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Prevalensi Bakteri Methicillin-Resistant Staphylococcus aureus (MRSA) Pada Peralatan Laboratorium kurniawan Kurniawan -; Eva Adaning Tyas; Supriyadi -
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 4, No 2 (2021): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jmlt.v4i2.7554

Abstract

Staphylococcus aureus bacteria are common flora bacteria that are both commensal and opportunistic pathogens in humans. They are commonly treated with antibiotics, which can lead to bacterial resistance, such as the emergence of Methicillin-Resistant Staphylococcus aureus (MRSA) bacteria, which are resistant to the β -lactam antibiotics that are currently widespread to a variety of communities, such as the campus community, which is equipped with several facilities such as laboratories. The microbiology laboratory is a practicum and research laboratory that uses materials in the form of microorganisms, hence it has the potential to transmit MRSA bacteria. The aim of this study is to find out if MRSA bacteria are present on microbiology lab equipment and how prevalent they are. This study used observational research with a descriptive research design and a purposive sampling strategy. The results of bacterial isolation on MSA medium from fourteen laboratory equipment revealed that thirteen devices exhibited bacterial growth, whereas one device had none. Seven bacterial isolates with S. aureus-like features are detected growing in three of the thirteen equipment, the LAF, incubator, and manual autoclave. The seven bacterial isolates were identified as having round colony shape, glistening, opaque, convex yellow pigmentation, round cell morphology, clustered like grapes and purple in color, positive for coagulase and catalase, and resistance to methicillin antibiotics (MRSA bacteria). It can be concluded that of the fourteen-laboratory equipment examined, three instruments, namely the LAF, incubator, and manual autoclave, were found to be overgrown with MRSA bacteria, with the prevalence of MRSA bacteria in microbiology laboratory equipment reaching 21.4%.  Keywords: laboratory equipment, MRSA, prevalence, S. aureus
Pengaruh Variasi Suhu dan Lama Penyimpanan terhadap Viabilitas Bakteri Asam Laktat (BAL) dan Nilai pH Yoghurt Aditya Dimas Pangestu; Kurniawan Kurniawan; Supriyadi Supriyadi
Borneo Journal of Medical Laboratory Technology Vol 3 No 2 (2021): Borneo Journal of Medical Laboratory Technology
Publisher : Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/bjmlt.v3i2.2169

Abstract

Consuming yogurt indicates the increasing public awareness of healthy lifestyles has led many people to consume yogurt. Yogurt is a fermented milk product by adding pasteurized skim milk concentrate and adding lactic acid bacteria culture. L. casei is the bacterial culture commonly used in the production of yogurt, which has a role in breaking down lactose into lactic acid. To maintain its quality, yogurt is generally stored at a low temperature. However, there are still many business actors who only store it in a cool box or even at room temperature. The purpose of this study was to describe the viability of LAB and the effect of variations in temperature and storage time on the viability of LAB and pH value of yogurt. This study used an experimental method with a completely randomized design (CRD) with 3 treatments in the form of variations in storage temperature, namely 5°C, 15°C, and 25°C, each treatment temperature was stored for 1 week, 2 weeks, 3 weeks, and 4 weeks. This study was conducted in December 2020. The results showed that the highest viability value (289x106 CFU/mL) of lactic acid bacteria in yogurt was obtained at 5°C with two weeks of storage. Yogurt's pH value is influenced by temperature and storage time; the higher the temperature and storage time, the causes the yogurt's pH value decreases. The conclusion that can be drawn from this study is that the highest viability of LAB was obtained at 5°C with a shelf life of two weeks, while the best pH value of yogurt was achieved at 5°C for one week.
IDENTIFIKASI JAMUR PATOGEN PENYEBAB DERMATOFITOSIS PADA JARI KAKI PETANI DI DESA BOJONGSARI, BANYUMAS. Afriska Nurhidayah; Kurnia Ritma Dhanti; Supriyadi Supriyadi
Jurnal Labora Medika Vol 5, No 1 (2021): Jurnal Labora Medika
Publisher : Program Studi Teknologi Laboratorium Medik

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (393.337 KB) | DOI: 10.26714/jlabmed.5.1.2021.8-17

