Garuda - Garba Rujukan Digital

p-Index From 2019 - 2024

4.066

P-Index

This Author published in this journals

All Journal HAYATI Journal of Biosciences Jurnal Akuakultur Indonesia Jurnal Veteriner Jurnal Perikanan Kelautan Jurnal Natur Indonesia Journal of Aquaculture Management and Technology Jurnal Ilmu-Ilmu Perikanan dan Budidaya Perairan Jurnal Ilmiah Perikanan dan Kelautan Omni-Akuatika Indonesian Aquaculture Journal Jurnal Riset Akuakultur Jurnal Iktiologi Indonesia (Indonesian Journal of Ichthyology) Journal of Aquaculture and Fish Health

Sri Nuryati

Bogor Agricultural University

Author-ID : 336092

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Environmental Science Veterinary Other

Published : 65 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4 5

Construction of a DNA Vaccine Using Glycoprotein Gene and Its Expression Towards Increasing Survival Rate of KHV-Infected Common Carp (Cyprinus carpio) Nuryati, Sri; Alimuddin, Alimuddin; Sukenda, Sukenda; Soejoedono, Retno Damayanti; Santika, Ayi; Pasaribu, Fachriyan Hasmi; Sumantadinata, Komar
Jurnal Natur Indonesia Vol 13, No 1 (2010)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat Universitas Riau

Deoxyribonucleic acid (DNA) vaccine has recently been developed as an alternative vaccine against virus infection.This study was the first step of DNA vaccine development to protect cyprinids including common carp (Cyprinuscarpio) and fancy koi (Cyprinus carpio) from KHV (koi herpesvirus) infection in Indonesia. One of KHV glycoproteingenes, i.e. glycoprotein (GP) was ligated with Japanese medaka (Oryzias latipes) â-actin promoter to generatepAct/GP as a DNA vaccine. Fourty fish in body weight of 10-15 g/fish were individually injected by pAct/GP intomuscle in different dosage of 2.5 μg, 7.5 μg and 12.5 μg/100 μl phosphate buffer saline. Total RNA was extractedfrom the 12.5 μg of pAct/GP-injected fish muscle at 24, 48 and 67 hours post-injection to analyze GP expression byRT-PCR method. Potential of pAct/GP as DNA vaccine was examined by injecting KHV into the 30-days-vaccinatedfish. Both of possitive and negative control fish group were not vaccinated. Possitive control fish group wereinjected with KHV, but negative control fish group were not. KHV-challenged fish were reared for 1 month, and thedeath fish were calculated daily. Result of RT-PCR analysis showed that GP gene expression were detected at 3 dpost-injection. Expression of GP in the vaccinated fish groups helped to improve their survival rate after challengedby KHV. All of fish without DNA vaccination had dead 17 days after KHV injection. The results demonstrated thatpAct/GP had high potency to be used as a DNA vaccine against KHV infection in cyprinids.

Aplikasi Vaksin Dna Koi Herpes Virus (Khv) Melalui Metode Perendaman Dengan Dosis Yang Berbeda Dalam Upaya Pencegahan Penyakit Pada Ikan Mas (Cyprinus carpio) Chairunnisa, Sekar Ayu; Prayitno, Slamet Budi; -, Sarjito; Santika, Ayi; Nuryati, Sri
Journal of Aquaculture Management and Technology Vol 2, No 2 (2013) : Journal of Aquaculture Management and Technology
Publisher : Journal of Aquaculture Management and Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1457.163 KB)

