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Analisis Kadar Total Flavonoid dan Fenolik dari ekstrak air kombinsi daun papasan (Coccinia grandis L) dan buah belimbing wuluh (Averrhoa blimbi L) Ate, Olyn Tien; Putra, I Made Wisnu Adhi; Kusumawati, I Gusti Ayu Wita; Nursini, Ni Wayan
Jurnal Media Sains Vol 3, No 2 (2019): JURNAL MEDIA SAINS
Publisher : LPPM Universitas Dhyana Pura

ABSTRAKPenelitian ini bertujuan untuk membuat ekstrak air dari kombinasi daun papasan dan buah belimbing wuluh. Selanjutnya total flavonoid dan total fenolik ekstrak diuji. Ekstrak dibuat dengan merebus kombinasi serbuk daun papasan dan buah belimbing wuluh pada berbagai rasio massa (1:0, 1:3, 1:1, 3:1, dan 0:1) dan jumlah sampel serbuk 1-5 g. Proses ekstraksi dilakukan dalam 100 ml air mendidih selama 5 menit. Hasil penelitian menunjukkan bahwa kandungan flavonoid total tertinggi dan kadar fenolik total 135,1?0,18 mg QE/g bahan kering dan 48,5?3,09 mg GAE/g bahan kering, ditemukan dalam sampel dengan rasio kombinasi 1:3 (1 g). Secara umum, semua kombinasi daun papasan dan buah belimbing wuluh memiliki jumlah flavonoid dan fenolik yang besar dan memiliki potensi besar sebagai minuman kesehatan antidiabetik.Kata kunci: Papasan, belimbing wuluh, total flavonoid, total fenolikABSTRACTCoccinia grandis L and Averrhoa blimbi L have been known to have a great activity to treat type 2 diabetes mellitus. This study was aimed to make water extracts from the combination of Coccinia grandis L and Averrhoa bilimbi L. The total flavonoid and total phenolic content of the extracts was then tested. The extracts were made by brewing the combination of powdered Coccinia grandis L leaves and Averrhoa bilimbi L fruits at various mass ratios (1:0, 1:3, 1:1, 3:1, and 0:1) and amount of powdered samples (1-5 g). The process was done in 100 ml of boiling water for 5 minutes. The results showed that the highest total flavonoid content and total phenolic content of 135.1?0.18 mg QE/g d.m. and 48.5?3.09 mg GAE/g d.m., respectively, were found in the sample with combination ratio of 1:3 (1 g). This results revealed that, generally, all combinations of Coccinia grandis L leaves and Averrhoa bilimbi L fruits have a great amount of flavonoid and phenolic and have a great potential as antidiabetic health beverages.Keywords: Coccinia Grandis L, Averrhoa Bilimbi L, total flavonoid, total phenolic

Efektifitas sinar ultraviolet terhadap cemaran bakteri patogen pada makanan cair sonde untuk pasien immune-compremissed Sulatri, Ni Luh; Yogeswara, Ida Bagus Agung; Nursini, Ni Wayan
Jurnal Gizi Indonesia (The Indonesian Journal of Nutrition) Vol 5, No 2 (2017)
Publisher : Department of Nutrition Science, Faculty of Medicine, Universitas Diponegoro

Background : Food safety remained as critical concern to immune-compremissed patient. Food safety assurance can be achieved through inhibition of pathogenic bacteria by physical treatment such as UV light radiation. However, a study regarding the effect of UV light on growth of pathogenic bacteria in contaminated liquid food are scarce.Objective : To determine the effectiveness of UV light on contamination of pathogenic bacteria in liquid food for immune-compremissed patient.Methods : Randomized design with two factor which were holding time for 60 and 120 minutes and radiation exposure (0, 5, 10 and 15 minutes). The data was analyzed using ANOVA Result : The viscosity and pH of liquid foods were 20 centipoise and 7,15 respectively. Radiation of UV light on contaminated food that have been incubated for 60 and 120 minutes at 37oC showed significant increase (1-2 log cycle) on growth of pathogenic bacteria.Conclusion : Radiation of UV light on contaminated liquid food were not effective to inhibit or kill pathogenic bacteria during holding time (60 and 120 minutes).

