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BIOGROUTING: Produksi Urease dari Bakteri Laut (Oceanobacillus sp.) Pengendap Karbonat Sidratu Ainiyah; Endry Nugroho Prasetyo; Puspita Lisdiyanti; Maharani Pertiwi Koentjoro
Jurnal Sains dan Seni ITS Vol 3, No 2 (2014)
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LPPM), ITS

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.397 KB) | DOI: 10.12962/j23373520.v3i2.7004

Abstract

Biogrouting adalah teknologi yang mensimulasikan proses diagenesis yaitu transformasi butiran pasir menjadi batuan pasir (calcarinite/sandstone). Permasalahan dalam penelitian ini adalah bagaimana mengoptimasi produk urease dengan melakukan uji aktifitas, mengisolasi, mempurifikasi dan mengkarakterisasi urease serta mengaplikasikannya sebagai material grout. Uji aktifitas dan optimasi dilakukan dengan menumbuhkan isolat Oceanobacillus sp. pada 2 variasi medium (B4 urea dan B4 urin), 5 variasi pH (4-8) dan 2 variasi suhu (25°C dan 29°C). Hasil uji aktifitas dan optimasi selanjutnya dipurifikasi menggunakan ammonium sulfat (Uji Bradford) dan dicari titik isoelektriknya. Kemudian hasil protein presipitat dikarakterisasi menggunakan SDS-PAGE. Berdasarkan hasil penelitian diketahui bahwa aktifitas urease paling tinggi adalah 70.21 unit/ml. Urease optimal dihasilkan pada isolat yang ditumbuhkan pada B4 urea pada pH 7 temperatur 25°C. Berat molekul urease yang dikarakterisasi menggunakan SDS-PAGE adalah 440 kDa, sedangkan titik isoelektriknya pada pH 6. Urease dapat dijadikan material grout karena memiliki kemampuan untuk melakukan sementasi pada aplikasi sederhana biogrouting.
PROFESIONALISME GURU SD NEGERI SE-KECAMATAN WARUNGASEM KABUPATEN BATANG Suatu Tinjauan Aspek Persepsi Guru tentang Kepemimpinan Kepala Sekolah dan Motivasi Berprestasi Guru Nugroho, Setyo
Jurnal Varidika: Varia Pendidikan Volume 24 No. 2, Desember 2012
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

The purposes of this study were: to analyze the influence of teacher perceptions of principal leadership to teacher profesionlisme, 2) to analyze the influence of achievement motivation to teacher, and 3) to analyze the influence of teacher perceptions of principal leadership and teacher achievement motivation teacher profesionlisme Elementary School District as Batang Warungasem. The quantitative approach is used in this research with population consists of all elementary school teachers as Warungasem Batang district with the status of civil servants, amounting to 190 people. The Sample are 148 people. Data collection method in this study is conducted through a questionnaire. The validity and reliability instrument have been test and that result is significant on 5%. The results showed that: 1) the teachers’ perceptions of principal leadership have a positive and significant effect to the teacher professionalism at 5%, 2) the teachers achievement motivation have a positive and significant efect to the teacher professionalism at 5% and 3) the teacher perceptions of principal leadership and teacher achievement motivation have a positive and significant effect on teacher professionalism elementary School District as Warungasem Batang at 5% significance level.
STUDI EKSPERIMEN MULTISILINDER VORTEX INDUCED VIBRATION ENERGY CONVERTER (VIVEC) Yuliatmoko, Bagus Albaranu; Nugroho, Setyo; Dewanto, Raden Sanggar
Jurnal Teknologi Vol 11 No 2 (2018): Jurnal Teknologi
Publisher : Jurnal Teknologi, Fakultas Teknologi Industri, Institut Sains & Teknologi AKPRIND Yogyakarta

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Abstract

INTISARI Salah satu metode pemanfaatan energi air adalah dengan metode hydrokinetic. Vortex-Induced Vibration Energy Converter (VIVEC) merupakan sebuah alat konversi energi yang memanfaatkan energi hydrokinetic. VIVEC mengkonversi gerak translasi / osilasi sebuah silinder akibat Vortex Induced Vibration (VIV) dari pelepasan vortex yang terbentuk disekitar silinder, ketika silinder ditabrak oleh aliran fluida dengan kecepatan tertentu. Gaya lift yang ditimbulkan oleh VIV membuat silinder mengalami vibrasi cross-flow sehingga menghasilkan energi kinetik. Ada beberapa parameter yang dapat meningkatkan performa dari VIVEC, salah satunya adalah penambahan jumlah silinder atau penggunaan multisilinder pada VIVEC. Dengan penggunaan sistem multisilinder, tentunya jarak antar silinder perlu diperhatikan karena vortex yang terbentuk dari salah satu silinder akan mempengaruhi frekuensi osilasi silinder lain dibelakangnya. Pada penelitian ini dirancang sebuah multisilinder VIVEC dengan konfigurasi 2x2 dengan variasi jarak (d/D = 2,4, dan 6). Pengujian secara eksperimen dilakukan dengan kecepatan fluida 0,67 m/s (Re=50.000) pada sebuah towing tank. Dari penelitian ini didapatkan rasio amplitudo, A/D rata – rata terbesar berada pada variasi d/D = 2 sebesar 0,154. Nilai A/D dan Cl relatif menurun seiring dengan meningkatnya center to center spacing.
EVALUASI PERFOMANSI PENAMBAHAN GUIDE VANE PADA PENAMPANG CIRCULAR MITER BEND 300 DENGAN STUDI NUMERIK ( STUDI KASUS REYNOLDS NUMBER ReDh 4,744 107 ) Achjab, Firmansyah; Nugroho, Setyo; l Ulum, Achmad Bahrul
Proceeding SENDI_U 2015: SEMINAR NASIONAL MULTI DISIPLIN DAN CALL FOR PAPERS
Publisher : Proceeding SENDI_U