Abstract

Dermatofitosis merupakan penyakit yang disebabkan oleh kolonisasi jamur dermatofita pada jaringan yang mengandung keratin seperti stratum korneum kulit, rambut dan kuku pada manusia. Dermatofitosis tersebar di seluruh dunia dengan prevalensi yang berbeda-beda tiap negara dan Indonesia menempati urutan kedua. Tingginya kasus tersebut didukung dengan Indonesia merupakan salah satu negara beriklim tropis yang memiliki suhu dan kelembaban tinggi, dimana merupakan suasana yang baik bagi pertumbuhan jamur, higiene juga berperan untuk timbulnya penyakit ini, sehingga jamur dapat ditemukan hampir di semua tempat. Tujuan dari penelitian ini adalah untuk mengetahui adanya jamur patogen penyebab dermatofitosis pada kerokan kulit sela-sela jari kaki petani di Desa Bojongsari, Kecamatan Kembaran, Kabupaten Banyumas. Metode penelitian yang digunakan adalah metode deskriptif dengan teknik pengambilan sampel purposive sampling dengan jumlah sampel 8 petani. Pengambilan sampel uji berupa kerokan kulit sela-sela jari kaki petani yang menunjukkan karakteristik dermatofitosis. Berdasarkan hasil penelitian secara makroskopis (warna koloni, warna balik koloni, hifa udara, tekstur permukaan, tepi koloni, pola koloni dan diameter koloni) sedangkan pengamatan mikroskopis (spora dan hifa) menunjukkan adanya jamur Aspergillus sp. (2 isolat), Penicillium sp. (7 isolat) dan Trichophyton sp. (1 isolat). Kesimpulan dari penelitian ini, dari 8 sampel diperoleh 10 isolat jamur patogen meliputi jamur Aspergillus sp., Penicillium sp. dan Trichophyton sp.
Studi Fenomenologis Pengembangan Kesehatan Melalui Program PKH di Kecamatan Banjaranyar Kabupaten Ciamis: Phenomenological Study of Health Development Through the PKH Program in Banjaranyar District, Ciamis Regency Hikam Mika'il; Kurnia Ritma Dhanti; Supriyadi; Dita Pratiwi Kusuma Wardani
Media Publikasi Promosi Kesehatan Indonesia (MPPKI) Vol. 5 No. 7: JULY 2022 - Media Publikasi Promosi Kesehatan Indonesia (MPPKI)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Muhammadiyah Palu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56338/mppki.v5i7.2433

Abstract

Latar Belakang: Kesehatan merupakan aspek terpenting dalam kehidupan seseorang, keluhan kesehatan pada seseorang dapat menurunkan produktivitas. Data sensus keluhan kesehatan pada tahun 2021, 27 dari 100 penduduk Indonesia mempunyai keluhan kesehatan dalam sebulan terakhir dan 13 dari 100 kegiatan sehari-hari menjadi terganggu akibat keluhan kesehatan. Tujuan: Pengembangan kesehatan melalui Program Keluarga Harapan (PKH) diharapkan mampu untuk meningkatkan produktivitas masyarakat, khususnya peserta PKH. Metode: Penelitian ini menggunakan jenis penelitian kualitatif dengan pendekatan fenomenologi. Hasil: Hasil penelitian berupa komponen input, proses, dan output. Seluruh hasil penelitian diolah secara deskriptif dan disampaikan dalam bentuk narasi disertai rangkuman berbentuk tabel. Kesimpulan: Upaya pengembangan kesehatan melalui program PKH berasal dari inovasi kebijakan yang mengedepankan penyempurnaan.
Effectiveness of ginger and cinnamon compresses on pain in elderly with osteoarthritis Nur Fitriani; Supriyadi Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 1 (2020): Proceedings of the 1st International Conference of Health Science Faculty Universitas
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.362 KB) | DOI: 10.30595/pshms.v1i.26

Abstract

Osteoarthritis (OA) was one of the most common chronic diseases and a major cause of pain and disability that decreased health status. This disease caused pain and disability in patients which disturbed daily activities. Warm compress is one of the non-pharmacological therapies used to reduce pain. Warm compress therapy is applied at the sub-acute and chronic stage of osteoarthritis to reduce pain, compression and pain in the joints. Warm compresses can be combined by adding ginger and cinnamon. The aim of the reaesarh to understand the effectiveness of ginger and cinnamon compresses on lowering pain in the elderly with osteoarthritis in the orphanage of Sudagaran. This research was quasi-experimental design with two pretest-posttest design groups was used in this study. The samples were collected by using a random sampling technique with a total of 15 respondents getting ginger compress and 15 respondents getting cinnamon compress which was in accordance with the inclusion and exclusion criteria. The data were analyzed by using univariate and bivariate with Mann Whitney U Test. The results of the Mann Withney U Test on the second post-therapy obtained the difference in the value of ginger compresses with p = 0.417 and the difference in comfort value of cinnamon compresses with p = 0.127. After calculating the size effect, the ginger size effect was 0.924 while the cinnamon size effect was 0.790. The use of ginger compresses is more effective than cinnamon compresses in lowering pain in the elderly with osteoarthritis in the Community Health Center of Sudagaran.
Relationship of nutritional completeness with events of stunting in children age 1- 2 years Renaldi Gusela Wilian Nanda; Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 1 (2020): Proceedings of the 1st International Conference of Health Science Faculty Universitas
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.185 KB) | DOI: 10.30595/pshms.v1i.32

