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All Journal Jurnal Teknologi Dan Industri Pangan Jurnal Teknologi Industri Pertanian Microbiology Indonesia BIOTROPIA - The Southeast Asian Journal of Tropical Biology Indonesian Journal of Agricultural Science Jurnal AgroBiogen Jurnal Penelitian Pascapanen Pertanian Pengembangan Inovasi Pertanian Buletin Teknologi Pasca Panen agriTECH PROSIDING SEMINAR KIMIA BERITA BIOLOGI Informatika Pertanian Indonesian Journal of Chemistry Menara Perkebunan Pharmaceutical Sciences and Research (PSR)
Nur Richana
Universitas Ibn Khaldun
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Engineering Immunology & microbiology Industrial & Manufacturing Engineering Medicine & Pharmacology Veterinary

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POTENTIAL USE OF AN EXTRACELLULAR ENZYME OF a-AMYLASE FROM INDIGENOUS INDONESIAN MESOPHILIC BACTERIA Lestari, Puji; Richana, Nur; Masriani, Rina
Indonesian Journal of Agricultural Science Vol 14, No 1 (2013): April 2013
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Amylase enzyme has a great significance for industrial usages in  Indonesia. However, this enzyme is still imported. The use of bacteria in biotechnological process of industrial products such as enzyme production has stimulated the exploration of extracellular amylase producing  bacteria. This study aimed to identify and analyze the potential use of amylolytic bacterial enzymes for hydrolyzing cassava starch. Two bacterial isolates, i.e. MII-10 and DKW-8 originated from Indonesia soil were identified based on their morphological, physiological and biochemical properties according to the standard protocol. The isolates were then  cultivated on fermentation medium and their growth pattern and  enzymatic assays were observed. The acetone-precipitated crude enzyme harvested based on predetermined cultivation time was used for  enzymatic hydrolysis product characterization on cassava starch using thin layer chromatography (TLC). The results showed that the mesophilicbacteria isolates (MII-10 and DKW-8) were belonged to Bacillus licheniformis. The maximum bacterial cell growth and enzyme activity were reached at 48 hours after incubation. The MII-10 isolate was found more stable than DKW-8 in producing amylase enzyme. Amylase produced by the MII-10 and DKW- 8 isolates was identified to be an endo-a-amylase as confirmed by oligosaccharides and dextrin of the random hydrolysisproducts. Relatively high dextrose equivalence (DE) value of a-amylase of MII-10 (DE of 9.96) suggests that the enzyme is prospective for  saccharification of starchy material in glucose syrup industry.
KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus acidilactici F-11 [Characterization of the Pediocin PaF-11 Encoding Gene in Pediococcus acidilactici F-11] Marwati, Tri; Richana, Nur; Harmayani, Eni; Rahayu, Endang S
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.137 KB) | DOI: 10.14203/beritabiologi.v11i2.495

Abstract

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker λDNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101.
KARAKTERISASI DAN STUDI STABILISASI a-AMILASE Bacillus licheniformis TVII.6 MENGGUNAKAN BAHAN ADITIF Lestari, Puji; Richana, Nur; Rosmimik, Rosmimik
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (400.712 KB) | DOI: 10.14203/beritabiologi.v10i5.1914