VIABILITAS DAN STABILITAS BAKTERI PROBIOTIK L. acidophillus FNCC 0051 PADA SUSU KEDELAI FERMENTASI SELAMA DI SALURAN CERNA IN VITRO DAN PENYIMPANAN Yogeswara, Ida Bagus Agung; Kusumawati, I Gusti AYu Wita; Nursini, Ni Wayan
Prosiding Seminar Nasional MIPA 2014: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2014
Publisher : Prosiding Seminar Nasional MIPA

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Bakteri asam laktat merupakan mikroorganisme yang berperan penting dalam fermentasi pangan dan memberikan efek kesehatan terhadap yang mengkonsumsi. Untuk menjadi organisme probiotik, bakteri asam laktat harus bertahan selama di saluran cerna dan memiliki stabilitas dan viabilitas yang baik selama penyimpanan. Tujuan dari penelitian ini adalah untuk mempelajari viabilitas dan stabilitas L. acidophillus FNCC 0051 pada susu kedelai fermentasi selama di saluran cerna in vitro dan penyimpanan. Strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama 10 hari penyimpanan pada suhu 50 C dan 250 C. Strain L. acidophillus FNCC 0051 mengalami penurunan 1,9 log cycle pada simulated gastric juice selama 10 hari penyimpanan dan di fase simulated intestinal juice. Nilai total asam dan pH susu kedelai fermentasi menunjukkan peningkatan yang signifikan selama 10 hari penyimpanan yaitu 2.5% dan 3,4 berturut turut. Penelitian ini menunjukkan bahwa strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama di saluran cerna dan penyimpanan.Kata-kata kunci: probiotik, susu kedelai fermentasi, penyimpanan, simulasi saluran cerna in vitroAbstract: Lactic acid bacteria as probiotic organism play an important role in food fermentation and gives beneficial effect to the host. In order to be a probiotic organism, lactic acid bacteria must survive through the digestive tract and has a good stability and viability during storage and under simulated gastrointestinal conditions. The aim of this research is to investigate viability and stability of L. acidophillus FNCC 0051 in fermented soymilk during storage. L. acidophillus FNCC 0051 in fermented soymilk has a good viability and stability during 10 days of storage at 50 C and 250 C. L. acidophillus FNCC 0051 decreased 1,9 log cycle in simulated gastric juice during 10 days of storage as well as in simulated intestinal juice. The acidity and pH of fermented soymilk exhibited a significant increase during 10 days of storage i.e. 2.5% and 3.4 respectively. This research has shown that L. acidophillus FNCC 0051 has a good viability and stability in fermented soymilk during storage and under simulated gastrointestinal conditions.Keyword: probiotic, fermented soymilk, storage, simulated gastrointestinal juice

Eksopolisakarida dari Lactobacillus sp. Isolat Susu Kuda Sumbawa dan Potensinya sebagai Prebiotik (EXOPOLYSACCHARIDES FROM LACTOBACILLUS SP. ISOLATED FROM SUMBAWA MARE’S MILK AND ITS POTENTIAL APPLICATION AS PREBIOTICS) I Nengah Sujaya; Ni Putu Desy Aryantini; Ni Wayan Nursini; Cok. Istri Dewiyani Cakrawati; Ni Luh Made Ema Juliasari; Ni Made Utami Dwipayanti; Yan Ramona
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research aimed to isolate exopolysaccharides (EPS) producing Lactobacilli isolated fromsumbawa mare’s milk and its potential as prebiotics for modulating the growth of Bifidobacteriumbreve. Nine strains of Lactobacillus sp. were screened for their capabilities to produce EPS usingmodified MRS medium containing sucrose. Prebiotics potential of the EPS was verified by culturingB. breve JCM1273 in TOS medium containing EPS. The results showed that all strains ofLactobacillus sp. produced EPS on MRS sucrose medium and two strains (Lactobacillus SK3.1 andLactobacillus SK4) produced more EPS compared to the other strains tested. Bifidobacteriumbreve JCM1273 showed weak activity while in direct metabolism of EPS produced by Lactobacillussp. SK4 and its growth was enhanced on acid hydrolyzed EPS. Since this phenomenon mighthappened when the EPS exposed by the low pH during gastric passage, hence the EPS might be apotential source to be developed as prebiotics. Nevertheless, further investigation is necessary toevaluate the bifidogenic affects of EPS in Lactobacillus sp. SK4.

