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Efficacy of bivalent vaccine against black body syndrome (BBS) of barramundi Lates calcalifer B. Anis Nugrahawati; Sri Nuryati; Sukenda Sukenda; Rahman Rahman; Margie Brite; Tiya Widi Aditya
Jurnal Akuakultur Indonesia Vol. 18 No. 2 (2019): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3709.553 KB) | DOI: 10.19027/jai.18.2.172-181

Abstract

ABSTRACT Black body syndrome causes mass mortality in barramundi (Lates calcarifer) in Asia mariculture. This study aimed to evaluate the efficacy of bivalent vaccine derived from local isolate for black body syndrome (BBS) prevention on the Barramundi. The bacteria used in the bivalent vaccine were Pseudomonas stutzeri and Vibrio harveyi in a ratio of 50:50. Barramundi, 5‒6 cm in length, was intraperitoneally injected with bivalent vaccine and phosphate buffer saline (PBS). After a 21-day vaccination, fish was injected with pathogenic bacteria P. stutzeri, V. harveyi, the combination of P. stutzeri and V. harveyi with a dose of bacteria 107 CFU/fish. We observed relative percent survival (RPS), mortality, blood profile, antibody level, lysozyme activity, and histopathology of vaccinated fish. The result showed that vaccinated fish had higher antibody levels and lysozyme activity than control treatment (P>0.05). Vaccinated fish had RPS of 80.00%, 64.29%, 57.69%, after challenged test with P. stutzeri, V. harveyi, and combination of P. stutzeri and V. harveyi, respectively. Hemoglobin and hematocrit were not significantly different (P>0.05). However, the erythrocytes, leucocytes, and phagocytic activity were higher compared to there were higher erythrocytes, leucocytes, and phagocytic activity compared to control (P<0.05). As well as antibody level and lysozyme activity of vaccinated fish higher than control (P<0.05). In conclusion, the bivalent vaccine of P. stutzeri and V. harveyi could protect barramundi seed from BBS infection. Keywords: Lates calcalifer, bivalent vaccine, local isolate, Pseudomonas stutzeri, Vibrio harveyi                                                                                                                                                                       ABSTRAK Black body syndrome menyebabkan kematian masal pada ikan kakap putih budidaya air laut di wilayah Asia. Tujuan dari penelitian ini adalah untuk menguji efikasi vaksin bivalen untuk penanggulangan black body syndrome pada benih ikan kakap putih. Bakteri yang digunakan untuk membuat vaksin bivalen yaitu Pseudomonas stutzeri dan Vibrio harveyi dengan rasio 50:50. Ikan kakap putih berukuran 5‒6 cm diinjeksi vaksin bivalen dan phosphat buffer saline secara intraperitoneal. Setelah 21 hari pemeliharaan, benih diuji tantang dengan bakteri patogen P. stutzeri, V. harveyi, campuran P. stutzeri dan V. harveyi dengan kepadatan bakteri sebesar 107 CFU/ikan. Parameter yang diamati meliputi relative percent survival (RPS), gambaran darah, titer antibodi, aktifitas lisosim, dan gambaran histopatologi benih kakap putih. Hasil penelitian menunjukkan bahwa ikan yang divaksin memiliki nilai titer antibodi dan aktifitas lisosim lebih tinggi dibandingkan kontrol (P<0.05). Ikan yang divaksin memilki RPS 80.00%, 64.29%, 57.69%, setelah diuji tantang dengan P. stutzeri, V. harveyi, campuran P. stutzeri dan V. harveyi. Nilai hemoglobin dan hematokrit tidak berbeda nyata (P>0.05). Titer antibodi dan aktifitas lisosim ikan yang divaksin lebih tinggi dari kontrol (P<0.05). Vaksin bivalen P. stutzeri dan V. harveyi dapat melindungi benih ikan kakap putih dari infeksi BBS. Kata kunci : Lates calcalifer, vaksin bivalen, Pseudomonas stutzeri, Vibrio harveyi 
Aplikasi Vaksin Dna Koi Herpes Virus (Khv) Melalui Metode Perendaman Dengan Dosis Yang Berbeda Dalam Upaya Pencegahan Penyakit Pada Ikan Mas (Cyprinus carpio) Chairunnisa, Sekar Ayu; Prayitno, Slamet Budi; -, Sarjito; Santika, Ayi; Nuryati, Sri
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1457.163 KB)