The purpose of this research was to determine fish clinical signs of KHV-infected and determine effectiveness of KHV DNA vaccine with immersion method to prevent koi herpes virus (KHV) infection. This research was conducted in National Center for Freshwater Aquaculture Development (BBPBAT) Sukabumi, from October 2012 to Februari 2013. The fish samples used were carp seed free KHV with size 3 â€“ 5 cm as many as 450 animals. Five (5) treatments and 3 replications were administered in this research. The treatment were, without vaccine, vaccine at dose of 104, 105, 106, and 107 CFU/ml. Vaccination technique used was vaccination through immersion method. One of the vaccine that has been developed was KHV DNA vaccine. Eksperimental fishes were maintain for 30 days past vaccination, and then challenged with KHV and observed for 21 days. The variables measured were clinical signs and survival rate. The results of this research indicated that non vaccinated fish (A), vaccinated fish at 104(B) and 105(C). demonstered clinical signs of KHV-infected fish, while vaccinated fish at 106(D) and 107(D) visibly normal. This results indicated that vaccination using KHV DNA vaccines at 106 and 107 CFU/ml could prevent eksperimental fish from KHV-infection. PCR results showed that fish with treatments A, B and C positive infected KHV, while treatment D and E negative KHV. Survival rate (SR) at treatment A (15,56%), B (50,00%), C (53,33%), D (78,89%) and E (83,33%). For prevention of KHV, dose vaccine DNA KHV 106 and 107 CFU/ml could be used. This results supported by survival rate from treatment D (78,89%) and E (83,33%) higher than treatment A, B and C.

Growth, Survival, and Body Composition of Transgenic Common Carp Cyprinus carpio 3rd Generation Expressing Tilapia Growth Hormone cDNA . Kurdianto; . Alimuddin; Nurly Faridah; Goro Yoshizaki; Sri Nuryati; Mia Setiawati
HAYATI Journal of Biosciences Vol. 23 No. 3 (2016): July 2016
Publisher : Bogor Agricultural University, Indonesia

Transgenic has been known as one of the applicable methods to improve growth performance of cultured fish. This study was performed to evaluate the growth performance, survival, and body composition of the 3rd generation of growth hormone (GH) transgenic common carp (TG). Juveniles (BW: 1.53 ± 0.03 g) were reared for 60 days in 250-L glass aquarium with stocking density of 25 fishes/aquarium. Fishes were fed with commercial feed (protein content 36%), three times a day to satiation. Growth and survival were measured every 20 days. Our results showed that TG fish has 1.49 times higher in average weight growth (p < 0.05) compared with the non-transgenic common carp (NT). Higher total feed consumption, survival, body protein content, protein and lipid retention, hepatosomatic index, and lower feed conversion ratio were also shown on TG fish compared with NT fish (p < 0.05). However, body lipid content and blood glucose level of TG fish were lower (p < 0.05) compared with the NT fish. Total ammonium nitrogen level in rearing media of TG fish was 51.78% lower (p < 0.05) than that of the NT fish. In conclusion, culturing of GH-TG common carp showed potential to achieve high productivity, efficient, and environmental-friendly aquaculture.

Behavior, Histopathology and Physiological Responses of Rat Fed Diets Containing Growth Hormone Transgenic Fish Meal Alimuddin Alimuddin; Sri Murtini; Nurly Faridah; Sri Nuryati
HAYATI Journal of Biosciences Vol. 26 No. 1 (2019): January 2019
Publisher : Bogor Agricultural University, Indonesia

The animal model response against genetically modified product may provide food safety information. This study was performed to observe behavior, histopathology and physiological responses of Wistar rat fed on the diet containing growth hormone (GH) transgenic common carp (Cyprinus carpio) meal (Ccm). Thirty rats of three-month-old (BW: 115.67–139.50g) were divided into five treatments (six rats per treatment). The treatments were rats fed on the commercial diet without Ccm (control), a re-pelleted diet containing 15% (NT-15) and 45% non-transgenic Ccm (NT-45), a re-pelleted diet containing 15% (TG-15) and 45% GH transgenic Ccm (TG-45). Rats were kept for three weeks, fed twice a day according to treatment by 30 g/day/rat and water was provided ad-libitum. Rat behavior was observed every day during feeding. Serum glutamic oxaloacetic transaminase (SGOT), serum glutamic pyvuric transaminase (SGPT), urea, and creatinine were measured at initial and the end of the experiment. Histology observation was made for liver, kidney, and brain tissues. Our results showed that behavior, histopathology, SGOT, SGPT, urea and creatinine levels of rat fed with control, NT- and TG-Ccm diets were similar. All rats survived until the end of the experiment. Thus, within the dose level of this study, GH transgenic Ccm showed no toxicity or effect on behavior and physiology of Wistar rats.