Identifikasi dan Karakterisasi Bakteri Asam Laktat Isolat Susu Segar Sapi Bali (IDENTIFICATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM BALI CATTLE’S RAW MILK) I Nengah Sujaya; Komang Ayu Nocianitri; Ni Putu Desy Aryantini; Wayan Nursini; Yan Ramona; Yoshitake Orikasa; Fukuda Kenji; Tadashu Urashima; Yuji Oda
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Bali cattle is an indigenous spesies in Bali, which pay great attention due to its uniqueness. Numerousarticles have been published on Bali cattle especially related to its disease, nutritional requirement forgrowth and domestication. Nevertherless, it was no any report has been published on the lactic acidbacteria (LAB) assosiated with the cattles raw milk and its potential used as probiotic. This work isaimed to identify LAB isolated from bali cattle raw milk and its resistance to secondary bile acid (sodiumdeoxy cholic), a prequisite in development of probiotic for human. The results revealed that based upon thehomology studies of the variable region I, II, and III sequences of the 16S rDNA showed that 44 out of 62isolates were closely related to Pediococcus acidilactici; 11 out of 62 isolats were closely related to Enterococusgallinarum, five out of 62 isolates were closely related to Lactococcus garvieae, while only one isolate was closely related to Lactobacillus plantarum and Weisella confusa. Some isolates showed resistant to 0.2-0.6mM deoxy cholic acid, which might be also resist in human gastrointestinal tract conditions. Based onthose finding, it can be concluded that the LAB associated with raw bali cattle milk were closey related toP. acidilactici, E. gallinarum, Lac. garvieae, Lb. plantarum and W. confusa, which different from thosecommonly LAB found in others cattle raw milk. Somes isolates were potential to be developed as probioticfrom human helath.

CHARACTERIZATION OF LACTIC ACID BACTERIA ISOLATED FROM SUMBAWA MARE MILK Nengah Sujaya; Yan Ramona; Ni Putu Widarini; Ni Putu Suariani; Ni Made Utama Dwipayanti; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 2 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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A study was carried out to isolate and characterize lactic acid bacteria (LAB) from the Sumbawa mares milk The Isolation of LAB was conducted in Man Rogosa Sharpe (MRS) agar. The isolates were characterized by standard methods, such as Gram staining, cell morphology study and fermentation activities. The ability of the isolates to inhibit some pathogenic bacteria was studied by dual culture assay. Isolates showing the widest spectrum of inhibiting pathogenic bacteria were further identified using API 50 CHL. The results showed that Sumbawa mare milk was dominated by lactobacilli and weisella/leuconostoc. As many as 26 out 36 isolates belong to homofermentative lactobacilli and another 10 isolates belong to both heterofermentative lactobacilli and weissella or leuconostoc. Twenty four isolates inhibited the growth of Escherichia coli 25922, Shigela flexneri, Salmonella typhimurium, and Staphylococcus aureus 29213. Two promising isolates with the widest spectrum of inhibiting pathogenic bacteria, Lactobacillus sp. SKG34 and Lactobacillus sp. SKG49, were identified respectively as Lactobacillus rhamnosus SKG34 and Lactobacillus ramnosus SKG49. These two isolates were specific strains of the sumbawa mare milk and are very potential to be developed as probiotic for human.

