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All Journal Zuriat Jurnal Ilmu Dasar e-Journal Pustaka Kesehatan BERKALA SAINSTEK Indonesian Journal of Agricultural Science Jurnal Ilmu-Ilmu Peternakan (Indonesian Journal of Animal Science) The Journal of Pure and Applied Chemistry Research UNEJ e-Proceeding ALCHEMY Jurnal Penelitian Kimia Jurnal Abdi: Media Pengabdian Kepada Masyarakat
Ika Oktavianawati
Jurusan Kimia Fakultas Matematika Dan Ilmu Pengetahuan Alam Universitas Jember
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Education Energy Environmental Science Health Professions Languange, Linguistic, Communication & Media Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences Other

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CHANGES OF SUCROSE CONTENT AND INVERTASE ACTIVITY DURING SUGARCANE STEM STORAGE Siswoyo, Tri Agus; Oktavianawati, Ika; ., Djenal; Sugiharto, Bambang; Murdiyanto, Untung
Indonesian Journal of Agricultural Science Vol 8, No 2 (2007): October 2007
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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ABSTRACTInvertases (beta-D-fructofuranosidase, E.C. 3.2.1.26) are the keyenzymes involved in sucrose metabolism in sugarcane plants.They are highly correlated with sucrose and reducing sugar contentsduring plant growth. The sugarcane plants have two kindsof invertases, namely neutral invertase (NI) and acid invertase(AI). They have different function in sucrose accumulation.The research aimed to study the role of AI and NI in accumulationof reducing sugar during storage of sugarcane stems. Plantmaterials of 18-month-old field grown sugarcane of the commercialvariety R-579 (wet-land) and M 442-51 (dry-land) wereused. Three internodes were sampled to represent immature(internode 1-8/F1), maturing (internode 9-16/F2), and mature(internode 17-24/F3) stem tissues. All tissues were stored for0-9 days at room temperature (28-32oC) and each day, the samplewas extracted to determine invertase activity, total solubleprotein, and sugar contents. This observation was valid forinvertase activity expressed on a protein basis. At the initiateharvested (0-3 days), NI had a higher specific activity than AIin the sucrose-accumulating region of the sugarcane stems.Negative significant correlation was found between NI specificactivity and sucrose accumulation (r2 = 0.41, P < 0.05). AIshowed a higher specific activity after 4 days harvested and hadnegative correlation with sucrose accumulation (r2 = 0.40, P <0.05). These results showed that NI could be more responsiblein sucrose hydrolisis than AI at early storage of sugarcane stems.
PENGARUH VARIASI KONSENTRASI DAN pH ASAM LARUTAN SODIUM DODESIL SULFAT TERHADAP PROSES PEMISAHANNYA PADA MEMBRAN SELULOSA ASETAT Farida, Elis Nur; Indarti, Dwi; Oktavianawati, Ika
BERKALA SAINSTEK Vol 2, No 1 (2014)
Publisher : My Home

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Penelitian ini telah dilakukan dengan mempelajari pengaruh konsentrasi dan pH larutan asam surfaktan anionik natrium lauril sulfat terhadap pemisahanya pada membran ultrafiltrasi terhadap: kinerja membran selulosa asetat (fluks NaLS dan rejeksi NaLS). Preparasi membran selulosa asetat dilakukan dengan metode inversi fasa. Konsentrasi NaLS yang digunakan di bawah KKM (0,001-0,003M) dan pH asam(3-6). Hasil pengukuran KKM pada pH 3, 4, 5, 6 adalah sebagai berikut: 0,00398 M; 0,00469 M; 0,00571 M; 0,00669 M. Hasil uji kinerja membran tertinggi fluks, koefisien rejeksi dan massa teradsorb berturut-turut adalah sebagai berikut: NaLS 0,001 M pH 3 0,855 (L/m2jam); 0,003 M pH 6 99,646 %: 0,0408 g. Kata kunci: natrium lauril sulfat, konsentrasi kritis misel, selulosa asetat
The Influence of Supplementary Feeding (Probiotic and Azolla pinnata) on Protein and Amino Acids Content in Patin Fish Oktavianawati, Ika; Hermiastuti, Meirinda; Rahmawati, Novita; Handayani, Wuriyanti; Winata, I Nyoman Adi
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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One of the important factors in fish farming is feeding. Feed intake is able to affect the growth and production of fish, especially on nutritional content such as protein. In this research, Patin (Pangasius djambal), a catfish, were given variations in feed, i.e pellets only (P1) as a control; pellets and probiotic (P2); pellets and Azolla pinnata (P3). A. piñata (known locally as matalele) is a water plant that has complete essential amino acids including threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine, histidine, arginine and tryptophan. Probiotic is living microbial cells that affects the speed of feed fermentation and helps the absorption of food in the gastro intestinal tract. The results show that P2 gains more weight than P1 and P3. This feed variations also give different levels of crude protein in fish meat P1, P2, and P3 that are in the order 14.62%; 15.74%; and 13:35%. Based on the results of LC-MS analysis, the amount and the dominant amino acids content in the protein of P1, P2, and P3 are 13 amino acids–aspartic acid; 13 aminoacids–glutamic acid; and 14 amino acids and is predominantly asparagine. Keywords: protein, amino acid, patin, probiotic, Azolla pinnata
Dye-Sensitized Solar Cells (Dssc) Using Natural Dyes Extracted From Red Cabbage And Counter Electrode Based Tio2-Graphite Composites Haryati, Tanti; Mulyono, Tri; Oktavianawati, Ika; Badrianto, Wawan
UNEJ e-Proceeding Indonesian Protein Society (IPS), International Seminar and Workshop 2014
Publisher : UNEJ e-Proceeding