Prevention of White Spot Syndrome Virus Infection on Penaeus monodon by Immersion in CEPM Extract of Avicennia sp. and Sonneratia sp. D. Wahjuningrum; S.H. Sholeh; Sri Nuryati
Jurnal Akuakultur Indonesia Vol. 5 No. 1 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

The quality and survival rate are still being the problem that hampers the productivity of black tiger shrimp, Penaeus monodon culture. Impaired quality of larval shrimp and environmental conditions can confer shrimp be infected by diseases, including viruses such as white spot syndrome virus (WSSV). Prevention of disease infection using chemicals can offer negative impacts on water, pathogen resistance and consumers. This study was conducted to examine the efficacy of an alternative prevention compound as liquid mangrove extract (CEPM) from Avicennia sp. and Sonneratia sp. By immersion in different dose of CEPM, i.e. 250, 500, 750 and 1000 ppm, the patogenicity of WSSV was found to be different. Patogenicity of WSSV decreased after treatment by CEPM, hence this could be used to induce shrimp immunity. Optimum dose of CEPM was 250 ppm, which could increased survival rate of shrimp after challenging by WSSV, up to 98.4% shrimp survived. Keywrods: WSSV, black tiger shrimp, extract, Avicennia sp., Sonneratia sp. ABSTRAK Kualitas dan kelangsungan hidup merupakan masalah yang masih membatasi produktivitas budidaya udang windu Penaeus monodon. Kondisi udang dan kualitas lingkungan yang kurang baik dapat menyebabkan udang terserang penyakit, termasuk yang disebabkan oleh virus termasuk white spot syndrome virus (WSSV). Upaya pengendaliannya menggunakan bahan kimia secara berlebih dapat menimbulkan dampak negatif bagi lingkungan perairan, kesehatan konsumen dan menimbulkan resistensi patogen. Penelitian ini dilakukan untuk mengetahui efektivitas bahan alternatif berupa cairan ekstrak pohon mangrove (CEPM) dari jenis Avicennia sp. dan Sonneratia sp. sebagai upaya pencegahan. Dengan perendaman beberapa konsentrasi yang berbeda (250, 500, 750 dan 1000 ppm) penggunaan cairan ekstrak pohon mangrove (CEPM) Avicennia sp. dan Sonneratia sp., memberikan pengaruh yang berbeda terhadap patogenitas WSSV dan udang uji pada setiap perlakuan. Tingkat patogenitas WSSV relatif menurun setelah perlakuan tersebut sehingga dapat digunakan untuk merangsang kekebalan tubuh udang. Perlakuan yang optimal yaitu pada dosis 250 ppm, dimana pada perlakuan ini dapat meningkatkan kelangsungan hidup udang uji yang diuji tantang dengan white spot syndrome virus (WSSV) dengan tingkat kelangsungan hidup 98,4 %. Kata kunci: WSSV, udang windu, ekstrak, Avicennia sp., Sonneratia sp.

