (HeLa). Uji sitotoksisitas dilakukan menggunakan metode microtetrazolium dan dianalisis melalui regresi probit software SPSS. Uji kombinasi dilakukan dengan konsentrasi 1/2 IC50, 3/8 IC50, 1/4 IC50, 1/8 IC50 dan dianalisis menggunakan software CompuSyn hingga didapatkan nilai CI yang menunjukkan sinergitas sediaan. Penelitian ini memiliki hasil IC50 nanopartikel ekstrak daun sirsak sebesar 52,0825 (±24,26) µg/mL (efek sedang) dan doxorubicin yang memiliki IC50 sebesar 4,8 (±0,10) µg/mL (efek kuat). Nilai CI terendah yang didapatkan adalah sebesar 0,04106. Kesimpulan penelitian ini adalah nanopartikel ekstrak daun sirsak memiliki efek antikanker sedang dan bersifat sinergis sangat kuat dengan doxorubicin sebanyak delapan kombinasi.
Terdapat Hubungan Antara Jenis dan Frekuensi Olahraga dengan Tingkat Stres pada Mahasiswa Kedokteran di Bandung Azmi Putri Azzahra; Lelly Yuniarti; Julia Hartati
Bandung Conference Series: Medical Science Vol. 3 No. 1 (2023): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v3i1.6934

Abstract

Abstract. Stress is one of the physiological reactions in an individual where various defense mechanisms occur to face situations that are considered threatening or increased demands. One of the ways to reduce stress levels is to exercise. Exercise will stimulate the production of beta-endorphin hormones which will have the effect of restoring mood and lowering cortisol levels. This research aims to analyze the relationship between the type and frequency of sports and the stress level of Faculty of Medicine students in Bandung. The research method used is descriptive comparative with cross-sectional. The subjects of this research are 135 students of the Faculty of Medicine in the City of Bandung, with simple random sampling. The type and frequency of sports were taken using the Godin Leisure-Time Exercise questionnaire, while the stress level was assessed using the Perceived Stress Scale. Data were analyzed with Fisher's Exact Test with a confidence level of 95% (α=0,05). The results of the research on the relationship between the type of sport and the level of stress obtained p=0.024 and the relationship between the frequency of sport and the level of stress obtained p=0.004. The conclusion of this research is that there is a relationship between the type and frequency of sports and the level of stress. Abstrak. Stres merupakan salah satu reaksi fisiologis pada suatu individu di mana terjadi mekanisme pertahanan yang beragam untuk menghadapi situasi yang dianggap mengancam atau tuntutan yang meningkat. Salah satu cara untuk menurunkan tingkat stres adalah berolahraga. Olahraga akan merangsang pengeluaran hormon beta-endorfin yang akan memberikan efek memulihkan suasana hati dan menurunkan kadar kortisol. Penelitian ini bertujuan untuk menganalisis hubungan jenis dan frekuensi olahraga dengan tingkat stres Mahasiswa Fakultas Kedokteran di Bandung. Metode penelitian yang digunakan adalah deskriptif comparative dengan cross-sectional. Subjek pada penelitian ini adalah Mahasiswa Fakultas Kedokteran di Kota Bandung berjumlah 135 orang, dengan pengambilan sampel secara simple random sampling. Jenis dan frekuensi olahraga diambil menggunakan kuisioner Godin Leisure-Time Exercise sedangkan tingkat stres dinilai menggunakan Perceived Stres Scale. Data dianalisis dengan uji Fisher’s Exact Test dengan tingkat kepercayaan 95% (α=0,05). Hasil penelitian hubungan jenis olahraga dengan tingkat stres didapatkan p=0.024 dan hubungan frekuensi olahraga dengan tingkat stres didapatkan p=0.004. Simpulan pada penelitian ini terdapat hubungan jenis dan frekuensi olahraga dengan tingkat stres.
Komorbid Diabetes Melitus Bukan Merupakan Faktor Risiko Long COVID-19 Muhamad Rizky Pratama Kurniawan Rizky; Lelly Yuniarti Lelly; Umar Islami Umar
Bandung Conference Series: Medical Science Vol. 3 No. 1 (2023): Bandung Conference Series: Medical Science
Publisher : UNISBA Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29313/bcsms.v3i1.6220

Abstract

Abstract. As many as 30% of COVID-19 patients have comorbid diabetes mellitus. Diabetes is suspected to cause worsening of symptoms and a prolonged duration of symptoms known as long COVID-19. The high number of COVID-19 cases in Indonesia will probably be directly proportional to the long incidence of COVID-19, in Indonesia there are as many as 63.5% of COVID-19 patients experiencing symptoms of long COVID-19. The purpose of this study was to analyze the relationship between comorbid diabetes mellitus and symptoms of long COVID-19. The method used in this research is case control analysis. The research subjects were 91 COVID-19 patients who had been hospitalized at SMC Tasikmalaya Hospital and Cideres Majalengka Hospital and had been declared cured, selected by purposive sampling technique. Processing data using fisher's exact test. The results showed that 50 subjects were male and 41 subjects were female. 51 subjects had a history of comorbid diabetes mellitus. There were 46 subjects experiencing symptoms of long COVID-19 with the most age range experiencing symptoms of long COVID-19 at the age of 41-60 years as many as 53 subjects and the least at age >60 years as many as 18 subjects. There was no significant relationship between co-morbid diabetes mellitus and symptoms of long COVID-19 with a p-value of 0.240 (>0.05). It was concluded that comorbid diabetes mellitus in COVID-19 patients is not a risk factor for the appearance of long-term symptoms of COVID-19. Keywords: COVID-19, diabetes mellitus, long COVID-19
Co-Authors Abdul Hadi Hassan Agly Adithya Agly Adithya, Agly Althaafvashti Trixie Niadi Anggit Yanuaristi Anissha Fitry Oktavianita Anissha Fitry Oktavianita Apen Afgani Azmi Putri Azzahra Azri Iskandar Azri Iskandar, Azri Bachti Alisjahbana Clara Juniasari Dean Wahjudi Satyaputra Dida Ahmad Gurnida Dika Rifky Fernanda Dika Rifky Fernanda Endang Sutedja Enggar Hestu Enggar Hestu, Enggar Gibran Bramasta Dirgavansya Gibran Bramasta Dirgavansya Herri S. Sastramihardja Herry Garna Heru Haerudin Hilmi Sulaiman Rathomi Hur'iynazzahra Kariima Romli Ida Parwati Julia Hartati Julia Hartati Maya Tejasari Miranti Kania Dewi Muhamad Rizky Pratama Kurniawan Rizky Nanan Sekarwana Nurizzatun Nafsi Revina Mayra Salsabila Hasyim Sadeli Masria Salma Nur Afina Samsudin Surialaga Silmi Hizba Hunafa Siti Annisa Devi Trusda, Siti Annisa Devi Suganda Tanuwijaya Suganda Tanuwijaya, Suganda Susan Fitriyana Susanti Dharmmika Tety H. Rahim Titik Respati Gilarsi Tryando Bhatara Uci Ary Lantika Umar Islami Umar Islami Umar Wida Purbaningsih Yani Triyani Yani Triyani Yuke Andriane Yuktiana Kharisma Zulmansyah , Zulmansyah