Abstract

The limited stability of enzyme during long-term storage attributes to its reduced function. In this study, a-amylase from Bacillus licheniformis TVII.6 were formulated with different kind of additives for storage stabilization and better performance. Simultaniously, some minerals and calcium ion were applied to elucidate the inhibition and activation effects to a-amylase. Crude enzyme which was prepared by aceton precipitation was used for this stability test. It demosntrated that 10% of mannitol in citrate phosphate buffer gave the highest residual activity after 3 months of storage (98.5%). Calcium ion influenced the thermal stability of a-amylase and it gave optimum activity at 5 mM CaCl , thus the stability increased from 76.0%/90 C/2 hours to 114.8%/90 C/22 hours in comparison without calcium ions. Calcium ions (5 mM CaCl ) on the stability of a-amylase at 4 C also produced the 2highest residual activity, which remained 100% during 48 hours of incubation. Chemical compounds like FeSO , Na CO and EDTA 4 2 3acted as inhibitors, while (NH ) SO , CuSO , CoSO , MgCl and K HPO did not inhibit activity of a-amylase. NaOH and MnCl 4 2 4 4 4 2 2 4 2 compounds at low concentrations (2 mM) did not inhibit the enzyme but at 10 mM became a-amylase inhibitors. This amylase stability information is very important as a consideration when applying and storing the enzyme, thereby reducing the degradation a-amylase activity.
TEKNOLOGI BIOPROSES JAGUNG DAN UBI KAYU MENDUKUNG KETAHANAN PANGAN DAN ENERGI Richana, Nur
Pengembangan Inovasi Pertanian Vol 7, No 1 (2014): Maret 2014
Publisher : +622518321746

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/pip.v7n1.2014.31-40

Abstract

Tingkat konsumsi beras yang tinggi dan melonjaknya impor terigu dan gula merupakan masalah utama dalam memenuhi kebutuhan pangan di Indonesia. Teknologi bioproses dengancara enzimatis maupun mikrobiologis untuk beras nonpadi ataupun tepung-tepungan dari bahan lokal mampu meningkatkan mutu produk sehingga dapat bersaing dengan beras dan terigu. Demikian juga gula cair dapat dibuat dengan cara enzimatis dan mempunyai prospek yang menjanjikan untuk mengurangi impor gula. Pengembangan teknologi bioproses dapat meningkatkan cita rasa, citra, dan daya saing produk pangan dari jagung dan ubikayu sebagai pengganti beras, terigu, dan gula tebu. Untuk mengurangi kompetisi pemanfaatan produk pertanian untuk pangan dan energi, pencarian sumber energi alternatif menjadi sangat penting. Limbah hasil pertanian merupakan sumber bahan bakar yang menjanjikan. Dengan teknologi bioproses, limbah jagung dan ubi kayu dapat diolah menjadi bioetanol sebagai bahan bakar nabati. Pengadaan energi dari limbah pertanian tidak mengganggu pengadaan pangan sehingga mendukung ketahanan pangan.
MENELISIK TITIK KRITIS PENGEMBANGAN TEKNOLOGI PENGOLAHAN HASIL PERTANIAN Munarso, S. Joni; Richana, Nur
Pengembangan Inovasi Pertanian Vol 7, No 1 (2014): Maret 2014
Publisher : +622518321746

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/pip.v7n1.2014.41-49

Abstract

Agroindustri memberikan kontribusi positif dan mendominasi pangsa terhadap Produk Domestik Bruto (PDB). Namun, tren kontribusi ini menurun dari 27,83% pada 2006 menjadi 25,49% pada 2011. Kelesuan agroindustri ini perlu segera diatasi untuk mencapai target pembangunan pertanian melalui peningkatan nilai tambah. Selaras dengan itu, peran teknologi pengolahan hasil pertanian sebagai komponen penting dalam pengembangan agroindustri perlu dikaji, mengingat teknologi yang tersedia belum digunakan secara maksimal oleh pengguna. Pengembangan teknologi pengolahan hasil pertanian memiliki empat titik kritis, yaitu tahap penyusunan komponen teknologi, perakitan paket teknologi, pengembangan model agroindustri, dan implementasi model agroindustri. Pada setiap titik kritis ini terdapat masalah yang berpotensi menghambat penerapan teknologi. Minimnya keterlibatan calon pengguna dalam perancangan penelitian memunculkan kebingungan dalam mengarahkan hasil perakitan teknologi. Filosofi quick yielding research sering mendorong terjadinya penghilangan tahap perakitan paket teknologi, yang berakibat munculnya masalah teknis dalam pengembangan moral maupun implementasi model agroindustri. Tahap perakitan paket teknologi menjadi titik terlemah saat ini. Penguatan infrastruktur dan program riset serta penguatan modal sosial (social capital) pada tahap pengembangan model agroindustri merupakan kebijakan strategis untuk mendukung pengembangan teknologi menuju kemantapan penerapannya dalam pembangunan agroindustri.
PENGGANDAAN SKALA PRODUKSI BIOETANOL DARI TONGKOL JAGUNG Arif, Abdullah bin; Diyono, Wahyu; Hayuningtyas, Maulida; Syaefullah, Enrico; Budiyanto, Agus; Richana, Nur
Informatika Pertanian Vol 26, No 2 (2017): Jurnal Informatika Pertanian
Publisher : Sekretariat Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1043.859 KB) | DOI: 10.21082/ip.v26n2.2017.p57-66