Inventarisation of Parasite in ”Dumbo” Catfish Clarias sp. from Bogor Region Y. Hadiroseyani; P. Hariyadi; Sri Nuryati
Jurnal Akuakultur Indonesia Vol. 5 No. 2 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Outbreak of parasites can reduce aquaculture productivity or even cause mass mortality of fish. Few quantities of parasite infection may still be tolerated by the host, however high intensity of infection can impair to fish metabolism or even damage of organelle. Proper treatment can be done when parasite infecting fish is known. This study was conducted to record parasite infecting "dumbo" catfish Clarias sp. that is reared by farmers in three location at Bogor, i.e. Cimanggu, Cijeruk and Cibinong. Data included prevalence and intensity of parasite were analyzed descriptively. There were 7 kinds of parasite infecting catfish from Cimanggu, i.e. Trichodina, Myxosporea, Ichthyophthirius multifiliis, Metacercaria, Gyrodactylus, Dactylogyrus and Lytocestus parvulus. Monogenean was dominant parasite infecting catfish from Cimanggu, i.e. 61% was Dactylogyrus with 83.33% of prevalence and 12.37 of intensity levels. Ichthyophthirius multifiliis, Metacercaria and Myxosporea were only found from Cimanggu samples. Catfish from Cijeruk infected by 5 kinds of parasites, i.e. Cryptobia sp., Vorticella, Gyrodactylus, Dactylogyrus and Lytocestus parvulus. Same pattern with Cimanggu, samples from Cijeruk were also dominant infected by monogenean, i.e. 46% was Dactylogyrus with 96.667% of prevalence and 20.694 of intensity levels. Samples from Cibinong were infected by 6 kinds of parasites, i.e. Vorticella, Trichodina, Gyrodactylus, Dactylogyrus, Lytocestus parvulus and Branchionus. Branchionus was not parasite, but is as ectocomensal. Parasite dominating in Cibinong samples was Branchionus by 32% with 33.333% of prevalence and 0.555 of intensity levels. Keywords: parasite, "dumbo" catfish, Clarias sp., inventarisation, Bogor ABSTRAK Parasit yang menginfeksi ikan budidaya dapat mengakibatkan menurunnya produksi bahkan kematian masal. Dalam jumlah sedikit, parasit yang menginfeksi masih dapat ditolerir oleh inang, tetapi dapat menyebabkan gangguan metabolisme bahkan kerusakan organ jika terjadi dalam intensitas yang tinggi. Dengan mengetahui jenis organisme parasit yang menyerang lele, penanggulangannya akan lebih mudah. Penelitian ini bertujuan untuk menginventarisasi parasit yang terdapat pada ikan lele dumbo Clarias sp. yang dibudidayakan oleh petani di 3 lokasi di Kabupaten Bogor yaitu Cimanggu, Cijeruk dan Cibinong. Data meliputi prevalensi dan intensitas parasit yang diperoleh dianalisa secara deskriptif. Sebanyak 7 jenis parasit yang teridentifikasi menginfeksi ikan lele dari Cimanggu, yaitu Trichodina, Myxosporea, Ichthyophthirius multifiliis, Metacercaria, Gyrodactylus, Dactylogyrus dan Lytocestus parvulus. Monogenea merupakan parasit yang mendominasi lele dari daerah ini. yaitu Dactylogyrus sebesar 61% dengan nilai prevalensi sebesar 83,333% dan intensitas sebesar 12,370. Terdapat Ichthyophthirius multifiliis, Metacercaria dan Myxosporea yang didentifikasi dari ikan sampel yang hanya berasal dari Cimanggu. Ikan sample dari Cijeruk mengandung sebanyak 5 jenis parasit yaitu Cryptobia sp., Vorticella, Gyrodactylus, Dactylogyrus dan Lytocestus parvulus. Sama halnya dengan Cimanggu, pada daerah Cijeruk juga didominasi oleh jenis monogenea yaitu Dactylogyrus sebesar 46% dengan nilai prevalensi sebesar 96,667% dan nilai intensitas sebesar 20,694. Sampel dari Cibinong ditemukan sebanyak 6 jenis parasit yaitu Vorticella, Trichodina, Gyrodactylus, Dactylogyrus, Lytocestus parvulus dan Branchionus. Branchionus diduga bukan merupakan parasit tetapi ektokomensal. Dari Cibinong didominasi oleh Branchionus sebesar 32% dengan nilai prevalensi sebesar 33,333% dan nilai intensitas sebesar 0,555. Kata kunci : parasit, lele dimbo, Clarias sp., inventarisasi dan Bogor