Abstract

The purpose of this research was to determine fish clinical signs of KHV-infected and determine effectiveness of KHV DNA vaccine with immersion method to prevent koi herpes virus (KHV) infection. This research was conducted in National Center for Freshwater Aquaculture Development (BBPBAT) Sukabumi, from October 2012 to Februari 2013. The fish samples used were carp seed free KHV with size 3 – 5 cm as many as 450 animals. Five (5) treatments and 3 replications were administered in this research. The treatment were, without vaccine, vaccine at dose of 104, 105, 106, and 107 CFU/ml. Vaccination technique used was vaccination through immersion method. One of the vaccine that has been developed was KHV DNA vaccine. Eksperimental fishes were maintain for 30 days past vaccination, and then challenged with KHV and observed for 21 days. The variables measured were clinical signs and survival rate. The results of this research indicated that non vaccinated fish (A), vaccinated fish at 104(B) and 105(C). demonstered clinical signs of KHV-infected fish, while vaccinated fish at 106(D) and 107(D) visibly normal. This results indicated that vaccination using KHV DNA vaccines at 106 and 107 CFU/ml could prevent eksperimental fish from KHV-infection. PCR results showed that fish with treatments A, B and C positive infected KHV, while treatment D and E negative KHV. Survival rate (SR) at treatment A (15,56%), B (50,00%), C (53,33%), D (78,89%) and E (83,33%). For prevention of KHV, dose vaccine DNA KHV 106 and 107 CFU/ml could be used. This results supported by survival rate from treatment D (78,89%) and E (83,33%) higher than treatment A, B and C.
Potential transmission test of GP25 vaccine in normal flora bacteria of common carp culture media Sri Nuryati; , Alimuddin; Ayu Dhita Juliadiningtyas
Jurnal Akuakultur Indonesia Vol. 14 No. 1 (2015): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3103.447 KB) | DOI: 10.19027/jai.14.90-97

Abstract

ABSTRACT Koi herpesvirus (KHV) is a virus that infects common carp and koi Cyprinus carpio. KHV attacks most stadia of common carp and koi and causes mortality up to 80–95% of the population. One way to prevent the spread of KHV is  by applying DNA vaccine. This research was conducted to test potential transmission of DNA vaccine encoding glycoprotein 25 (vaccine GP25) toward normal flora bacteria of media cultivation of common carp. Bacteria was isolated from pond water of common carp and tested for their sensitivity to ampicillin. Research was performed by adding vaccine GP25 to ampicillin-sensitive bacteria at a dose of 12.5 µg/100 µL and incubated at 28 °C for 30, 60, 180, and 300 minutes then plated on media containing ampicillin. The grown bacteria cells were tested for the existence of plasmid bearing gen GP25 through bacteria colony cracking. The results of this research showed that there was no bacteria contained plasmid bearing gen GP25. Keywords: DNA vaccines, common carp, vaccine safety  ABSTRAK Koi herpesvirus (KHV) merupakan virus yang menginfeksi ikan mas dan koi Cyprinus carpio. KHV menyerang hampir semua stadia ikan mas dan koi dan menyebabkan kematian hingga 80–95% dari populasi. Salah satu cara penanggulangan penyebaran KHV adalah dengan penggunaan vaksin DNA. Penelitian ini dilakukan untuk menguji potensi transmisi vaksin DNA glikoprotein 25 (vaksin GP25) pada bakteri flora normal pada media budidaya ikan mas. Bakteri diisolasi dari kolam budidaya ikan mas dan diuji sensitivitasnya terhadap antibiotik ampisilin. Penelitian dilakukan dengan menambahkan vaksin GP25 ke larutan bakteri sensitif ampisilin dengan dosis 12,5 µg/100 µL dan diinkubasi pada suhu 28 °C selama 30, 60, 180, dan 300 menit kemudian disebar pada media mengandung antibiotik ampisilin. Sel bakteri yang tumbuh diuji untuk mendeteksi keberadaan plasmid pembawa gen GP25 dengan menggunakan metode seleksi koloni bakteri (colony cracking). Hasil penelitian ini menunjukkan bahwa tidak ada bakteri yang mengandung plasmid pembawa gen GP25. Kata kunci: keamanan vaksin, ikan mas, vaksin DNA
Dietary ambon lumut banana stem extract Musa cavendishii var. dwarf Paxton as an immunostimulant for white spot disease prevention in Pacific white shrimp Litopenaeus vannamei Afriani Ramadhan; Sri Nuryati; Nur Bambang Priyoutomo; Alimuddin Alimuddin
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3396.038 KB) | DOI: 10.19027/jai.16.2.164-173