PROBIOTIC POTENCY OF LACTOBACILLUS SPP. ISOLATED FROM SUMBAWA MARE MILK I Nengah Sujaya; I Made Utami Dwipayanti; Ni Luh Putu Suariani; Ni Putu Widarini; Komang Ayu Nocianitri; Ni Wayan Nursini
Jurnal Veteriner Vol 9 No 1 (2008)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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This research was deigned to elucidate the potency of Lactobacillus spp. isolated from sumbawa mare milk to be developed as a probiotic. Sixteen lacobacilli were screened based on their resitancy to a model of gastric juice at pH 2, 3, and 4, then followed by their resistncy to small intestional fluid model containing deoxycholic. Three lactobacilli i.e. Lactobacillus sp. SKA13, Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were found to be resistentent to gastric juice at pH 3 and 4. However, there were no lactobacilli resisted to pH 2. Lactobacillus rhamnosus SKG34 and Lactobacillus rhamnosus SKG49 were able to reach the colon even after being expossed to a model of intestinal fluid containing 0,4 mM deoxycholate and pancreatine. Therefore, these isolates have a potency to be developed as probiotic lactobacilli. Nevertherless, these lactobcailli could probably transform cholic acid into secondary bile acids, which were not expected to be found in the probiotic, and this capability is not appropriate for probiotic. This character is worthly to be studied since it has never been reported in lactobacilli.

VIABILITAS DAN STABILITAS BAKTERI PROBIOTIK L. acidophillus FNCC 0051 PADA SUSU KEDELAI FERMENTASI SELAMA DI SALURAN CERNA IN VITRO DAN PENYIMPANAN Ida Bagus Agung Yogeswara; I Gusti AYu Wita Kusumawati; Ni Wayan Nursini
Prosiding Seminar Nasional MIPA 2014: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2014
Publisher : Prosiding Seminar Nasional MIPA

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Bakteri asam laktat merupakan mikroorganisme yang berperan penting dalam fermentasi pangan dan memberikan efek kesehatan terhadap yang mengkonsumsi. Untuk menjadi organisme probiotik, bakteri asam laktat harus bertahan selama di saluran cerna dan memiliki stabilitas dan viabilitas yang baik selama penyimpanan. Tujuan dari penelitian ini adalah untuk mempelajari viabilitas dan stabilitas L. acidophillus FNCC 0051 pada susu kedelai fermentasi selama di saluran cerna in vitro dan penyimpanan. Strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama 10 hari penyimpanan pada suhu 50 C dan 250 C. Strain L. acidophillus FNCC 0051 mengalami penurunan 1,9 log cycle pada simulated gastric juice selama 10 hari penyimpanan dan di fase simulated intestinal juice. Nilai total asam dan pH susu kedelai fermentasi menunjukkan peningkatan yang signifikan selama 10 hari penyimpanan yaitu 2.5% dan 3,4 berturut turut. Penelitian ini menunjukkan bahwa strain L. acidophillus FNCC 0051 memiliki viabilitas dan stabilitas yang baik selama di saluran cerna dan penyimpanan.Kata-kata kunci: probiotik, susu kedelai fermentasi, penyimpanan, simulasi saluran cerna in vitroAbstract: Lactic acid bacteria as probiotic organism play an important role in food fermentation and gives beneficial effect to the host. In order to be a probiotic organism, lactic acid bacteria must survive through the digestive tract and has a good stability and viability during storage and under simulated gastrointestinal conditions. The aim of this research is to investigate viability and stability of L. acidophillus FNCC 0051 in fermented soymilk during storage. L. acidophillus FNCC 0051 in fermented soymilk has a good viability and stability during 10 days of storage at 50 C and 250 C. L. acidophillus FNCC 0051 decreased 1,9 log cycle in simulated gastric juice during 10 days of storage as well as in simulated intestinal juice. The acidity and pH of fermented soymilk exhibited a significant increase during 10 days of storage i.e. 2.5% and 3.4 respectively. This research has shown that L. acidophillus FNCC 0051 has a good viability and stability in fermented soymilk during storage and under simulated gastrointestinal conditions.Keyword: probiotic, fermented soymilk, storage, simulated gastrointestinal juice