Effect of Bee Resin on Blood Profiles of Infected Carassius auratus by Aeromonas hydrophila Sri Nuryati; Y. Kuswardani; Y. Hadiroseyani
Jurnal Akuakultur Indonesia Vol. 5 No. 2 (2006): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Bee resin is an organic matter that can be used as immunostimulant to induce cells and tissues regeneration for fast injury recovery. In order to determine the effect of bee resin on blood profile of goldfish, Carassius auratus that had been infected by Aeromonas hydrophila, the numbers of hemoglobin, hetmaocrite, erythrocyte and leukocyte, and leukocyte differentiation were observed. Fishes were divided into four groups: negative control, positive control, preventive and curative groups. Fishes of negative control were injected intramuscularly by phosphate buffer saline 0.1 ml/fish. Control positive fishes were injected by 0.1 ml/each of 105 CFU/ml (LD50) A. hydrophila. Preventive groups were injected by bee resin of 1.5 µl/ml and then injected by 0.1 ml/each of 105 CFU/ml (LD50) A. hydrophila at the eight days after resin injection. Curative groups were injected first by 0.1 ml/each of 105 CFU/ml (LD50) A. hydrophila before injection with 3 µl/ml resin. The results of this study showed that erythrocyte number, hemoglobin and hematocrite of goldfish injected by bee resin as prevention were higher compared with positive control groups. In the curative groups, hematocryte and erythrocyte numbers was comparable with that of positive control groups. Injection of bee resin intramuscularly for 7 days increased leukocyte number and netrophyle percentage, while other parameters in other treatments were not increased. Keywords: Resin, goldfish, Carassius auratus, Aeromonas hydrophila, blood profile ABSTRAK Resin lebah merupakan salah satu bahan organik yang dapat digunakan sebagai imunostimulan serta dapat merangsang pembentukan sel dan jaringan yang mendukung proses penyembuhan luka dengan cepat. Untuk mengetahui pengaruh resin lebah terhadap gambaran darah ikan koki Carassius auratus yang terinfeksi bakteri Aeromonas hydrophila, dilakukan pengamatan kadar hemoglobin dan hematokrit, jumlah eritrosit dan leukosit serta diferensial leukositnya. Ikan dibagi ke dalam 4 kelompok; kontrol negatif, kontrol positif, preventif and kuratif. Kontrol negatif disuntik secara intramuskular dengan larutan fosfat buffer salin. Kontrol positif disuntik dengan by 0.1 ml 105 CFU/ml (LD50) bakteri A. hydrophila. Kelompok pencegahan disuntik dengan resin lebah sebanyak 1.5 µl/ml dan kemudian disuntik dengan 0.1 ml bakteri A. hydrophila 105 CFU/ml (LD50) pada hari kedelapan setelah injeksi resin. Kelompok pengobatan disuntik terlebih dahulu dengan 0.1 ml bakteri bakteri A. hydrophila 105 CFU/ml (LD50) sebelum disuntik dengan 3 µl/ml resin. Hasil pengamatan terhadap jumlah eritrosit, hemoglobin dan hematokrit ikan mas koki dengan pemberian resin lebah sebagai pencegahan menunjukkan nilai rata - rata yang lebih tinggi dibandingkan dengan kontrol positif. Pada perlakuan pengobatan, kadar hematokrit relatif lebih tinggi daripada kontrol positif dan cenderung mendekati kontrol negatif, sedang kadar hemoglobin dan jumlah eritrosit memiliki nilai yang mendekati kontrol positif. Pemberian resin lebah dengan injeksi secara intramuskular selama 7 hari meningkatkan jumlah sel darah putih dan persentase netrofil, sedangkan pada parameter yang lain (jumlah eritrosit, kadar hemoglobin, kadar hematokrit, prosentase monosit dan trombosit) pada masing - masing perlakuan umumnya tidak mengalami peningkatan. Kata kunci: Resin, ikan koki, Carassius auratus, Aeromonas hydrophila, gambaran darah