Abstract

ABSTRACT This study was aimed to evaluate the dietary ambon lumut banana Musa cavendishii var. dwarf Paxton stem extract on the immune responses, growth, and survival rate of Pacific white shrimp against white spot disease. Ambon banana steam extract was obtained by maceration method using ethanol. The shrimps fed by pellet containing ambon banana stem extracts with different dosages for 29 days, i.e designated as 0.1 (A); 0.3 (B), and 0.5 (C) g/kg, the diet treatment without ambon banana stem extract without challenged test (K-), and diet treatment without ambon banana stem extract with challenged test (K+). Each treatment consisted of three replications. Feeding was conducted for 29 days of maintenance (four times a day). The results showed that the immune responses (average total hemocyte count: 45.15×106 cells/mL, phenoloxidase activity 1.03±0.08 OD, respiratory burst 0.95±0.04 OD, phagocytic activity 94.33±1.53%, growth (specific growth rate: 7.79±0.06%/day, feed ratio conversion was 52±0.01), and survival of treatment C (survival rate 100%) were higher compared with the treatment K+ (total hemocyte count: 3.83×106 cells/mL, phenoloxidase activity 0.04±0.01 OD, respiratory burst 0.18±0.06 OD,  phagocytic activity 5.67±0.58%, specific growth rate: 2.61±0.08%/day, feed conversion ratio 2.11±0.02, survival rate: 50%). Therefore, banana stem extract at a dose of 0.5 g/kg everyday diet can be used to improve growth and nonspecific immune system against white spot disease on Pacific white shrimp. Keywords: Pacific white shrimp, white spot disease, immune response, Ambon banana stem extract  ABSTRAK Penelitian ini bertujuan untuk menguji dosis optimal ekstrak batang pisang ambon melalui pakan dalam meningkatkan respon imun, pertumbuhan dan kelangsungan hidup udang putih terhadap penyakit white spot. Ekstrak batang pisang ambon lumut Musa cavendishii var. dwarf  Paxton menggunakan metode maserasi dengan menggunakan pelarut etanol. Pakan udang yang mengandung ekstrak batang pisang ambon pada dosis yang berbeda selama 29 hari 0,1 (A); 0,3 (B), dan 0,5 (C) g/kg, pakan tanpa pemberian ekstrak batang pisang ambon dan tidak diuji tantang (K-), pakan tanpa pemberian ekstrak batang pisang ambon dan diuji tantang (K+). Masing-masing perlakuan terdiri atas tiga ulangan. Frekuensi pemberian pakan empat kali sehari selama 29 hari pemeliharaan. Hasil penelitian menunjukkan bahwa perlakuan C respons imun (rata-rata jumlah total hemosit: 45,15×106 sel/mL, aktivitas phenoloxydase 1,03±0,08 OD, respiratory burst 0,95±0,04 OD, aktivitas fagositik 94,33±1,53%, pertumbuhan (laju pertumbuhan spesifik: 7,79±0,06%/hari, rasio konversi  pakan 0,52±0,01), dan kelangsungan hidup  (survival rate 100%) lebih tinggi dibandingkan dengan perlakuan K+ (jumlah total hemosit: 3.83×106 sel/mL, aktivitas phenoloxydase 0,04±0,01 OD, respiratory burst 0,18±0,06 OD, aktivitas fagositik 5,67± 0,58%, laju pertumbuhan spesifik: 2,61±0,08%/hari, rasio konversi pakan 2,11±0,02, nilai kelangsungan hidup: 50%). Jadi, ekstrak batang pisang pada dosis 0,5 g/kg setiap hari pada pakan dapat digunakan untuk meningkatkan pertumbuhan dan sistem kekebalan tubuh nonspesifik terhadap penyakit bintik putih pada udang putih.   Kata kunci: udang putih, white spot, respons imun, ekstrak batang pisang ambon
Construction of a DNA Vaccine Using Glycoprotein Gene and Its Expression Towards Increasing Survival Rate of KHV-Infected Common Carp (Cyprinus carpio) Nuryati, Sri; Alimuddin, Alimuddin; Sukenda, Sukenda; Soejoedono, Retno Damayanti; Santika, Ayi; Pasaribu, Fachriyan Hasmi; Sumantadinata, Komar
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (120.942 KB) | DOI: 10.31258/jnat.13.1.47-52