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Dye sensitized solar cells (DSSC) has been a popular research topic which benefits in high energy conversion efficiency and less cost in fabrication process. The components of DSSC consist of photoanode, electrolyte, and counter electrode that are arranged as sandwich. The photoanode contains ITO (indium tin oxide) glass covered by TiO2 particles, while the counter electrode contains ITO glass covered by composite of TiO2-graphite as counter electrode and red cabbage extract, as a dye agent. The cell was fabricated in various amount of graphite, i.e 10%; 30%; and 50 %, and time of soaking i.e 2; 13; 24; and 48 hours. The performance of the solar cell per active area of ​​1 cm2 was tested on irradiation of 75 Watt halogen lamp (distance 15 cm) and measured by multimeter and rheostat. The optimum synthesis condition of DSSC was obtained with composition of TiO2:graphite on the counter electrode is 70%:30 %, and soaking time in dye agent is for 24 hours. This cell results in an optimum performance, Isc = 0.028 mA ;Voc = 0.344 mV, FF = 0.29 and = 0.0055 % efficiency. Keywords: DSSC, Red Cabbage, Counter Electrode
EXPLORATION OF BROTH CHICKEN GUT AS GROWTH MEDIA OF Escherichia coli BL21 pET-Endo FOR ENDO-Î’-1,4-D-XYLANASE PRODUCTION Ratnadewi, Anak Agung Istri; Alidion, Moch. Yoris; Santoso, Agung Budi; Oktavianawatia, Ika
ALCHEMY Jurnal Penelitian Kimia Vol 13, No 2 (2017): Alchemy Jurnal Penelitian Kimia
Publisher : UNIVERSITAS SEBELAS MARET (UNS)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/alchemy.13.2.4356.191-204

Abstract

Endo-β-1,4-D-xylanase is a hydrolytic enzyme that breakdown the 1.4 chain of xylan polysaccharide. We have succes to transform the plasmid pET-Endo gene encoding endo-1,4-β-D-xylanase from Bacillus sp. originally from termites abdominal to E. coli BL21. The clone was ready for large scale of enzyme production. To reduce production cost, we look for subtitute media for the expensive Luria Berthani broth. Chicken guts broth is good alternative while rich of protein and very cheap. The content of N dissolved chicken guts broth reaches 87 % of LB broth. Growth of E. Coli BL21 in Chicken guts broth and LB broth (as control) was observed by Optical Density (OD) using spectrofotometer. Concentration of glucose added in broth and incubation temperature was varied. The result showed that optimal growth was in addition of 1.5 % glucose and incubated at  37 oC for 16 h. This optimal condition was used to grow E. coli BL21 pET-Endo for xylanase production. Enzyme purification was done by Ni-NTA affinity chromatography. Highest protein yield was 0.076 mg/mL obtained in 100 mM imidazole elucidation. The activity and specific activity of xylanase were estimated as 0.042 U/mL and 0.556 U/µg, respectively. The purification factor was 3.16 time and the molecular weight of enzyme was ± 30, 000 Dalton
Perubahan Kandungan Sukrosa dan Aktivitas Invertase pada Batang Tebu Selama Pemanenan Tri Agus Siswoyo; Ika Oktavianawati; Bambang Sugiharto; Untung Murdiyanto
Zuriat Vol 17, No 2 (2006)
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v17i2.6739