Potency of Garlic Extract Against Koi Herpesvirus (KHV) in Common Carp Sri Nuryati; D. Puspitaningtyas; D. Wahjuningrum
Jurnal Akuakultur Indonesia Vol. 6 No. 2 (2007): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Prevention of koi herpesvirus (KHV) infection using chemicals or medicines was ineffective way. In this study garlic extract was used to prevent KHV infection. Virus suspension of 0.1 ml and garlic extract of 0.1 ml in different dosage, i.e., 100, 200 and 300 ppt, was injected into common carp body. Hemoglobin concentration, red and white blood cell numbers, and leukocyte number were counted. The results of study showed that administration of 300 ppt of garlic extract could produce higher survival rate (67.5%), good blood parameters and clinical symptoms compared to other treatments. Keywords: garlic, KHV, common carp ABSTRAK Upaya penanggulangan wabah Koi Herpesvirus (KHV) menggunakan bahan-bahan kimia atau obat-obatan adalah tidak efektif. Pada penelitian ini dilakukan pemberian ekstrak bawang putih untuk menanggulangi infeksi KHV. Suspensi virus sebanyak 0,1 ml ditambahkan dengan 0,1 ml ekstrak bawang putih dengan berbagai konsentrasi, yaitu 100, 200 dan 300 ppt, disuntikkan ke dalam tubuh ikan mas. Kadar hemoglobin, jumlah sel darah merah dan sel darah putih jenis dan jumlah leukosit diamati. Hasil penelitian menunjukkan bahwa pemberian ekstrak bawang putih sebanyak 300 ppt menghasilkan kelangsungan hidup (67,5%) yang lebih baik dibandingkan dengan perlakuan lainnya, dan begitu pula dengan gambaran darah serta gejala klinisnya. Kata kunci: bawang putih, KHV, ikan mas

The use of Cattapa Leaves Terminalia cattapa as Preventive and Curative Methods in Patin Catfish Pangasionodon hypophthalmus Infected With Aeromonas hydrophila D. Wahjuningrum; N. Ashry; Sri Nuryati
Jurnal Akuakultur Indonesia Vol. 7 No. 1 (2008): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Motile Aeromonad Septicaemia (MAS) caused by Aeromonas hydrophila induced serious epidemics of ulcerative disease in freshwater fish including patin catfish Pangasionodon hypophthalmus. In vitro study for antibacterial test of cattapa leaves Terminalia cattapa (TC) were done previous to the in vivo test. The in vitro susceptibility test was performed at the dosages of 30, 60 and 90 g/l TC. At the in vivo test, fish were injected intramusculary with TC at the dosages of 60 g/l for the prevention and 120 g/l for curative efficacy. Results from blood picture, clinical sign and mortaliyt showed that TC were better and more effective as preventive than curative for MAS in patin catfish. Keywords : Terminalia cattapa, Aeromonas hydrophila, patin catfish ABSTRAK Penyakit MAS (Motile Aeromonad Septicaemia) yang disebabkan oleh Aeromonas hydrophila sering menyebabkan wabah penyakit tukak pada ikan-ikan air tawar termasuk pada ikan patin Pangasionodon hypophthalmus. Uji antibakteri secara in vitro dari daun ketapang Terminalia cattapa (TC) dilakukan sebelum uji in vivo. Pada uji in vitro dilakukan pengujian aktivitas antibakteri TC terhadap A. Hydrophila pada dosis TC 30, 60 dan 90 g/l. Pada uji in vivo, ikan diinfeksi secara intramuskular dengan TC, untuk pencegahan dengan dosis 60 g/l TC dan pengobatan pada dosis 120 g/l TC. Hasil yang diperoleh dari gambaran darah, gejala klinis dan kematian ikan patin menunjukkan bahwa TC lebih baik dan sangat efektif untuk pencegahan daripada pengobatan untuk penyakit MAS pada ikan patin. Kata kunci: Terminalia cattapa, Aeromonas hydrophila, ikan patin

