TATI NURHAYATI
Departemen Teknologi Hasil Perairan, Fakultas Perikanan dan Ilmu Kelautan, Institut Pertanian Bogor, Bogor

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Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal) Yuniarti, Tatty; Nurhayati, Tati; Jacoeb, Agoes Mardiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (383.577 KB) | DOI: 10.24002/biota.v15i3.2593

Abstract

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gelfiltration on a Sephadex G-100 column. The purification and yields were 114.731 fold and1.26% when compared to those in the starting-crude extract. The molecular mass of milkfishserine collagenase was estimated to be 14.63 kDa and 27.46 kDa.
Total Volatile Base, Glycogen, Cathepsin, and Water Holding Capacity of Tilapia (Oreochromis niloticus) on Deteoritation Phase Suhandana, Made; Nurhayati, Tati
Marinade Vol 1 No 01 (2018): Marinade
Publisher : Fisheries Product Technology Department, Faculty of Marine Science and Fisheries, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.3345/marinade.v1i01.787

Abstract

Fish was a highly perishable product. The deterioration of fish quality was followed by modification of biochemical components. These Biochemical components include a total volatile base, glycogen, cathepsin activity, and water holding capacity. The objective of this study was to determine the changes of biochemical components in fish deterioration quality. The highest TVB value showed on post rigor phase (38,40 mg N/100 g). The highest glycogen content showed on pre-rigor phase (765,1707 mg/ml). The decreasing of cathepsin activity showed on rigor mortis until post rigor phase, 0,0285 U/ml to 0,0095 U/ml. The ability fresh fish to retain water (76,39%) from total moisture content (76,89%).
Total Volatile Base, Glycogen, Cathepsin, and Water Holding Capacity of Tilapia (Oreochromis niloticus) on Deteoritation Phase Suhandana, Made; Nurhayati, Tati
Marinade Vol 1 No 01 (2018): Marinade
Publisher : Fisheries Product Technology Department, Faculty of Marine Science and Fisheries, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.3345/marinade.v1i01.829

Abstract

Fish was a highly perishable product. The deterioration of fish quality was followed by modification of biochemical components. These Biochemical components include a total volatile base, glycogen, cathepsin activity, and water holding capacity. The objective of this study was to determine the changes of biochemical components in fish deterioration quality. The highest TVB value showed on post rigor phase (38,40 mg N/100 g). The highest glycogen content showed on pre-rigor phase (765,1707 mg/ml). The decreasing of cathepsin activity showed on rigor mortis until post rigor phase, 0,0285 U/ml to 0,0095 U/ml. The ability fresh fish to retain water (76,39%) from total moisture content (76,89%).
Kandungan Proksimat Dan Mineral dari Keong Mata Lembu (Turbo setosus Gmelin 1791) Merdekawati, Dewi; Nurhayati, Tati; Jacoeb, Agoes
Jurnal Mina Sains Vol 3, No 1 (2017)
Publisher : Universitas Djuanda Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (378.773 KB) | DOI: 10.30997/jms.v3i1.865

Abstract

Keong mata lembu (T. setosus) memiliki potensi sebagai sumber protein dan mineral yang berfungsi sebagai aprodisiak seperti K, Mg, Ca, Fe, Zn, dan Cu. Penelitian bertujuan untuk mengetahui penanganan, pengolahan, dan komposisi kimia keong mata lembu. Cara panen dan penanganan keong ini masih sangat sederhana. Hasil analisis proksimat adalah 70,34% protein, 2,20% lemak, 6,87% abu, 10,06% karbohidrat, dan 10,15% air. Kandungan mineral makro tertinggi adalah kalium sebesar 8225,29 ppm sedangkan untuk mineral mikro tertinggi adalah sebesar 98,68 ppm. Kata Kunci: kandungan mineral, proksimat,Turbo setosus
Teknik Pengawetan Fillet Ikan Nila Merah dengan Senyawa Anti Bakteri asal Lactobacillus Acidophilus dan Bifido Bacteria Biffidum Saputra, Dede; Nurhayati, Tati
ComTech: Computer, Mathematics and Engineering Applications Vol 5, No 2 (2014): ComTech
Publisher : Bina Nusantara University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21512/comtech.v5i2.2358

