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ISOLASI DAN KARAKTERISASI BAKTERI PENGHASIL ENZIM LIPASE EKSTRASELULERDARI LUMPUR AKTIF INSTALASI PENGOLAHAN AIR LIMBAH INDUSTRI TEKSTIL Kasipah, Cica; Rismayani, Sinta; Ihsanawati, Ihsanawati; Nurachman, Zeily
Arena Tekstil Vol 28, No 1 (2013)
Publisher : Arena Tekstil

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3387.131 KB)

Abstract

Lumpur aktif dari instalasi pengolahan air limbah industri tekstil mengandung berbagai jenis mikroorganisme antara lain mikroorganisme yang memiliki aktivitas lipase yang tinggi. Tujuan penelitian ini adalah mengisolasi dan mengkarakterisasi bakteri penghasil enzim lipase ekstraseluler dari lumpur aktif instalasi pengolahan air limbah industri tekstil. Tahapan penelitian yang dilakukan meliputi isolasi bakteri penghasil enzim lipase ekstraseluler dari lumpur aktif melalui skrining dengan media yang mengandung rodamin dan minyak zaitun, penentuan aktivitas enzim lipase dan karakterisasi lipase yang dihasilkan terhadap variasi temperatur, pH, dan pengaruh ion Ca2+. Hasil identifikasi secara mikrobiologi menunjukkan bahwa bakteri penghasil enzim lipase ekstraseluler dari lumpur aktif adalah spesies Erwiniachrysantemi. Enzim lipase ekstraseluler tersebut memiliki aktivitas 4,75 U/mL dengan temperatur optimum 40oC dan pH optimum 9. Penambahan ion Ca2+ tidak memberikan pengaruh berarti terhadap aktivitas enzim lipase.
Isolation and Characterization of Phytase from Chicken Manure Bacteria Irawan, Mega Pratiwi; Nurachman, Zeily
Jurnal Natur Indonesia Vol 15, No 2 (2013)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.527 KB) | DOI: 10.31258/jnat.15.2.99-105

Abstract

Cereals in animal feed contain anti-nutrients of phytic acid that has capability of chelating proteins and cations. Phytasecan be employed to reduce phytic acid through hydrolyzing phytic acid into free phosphate group and lower derivate ofinositol phosphate. The aim of the study was to isolate and characterize phytase obtained from chicken manure bacteria.The study included the screening of phytase-producing bacteria from chicken manure, the homology analysis of bacterium,and the determination of phytase activity. Phytase activity was measured from concentration of free phosphate. The resultsshowed that one phytase-producing isolate obtained from chicken manure grew in the medium containing 5% rice branextract at 37°C for 5 d. Based on a phylogeny is tree analysis of the genes related to 16S rRNA, the isolate was identified asAcinetobacter sp. TZ1. The extracellular phytase expressed by Acinetobacter sp. TZ1 exhibited optimum reactions at pH5 and 50°C. The enzyme showed activity of 64,6 nmol mL–1min–1 and specific activity of 236 nmol min–1mg–1. Relativemolecular mass of phytase TZ1 was ~35 kDa. Phytase obtained is potential to improve animal feed quality by hydrolyzingphytic acid.
PEMBELAJARAN MAKROMOLEKUL: PEMBUATAN HIDROLISAT PROTEIN Sinaga, Kelly; Nurachman, Zeily
Proceeding Biology Education Conference: Biology, Science, Enviromental, and Learning Vol 10, No 1 (2013): Seminar Nasional X Pendidikan Biologi
Publisher : Universitas Sebelas Maret