The Effectiveness of Onion Extract Allium sativum to Prevent Koi Herpesvirus (KHV) Infection on Common Carp Cyprinus carpio Sri Nuryati; P. Giri; Y. Hadiroseyani
Jurnal Akuakultur Indonesia Vol. 7 No. 2 (2008): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Common carp is one of consumption fish that has delicious meat, high pritein level, and easy in farming. The serious problem in common carp farming is koi herpesvirus infection. Onion extract potency to improve immune system was estimated to prevent disease infection. The testing of the garlic extract through food could be used as efforts to increase endurance of common carp fish Cyprinus carpio to koi herpesvirus infection that was considered from blood parameter. Fish that was used was measuring 9-11 cm with the treatment of food containing 30, 50, and 70 gr/100 ml onion extract. Fish was acclimated for seven days in 60×30×30 cm3 aquarium before used. Garlic extract diet in food gave increasing of fish immune system that was infected by koi herpesvirus. The increased of leucocytes of blood fish with onion extract diet was faster than possitive control. The dose of B treatment (50 gr/100 ml) was the best dose gave short incubation periode comparing other treatment. Survival rate (SR) of this B treatment was highest, i.e. 91.7%, while survival rate of negative control was 50%. Key word: common carp, Cyprinus carpio, onion, Allium sativum, koi herpesvirus ABSTRAK Salah satu jenis ikan konsumsi air tawar yang banyak digemari oleh masyarakat adalah ikan mas Cyprinus carpio karena rasa dagingnya gurih, memiliki kadar protein tinggi dan cukup mudah dalam pemeliharaannya. Permasalahan yang muncul saat ini adalah wabah Koi Herpes Virus (KHV). Potensi ekstrak bawang putih sebagai anti mikroba spektrum luas, diduga dapat mengobati dan mencegah penyakit ikan. Pengujian bawang putih secara in vivo melalui pakan dapat digunakan sebagai upaya untuk meningkatkan ketahanan tubuh ikan mas Cyprinus carpio terhadap infeksi penyakit KHV yang ditinjau dari gambaran darahnya. Ikan uji yang digunakan adalah ikan mas berukuran 9-11 cm dengan perlakuan pakan yang mengandung bawang putih sebanyak 30, 50, dan 70 gr/100 ml. Sebelum dilakukan penelitian ikan diadaptasikan selama 7 hari pada akuarium berukuran 60×30×30 cm3. Hasil penelitian menunjukan bahwa ekstrak bawang putih efektif untuk meningkatkan ketahanan tubuh ikan mas Cyprinus carpio yang diinfeksi oleh Koi Herpes Virus (KHV). Pengamatan gambaran darah ikan yang terinfeksi KHV setelah pemberian ekstrak bawang putih selama 30 hari dapat meningkatkan jumlah leukosit lebih cepat dibandingkan dengan perlakuan kontrol positif. Peningkatan jumlah limfosit dalam darah mampu meningkatkan pertahanan tubuh. Peningkatan jumlah leukosit ditunjukkan dengan meningkatnya jumlah limfosit dan penurunan jumlah monosit. Perlakuan B (50 gr/100 ml) merupakan dosis yang paling baik karena masa inkubasi KHV terlewati lebih cepat dibanding perlakuan lain yang ditandai dengan penurunan jumlah leukositnya. Hal ini didukung oleh gejala klinis yang ringan dibandingkan kontrol positif dan tingkat kelangsungan hidup yang tinggi sebesar 91,7%. Sedangkan pada kontrol negatif kelangsungan hidupnya sebesar 50%. Kata kunci: ikan mas, Cyprinus carpio, bawang putih, Allium sativum, KHV dan Herpes

