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All Journal JEJAK BERITA BIOLOGI JURNAL BIOLOGI INDONESIA ANNALES BOGORIENSES
Satya Nugroho
Pusat Penelitian Bioteknologi LIPI Jl. Raya Bogor Km 46 Cibinong, Bogor 16
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Immunology & microbiology Medicine & Pharmacology

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PENGARUH MODAL, TENAGA KERJA DAN TEKNOLOGI TERHADAP HASIL PRODUKSI SUSU KABUPATEN BOYOLALI Nugroho, Satya; Budianto, Muchamad Joko
JEJAK: Jurnal Ekonomi dan Kebijakan Vol 7, No 2 (2014): September 2014
Publisher : Semarang State University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/jejak.v7i2.3896

Abstract

Currently, most of milk in Indonesia should be imported. It is about 78,89 %, and the rest, that is about 22,11% is from local production or local dairy farmers. However, there are some problems faced by the dairy farmers in Kecamatan Musuk. They are capital, workforces, and technology. This study aims at knowing the influence of capital, workforces, and technology to the milk production in Kecamatan Musuk. Ordinary Least Square (OLS) was applied for analizing the data. The results show that the regression coefficient values of each independent variable (the variables of capital, workforces, and technology) positively influence the production of milk. Based on the T-test (partial), the capital and workforces influence positively and significantly to the production. In addition, technology also has positive effect to the milk production, but it is not significant. Finally, based on F-test, the milk production is influenced by the capital, workforces and technology. It is 87%. Saat ini sebagian besar susu di Indonesia masih harus di impor (sekitar 78,89%), sedangkan 22,11%nya di pasok dari produksi susu domestik yang sebagian besar dihasilkan oleh peternak sapi perah rakyat. Namun ada beberapa permasalaham yang kerap dihadapi oleh peternak sapi perah di Kecamatan Musuk, baik dari segi permodalan, tenaga kerja maupun teknologi yang dipakai.Penelitian ini bertujuan untuk mengetahui seberapa besar pengaruh dari penggunaan faktor produksi modal, tenaga kerja dan teknologi terhadap hasil produksi susu sapi perah di Kecamatan Musuk. Alat analisis yang digunakan adalah analisis regresi berganda atau Ordinary Least Square (OLS).Hasil analisis diperoleh bahwa nilai koefisien regresi masing-masing variabel bebas yaitu variabel Modal, Tenaga Kerja, dan Teknologi berpengaruh secara positif terhadap produksi susu sapi perah. Dari hasil uji-t (parsial) modal dan tenaga kerja berpengaruh positif dan signifikan sedangkan teknologi berpengaruh positif namun tidak signifikan. Uji F menunjukkan produksi susu sapi perah dipengaruhi oleh modal, tenaga kerja dan teknologi sebesar 87%.
Parameter Genetik dan Seleksi Sorgum [Sorghum bicolor (L.) Moench] Populasi F4 Hasil Single Seed Descent (SSD) Sulistyowati, Yuli; koesoemaningtyas, Tri; Sopandie, Didy; Ardie, Sintho Wahyuning; Nugroho, Satya
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1035.711 KB) | DOI: 10.14203/jbi.v12i2.2878

Abstract

The objective of this study were to obtain information about genetic parameters of agronomic characters of sorghum populations derived from Single Seed Descent (SSD) method, and to estimate selection differensial and also to select of F4 population that have a high yield and medium plant height . The experiment was carried out at Leuwikopo Farm, Darmaga, Bogor from January to April 2014. The genetic material were F4 population derived from SSD. The results showed that based on the skewness and kurtosis values, all of the agronomy characters observed in this study were poligenic controlled and influenced by additive gene action. The genetic coefficient of variability values for all parameters varied from intermediate to high in F4 population. Agronomic characters in F4 responded positively to selection because of high broad sense heritability estimates. Correlation analysis showed that plant height, number of leaves, stem diameter, panicle length, panicle weight and 100 seed weight have significant and positive correlation to grain yield/plant. Differential value with selection intensity 10 % based on grain yield/plant will increase grain yield/plant 83.89 % and plant height 8.91 % in the next generation, whereas differensial value based on grain yield/plant and plant height will increase grain yield/plants 68.33 % and plant height 0.26 % in the next generation. It means that selected plant were expected increasing yield 83.89 % or 68.33 % in next generation.Keywords: heritability, cefficient genetic variability, correlation analysis, selection differential
IDENTIFIKASI GALUR-GALUR PADI MUTANINSERSI TOLERAN DAN RENTAN CEKAMAN SALINITAS BERDASARKAN KARAKTER MULTIVARIAT PERTUMBUHAN DAN BIOKIMIA PADA FASE VEGETATIF Situmorang, Apriadi; Zannati, Anky; Widyajayantie, Dwi; Nugroho, Satya
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (538.654 KB) | DOI: 10.14203/beritabiologi.v10i4.765