Abstract

The problem of stunting is one of the nutritional problems that the world face, especially in poor and developing countries. Stunting is a chroniac malnutrition in the period of growth and development since early life. Nutrition is an important element in the fulfilment of nutritional needs because it serves to produce energy, build and maintain tissues, and regulate life processes in the body. In addition, nutrition is related to brain development, learning ability and work productivity. Stunting or short is a condition of failure to thrive in infants (0-11 months) and toddlers (12-59 months) as a result of chronic malnutrition, especially in the first 1,000 days of life so that the child is too short for their age. The objective is to determine the relation between the completeness of nutritional stats and the incidence of stunting in children aged of 1-2 years in the working area of Community Health Center I Kembaran. This type of research was quantitative using a descriptive correlation design with a cross sectional approach. The population in this study were children under 2 years who met the criteria. The sampling technique used was simple random sampling, namely 86 respondents were included. The analysis used was the chi square statistical test. Statistics in this study consisted of frequency of completeness of nutritional status with the occurrence of stunting in children aged 1-2 years. From the results of data processing, it was found that there was a relationship between the completeness of nutritional status with the incidence of stunting in children aged 1-2 years in the working area of Community Health Center I Kembaran with a p-value of 0,000 <0.005. There was a relationship between the completeness of nutritional status with the incidence of stunting in children aged 1-2 years in the working area of Community Health Center I Kembaran.
Hubungan Umur dan Lingkar Pinggang Dengan Kadar Trigliserida pada Peserta Prolanis di Puskesmas Sumbang I Wahyu Dwi Asriani; Umi Solikhah; Supriyadi Supriyadi; Retno Sulistiyowati
ULIL ALBAB : Jurnal Ilmiah Multidisiplin Vol. 1 No. 10: September 2022
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

A decline in physical functional health and the tendency of morbidity increase in the elderly. One of which is coronary heart disease and one of the risk factors is hypercholesterolemia. Waist size is strongly correlated with central obesity and cardiovascular risk. In this case, cardiovascular disease ranks as the first cause of death in Indonesia. It is a general term that describes diseases of the heart or blood vessels. Excess levels of triglycerides in the body are called hypertriglyceridemia. The study was conducted in April-May 2022 by doing descriptive observation. There were only respondents out of 87 were analyzed using univariate and then by correlation test with the result that there is no relationship between age and triglyceride levels with Sig value. F Change (P) of 0.235, which means the value is > 0.05. In the results between waist circumference and triglyceride levels, the results of Sig. F Change (P) of 0.002 which means the value is <0.05 so it can be concluded that waist circumference with triglyceride levels has a relationship and has a strong relationship level at the R value (Correlation Coefficient) of 0.617 has a strong relationshi.
PELATIHAN DETEKSI DINI STROKE DENGAN METODE SEGERA KE RS BAGI WARGA MUHAMMADIYAH Sodikin Sodikin; Asiandi Asiandi; Supriyadi Supriyadi
PROSIDING SEMINAR NASIONAL LPPM UMP Vol 2 (2020): PROSIDING SEMINAR NASIONAL LPPM UMP 2020
Publisher : Lembaga Publikasi Ilmiah dan Penerbitan (LPIP)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (523.639 KB)