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Deoxyribonucleic acid (DNA) vaccine has recently been developed as an alternative vaccine against virus infection.This study was the first step of DNA vaccine development to protect cyprinids including common carp (Cyprinuscarpio) and fancy koi (Cyprinus carpio) from KHV (koi herpesvirus) infection in Indonesia. One of KHV glycoproteingenes, i.e. glycoprotein (GP) was ligated with Japanese medaka (Oryzias latipes) â-actin promoter to generatepAct/GP as a DNA vaccine. Fourty fish in body weight of 10-15 g/fish were individually injected by pAct/GP intomuscle in different dosage of 2.5 μg, 7.5 μg and 12.5 μg/100 μl phosphate buffer saline. Total RNA was extractedfrom the 12.5 μg of pAct/GP-injected fish muscle at 24, 48 and 67 hours post-injection to analyze GP expression byRT-PCR method. Potential of pAct/GP as DNA vaccine was examined by injecting KHV into the 30-days-vaccinatedfish. Both of possitive and negative control fish group were not vaccinated. Possitive control fish group wereinjected with KHV, but negative control fish group were not. KHV-challenged fish were reared for 1 month, and thedeath fish were calculated daily. Result of RT-PCR analysis showed that GP gene expression were detected at 3 dpost-injection. Expression of GP in the vaccinated fish groups helped to improve their survival rate after challengedby KHV. All of fish without DNA vaccination had dead 17 days after KHV injection. The results demonstrated thatpAct/GP had high potency to be used as a DNA vaccine against KHV infection in cyprinids.
Effectiveness of ambon banana stem juice as immunostimulatory against Aeromonas hydrophila infections in catfish Clarias gariepinus Qorie Astria; Sri Nuryati; Kukuh Nirmala; Alimuddin Alimuddin
Jurnal Akuakultur Indonesia Vol. 16 No. 2 (2017): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3453.412 KB) | DOI: 10.19027/jai.16.2.154-163