Abstract

The effort to search for alternative energy materials that do not compete with food and feed is necessary and urgent. Lignocellulosic biomass is one potential source of renewable energy. Scalinge up methodproduction of bioenergy production from laboratory scale to industrial scale needs to be studied and developed. The aim of this study is to find get scalinge up method o0f the bioethanol  production from corn cobs. An Eexperiments on scalinge up of bioethanol production from laboratory scale to industrial scale was is done by the Pg / V constant method (stirring power per volume). Scale up calculations based on data from fermented liquid rheological characteristics and specifications fermenters are used. The results showed that the calculation of basic scale up bioethanol production capacity bioreactor of 200 l, obtained working volume of 65% or 130 l, high of liquid fermentation  0.840 m, diameter tank bioreactor 0.441 m, diameter of a stirrer of turbine type of flat 0.187 m and the speed of agitation at 66.34 rpm. Based on  the calculation of basic scale up bioethanol production capacity bioreactor of 10,000 l, obtained working volume of 65% amounting to 6,500 l, high of liquid fermentation  2.87 m, diameter tank bioreactor 1.49 m, diameter of a stirrer of turbine type of flat 0.63 m and the speed of agitation at 29.52 rpm.
Purifikasi dan Karakterisasi α-amilase Termostabil dari Bacillus stearothermophilus TII-12 Lestari, Puji; Richana, Nur; Darwis, Abdul Aziz; Syamsu, Khaswar; Murdiyatmo, Untung
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p56-62

Abstract

Purification and Characterization of Thermostableα-amylase from Bacillus stearothermophilus TII-12. PujiLestari, Nur Richana, Abdul A. Darwis, Khaswar Syamsu,and Untung Murdiyatmo. Thermostable α-amylase is apotential enzyme employed in the starch processing andwidely used in food industries, but this enzyme is stillimported. The local enzyme production would be moreeconomist and useful for its broad applications. Here wereport α-amylase from indigenous bacteria TII-12 which waspurified and characterized, as well as analyzed its hydrolysisproduct on cassava starch. The enzyme of Bacillusstearothermophilus TII-12 partially purified by ultrafiltration,acetone precipitation and gel filtration (Sephadex G-100)showed the reduced total activity, total protein and yield, butincreased the specific activity. The enzyme had a Km of 1,06mg/ml and Vmax of 1,21 mol/min, with optimal activity at pH 7and 90oC. An apparent molecular mass was of 192.932,8Dalton, as estimated by Native-Polyacrylamide Agarose Gelelectrophoresis. Its activity was inhibited by the divalentcation chelator such as EDTA and CuSO4 but activated bycalcium ion. Hydrolysis products of this enzyme on cassavastarch were glucose, dextrin, maltose and oligosaccharides.After 24 hours of hydrolysis, the concentration of glucoseand maltose reached 51.970 and 10.090 ppm, respectively.The thermostable α-amylase of TII-12 is an endo-α-amylaseand prospective to be applied on starch liquefaction withhigh temperature process.
ANALISIS RANCANGAN FAKTORIAL TIGA FAKTOR UNTUK OPTIMALISASI PRODUKSI BIOETANOL DARI MOLASES TEBU Arif SP.MSi, Abdullah Bin; Diyono, Wahyu; Budiyanti, Agus; Richana, Nur
Informatika Pertanian Vol 25, No 1 (2016): JUNI 2016
Publisher : Sekretariat Badan Penelitian dan Pengembangan Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (521.958 KB) | DOI: 10.21082/ip.v25n1.2016.p145-154