Abstract

Red tilapia is a good commodity to be developed because it has a high nutritional value composition, with a protein content 17.8%, fat 2.8%, and others composition. The fillet of red tilapia fish is easy to spoil, because of S. aureus, Salmonella sp., and other microbes. Many methods are used to save and preserve the quality of fillet, such fillet preparation through good sanitation practices, cooling process, but the effort were not optimal. The objectives of this study were to 1) evaluate the potency of antibacterial produced by Lactobacillus acidophilus and Bifidobacteria biffidum to inhibit the growth of spoilage bacteria that contaminated the red tilapia fillet; 2) evaluate the effect of antibacterial compounds produced by Lactobacillus acidophilus and Bifidobacteria biffidum of inhibiting the setback fillet quality, 3) determine the shelf life of red tilapia fillet at room temperature. Antibacterial activity test is done by using the well diffusion method; the rate of deterioration of quality of fish tests done by observing the organoleptic parameters, pH measurement test, total volatile base method. Total number of bacteria were performed by Standard Plate Count (SPC) test. The LAB’s are able to inhibit the growth of spoilage bacteria Pseudomonas aeruginosa about 8.67-9.00 mm and Listeria monocytogenes about 8.33-9.00 mm through the well diffusion method. pH values about 5.71-5.74, TVB values about 1,26-21.43 with SPC test about 1.39-4.83 CFU/mL. The antibacterial compounds could inhibit  the rate of deterioration of quality red tilapia fillets until 14 hours.
Aktivitas Antibakteri Ekstrak Karang Lunak Sarcophyton sp. yang Difragmentasi dan Tidak Difragmentasi dari Perairan Pulau Pramuka, Kepulauan Seribu, Jakarta Kawaroe, Mujizat; Soedarma, Dedi; Effendi, Hefni; Nurhayati, Tati; Hardiningtyas, Safrina Dyah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (350.539 KB) | DOI: 10.24002/biota.v15i3.2588

Abstract

Fragmented and non-fragmented soft corals showed antibacterial activities. Soft corals were gradually extracted using methanol, ethyl acetate, and hexane. Crude extract of the samples was tested its antibacterial activity, Minimun Inhibitory Concentration, toxicity (Brine Shrimp Lethality Test method), and phytochemicals. Overall, the antibacterial activity of crude extract of non-fragmented soft coral Sarcophyton sp. was higher than the crude extract of fragmented soft coral Sarcophyton sp. Crude ethyl acetate extract showed higher antibacterial activities. The ethyl acetate crude extract of non-fragmented soft coral Sarcophyton sp. is able to inhibit all tested bacteria is E. coli, S. aureus, P. aeruginosa and B.cereus, while the ethyl acetate crude extract of fragmented Sarcophyton sp. is unable to inhibit bacteria P. aeruginosa. Minimum inhibitory concentration extracts of non-fragmented Sarcophyton sp. in range 240−480 μg/disc. The 24-h LC50 extracts of fragmented and non-fragmented Sarcophyton sp. for Artemia salina were 149.50 ppm and 45.15 ppm, respectively. Bioactive compounds of fragmented and non-fragmented Sarcophyton sp. extract are steroid, flavonoid and alkaloid.
Production of Fish Hydrolysates Protein From Waste of Fish Carp (Cyprinus Carpio) by Enzymatic Hydrolysis Saputra, Dede; Nurhayati, Tati
ComTech: Computer, Mathematics and Engineering Applications Vol 7, No 1 (2016): ComTech
Publisher : Bina Nusantara University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21512/comtech.v7i1.2201