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Abstract

Miter bending or curved pipe on the canal of penstock in hydropower has a pressure loss (pressure drop). Thishappens because of the curved shape of the miter bending that causes pressure on the outer wall (outter) biggerand blocking off the pace of the fluid. The value of the pressure loss is affected by the angle of the elbow as wellas a large radius of curvature, which causes secondary flow (secondary flow) resulting in large pressure loss sothat required an effort to minimize this pressure loss. Experiment model is miter bending 300 based on the finalbook drawing hydropower. Research numerically using ANSYS 14.5 with ReDh 4.744 reynolds number 107, themeasured variable is the static pressure and streamlined speed.
CFD Analysis of Nozzle Exit Position Effect in Ejector Gas Removal System in Geothermal Power Plant Nugroho, Setyo; Citrahardhani, Ciptananda
EMITTER International Journal of Engineering Technology Vol 3, No 1 (2015)
Publisher : Politeknik Elektronika Negeri Surabaya (PENS)

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Abstract

The single stage ejector is used to extract the Non CondensableGas (NCG) in the condenser using the working principle of the Venturi tube. Three dimensional computational simulation of the ejector according to the operating conditions was conducted to determine the flow in the ejector. Motive steam entering through the convergent – divergent nozzle with increasing flow velocity so that the low pressure exist around the nozzle. Comparison is done also in a two dimensional simulation to know the differences occurring phenomena and flow inside ejector. Different simulation results obtained between two dimensional and three dimensional simulation. Reverse flow which occurs in the mixing chamber made the static pressure in the area has increased dramatically. Then the variation performed on Exit Nozzle Position (NXP) to determine the changes of the flow of the NCG and the vacuum level of the ejector.Keywords: Ejector, NCG, CFD, Compressible flow.
CFD Analysis of Nozzle Exit Position Effect in Ejector Gas Removal System in Geothermal Power Plant Nugroho, Setyo; Citrahardhani, Ciptananda
EMITTER International Journal of Engineering Technology Vol 3, No 1 (2015)
Publisher : Politeknik Elektronika Negeri Surabaya (PENS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (471.329 KB) | DOI: 10.24003/emitter.v3i1.35

Abstract

The single stage ejector is used to extract the Non CondensableGas (NCG) in the condenser using the working principle of the Venturi tube. Three dimensional computational simulation of the ejector according to the operating conditions was conducted to determine the flow in the ejector. Motive steam entering through the convergent – divergent nozzle with increasing flow velocity so that the low pressure exist around the nozzle. Comparison is done also in a two dimensional simulation to know the differences occurring phenomena and flow inside ejector. Different simulation results obtained between two dimensional and three dimensional simulation. Reverse flow which occurs in the mixing chamber made the static pressure in the area has increased dramatically. Then the variation performed on Exit Nozzle Position (NXP) to determine the changes of the flow of the NCG and the vacuum level of the ejector.Keywords: Ejector, NCG, CFD, Compressible flow.
Performance Analysis of The Effect on Insertion Guide Vanes For Rectangular Elbow 900 Cross Section Nugroho, Setyo; Hidayatulloh, Achmad Arifudin
EMITTER International Journal of Engineering Technology Vol 4, No 2 (2016)
Publisher : Politeknik Elektronika Negeri Surabaya (PENS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1875.344 KB) | DOI: 10.24003/emitter.v4i2.157

Abstract

The use of elbow or curved pipe in the installation of piping has a loss of pressure (pressure drop) which could lead the power of pump that drive the fluid and decrease the energy efficiency of the system. The pressure drop is caused by the curved shape of the elbow that cause pressure on the outer wall (outter) larger and blocking off the pace of the fluid, and flow pressure losses caused by friction, flow separation and secondary flow. A method that can be used to reduce flow separation and pressure loss in the elbow is by the insertion guide vane. The test model in the form of rectangular elbow 900  with a radius ratio (rc/Dh) = 1.1249 without using a guide vane and number of guide vane insertion one until three guide vanes. With Reynolds number ReDh ≈ 8.6 × 104. The velocity inlet is uniform, the measured variable is static pressure. Static pressure was measured using an inclined manometer. With variation the number of guide vane gives a more effect on the value of pressure drop, the largest pressure drop until 123.35% compared to that without guide vane. The velocity distribution profile on the outlet side becomes more uniform. The magnitude of this pressure drop occurs as a result of the increased flow friction and its secondary flow become smaller.
Improve of Water Flow Acceleration in Darrieus Turbine Using Diffuser NACA 11414 2,5R Safitra, Arrad Ghani; Aribowo, Teguh Hady; Nugroho, Setyo; Julianto, Mochammad Arief
EMITTER International Journal of Engineering Technology Vol 6, No 1 (2018)
Publisher : Politeknik Elektronika Negeri Surabaya (PENS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24003/emitter.v6i1.236