Abstract

ABSTRACT Outbreaks of infectious diseases due to Aeromonas hydrophila in catfish can cause high death rates (80–100%). Fish disease control can be done using phytopharmaceutical to prevent or treat diseases of fish. One of the phytopharmaceutical that known to prevent the fish diseases is ambon banana stem Musa cavendishii var. dwarf Paxton. This study was conducted to test the effectiveness of catfish immersion using banana stem juice as an immunostimulant against bacterial infections A. hydrophila. The observed parameters were a total performance of production, hematological test, and water quality. Immersion of catfish seeds with stem juice was performed at a concentration of 5 mL/L, 13 mL/L, and 21 mL/L for 30 minutes. Each treatment consisted of three replications. A  total of 15 fishes were immersed in 1.5 L water. At day-9 after immersion, fish was infected by A. hydrophila bacteria at a dose of 104 cfu/mL. The results showed that fish treated with banana stem juice at a concentration of 13 mL/L had the survival rate of 53.33±6.67% which is higher than positive control (33.33±6.67%). Based on hematology observations on the 3rd day post-challenge test (H12) with A. hydrophila, total erythrocytes, hemoglobin, total leukocytes, phagocytic activity, differential leukocyte, and lysozyme activity was highest in treatment13 mL/L. Lower feed conversion ratio (1.08±0.04) were also obtained in treatment 13 mL/L. Thus Immersion of seeds in stem juice can boost the immune system against infections A. hydrophila. Keywords: Aeromonas hydrophila, ambon banana stem juice, soaking, catfish, immune system  ABSTRAK Wabah penyakit akibat infeksi Aeromonas hydrophila pada ikan lele dapat menyebabkan tingkat kematian yang tinggi (80–100%). Penanggulangan penyakit ikan dapat dilakukan menggunakan fitofarmaka untuk mencegah ataupun mengobati penyakit ikan. Salah satu fitofarmaka yang dapat digunakan dalam upaya pencegahan penyakit ikan adalah batang pisang ambon lumut Musa cavendishii var. dwarf Paxton. Penelitian ini bertujuan untuk menguji efektivitas perendaman benih ikan lele dengan menggunakan air perasan batang pisang ambon sebagai imunostimulan terhadap infeksi bakteri A. hydrophila. Parameter yang diamati selama penelitian adalah kinerja produksi, uji hematologi dan pengukuran kualitas air. Perendaman benih ikan lele dengan air perasan  dilakukan pada konsentrasi 5 mL/L, 13 mL/L, dan 21 mL/L selama 30 menit. Pada hari ke-9 setelah perendaman, ikan diinfeksi bakteri A. hydrophila pada kepadatan 104 cfu/mL. Hasil penelitian menunjukkan bahwa perendaman ikan dengan air perasan pada konsentrasi 13 mL/L memiliki kelangsungan hidup yakni sebesar 53,33±6,67%, lebih tinggi bila dibandingkan dengan kontrol positif  (33,33±6,67%). Berdasarkan pengamatan pada uji hematologi hari ke-3 pascauji tantang (H12) dengan bakteri A. hydrophila, total eritrosit, hemoglobin, total leukosit, aktivitas fagositik, dan aktivitas lisozim tertinggi terdapat pada perlakuan konsentrasi 13 mL/L. Rasio konversi pakan yang rendah (1,08±0,04) juga terdapat pada perlakuan 13 mL/L. Hal ini menunjukkan bahwa perendaman benih ikan lele pada air perasan dapat meningkatkan sistem imun terhadap infeksi bakteri A. hydrophila. Kata kunci: Aeromonas hydrophila, air perasan batang pisang ambon, perendaman, ikan lele, sistem imun
Efficacy of GP-11 KHV DNA Vaccine in Common Carp (Cyprinus carpio) through Feed by Different Frequency of Administration Ahmad Beni Rouf; Sri Nuryati; Sukenda Sukenda; Alimuddin Alimuddin
Journal Omni-Akuatika Vol 16, No 1 (2020): Omni-Akuatika May
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.1.768

Abstract

GP-11 KHV DNA vaccine is a vaccine that can be used to induce immunity against the KHV virus (Koi herpesvirus). Vaccination through feed is an alternative way of administering vaccines. The study aimed to examine the effect of giving KHV GP-11 DNA vaccine through feed with different frequencies to KHV infection. The frequency of vaccine administration is GP-11 vaccination once a week; GP-11(1x), GP-11 vaccination twice a week; GP-11(2x), GP-11 vaccination three times a week; GP-11(3x), GP-25 vaccinations three times a week; GP-25(3x), negative control (without KHV test) and positive control (KHV tested). The fish were kept for 28 days after vaccination and then continued with the KHV challenge test for 28 days. The weight of carp ranges from 13.82±2.37 g maintained with a density of 15 fish/aquarium. The results showed that vaccine treatment was able to induce an immune response as indicated by the number of white blood cells, lysozyme activity and post-vaccination antibody titer showed a significant effect compared to controls. Likewise, after the challenge test, supported by IFNγ and IgM gene expression parameters after the challenge test showed the highest value of vaccine treatment rather than control. The efficacy of vaccine was showed by RPS value (%) in each vaccine treatment obtained GP-11(1x) value of 44.7±3.7a, GP-11(2x) of 78.9±18.2b, GP-11(3x) 85.6±12.6b and GP-25(3x) 79.5±18.1b. It was concluded that administering the GP-11 vaccine frequency 2 times a week provides protection as strong as giving a vaccine frequency 3 times a week.Keywords: common carp, DNA vaccine, frequency of administration, koi herpesvirus
Potency of Garlic Extract Against Koi Herpesvirus (KHV) in Common Carp Sri Nuryati; D. Puspitaningtyas; D. Wahjuningrum
Jurnal Akuakultur Indonesia Vol. 6 No. 2 (2007): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (139.178 KB) | DOI: 10.19027/jai.6.147-154