Abstract

Growth and biochemical characters of thirty-three Nipponbare-based rice mutant lines independently harboring activation-tag in salt stress condition were investigated. Rice cv Nipponbare wild type, IR29 and Pokkali were used as isogenic, susceptible, and tolerant cultivars, respectively. Plants were grown in Yoshida nutrient solution containing NaCI (6 g/1) as the stress treatment and in Yoshida nutrient solution without NaCI as the control. Evaluation of salt tolerance was conducted at 21 days after salinization.The results showed that there was a significant difference in growth characters among genotypes in reduction of the plant height,root length, shoot fresh weight, root fresh weight, shoot dry weight, as well as in the root dry weight. Biochemical characters of the genotypes also showed significant difference in their reduction of chlorophyll-a and chlorophyll-b concentrations,total carotenoids and proline accumulation. Based on multivariate growth and biochemical characters,T3.PMO.VI.63.5a.30.9,T3.PMO.VI.30.1a.21.1, T3.PMO.V1.63.5a.33.7F3.PUR.IX.49.1d.l.6.5,T3.PMO.VI.81.3a.4.4, F3.PUR.IX.49.1d.l.6.5,and F3.PUR.VIII.5.1f.l.4.8 were assumed as potential salt-tolerant lines, respectively, while mutant lines T3.PMO.VI.30.1a.l07.7,T3.PMO.VI.63.5a.20.6,T3.PMO.III.4.4c.7.2,andT3.PMO.VI.30.1a.51.1 were assumed as salt-susceptible lines respectively.
Genetic transformation of rice cv. Ciherang using double T-DNA vector harboring cry1Ab gene Sulistyowati, Yuli; Rachmat, Agus; Zahra, Fatimah; Rahmawati, Syamsidah; Nugroho, Satya
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.64 KB) | DOI: 10.1234/37

Abstract

Rice stem borer (Scirpophaga sp.) is considered as one of the primary pests causing yield decrease in Indonesia. Genetic engineering is considered as one of the most effective way to improve the resistance of rice plants to yellow stem borer since no resistance gene has been found in rice and its wild relatives. A double T-DNA binary vector p2TDNAcryIAb wich carry two independent T-DNA was used to transform Indonesian elite rice cv Ciherang using Agrobacterium-mediated transformation to generate transgenic rice that free from selectable marker genes. One T-DNA containing a selectable marker hygromycin phosphotransferase (hpt) gene and the other carrying the cry1Ab gene. As many as 37 putative transgenic plants from 14 embryos were generated. PCR analysis demonstrated that 22 out of 37 putative transgenic rice contain cry1Ab gene. All Ciherang rice carrying the cry1Ab gene expressing Cry1Ab protein as shown by the immunostrip assay. Further work will be carried out to determine the transgenes copy number, to select marker free transgenic rice, and to evaluate the resistance of transgenic rice against neonates larvae of yellow stem borer.Keyword: double T-DNA, transgenic rice, Cry1Ab, transformation
Gene flow from genetically modified rice to their weedy and wild relatives and its environmental consequences Estiati, Amy; Nugroho, Satya
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/32

Abstract

Rice is one of the most important food crops in the world.  Nearly half of the world population consume rice as their staple food (FAO, 2004).  With the increasing of world population, the need to provide more food supplies including rice is obvious.  Biotechnology is expected to play major roles in the improvement of crop productivity and quality. Unlike transgenic maize, eventhough many research have been done to improve rice performance by genetic engineering, transgenic rice has not been released or commercialized.  Among those research to improve rice performance are the atempt to introduce biotic and abiotic stress tolerant traits, herbicide tolerant trait and beta-carotin biosynthetic pathway.  Currently, several genetically modified rice cultivars have been and being tested on limited field trials.Keywords : Rice, stress tolerant
Phenotypic Screening of Ds transposon and Activation-tag Insertional Mutant Rice Population for Drought and Salinity Tolerant Related Traits Nugroho, Satya; Zannati, Anky; Situmorang, Apriadi; Windiastri, Vincentia Esti; Widyajayantie, Dwi; Pantouw, Carla F.; Astuti, Dwi; Indrayani, Sri; Rahmawati, Syamsidah; Mulyaningsih, Enung Sri; Santoso, Tri Joko; Trijatmiko, Kurniawan Rudi
ANNALES BOGORIENSES Vol 15, No 1 (2011): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/56