Abstract

Bioethanol is a plant-based fuels potential as alternative materials to substitute fossil fuels which are non-renewable. The aim of this study is to get the optimal dose of Saccharomyces cerevisiae as starer, concentration of nitrogen (urea) and fermentation time for the production of bioethanol effectively and efficiently. This study was conducted at the Laboratory of Postharvest Research and Development Institute, Bogor from May to September 2015. The materials used are molasses of sugar cane. The experimental design used was a factorial design with 3 factors. The first factor was the treatment of fermentation time (1, 2 and 3 days). The second factor was the nitrogen concentration (0, 2 and 4 grams). The thirtd factor was the Saccharomyces cerevisiae concentration (1, 1.5 and 2 grams). Each treatment was repeated twice. Observations were made on the characteristics of the raw materials (total sugar content, ash content and calcium content) and the resulting product (total sugar content, alcohol content and yield). Data were analyzed using analysis of variance and Duncan Multiple Range Test (DMRT). The results showed that the addition of starter Saccharomyces cerevisiae and treatment of urea 2 grams each produced the highest yield alcohol with a fermentation time of 3 days.
ISOLATION AND IDENTIFICATION OF MICROORGANISMS DURING SPONTANEOUS FERMENTATION OF MAIZE [Isolasi dan Identifikasi Mikroorganisme pada Fermentasi Spontan Jagung] . Rahmawati; Ratih Dewanti -Hariyadi; Purwiyatno Hariyadi; Dedi Fardiaz; Nur Richana
Jurnal Teknologi dan Industri Pangan Vol. 24 No. 1 (2013): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (575.448 KB) | DOI: 10.6066/jtip.2013.24.1.33