Abstract

Prevention of koi herpesvirus (KHV) infection using chemicals or medicines was ineffective way.  In this study garlic extract was used to prevent KHV infection.  Virus suspension of 0.1 ml and garlic extract of 0.1 ml in different dosage, i.e., 100, 200 and 300 ppt, was injected into common carp body.  Hemoglobin concentration, red and white blood cell numbers, and leukocyte number were counted.  The results of study showed that administration of 300 ppt of garlic extract could produce higher survival rate (67.5%), good blood parameters and clinical symptoms compared to other treatments. Keywords: garlic, KHV, common carp   ABSTRAK Upaya penanggulangan wabah Koi Herpesvirus (KHV) menggunakan bahan-bahan kimia atau obat-obatan adalah tidak efektif. Pada penelitian ini dilakukan pemberian ekstrak bawang putih untuk menanggulangi infeksi KHV.  Suspensi virus sebanyak 0,1 ml ditambahkan dengan 0,1 ml ekstrak bawang putih dengan berbagai konsentrasi, yaitu 100, 200 dan 300 ppt, disuntikkan ke dalam tubuh ikan mas.  Kadar hemoglobin, jumlah sel darah merah dan sel darah putih jenis dan jumlah leukosit diamati. Hasil penelitian menunjukkan bahwa pemberian ekstrak bawang putih sebanyak 300 ppt menghasilkan kelangsungan hidup (67,5%) yang lebih baik dibandingkan dengan perlakuan lainnya, dan begitu pula dengan gambaran darah serta gejala klinisnya.   Kata kunci: bawang putih, KHV, ikan mas
The Used of Paci-Paci Leaves Extract Leucas sp. to Prevent Mycotic Disease Sri Nuryati; M.A. Suparman; Y. Hadiroseyani
Jurnal Akuakultur Indonesia Vol. 7 No. 2 (2008): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (297.282 KB) | DOI: 10.19027/jai.7.205-212

Abstract

Injury of fish caused by handling of fry transportation and harvesting could impact mycosis. Effort on controlling by using chemotherapeutic could impact on pollution of environtment and even  carcinogenic effect. One of alternative substances  can be used in both preventing and controlling safely is paci-paci, Leucas sp. This method based on phytopharmacy.  The aim of this research is to test the prevention of paci-paci leaves extract to mycotic disease .  This research used five treatments: possitive control; the doses of paci-paci  extract were 0 gr/l (negative control); 0.5 gr/l; 1 gr/l and 1.5 gr/l by using the gurami, Osphronemus gouramy Lac. That was measuring 7-9 cm. The method that was used is the short-term submersion (short baths) for 24 hours.  Paci-paci extract can reduced the fungal infection and hindered the growth of Saprolegnia sp. colonies.  Descriptively, the paci-paci extract (the treatment of the extract 0.5 gr/l; 1 gr/l; 1.5 gr/l) could prevent the Saprolegnia sp. infection with the successive prevalence 33.3 %; 22.2 %; 0 %. Keyword: Osphronemus gouramy, paci-paci, Leucas sp. and mycosis   ABSTRAK Luka-luka pada tubuh ikan baik yang disebabkan penanganan pada saat pengangkutan maupun panen benih berpeluang menimbulkan penyakit mikotik yang disebabkan oleh cendawan. Upaya pengendalian menggunakan obat-obatan kimia berpotensi menimbulkan pencemaran lingkungan dan bahkan ada yang bersifat karsinogenik. Salah satu bahan alternatif yang dapat digunakan dengan aman dalam upaya pencegahan dan pengendalian tersebut adalah paci-paci, Leucas sp. yang merupakan metode berbasis fitofarmaka. Penelitian ini bertujuan untuk menguji pengaruh ekstrak daun paci-paci pada pencegahan penyakit mikotik. Penelitian ini menggunakan lima perlakuan yaitu kontrol positif; dosis ekstrak 0 gr/l (kontrol negatif); 0,5 gr/l; 1 gr/l dan 1,5 gr/l dengan menggunakan ikan gurame, Osphronemus gouramy Lac. yang berukuran 7-9 cm. Metode yang digunakan yaitu perendaman jangka pendek (short baths) selama 24 jam. Dengan metode tersebut ternyata ekstrak paci-paci dapat mengurangi timbulnya infeksi dan menghambat pertumbuhan koloni cendawan Saprolegnia sp. Ini ditunjukkan dengan semakin menurunnya nilai prevalensi dan diameter koloni jika dibandingkan dengan kontrol positif. Secara deskriptif, ekstrak paci-paci (perlakuan ekstrak 0,5 gr/l; 1 gr/l; 1,5 g/l) dapat  mencegah  serangan   Saprolegnia sp. dengan prevalensi berturut-turut 33,3 %; 22,2 %; 0 %. Kata kunci: Gurame, Osphronemus gouramy, Paci-paci, Leucas sp. dan mikotik
Identification and Koch Postsulate Test of Fungal Causative Disease in Gouramy Fish Sri Nuryati; F.B.P. Sari; . Taukhid
Jurnal Akuakultur Indonesia Vol. 8 No. 2 (2009): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.909 KB) | DOI: 10.19027/jai.8.127-133

