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Siti Rosidah
Universitas Mataram
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Other

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PELATIHAN TEKNIK DASAR KULTUR JARINGAN TUMBUHAN Rina Kurnianingsih; Mursal Ghazali; Siti Rosidah; Aida Muspiah; Sri Puji Astuti; Aluh Nikmatullah
JMM (Jurnal Masyarakat Mandiri) Vol 4, No 5 (2020): November
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (455.659 KB) | DOI: 10.31764/jmm.v4i5.3049

Abstract

Abstrak: Kegiatan ini bertujuan untuk meningkatkan pengetahuan dan keterampilan mitra pengabdian tentang teknik kultur jaringan tanaman. Kegiatan pelatihan dibagi menjadi 2 sesi yaitu sesi pertama, penyampaian materi tentang konsep dasar kultur jaringan, kebutuhan peralatan dan bahan, media, zat pengatur tumbuh, kondisi lingkungan tumbuh, tahapan kultur jaringan dan macam-macam teknik kultur in vitro. Sesi kedua, praktek teknik kultur jaringan di laboratorium meliputi pengenalan laboratorium,alat dan bahan beserta fungsinya, multiplikasi (perbanyakan) tunas anggrek in vitro, kultur kalus, kultur embrio dan kultur mata tunas dengan sistem single node. Kegiatan pengabdian pelatihan teknik dasar kultur jaringan dapat membantu meningkatkan pengetahuan dan keterampilan peserta tentang kultur jaringan. Hal ini dapat dilihat dari antusiasme peserta selama kegiatan dan meningkatnya persentase pemahaman peserta menjadi 54,5% (tingkat pengetahuan baik), 36,4% peserta dengan tingkat pengetahuan cukup, dan 9,1% peserta dengan tingkat pengetahuan kurang.Abstract: This activity aims to improve the knowledge and skills of partners about plant tissue culture techniques. The training activity is divided into 2 sessions, namely the first session, delivering material on the basic concepts of tissue culture, equipment and material requirements, media, growth regulators, growing environmental conditions, tissue culture stages, and various in vitro culture techniques. In the second session, the practice of tissue culture techniques in the laboratory included introducing the laboratory tools and materials and their functions, multiplication (propagation) of orchid shoots in vitro, callus culture, embryo culture, and organ culture using a single node system. Basic technique training of plant tissue culture can help increase participants' knowledge and skills about tissue culture. This can be seen from the participants' enthusiasm during the activity and the increase in the percentage of participants' knowledge to 54,5% (good level of knowledge), 36,4% of participants with a sufficient level of knowledge and 9,1% of participants with a low level of knowledge.
Identification of Morphological Characters and Time of Mitotic Musa Paradisiaca cv. Haji Rina Kurnianingsih; Siti Rosidah; Dinda Sekar Ayu; Eka S Prasedya; Sri Puji Astuti
Jurnal Biologi Tropis Vol. 21 No. 3 (2021): September - Desember
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v21i3.3141

Abstract

Musa paradisiaca cv Haji is a local banana of Lombok that has a longer shelf life. The determination of the haji banana genome still uses a scoring method based on morphological character. Clarification of ploidy levels and genome types can be done using chromosome analysis. The initial information needed to perform the karyotype analysis is identifying the cleavage time of M. paradisiaca cv Haji. Banana Haji on the island of Lombok has a different local name, so morphological identification of the haji banana accession is required. Morphological observations were conducted on three accessions of Haji bananas from different cultivation sites. Identification of the time of mitosis cleavage is made by the Squash method. The results showed a uniform synapomorphic character in the haji banana accession. The characters include the aspects of pseudostem (normal), shiny pseudostem, imbricate young bractea (young bractea slightly stacked), red inner bractea, straight ovary, and no pigmentation on the stylus. The autapomorphic character of Haji banana accession in this study included inside the pseudostem of the red-green, the position of horizontal fruit bunches, a fair amount of wax on the surface of the lower leaf, and the petiole of the lower leaf in pink. There are morphological character differences in Haji cultivar accession on pseudostem color and tepal color pigmentation. The time of mitosis division in Haji bananas, especially the prophase stage - metaphase from 06.38 - 07.38 Wita. The results obtained in this study can be used as a foundation to conduct karyotype analysis of Musa paradisisaca cv Haji. 
The Effectiveness of CO2 Absorption Between Phytoplankton and Seagrass Beds in The West Sekotong Intertidal Zone of Lombok Island Sri Puji Astuti; Ika Puspita Dewi; Siti Rosidah; Sari Novida; Eka S Prasedya; Dining A Candri; Hilman Ahyadi
Jurnal Biologi Tropis Vol. 23 No. 2 (2023): April-June
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i2.4769

Abstract

The increasing sea surface temperature could be approved CO2 levels on the coast. It has been assumed the primary productivity in the intertidal zone was to absorb CO2 from the water column for photosynthesis. In addition, the substrate floor has a potential source of CO2 from decomposers. Phytoplankton and seagrasses are the primary productivity in the intertidal zone. Both of them have chlorophyll, they maintain the CO2 as a carbon source to move the photosynthetic systems to develop the metabolism of the body's cells. The measurements were made of the leaves and rhizomes of all the seagrass species. The absorption process was clarified by measuring the growth rate, percent LOI, and percent carbon in the whole body of the Tetraselmis sp and Dunaliella sp, which were phytoplankton species isolated from seagrass ecosystems. Enhalus acoroides (Ea) had the highest carbon content.  On the other hand, Halophila ovalis and Halophila minor are the lowest carbon due to the distribution of Ea being the widest, they were found in all of the sampling areas. In addition, the factor that was supported was due to its high growth rate ability. Phytoplankton and seagrass on the coast of Sekotong Barat absorb CO2 in the water column and floor of the sediment (carbon sink). The presence of a different increase in carbon content between Tetrasemis sp and Dunaliella sp indicates, the species of phytoplankton respond differently to CO2 stress in the ocean.
Co-Authors AIDA MUSPIAH Aluh Nikmatullah Dinda Sekar Ayu Dining A Candri Eka S Prasedya Hilman Ahyadi Ika Puspita Dewi Mursal Ghazali Rina Kurnianingsih Sari Novida Sri Puji Astuti