Abstract

Maize was traditionally the second most common staple food in Indonesia. Conversion to maize flour has been accomplished to improve its convenience. Traditionally, maize flour is produced by soaking the kernels in water followed by grinding. It was reported that final physicochemical characteristics of the maize flour were influenced by spontaneous fermentation which occurred during soaking. This research aimed to isolate and identify important microorganisms that grew during fermentation thus a standardized starter culture can be developed for a more controlled fermentation process. Soaking of maize grits was conducted in sterile water (grits:water=1:2, w/v) in a closed container at room temperature (±28ºC) for 72 hours. After 0, 4, 12, 24, 36, 48, 72 hours, water and maize grits were sampled and tested for the presence of mold, yeast, and lactic acid bacteria (LAB). Isolates obtained from the spontaneous fermentation were reinoculated into the appropriate media containing starch to observe their amylolytic activity. Individual isolate was then identified; mold by slide culture method, while yeast and LAB by biochemical rapid kits, i.e. API 20C AUX and API CH50, respectively. The number of each microorganism was plotted against time to obtain the growth curve of the microorganisms during spontaneous fermentation. The microorganisms were identified as Penicillium chrysogenum, P. citrinum, A. flavus, A. niger, Rhizopus stolonifer, R.oryzae, Fusarium oxysporum, Acremonium strictum, Candida famata, Kodamaea ohmeri, Candida krusei/incospicua, Lactobacillus plantarum 1a, Pediococcus pentosaceus, L. brevis 1, L. plantarum 1b, and L. paracasei ssp paracasei 3. Four molds and one yeast were amylolytic while none of the LAB was capable of starch hydrolysis. The growth curve suggested that the amylolitic mold and yeast grew to hydrolyze starch during the course of fermentation, while the LABs benefited from the hydrolyzed products and dominated the later stage of the fermentation.
PENGARUH LAMA HIDROLISIS ASAM TERHADAP KARAKTERISTIK FISIKO-KIMIA PATI GARUT Christina Winarti, Nur Richana, Djumali Mangunwidjaja, Titi Candra Sunarti TIP
Jurnal Teknologi Industri Pertanian Vol. 24 No. 3 (2014): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRAK Hidrolisis asam secara lambat (lintnerisasi) merupakan salah satu proses modifikasi pati untuk menghasilkan pati viskositas rendah. Hidrolisis asam dapat dilakukan sebagai perlakuan awal pada proses modifikasi lainnya. Tujuan penelitian adalah mengetahui pengaruh hidrolisis asam secara lambat terhadap karakteristik pati termodifkasi terutama morfologi permukaan dan sifat fungsionalnya sebagai bahan matriks. Perlakuan yang dicobakan lama hidrolisis yaitu 2, 4, 6, 24, 72, dan 120 jam menggunakan HCl 2,2 N pada suhu 35°C. Parameter yang diamati meliputi Derajat Polimerisasi (DP), tingkat hidrolisis, kadar amilosa, morfologi, kristalinitas, kelarutan dan swelling power, kemampuan menyerap air (water absorption capacity/WAC) dan minyak (oil absorption capacity/OAC) dan daya cerna pati. Hasil penelitian menunjukkan bahwa rendemen dan DP semakin menurun sementara tingkat hidrolisis meningkat dengan semakin lamanya lintnerisasi, kadar amilosa menurun sementara daya cernanya mula-mula menurun kemudian meningkat. Granula pati mengalami kerusakan walaupun bentuknya masih relatif tidak berubah demikian juga nilai kristalinitas relatif meningkat walaupun pola kristal tetap tipe A, dan tidak memberikan sifat pasting selama gelatinisasi. Kelarutan dan WAC serta OAC meningkat sedangkan swelling power (SP) menurun, sementara OAC lebih tinggi dari WAC. Kata kunci: Maranta arundinacea, pati garut, lintnerisasi, hidrolisis asam  
Co-Authors A.A. Ketut Agung Cahyawan W Abdullah bin Arif Abdullah Bin Arif Achmad Zainudin Agus Budiyanto Agus Budiyanto ANJA MERYANDINI Anwar Nur Anwar Nur Arif SP.MSi, Abdullah Bin Arif, Abdullah bin Budiyanti, Agus Budiyanto, Agus Christina Winarti Darwis, Abdul Aziz Dedi Fardiaz Diyono, Wahyu Diyono, Wahyu Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi Setyaningsih EKA RURIANI Eka Ruriani Endang Prangdimurti Endang S Rahayu Endang S Rahayu Eni Harmayani Eni Harmayani Enrico Syaefullah Erliza Noor Evi Savitri Iriani Faizal Gayang Firda DIMAWARNITA Hanny Hafiar Hayuningtyas, Maulida I Ketut Suada I Wayan Arnata Illah Sailah Irinne D.P Khaswar Syamsu Lestari, Puji M. ANWAR NUR M. EDY SOFIYANTO Maria Bintang Maulida Hayuningtyas Munarso, S. Joni Murdiyatmo, Untung nFN Suliantari nFN Widaningrum Puji Lestari PUJI LESTARI Puji Lestari Purwiyatno Hariyadi Rahmawati Rahmawati Ratih Dewanti -Hariyadi Ratnaningsih Ratnaningsih Rina Masriani Rosmimik Rosmimik Rosmimik, Rosmimik Sakae Suzuki Sri Laksmi Suryaatmadja Sri Usmiati Titi C Sunarti Titi C. Sunarti Titi Candra Sunarti dan Michael (E-Jurnal Agro-Industri Indonesia) Titi Chandra Sunarti Tri Marwati Tri Marwati Tri Marwati Tri Panji Tun T. Irawadi Tun Tedja Irawadi Wahyu Diyono Wahyu Diyono Yandra Arkenan Yoshiharu Fujii