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Abstract

Penelitian ini bertujuan untuk membuat bahan pembelajaran fungsi enzim  dalam metabolisme  dan materi makromolekul  di sekolah menengah melalui eksperimen pembuatan hidrolisat protein  dengan papain. Diharapkan penelitian ini mampu memberi manfaat dalam membantu guru memberikan contoh nyata aplikasi peranan enzim dan materi makromolekul dalam kehidupan sehari-hari. Enzim yang digunakan dalam penelitian ini adalah protease. Protease dari pepaya dikenal sebagai papain. Hidrolisis protein dengan papain kasar menghasilkan hidrolisat protein. Pada penelitian ini, aktivitas papain diuji terhadap substrat kasein  dan penentuan konsentrasi protein hasil hidrolisis dilakukan dengan metode Lowry. Hasil yang diperoleh adalah nilai aktivitas spesifik papain sebesar 7,93 U/mg,. Dari hasil kinetika reaksi enzim diperoleh nilai konstanta Michaelis-Menten (KM) papain 0,028% dengan Vmaks  = 0.75 gram/menit. Kegiatan meneliti dengan topik pembuatan hidrolisat protein ini dapat dipakai sebagai bahan penyusunan model pembelajaran materi peranan enzim dan makromolekul di Sekolah Menengah Atas. Pengalaman meneliti seperti mengisolasi enzim kasar dari bahan alam dan menguji kinetika reaksi enzim merupakan praktik yang dapat diajarkan kepada siswa sehingga mereka dapat memahami pelajaran biologi mengenai peranan enzim dan makromolekul lebih baik. Penelitian  ini juga dapat membantu atau menguatkan pemahaman materi ajar lain seperti laju reaksi, ikatan kimia, dan reaksi kimia organik sehingga tercipta pembelajaran biologi yang terpadu dengan pembelajaran lainnya. Kata kunci : enzim, protease, papain, protein, pembelajaran terpadu
Isolasi Karakterisasi Bakteri Pendegradasi Asam Monokloroasetat dari Tanah Vina Juliana Anggraeni; Enny Ratnaningsih; Zeily Nurachman
EduChemia (Jurnal Kimia dan Pendidikan) Vol 2, No 2 (2017): Available Online in July 2017
Publisher : Department of Chemical Education Faculty of Teacher Training and Education Universitas Su

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (443.593 KB) | DOI: 10.30870/educhemia.v2i2.1327

Abstract

Senyawa organohalogen merupakan salah satu polutan terbesar di lingkungan. Bioremediasi merupakan salah langkah yang dapat dilakukanuntuk mengurangi polusi organohalogen. Beberapa bakteri tanah diketahui memiliki enzim dehalogenase dan berpotensi digunakan sebagai bioremediator senyawa organohalogen. Pada penelitian ini, dilakukan isolasi terhadap bakteri tanah yang mampu mendegradasi Asam Monokloroasetat (MCA) dan mengkarakaterisasi kemampuan tumbuh bakteri-bakteri tersebut pada berbagai konsentrasi MCA. Hasil isolasi didapatkan5 koloni bakteri yang mampu tumbuh pada medium dengankonsentrasi MCA yang tinggi yaitu 10mM. BakteriPG3, TJ4, PW2,CW1, dan PG2  masing-masing mampu melepaskanion klorida sebesar 95,14%; 91,89%; 89,46%; 89,46; 88,81%  pada medium yang mengandung1mM MCA 29,24%; 28,17%; 28,10%; 24,31%; 26,16%  pada 5mM MCA,  dan  13,03%; 12,09%; 9.95%; 8,35%; 8,72% pada 10mM MCA. Terlihat bahwa degradasi organohalogen  terjadi  lebih efektif pada medium dengan konsentrasi MCA rendah, dan bakteri PG3 mempunyai kemampuan yang tertinggi.Pertumbuhan kelima bakteri mencapai fasa stationer pada18-24 jam denganOD600 sebesar 0.3-0.4. Senyawa organohalogen merupakan salah satu polutan terbesar di lingkungan. Bioremediasi merupakan salah langkah yang dapat dilakukanuntuk mengurangi polusi organohalogen. Beberapa bakteri tanah diketahui memiliki enzim dehalogenase dan berpotensi digunakan sebagai bioremediator senyawa organohalogen. Pada penelitian ini, dilakukan isolasi terhadap bakteri tanah yang mampu mendegradasi Asam Monokloroasetat (MCA) dan mengkarakaterisasi kemampuan tumbuh bakteri-bakteri tersebut pada berbagai konsentrasi MCA. Hasil isolasi didapatkan5 koloni bakteri yang mampu tumbuh pada medium dengankonsentrasi MCA yang tinggi yaitu 10mM. BakteriPG3, TJ4, PW2,CW1, dan PG2  masing-masing mampu melepaskanion klorida sebesar 95,14%; 91,89%; 89,46%; 89,46; 88,81%  pada medium yang mengandung1mM MCA 29,24%; 28,17%; 28,10%; 24,31%; 26,16%  pada 5mM MCA,  dan  13,03%; 12,09%; 9.95%; 8,35%; 8,72% pada 10mM MCA. Terlihat bahwa degradasi organohalogen  terjadi  lebih efektif pada medium dengan konsentrasi MCA rendah, dan bakteri PG3 mempunyai kemampuan yang tertinggi.Pertumbuhan kelima bakteri mencapai fasa stationer pada18-24 jam denganOD600 sebesar 0.3-0.4. 
Domain Motion in 3-isopropylmalate dehydrogenase: A Strategy to Enhance its Thermal Stability Zeily Nurachman; Tairo Oshima; Nobuo Tanaka
Journal of Mathematical and Fundamental Sciences Vol. 35 No. 2 (2003)
Publisher : Institute for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/itbj.sci.2003.35.2.6