Co-Authors , Alimuddin , Rahman , Rusli , Sofian , Sukenda , Yuliyanti . Alimuddin . Kurdianto . Sukenda . Sukenda . Taukhid A. Santika A. Suwanto Afriani Ramadhan Agung Cahyo Setyawan Agus Oman Sudrajat Ahmad Beni Rouf Ahmad Muzaki Akmal Izwar Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Alimuddin Amalia Putri Firdausi Anang Fajrin Andi Tiara Eka Diana Puteri Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Angela Mariana Lusiastuti Anis Nugrahawati Ardana Kurniaji Arif Lukman Firmansyah Ayi Santika Ayi Santika Ayi Santika Ayu Dhita Juliadiningtyas Bagus Rahmat Basuki D. Meha D. Puspitaningtyas D. Wahjuningrum DEDI JUSADI Dedi Jusadi Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dendi Hidayatullah Dian Febriani Dian Hardiantho Dinamella Wahjuningrum Dinar Tri Soelistyowati Dinar Tri Soelistyowati Dwi Hany Yanti Dwi Hany Yanti Eddy Supriyono Eni Kusrini Eni Kusrini Erni Susanti F.B.P. Sari F.H. Pasaribu Fachriyan Hasmi Pasaribu Fauzan Wahib Alsani Fitriyah Husnul Khotimah Goro Yoshizaki Harton Arfah Haryanti Haryanti Hasan Nasrullah Hasan Nasrullah Ibnu Bangkit Bioshina Suryadi Iis Diatin Iis Widiani Ikhsan Khasani Indah Mastuti Irzal Effendi Irzal Effendi Isni Rahmatika Sari Julie Ekasari K. Sumantadinata Ketut Mahardika Ketut Sugama Komar Sumantadinata kukuh Nirmala Kukuh Nirmala L.S. Harti La Ode Muhammad Arsal Lili Sholihah Lilis Nurjanah M. Zairin Junior M.A. Suparman Margie Brite Mauliza Yanti Mia Setiawati Mia Setiawati Min Rahminiwati Mira Mawardi Muhammad Agus Suprayudi Muhammad Agus Suprayudi MUHAMMAD AGUS SUPRAYUDI Muhammad Zairin Jr. Muhammad Zairin Jr. Muhammad Zairin Jr. Munti Yuhana Muthahharah Muchtar N. Ashry N.A. Maswan Nadia Aulia Nur Bambang Priyoutomo Nurbambang Priyo Utomo Nurbariah Nurbariah Nurina Pratiwi Nurly Faridah Nurly Faridah Odang Carman P. Giri P. Hariyadi Putri Utami Qorie Astria R.D. Soejoedono Rahma Mulyani Rahman Rahman Rahman Rahman Rahman, Rahman Ratnawati Rifai Retno Damayanti Soejoedono Rini Purnomowati Ruku Ratu Borut S.H. Sholeh Sari Budi Moria Sembiring Sarjito - Sekar Ayu Chairunnisa Sekar Ayu Chairunnisa Sekar Sulistyaning Hadiwibowo Siti Murniasih Siti Zubaidah Slamet Budi Prayitno Soko Nuswantoro sri murtini . Suhermanto, Achmad Sukenda Sukenda . Sukenda . Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda Sukenda, Sukenda Sutiastuti Wahyuwardani Tatag Budiardi Tiara Puspa Anjani Tira Silvianti Titin Kurniasih Tiya Widi Aditya Trian Rizky Febriansyah Tuti Sumiati Tuti Sumiati Uttari Dewi Wasmen Manalu Wida Lesmanawati Widanarni Widanarni Widanarni Y. Hadiroseyani Y. Kuswardani Yanti Inneke Nababan Yonvitner - Yuni Puji Hastuti Zakki Zainun

Title

Found 65 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 65 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 65 Documents
Search