Abstract

Invertase (_-D-fructofuranosidase, E.C. 3.2.1.26) merupakan enzim kunci dalam metabolisme sukrosa pada tanaman tebu serta berkolerasi tinggi terhadap kandungan sukrosa dan gula reduksi selama masa pertumbuhan. Pada tanaman tebu terdapat 2 jenis invertase yaitu Acid Invertase (AI) dan Neutral Invertase (NI) yang mempunyai fungsi berlainan dalam pengakumulasion sukrosa. Penelitian ini bertujuan mempelajari peranan AI dan NI setelah pemanenan serta hubungannya terhadap perubahan kandungan sukrosa. Hasil penelitian menunjukkan aktivitas NI tertinggi terjadi pada awal pemanenan dan mempunyai korelasi negatif yang nyata sebesar (r2) = 0.41 dengan kandungan sukrosa. Aktivitas AI baru menunjukkan aktivitas yang meningkat setelah umur penyimpanan diperlama dan mempunyai korelasi negatif yang tinggi dengan kandungan sukrosa sebesar (r2) = 0.40. Dari hasil tersebut dapat dikatakan bahwa peranan NI lebih menentukan terhadap degradasi sukrosa pada awal pemanenan.
The Impacts of Traditional Fermentation Method on the Chemical Characteristics of Arabica Coffee Beans from Bondowoso District, East Java Ika Oktavianawati; Sattya Arimurti; Suharjono Suharjono
The Journal of Pure and Applied Chemistry Research Vol 9, No 2 (2020): Edition May-August 2020
Publisher : Chemistry Department, The University of Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jpacr.2020.009.02.526