Abstract

The studies of rice genes through functional genomics are greatly facilitated by the availability of the complete genome sequences, including the complete physical map of the japonica rice cv. nipponbare. Using the Ac and Ds transposon, that are capable of transposition in various heterologous plants including the monocotyledon rice,  combined with the enhancer element for generating activation taged lines, it is possible to discover and isolate functional genes involved in various important agronomical traits; such as those involved in abiotic stress tolerance (drought, high salt) and biotic stresses diseases and pests. We have developed 1,785 first (T0) generation of mutant nipponbare rice by transposons Ac/Ds insertions containing activation-tag, generated by transformation using Agrobacterium-mediated method. Currently, we have generated approximately 1,000 stable lines with transposon Ds and activation-tag insertion ready for screening. Efficient screening methods for mutant Nipponbare rice lines have been established for agronomically important mutant traits. Among the new phenotypes related to important agronomical traits observed were drought and salt tolerant or sensitive, stunted and robust growth, variable root penetration and other interesting traits such as reduced tillering, rolled leaf and thin tiller.   Keywords: rice, insertion mutant, gene discovery, drought, salinity
Identification of Drought Tolerant Related Insertional Mutant Lines Using PEG 6000 Nugroho, Satya; Windiastri, Vincentia Esti; Widyajayanti, Dwi; Pantouw, Carla Frieda
ANNALES BOGORIENSES Vol 13, No 1 (2009): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/29

Abstract

Drought is one of the most important abiotic stresses in rice (Oryza sativa) productivity. The development of drought tolerant cultivars are therefore highly desireable. We have developed insertional mutant based on the Japonica rice cv Nipponbare rice by transposons Ac/Ds insertions containing activation-tag and gene trap. Screening of the mutant population for drought tolerant related phenotypes is of our priority.  The screening protocol based on PEG 6000 has been developed and was  being used to screen 70 mutant lines to characterize their responds to the treatment based on different parameters (number of leaf, total weight, plant height, root length and number of germinating seeds).  These characters were used to score the Degradation Index  and Vigour Index. Results showed varrying responds of the lines to the osmotic presure.  Some lines showing a good performance indicated by lower Degradation Index and higher Vigour Index have been identified.  Some inconsistencies in the performances scored by both indices were thought to be due to seed quality.   Keywords:  Oryza sativa, insertion mutant, drought, PEG 6000, Degradation Index, Vigor Index.
Isolation and Characterization of OsNAC6 cDNA from Rice (Oryza sativa L.) cv. Nipponbare, Batutegi, and Rojolele Rachmat, Agus; Nugroho, Satya; Nurdiani, Dini; Swastika, Maria; Sukma, Dewi; Aswidinnoor, Hajrial; Sudarsono, Sudarsono
ANNALES BOGORIENSES Vol 18, No 2 (2014): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1372.433 KB) | DOI: 10.1234/100

Abstract

Transcription factors have an important function in regulating gene expression and plant responses to stresses. The ERF, bZIP, WRKY, MYB, and NAC are stress inducible transcription factors. The OsNAC6 is a member of the NAC transcription factor family in rice and its expression is induced by abiotic stresses, wounding and blast disease. Characterization of OsNAC6 gene sequences would give a better undersanding on how OsNAC gene functions biologically. The objectives of this research are to isolate the OsNAC6 cDNA from Nipponbare, Batutegi, and Rojolele cultivars, to characterize their DNA sequences, and to compare their sequences to other NAC genes from other plants available in GenBank DNA databases. Isolated cDNA and sequencing of the fragments resulted in a 912 bp DNA sequences. Translation of the sequences yielded a protein consisted of 303 amino acid residue. Blast analysis of amino acid sequences indicated identity of isolated cDNA from three Indonesian rice cultivars are the OsNAC6 gene. Deduced amino acid residues from amplified cDNAs of Nipponbare, Batutegi, and Rojolele cultivars shared 100% sequence identities to rice OsNAC6 (Acc. # BAA89800), 71-100% sequence identity to a number of OsNAC protein from Oryza sativa and 63-83% sequence identity to NAC protein from other plants.
Insect Bioassay in Biosafety Containment to Select Transgenic Rice (Oryza sativa L.) Harboring Cry1B Gene Resistant to Yellow Stem Borer (Scrirpophaga incertulas Walk.) Estiati, Amy; Nena, Ade; Nugroho, Satya
ANNALES BOGORIENSES Vol 17, No 2 (2013): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.1234/77