Abstract

In order to elucidate the thermal properties of Thermus thermophilus 3-isopropylmalate dehydrogenase, mutant structures with mutations at the C-terminus were compared with each other. The structural movement can be anticipated from the structural changes among mutants in regions of a minor groove and pillar. Our previous studies revealed that the open-close movement of the active site groove antagonizes to that of the minor groove (like a paperclip) and the thermostability of the enzyme increases when the active site groove is closed. In the present study, it is shown that the motion of the enzyme mainly occurs in the first domain and strand D in the pillar structure is a hinge-bending region of the movement. The motion of the first domain to expand the minor groove may close the active site groove suggesting a mechanism for the enhanced thermal stability of 3-isopropylmalate dehydrogenase.
MODIFIKASISERAT KERTAS BEKAS MENGGUNAKAN ENDOGLUKANASE EgIII Rina Masriani; Zeily Nurachman
JURNAL SELULOSA Vol 2, No 02 (2012): JURNAL SELULOSA
Publisher : Center for Pulp and Paper

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (485.034 KB) | DOI: 10.25269/jsel.v2i02.33

Abstract

To reduce the water holding capacity by removing amorphous structure of cellulose in waste paper fibers, the use of endoglucanase EgIII was investigated. The steps of research included production of endoglucanase EgIII, modification of waste paper fibers using endoglucanase, and lab-scale making of sheet of paper. Compared to the freeness number of pulp without modification, the freeness number of waste paper fiber suspension treated using endoglucanase EgIII increased by 37 mL CSF (Canadian Standard Freeness). The burst index of paper sheet produced by endoglucanase EgIII treatment showed no significant change. Tensile index of paper sheet produced by endoglucanase EgIII increased by 5 Nm/g.Keywords: endoglucanase EgIII, freeness, burst index, tensile index ABSTRAK Untuk mengurangi kemampuan serat kertas menyerap air, endoglukanase EgIII diaplikasikan untuk memodifikasi serat kertas bekas. Modifikasi serat kertas bekas dilakukan untuk mengatasi masalah laju penghilangan air yang rendah pada serat kertas bekas. Tahapan penelitian yang dilakukan adalah produksi endoglukanase EgIII; modifikasi serat kertas bekas dengan cara menginkubasi campuran endoglukanase EgIII dan bubur kertas bekas hasil penggilingan; dan pembuatan lembaran kertas. Hasil penelitian menunjukkan bahwa nilai freeness bubur serat kertas bekas termodifikasi oleh endoglukanase EgIII meningkat 37 mL CSF (Canadian Standard Freeness) dibandingkan dengan nilai freeness bubur kertas tanpa modifikasi. Indeks retak lembaran kertas termodifikasi oleh endoglukanase EgIII tidak berubah secara signifikan. Selain itu, indeks tarik lembaran kertas termodifikasi oleh endoglukanase EgIII meningkat 5 Nm/g.Kata kunci: endoglukanase EgIII, freeness, indeks retak, indeks tarik 
Identification of Tropical Marine Diatom Chaetoceros dayaensis CBO from Bokor Island through Morphology and Genetic Marker Analysis Elva Stiawan; Sutomo Sutomo; Sherly Sapulete; Zeily Nurachman; Yanti Rachmayanti
Indonesian Journal of Chemical Studies Vol. 1 No. 1 (2022): Indones. J. Chem. Stud., June 2022
Publisher : Indonesian Scholar Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (671.509 KB) | DOI: 10.55749/ijcs.v1i1.2