Abstract

Bondowoso district is the predominant supplier of coffee beans, and also known as Republik Kopi. However, there was still insufficient data about the chemical characteristics of coffee from Bondowoso. This research has main aims to characterize the chemical characteristic of Coffea Arabica L. from Bondowoso, and determine the impact of traditional fermentation on them. Coffee beans were naturally-fermented through soaking in water for less than 12 hours. Unfermented coffee beans were used as a control. Both unfermented and fermented coffee beans were subjected to chemical analysis. Results show that total nitrogen and lipid contents of both fermented and unfermented beans decreased after fermentation into 0.19%±0.03 and 10.03%±0.14, respectively. LCMS analysis of coffee beans revealed that the majority of amino acid contents in fermented beans were higher than that found in unfermented beans, while caffeine and derivatives to be lower in fermented coffee beans than in unfermented beans. One exception for this was 7-methylxanthine, which was found only in fermented beans. Other metabolites, including procyanidines A and B, were found to decrease through fermentation. Interestingly, 3-flavanol was found only in fermented coffee beans. This research results would benefit on improving the quality of coffee through fermentation step.
Sintesis Asam 2-(2-(n-(2,6-diklorofenil)-4 fluorobenzamida)fenil)asetat sebagai Kandidat Obat Penghambat COX (siklooksigenase) Triodora Hutahuruk; Ayik Rosita; Ika Oktavianawati
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Sodium diclofenac is a non selectif NSAID that used as an inflammatory, analgesic, and antipyretic. Several experiment about compound as a derivate sodium diclofenac has been synthesized for reduce side effect. 2-(2-(N-(2,6-dichlorophenyl)-4-fluorobenzamido)phenil)acetat acid (N4FND) is derivate of sodium diclofenac that synthesized for development of drugs. The target compound has been synthesized by benzoylation reaction between sodium diclofenac with 4-fluorobenzoylchloride. And then, the target compound has been purified using Coloumn Chromatography. The isolate was identified using 1HNMR 400 MHz and KBr FTIR. Spectra of of 1HNM 00 MHz and KBr FTIR showed that 2-(2-(N-(2,6-dichlorophenyl)-4-fluorobenzamido)phenil)acetat acid was successfully synthesized. Pure product compound form yellowish white crystals with melting point 165-1670C.
Sintesis asam 2-(2-(4-kloro-N-(2,6-Diklorofenil)benzamida) fenil)asetat sebagai salah satu turunan diklofenak yang merupakan kandidat obat penghambat COX (siklooksigenase) (Synthesis 2-(2-(4-Chloro-N-(2,6-dichlorophenil)benzamido)phenil)acetic acid as dic Wahyu Novandari Herdiyanti; Ayik Rosita Puspaningtyas; Ika Oktavianawati
Pustaka Kesehatan Vol 2 No 3 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Sodium diclofenac is an analgetic agent as an antimetabolite that used for treating pain. Several compound as a derivative diclofenac has been synthesis for increase its activity and reduce side effect. 2-(2-(4-Chloro-N-(2,6-dichlorophenyl) benzamido)phenyl)acetic acid is a derivate of Sodium diclofenac that synthesized for development new drugs. Synthesis 2-(2-(4-Chloro-N-(2,6-dichlorophenyl) benzamido)phenyl)acetic has benzoylation reaction between Sodium diclofenac and 4-Chlorobenzoil Chloride to give the target compound (2-(2-(4-Chloro-N-(2,6-dichlorophenyl) benzamido)phenyl)acetic acid).This compound has been purified by Column Chromatography and give yellow color with melting point 143-145oC and then fix identification by H-NMR 400 MHz and KBr FTIR. 2-(2-(4-Chloro-N-(2,6-dichlorophenyl) benzamido)phenyl)acetic acid can synthesized and resulted 8.39 % of yield.   Keywords: Sodium diclofenac, 2-(2-(4-Chloro-N-(2,6-dichlorophenyl)benzamido)phenyl)acetic acid, pain.
Sintesis 1-(4-metoksibenzoiloksimetil)-5-fluorourasil sebagai Agen Antikanker . Ifada; Ayik Rosita Puspaningtyas1; Ika Oktavianawati; Yuni Retnaningtyas; Nia Kristiningrum
Pustaka Kesehatan Vol 1 No 1 (2013)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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A new compound from 5-fluorouracil (5-FU) derivatives, 1-(4-methoxybenzoyloxymethyl)-5-fluorouracil has been synthesized in a two steps reaction, alkylation (5-FU and formaldehyde to form 1-hydroxymethyl-5-fluorouracil) and esterification (1-hydroxymethyl-5-fluorouracil and 4-methoxybenzoylchloride to form 1-(4-methoxybenzoyloxymethyl)-5-fluorouracil). Reaction product purified by chromatography column, the product has been characteryzed for physical apperance is a yellowish white, crystal and melting range 168-169OC. Based on the FTIR and 1H-NMR spectra, it showed that the purified product does not contain a single compound. This fact is supported by Thin Layer Chromatography (TLC) result showing two spots for the pressure of 1-(4-methoxybenzoyloxymethyl)-5-fluorouracil and 4-methoxybenzoate acid. Keywords: 5-fluorouracil derivates, anticancer, 1-(4-methoxybenzoyloxymethyl)-5-fluorouracil, benzoylation.
Co-Authors . Ifada Achmad Sjaifullah Agung Budi Santoso Alidion, Moch. Yoris Anak Agung Istri Ratnadewi Anita Karolina Ari Satya Nugraha Arifulloh Arifulloh Ayik Rosita Puspaningtyas Ayik Rosita Puspaningtyas1 Bambang Sugiharto Bambang Sugiharto Bambang Sugiharto Bella J Zhentya Djenal . Djenal Djenal Dwi Indarti Dwi Purwita Utari Elis Nur Farida Elis Nur Farida, Elis Nur I Nyoman Adi Winata Meirinda Hermiastuti Meirinda Hermiastuti, Meirinda Nanda Letisya Nanda Letitia Ivana Nia Kristiningrum Niken Widya Palupi Novita Rahmawati Novita Rahmawati, Novita Priscillia Citra RD Ratih Sattya Arimurti Sovia MW Sholihah Suharjono Suharjono Tanti Haryati Tri Agus Siswoyo Tri Mulyono Triodora Hutahuruk Untung Murdiyanto Untung Murdiyanto Untung Murdiyanto Wahyu Novandari Herdiyanti Wawan Badrianto Wawan Badrianto, Wawan Wuriyanti Handayani Wuriyanti Handayani Wuriyanti Handayani, Wuriyanti Wuryanti Handayani Yuni Retnaningtyas