Pemberdayaan Menu Sebagai Upaya Pengentasan Gizi Buruk Dan Obesitas Balita Di Desa Catur, Kintamani, Bangli, Bali Ni Ketut Wiradnyani; Ni Wayan Nursini; I Gede Mustika; Ida Bagus Agung Yogeswara; I Gusti Ayu Wita Kusumawati; Ni Putu Eny Sulistyadewi; Purwaningtyas Kusumaningsih; Dylla Hanggaeni Dyah Puspaningrum
Bhakti Persada Jurnal Aplikasi IPTEKS Vol 7 No 1 (2021): Mei 2021
Publisher : P3M Politeknik Negeri Bali

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31940/bp.v7i1.2397

Pengabdian masyarakat prodi Gizi Universitas Dhyana Pura dilakukan di Desa Catur, Bangli Bali bertujuan sebagai upaya memberikan edukasi dan skill kepada masyarakat ibu asuh yang mempunyai anak balita, dalam rangka memperdayakan menu sebagai upaya pengentasan gizi kurang dan lebih. Program ini diberikan untuk mengatasi masalah yang diprioritaskan yaitu: 1) kurangnya pengetahuan para ibu asuh balita mengenai bahan-bahan makanan yang mempunyai potensi untuk meningkatkan maupun menurunkan berat badan, 2) keterbatasan ketersediaan alat dan kurangnya pemahaman mengenai pengolahan makanan menjadi menu yang dapat memperbaiki status gizi anak balita, 3) kurangnya pengetahuan mengenai jenis-jenis menu sebagai asupan gizi yang harus diberikan kepada anak-anak balita gizi kurang dan lebih, 4) kurangnya kemampuan mengolah makanan menjadi menu yang menarik berbasiskan bahan lokal yang sudah ada dan melimpah, 5) kurangnya ketrampilan mengolah makanan dengan menu yang sederhana dalam waktu yang sangat singkat untuk pemenuhan gizi seimbang anak asuh balita. Pengabdian dilakukan dengan metode learning by doing, memberikan materi ceramah diikuti dengan praktek pembuatan menu sebagai upaya pemenuhan gizi seimbang balita yang diikuti oleh ibu asuh balita Desa Catur. Hasil yang diperoleh adalah peningkatan pengetahuan dan skill dalam pembuatan menu gizi seimbang.

PKM Olahan Gondo di Desa Timpag, Kecamatan Kerambitan, Kabupaten Tabanan, Bali I Gusti Ayu Wita Kusumawati; Putu Wida Gunawan; Ni Wayan Nursini; Ida Bagus Agung Yogeswara
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 5 No 2 (2020): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v5i2.2313

Desa Timpag terkenal dengan penghasil gondo. Pemanfaatan gondo umumnya hanya dibuat sebagai sayur plecing tetapi oleh Ibu Ayu, gondo dapat diolah menjadi keripik yang rasanya digemari. Pengolahan yang bersifat tradisional menyebabkan keripik yang dihasilkan berminyak dan tidak tahan lama. Keripik gondo dipasarkan di warung-warung sekitar di desa Timpag. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk membantu memperbaiki kualitas dan inovasi olahan gondo, pengurusan P-IRT, mendesain kemasan yang menarik, dan ecommerce melalui pelatihan dan pendampingan. Perbaikan kualitas keripik gondo melalui pengolahan dengan penggunaan vaccum frying dan pengeringan menggunakan spinner. Inovasi olahan gondo berupa puding gondo dan keripik gondo dengan variasi rasa (barbeque, keju, balado dan jagung manis). Kemasan digunakan plastik klip tebal dengan direkatkan menggunakan sealer, yang kemudian diberi label. Untuk memperluas area pemasaran produk dilakukan secara e-commerce melalui website. Hasil yang didapat setelah dilakukan pelatihan dan pendampingan terjadi peningkatan kualitas dan inovasi olahan gondo, mitra mempersiapkan pengurusan P-IRT, kemasan dan label yang menarik, area pemasaran yang semakin luas sehingga berdampak dengan peningkatan omset dan pemasukan Ibu Ayu.

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