Abstract

Fish Protein Hydrolysates (FPH) is the mixed products of polypeptide, dipeptides, and amino acid. It can be produced from materials that contained of protein by acid reaction, base reaction or enzymatic hydrolysis. The objectives of this study were to study the production of FPH from fish carp meat at post rigor phase and viscera by enzymatic hydrolysis, to determine the specific activity of papain enzyme, and to determine the solubility of FPH. Capacity of fish hydrolyzing can be identified by analyzing the content of dissolved total nitrogen (NTT) compared with nitrogen total ingredient (NTB) in order to get the value of total soluble nitrogen/total nitrogen material (NTT/NTB). The hydrolysis processes were carried out in 0,26% (w/v) papain, 60 ?C for 3 hours. The result showed that the specific activity of papain enzyme was about 3.28 U/mg. Solubility of FPH by comparing NTT/NTB was about 0.29% (fish meat) and 0.40% (fish viscera). Proximate test of protein content of fish meat was 18.34 ± 0.04 (g/100 g); while viscera was about 0.95±0.04 (g/100 g). The result indicated that product waste of fish carp had potential as a major of source of FPH.
VEGETABLE CULTIVATION TRAINING IN SUPPORTING ECONOMIC AND FOOD SECURITY IN COVID 19 PANDEMIC ERA Hayati, Nur; Nurhayati, Tati; Sodikin, Muhammad Ali; Cahyana, Nana; Marlina, Dina; Yulyana, Ade
Indonesian Journal of Devotion and Empowerment Vol 2 No 1 (2020): June 2020
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/ijde.v2i1.42284

Abstract

This training is a training on the cultivation of vegetable crops for the local community to stabilize the economy of the community during the covid-19 pandemic. The training held in Rt.05, Sindangheula Village, Banjarharjo District, Brebes Regency involved the participation of citizens while prioritizing health protocols. The purpose of this training is to improve economic conditions amid pandemic conditions that slightly disrupt the economic condition of Sindangheula villagers and educate healthy lifestyles by consuming organic vegetables of their own crops. In addition, this training is also one of the working programs of KKN BMC UNNES Year 2020. This research uses a qualitative approach. The focus in this study is how people use vegetable cultivation to anticipate food and economic problems during the Covid-19 pandemic. This research data source is obtained through primary and secondary data sources. Primary data is obtained by the authors through subject and research informants. The immediate benefit of this training activity is in addition to information and skills how to cultivate vegetables in the yard of the house and can ultimately improve economic and health conditions by producing organic vegetables from the yard of their own home. The target audience of this training activity is mothers and teenagers in Rt.05 Sindangheula Village and the trainees are 4 people. The result of this activity is that the training goes well according to the original plan and the trainees are very enthusiastic about this training and they understand how to cultivate good vegetables. Pelatihan ini adalah pelatihan budidaya tanaman sayuran bagi masyarakat setempat guna menstabilkan ekonomi masyarakat selama pandemic covid-19. Pelatihan yang di laksanakan di Rt.05, Desa Sindangheula, Kecamatan Banjarharjo, Kabupaten Brebes ini melibatkan partisipasi warga dengan tetap mengutamakan protokoler kesehatan. Tujuan pelatihan ini adalah untuk meningkatkan kondisi ekonomi di tengah kondisi pandemic yang sedikit banyak mengganggu kondisi ekonomi warga desa Sindangheula dan mengedukasi pola hidup sehat dengan mengkonsumsi sayuran organic hasil panen sendiri. Selain itu pelatihan ini juga merupakan salah satu program kerja KKN BMC UNNES Tahun 2020. Penelitian ini menggunakan pendekatan kualitatif. Fokus dalam penelitian ini adalah bagaimana masyarakat memanfaatkan budidaya sayuran untuk mengantisipasi masalah pangan dan ekonomi di masa pandemic Covid-19. Sumber data penelitian ini diperoleh melalui sumber data primer dan sekunder. Data primer diperoleh penulis melalui subjek dan informan penelitian. Manfaat langsung dari kegiatan pelatihan ini ialah sebagai tambahan informasi dan keterampilan cara membudidayakan sayuran di pekarangan rumah dan pada akhirnya dapat menyejahterakan kondisi ekonomi dan kesehatan dengan menghasilkan sayuran organic dari pekarangan rumah sendiri. Khalayak sasaran dari kegiatan pelatihan ini adalah ibu-ibu dan remaja di Rt.05 Desa Sindangheula dan peserta pelatihan berjumlah 4 orang. Hasil dari kegiatan ini adalah pelatihan berjalan dengan baik sesuai rencana awal dan peserta pelatihan sangat antusias dengan adanya pelatihan ini dan mereka memahami bagaimana cara membudidayakan sayuran yang baik.