Abstract

Indonesia has potential hydro energy around 70000 MW which has been used around 6% (3529 MW). One of the development constraint is the stream velocity in Indonesian rivers is relative low. It causes bigger turbine dimension needed to achieve power which is desired.  An alternative is to utilize adiffuser, which is a device that could accelerate the fluid flow in order to give more energy to the turbine. Based on contiunity equation, diffuser can increase velocity by ratio of cross-section area. It can be  used to achieve expected power as long as it is not too much reduce the pressure. This research is conducted in 0.566 m/s of water velocity with Darrieus turbine with hydrofoil NACA 0018, height 0.74 m, radius 0.17 m, chord 0.11 m and 3 number of blades. The performance (Cp) was determined by numerical and experimental without and with diffuser NACA 11414 2.5R for variation of angle 8o, 16o, and 20o. Both of those result showed that the best performance of NACA 11414 2,5R is on angle 16o which numerically has stream velocity 0,91 m/s of water and 7 times of Cp, while experimentally has 0,891 m/s of water velocity and 3,16 times of Cp. This diffuser could improve the power generated by the turbine and increase the turbine efficiency.
Efect of Gamma 60Co Irradiation on The Growth, Lipid Content and Fatty Acid Composition of Botryococcus sp. Microalgae Ermavitalini, Dini; Yuliansari, Niki; Prasetyo, Endry Nugroho; Saputro, Triono Bagus
Biosaintifika: Journal of Biology & Biology Education Vol 9, No 1 (2017): April 2017
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v9i1.6783

Abstract

Botryococcus sp. is one of microalgae species that has a high lipid content as much as 75% of their dry weight. But, lipid production by microalgae is regulated by their environmental condition (pH, light, temperature, nutrition, etc). Mutagenesis induced by Gamma 60Co irradiation can be utilized to alter the Botryococcus sp. genetic to get microalgae mutant strain that can produce a higher lipid content than the wild strain. Botryococcus sp. was irradiated with different doses of gamma ray of 60Co (0, 2, 4, 6, and 10 Gy), and the effect on the growth, lipid content, and fatty acid composition of microalgae were observed. Research design used is random complete (RAL) with 95 % confident level for quantitive analysis based on the biomass and lipid contents. More over fatty acid composition was analyzed by Gas Cromatography-Mass Spectrometry (GC-MS). Results showed that Gamma irradiated gave an effect on growth and lipid content of Botryococcus sp. But between the control treatment (0 Gy) with microalgae irradiated dose of 2 Gy, 4 Gy and 6 Gy were not significantly different. Whereas between the control with 10 Gy irradiated was significantly different. The highest biomassa and lipid content are found in 10 Gy irradiated microalgae with 0.833 gram biomass and 41% lipid content. Fatty acid profile of Botryococcus sp. control has 6 fatty acids while 10 Gy irradiated microalgae has 12 fatty acids, with the long-chain fatty acids increased, whereas short-chain fatty acids decreased.
Pengaruh Teknik Sterilisasi dan Komposisi Medium terhadap Pertumbuhan Tunas Eksplan Sirsak Ratu Sulistiyo, Rico Hutama; Luthfiyyah, Zayyan; Susilo, Buana; Dalimartha, Lengga Nurullah; Wiguna, Eko Chandra; Yuliana, Nuniek; Prasetyo, Endry Nugroho
Bioedukasi: Jurnal Pendidikan Biologi Vol 11, No 1 (2018): BIOEDUKASI: Jurnal Pendidikan Biologi
Publisher : Department of Biology Education Faculty of Teacher Training and Education Sebelas Maret Un

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (312.619 KB) | DOI: 10.20961/bioedukasi-uns.v11i1.19726

Abstract

Successful tissue culture of soursop plants requires proper sterilization techniques and hormone levels to produce good shoot growth. This study aims to determine the effect of sterilization techniques and medium composition of soursop explants, using factorial completely randomized design with 2 factors: sterilizing agents and medium composition, repeated 3 times. Hydrogen Peroxide, Mercury and Sodium Hypoclorite used as sterilizing agents. The medium composition used in this study consisted of 3 levels: MS 0, MS 0.5 ppm BAP + 0.05 ppm NAA and MS 1 ppm BAP + 0.1 ppm NAA. The best treatment is MS medium 0.5 ppm BAP + 0.05 ppm NAA with hydrogen peroxide sterilizing agent which produces the highest propagation level and lowest contamination level.