Abstract

Micotic diseases caused by aquatic fungi is often found in gouramy fish (Osphronemus goramy Lac.) at various stages from egg hatching to adult. Samples of fungi were isolated and identified from eggs and fish indicated with fungal diseases infection. Saprolegnia was identified in infected egg whereas Aphanomyces sp. was identified in the internal part (underneath lesion) of gouramy fish. Postulate Koch tests was further confirmed that both species could infect gouramy fish.     Keyword : Gouramy, fungi, Saprolegnia and Aphanomyces   ABSTRAK Penyakit mikotik yang disebabkan oleh cendawan akuatik sering ditemui pada ikan gurame (Osphronemus goramy Lac.) dari fase penetasan telur sampai ukuran dewasa. Dari isolasi dan identifikasi yang dilakukan terhadap telur yang terinfeksi dan permukaan tukak diperoleh cendawan Saprolegnia, sedangkan isolasi dan dan identifikasi dari bagian internal (dibawah tukak) ikan gurame diperoleh cendawan Aphanomyces sp. Dari uji reinfeksi dengan menggunakan Postulat Koch diperoleh hasil bahwa cendawan Saprolegnia yang diisolasi dari telur gurame maupun cendawa cendawan Aphanomyces dari tukak dapat menginfeksi ikan gurame. Kata kunci : gurame, cendawan, Saprolegnia dan Aphanomyces
Co-Authors , Alimuddin , Alimuddin , Alimuddin , Rahman , Rusli , Sofian , Sukenda , Sukenda , Yuliyanti . Alimuddin . Alimuddin . Alimuddin . Alimuddin . Kurdianto . Sukenda . Sukenda . Sukenda . Taukhid A. Santika A. Suwanto Afriani Ramadhan Ahmad Beni Rouf Akmal Izwar Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Amalia Putri Firdausi Anang Fajrin Andi Tiara Eka Diana Puteri Angela Mariana Lusiastuti Anis Nugrahawati Arif Lukman Firmansyah Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas D. Meha D. Puspitaningtyas D. Wahjuningrum Dedi Jusadi Dedi Jusadi DEDI JUSADI Dedi Jusadi Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dian Febriani Dian Hardiantho Dinamella Wahjuningrum Dwi Hany Yanti Eddy Supriyono Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Goro Yoshizaki Hasan Nasrullah Ibnu Bangkit Bioshina Suryadi Iis Widiani Isni Rahmatika Sari Julie Ekasari Julie Ekasari K. Sumantadinata Komar Sumantadinata Kukuh Nirmala L.S. Harti Laode Muhammad Arsal Lilis Nurjanah M.A. Suparman Margie Brite Mia Setiawati Mia Setiawati Mia Setiawati Mia Setiawati Mira Mawardi Muhammad Agus Suprayudi Muhammad Zairin Jr. Muhammad Zairin Junior Muhammad Zairin Junior Munti Yuhana Muthahharah Muchtar N. Ashry N.A. Maswan Nadia Aulia Nur Bambang Priyoutomo Nurbambang Priyo Utomo Nurbariah Nurbariah Nurina Pratiwi Nurly Faridah Nurly Faridah P. Giri P. Hariyadi Qorie Astria R.D. Soejoedono Rahma Mulyani Rahman Rahman Rahman Rahman, Rahman Retno Damayanti Soejoedono Rini Purnomowati S.H. Sholeh Sarjito - Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Siti Murniasih Slamet Budi Prayitno Soko Nuswantoro Sri Murtini Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda, Sukenda Tira Silvianti Titin Kurniasih Tiya Widi Aditya Trian Rizky Febriansyah Tuti Sumiati Uttari Dewi Widanarni Widanarni Y. Kuswardani Yani Hadiroseyani Yanti Inneke Nababan Zakki Zainun