Abstract

Development of rice varieties resistant to yellow stem borer (YSB) is very crucial.  Agrobacterium-mediated transformation of Cry1B gene under wound inducible gene promoter mpi (maize proteinase inhibitor) into a local rice variety Rojolele had been conducted. PCR analysis proved that Cry1B gene had been integrated into plant genome of 3R25 and 3R5 rice lines. Segregation analysis using PCR for Cry1B gene of the two putative transgenic rice lines at third (T2), fourth (T3), fifth (T4) and sixth (T5) generations of 3R25 and 3R5 lines proved that 3R25.7.27, 3R25.7.13.8.2, 3R25.7.13.8.6, 3R25.7.13.8.8, 3R5.26.2 and 3R5.26.5 are homozygous lines for Cry1B. Insect bioassay on three randomly picked homozygous transgenic rice lines to study the efficacy of Cry1B gene toward YSB was conducted in biosafety containment by infestingYSB larvae at first instar into 3R25.7.27, 3R25.7.13.8.6 and 3R5.26.5 transgenic rice lines, using non-transgenic Rojolele, IR64 and IR74 as susceptible controls. The results showed that the precentages of deadhearts symptoms of  3R25.7.27, 3R5.26.2 and 3R25.7.13.8.6 rice lines were lower than those of the susceptible control lines with scores of 0,1 and 0, respectively. While the scores of all three susceptible control plants were 9. The results proved that lines 3R25.7.27, 3R25.7.13.8.6 and 3R5.26.2 were categorized as resistant lines while the non-transgenic Rojolele, IR64 and IR74 were categorized as susceptible lines. The results also showed that the Cry1B gene was expressed and  produced insecticidal protein CRY1B which were active against YSB to protect rice plant toward YSB infestation
Identification and Activity of The Retrotranposon Tos17 in Indonesian Javanica Rice CV.Rojolele And Japonica Rice CV. Gajahmungkur Nugroho, Satya; Loedin, Inez HS; Ouwerkerk, Pieter BF
ANNALES BOGORIENSES Vol 11, No 1 (2007): Annales Bogorienses
Publisher : Research Center for Biotechnology - Indonesian Institute of Sciences (LIPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2649.41 KB) | DOI: 10.1234/20

Abstract

Retrotransposons arc  mobile  genetic  elements  that  transpose  via  an  RNA intermediate  that  is  reverse transcribed  before  integration  into  a  new  location  within  the  host  genome. They  are  ubiquitous  in  eukaryotic organism.  and constitute a major portion of the nuclear genome (often more than half of the total DNA) in plants .Tos17 is a  rice  endogenous  retrotransposon  on  that  has  been  studied  thoroughly. Tos17 has  been  shown  to  be  an efficient insertional mutagen and saturation mutagenesis tool for gene tagging and functional genomics in Japonicarice cv  Nipponbare.  In  lavanica rice however, the presence and activity of  Tos17 has not been described thus far. while in some Indica rice Tos17 has  been found  to be  inactive. Javanica rice, also known as tropical  Japonica rice,has many cultivars which may serve as pontetial genetic resources of great interest for breeding programmes. Here  , the presence and activities of retrotransposon Tos17 in  Javanica rice  cv Rojolele was described and compared  to those of Japonica rice and Japonica rice cv Gajahmungkur We  identified  five and theree copies of  Tos17 in Rojolele and Gajahmungkur, respeclively, with different activities.Keywords: Oryza  sativa, rice, javanica, retrotransposon, Tos17
Co-Authors Agus Rachmat Anky Zannati Apriadi Situmorang Chairunnisa Chairunnisa Dewi Sukma Didy Sopandie DINI NURDIANI Dwi Astuti Dwi Widyajayantie Enung Sri Mulyaningsih Erdayani, Eva Estiati, Amy Estiati, Amy Fatimah Zahra HAJRIAL ASWIDINNOOR Kurniawan Rudi Trijatmiko, Kurniawan Rudi Loedin, Inez HS Muchamad Joko Budianto, Muchamad Joko Nena, Ade Ouwerkerk, Pieter BF Pantouw, Carla F. Pantouw, Carla Frieda Sintho Wahyuning Ardie Sri Indrayani, Sri Sudarsono Swastika, Maria Syamsidah Rahmawati, Syamsidah TRI JOKO SANTOSO TRI KOESOEMANINGTYAS Vincentia Esti Windiastri, Vincentia Esti Widyajayanti, Dwi Yuli Sulistyowati