Abstract

Microalgae are well-recognized by many researchers working on bioenergy resources due to its capability to produce high amounts of lipids as biodiesel precursors. Diatoms as a group of microalgae that specifically categorized by their silica valve could be more attractive to the researchers because in addition to high-lipid content, their silica features can be also utilized for wider applications, or used for diatoms bioproduct purification. Identification of some diatom species, such as the diatom genus Chaetoceros, as an initial step in diatom studies were difficult to accurately perform because of the unique morphology of silica frustules. Meanwhile, some candidate universal genetic markers of microalgae could also be used for the identification of diatoms with brittle frustules. In this research, we aimed to identify isolate CBO of tropical marine Chaetoceros CBO strain using morphological and genetic approaches. The isolate CBO of Chaetoceros sp. CBO was successfully obtained from sea region of Bokor Island, Kepulauan Seribu, Jakarta, and cultivated in our laboratory as culture stock for further use. Better resolution of the Chaetoceros CBO identification until species level was shown by genetic analysis toward two proposed gene markers for microalgae (rbcL-3P and V4 region of 18S rDNA) rather than by morphological. Specific DNA region for this specimen was found in V4 region of 18S rDNA genetic marker that could be recognized by MspA1I restriction enzyme. Hence, the RFLP (restriction fragment length polymorphism) method could be also used as an initial diagnostic tool for identification of this tropical marine Chaetoceros dayaensis CBO and for stock culture labelling purposes.
Immobilization of Crude Polyphenol Oxidase Extracts from Apples on Polypyrrole as a Membrane for Phenol Removal Anceu Murniati; Buchari Buchari; Suryo Gandasasmita; Zeily Nurachman; Arie Hardian; Dera Triani
Jurnal Kimia Sains dan Aplikasi Vol 24, No 2 (2021): Volume 24 Issue 2 Year 2021
Publisher : Chemistry Department, Faculty of Sciences and Mathematics, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3911.641 KB) | DOI: 10.14710/jksa.24.2.62-69

Abstract

This research aims to make a polypyrrole (PPy) membrane and crude extract of polyphenol oxidase (PPO) as a membrane of mPPy/PPO apple extracts. The membrane of PPy/PPO-apple extract has been synthesized by the electrodeposition method. The electrolyte composition consists of a mixture of 0.10-0.20 M pyrrole (Py) and 50-100% PPO apple extract, which is stable using 50 mM of phosphate buffer solution at pH 6.80-7.00 and room temperature. The electrodeposition process is used 400 mesh steel gauze anode ST-304 and carbon plate cathode. Electrodeposition is carried out at potential = 5.00-6.00 V; current = 0.02-0.25 A; the distance from both electrodes = 1.00-2.00 cm for 300-500 seconds. The results from the deposition of PPy/PPO apple extract of the anode are a membrane of mPPy/PPO-apple extract, with total enzyme activity (U) = (957,1441, 2287 and 1754) using 2.00-5.00 mM phenol as a substrate which is measured based on the UV-visible spectrophotometric method. PPy and mPPy/PPO-apple extracts were characterized by SEM and SEM-EDS. The membrane of mPPy/PPO-apple extract can be used to remove phenol in industrial wastewater samples is 50-65% with a filtration capacity of 500 mL for 2 hours.