Abstract

Tujuan: kegiatan ini merupakan salah satu upaya promotif serta preventif dalam menurunkan prevalensi stroke. Tujuan kegiatan ini adalah meningkatkan pengetahuan dan kemampuan melakukan deteksi dini dari serangan penyakit sroke. Metode: Program ipteks bagi masyarakat ini dilakukan dengan metode ceramah, diskusi dan tanya jawabsimulasi/demonstrasi terkait dengan cara melakukan deteksi dini stroke. Alat evaluasi keberhasilan program ini dilakukan dengan melihat skor pengetahuan dan kemampuan detesksi dini stroke ”SEGERA KE RS” pre dan post test dari edukasi dan pelatihan. Hasil: Berdasar uji normalitas menggunakan uji Kolmogorov-Smirnov menunjukkan variabel pengetahuan tentang stroke berdistribusi normal, p = 0,173 sehingga analisis menggunakan uji t paired. Sedangkan variabel pengetahuan tentang deteksi dini stroke menggunakan slogan ”SEGERA KE RS” tidak berdistribusi normal, p <0,001 sehingga analisis menggunakan uji Wilcoxon Signed Ranks. Berdasarkan hasil program ipteks bagi masyarakat menunjukkan skor rata-rata pengetahuan peserta IbM tentang stroke sebelum pelatihan adalah 89 (dengan skor minimum-maksimum adalah 80-100) dan setelah pelatihan adalah 93,50 (dengan skor minimum-maksimum adalah 70-100). Skor rata-rata pengetahuan tentang deteksi dini stroke dengan slogan ”SEGERA KE RS” sebelum pelatihan adalah 73,20 (dengan skor minimum-maksimum adalah 0-100) dan sesudah pelatihan adalah 96,85 (dengan skor maksimum-minimum adalah 67100). Sedangkan berdasarkan analisis menunjukkan tidak adanya perbedaan yang signifikan pada pengetahuan tentang stroke antara sebelum dan sesudah pelatihan, t(df) = -1,917(19), p = 0,070, r = 0,40; untuk pengetahuan tentang deteksi dini dengan menggunakan slogan ”SEGERA KE RS” menunjukkan ada perbedaan yang si gnifikan antara sebelum dan sesudah pelatihan, z = -2,238, p = 0,025, r = -0,50. Kesimpulan: berdasarkan analisis statistik menunjukkan tidak ada perbedaan yang signifikan pengetahuan tentang deteksi dini stroke dengan slogan ”SEGERA KE RS” sebelum dan sesudah pelatihan.
Hubungan Tingkat Pengetahuan, Motivasi, dan Behaviour Skill Model dengan Perilaku Pemeriksaan Payudara Sendiri (SADARI) pada Mahasiswi Keperawatan S1 Universitas Muhammadiyah Purwokerto Pratiwi Ayuningtyas; Supriyadi Supriyadi
Proceedings Series on Health & Medical Sciences Vol. 4 (2023): Proceedings of the Midwifery Conference on Collaborative Maternity Care (DYNAMIC)
Publisher : UM Purwokerto Press

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30595/pshms.v4i.569

Abstract

Latar Belakang: Pemeriksaan payudara sendiri (SADARI) merupakan metode deteksi dini kanker payudara pada wanita yang dilakukan sedini mungkin. Di Indonesia, kanker payudara merupakan penyakit tertinggi dengan prevalensi sebesar 16,6% (65.858) kasus.Tujuan: Mengetahui hubungan tingkat pengetahuan, motivasi, dan behaviour skills model dengan perilaku pemeriksaan payudara sendiri (SADARI) pada mahasiswi Keperawatan S1 di Universitas Muhammadiyah Purwokerto.Metode: Penelitian ini merupakan penelitian kuantitatif dengan pendekatan cross sectional. Sampel penelitian ini adalah mahasiswi Keperawatan S1 semester 4 dengan jumlah 58 responden dengan metode simple random sampling pada bulan Januari-Februari 2022. Instrumen penelitian ini menggunakan lembar kuesioner dengan uji Chi-Square. Hasil: Hasil penelitian didapatkan 34 responden (58,6%) perilaku SADARI buruk, 55,2% mahasiswi dengan tingkat pengetahuan rendah, 53,4% mahasiswi dengan motivasi rendah, dan 55,2% mahasiswi dengan behaviour skills model tidak terampil. Ada hubungan tingkat pengetahuan dengan perilaku SADARI (p-value= 0,000), ada hubungan motivasi dengan perilaku SADARI (p-value= 0,002), dan ada hubungan behaviour skills model dengan perilaku SADARI (p-value= 0,001).Kesimpulan: Ada hubungan antara tingkat pengetahuan, motivasi, dan behaviour skills model dengan perilaku SADARI.
Hubungan Umur dan Lingkar Pinggang Dengan Kadar Trigliserida pada Peserta Prolanis di Puskesmas Sumbang I Wahyu Dwi Asriani; Umi Solikhah; Supriyadi Supriyadi; Retno Sulistiyowati
ULIL ALBAB : Jurnal Ilmiah Multidisiplin Vol. 1 No. 10: September 2022
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (165.547 KB)

Abstract

A decline in physical functional health and the tendency of morbidity increase in the elderly. One of which is coronary heart disease and one of the risk factors is hypercholesterolemia. Waist size is strongly correlated with central obesity and cardiovascular risk. In this case, cardiovascular disease ranks as the first cause of death in Indonesia. It is a general term that describes diseases of the heart or blood vessels. Excess levels of triglycerides in the body are called hypertriglyceridemia. The study was conducted in April-May 2022 by doing descriptive observation. There were only respondents out of 87 were analyzed using univariate and then by correlation test with the result that there is no relationship between age and triglyceride levels with Sig value. F Change (P) of 0.235, which means the value is > 0.05. In the results between waist circumference and triglyceride levels, the results of Sig. F Change (P) of 0.002 which means the value is <0.05 so it can be concluded that waist circumference with triglyceride levels has a relationship and has a strong relationship level at the R value (Correlation Coefficient) of 0.617 has a strong relationshi.