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Deteksi dan Identifikasi Cemaran Virus Avian Influenza pada Pasar Tradisional di Kabupaten Aceh Besar dan Kota Banda Aceh Helmi, Teuku Zahrial; Yulisma, Rika; Panjaitan, Budianto; Tabbu, Charles Rangga; Haryanto, Aris
Jurnal Sain Veteriner Vol 33, No 2 (2015): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (560.859 KB) | DOI: 10.22146/jsv.17920

Abstract

Penelitian ini dirancang untuk mendeteksi keberadaan virus AI pada beberapa titik kritis, yaitu unggas, kandang penampungan sementara, tempat pemotongan dan penjualan karkas, dan mengidentifikasi subtipe virus AI pada beberapa pasar tradisional di Kabupaten Aceh Besar dan Kota Banda Aceh, Propinsi Aceh. Sampel swab diambil secara acak (random) dari empat pasar di Kabupaten Aceh Besar dan Kota Banda Aceh, Propinsi Aceh, yaitu pasar Lambaro, Ketapang, Ulekareng dan Peunayong, dan dipoolkan. Satu pool sampel berisi 1 sampai 5 swab yang dikelompokkan berdasarkan pedagang, jenis unggas, dan titik kritis cemaran AI. Jumlahtotal sampel yang diambil adalah 314 swab, yang terdiri dari swab trakea unggas hidup, kandang penampungan, meja tempat pemotongan, dan karkas, kemudian dikelompokkan dalam 121 pool. Isolasi virus AI kedalam telur ayam bertunas (TAB), pemeriksaan secara serologis (uji HA/HI), dan identifikasi molekuler (metode RT-PCR) di lakukan di bagian Virologi Laboratorium Veteriner, dinas Kesehatan Hewan dan Peternakan Provinsi Aceh, dan Laboratorium Riset Terpadu Fakultas Kedokteran Hewan Universitas Syiah Kuala Banda Aceh. Analisis hasil pemeriksaan serologis dan identifikasi molekuler virus AI dilakukan dengan metode deskriptif. Hasilisolasi virus pada TAB menunjukkan bahwa embrio mengalami kematian pada 1 hari setelah di infeksikan dengan material virus dari sampel swab yang berasal dari pasar Lambaro Kabupaten Aceh Besar, dan titer antibodi terhadap virus AI hanya terdeteksi pada itik dan ayam pedaging yang berkisar antara 24 sampai dengan 27. Dari hasil serologis tersebut dilanjutkan dengan pemeriksaan RT-PCR. Berdasarkan hasil uji RT-PCR menggunakan primer spesifik terhadap gen M, H5, dan N1 diperoleh virus AI yang bersirkulasi pada unggas di pasar Lambaro tergolong subtipe H5N1. Hasil penelitian ini membuktikan bahwa diantara beberapa titik kritiscemaran virus AI di pasar tradisional, unggas hidup (itik dan ayam pedaging) yang berada dalam kandang penampungan sementara merupakan potensial penyebaran virus AI ke lingkungannya.
DENSITAS RADIOGRAFI TULANG HUMERUS ANJING LOKAL (Canis lupus familiaris) YANG DI OVARIOHISTEREKTOMI. (Radiography Density Of Humerus Bone On Local Ovariohisterektomi Dogs (Canis lupus familiaris). morow, meutya silviana; panjaitan, budianto; syafruddin, syafruddin; masyitha, dian; erwin, erwin; tasmi, cut nila
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.168 KB) | DOI: 10.21157/jim vet..v2i3.7907

Abstract

ABSTRAKPenelitian ini dilakukan bertujuan untuk mendapatkan gambaran densitas radiografi tulang humerus pada anjing lokal (Canis lupus familiaris) sebelum dan sesudah dilakukan ovariohisterektomi. Penelitian ini menggunakan tiga ekor anjing betina lokal dengan kisaran umur 5-7 bulan. Teknik perlakuan diambil  gambar radiografi sebelum dilakukan ovariohisterektomi selanjutnya semua hewan dilakukan ovariohisterektomi melalui linea alba, setelah itu hewan di ovariohisterektomi. Pada minggu 1,2,3, dan 4 setelah di ovariohisterektomi dilakukan pengambilan gambar radiografi dan pengukuran densitas tulang. Hasil penelitian menunjukkan bahwa rata-rata densitas tulang pada minggu 0 sebelum ovariohisterektomi adalah 157.3661667 ± 23.3043699, hasil ini tidak berbeda nyata dengan hasil rata-rata densitas pada minggu 1,3, dan 4 yaitu 156.8621982 ± 12.07528008, 153.8854886 ± 15.93987235, dan 156.1769175 ± 19.84084989, perbedaan terjadi pada minggu ke 2 dengan hasil 144.6901974 ±10.76999831. Berdasarkan hasil penelitian dapat disimpulkan  bahwa penurunan densitas radiografi tulang humerus hanya bersifat sementara pada minggu ke 2 terjadi penurunan kemudian meningkat kembali pada minggu ke 3 dan minggu ke 4.ABSTRACTThe aim of this study was to obtain a picture of radiographic density of humerus bone in local dog (Canis lupus familiaris) before and after ovarianohysterectomy. This study used three local female dogs with the age range of 5-7 months. The technique of treatment is taken radiographic images before the next ovarianohysterectomy is done all animals are done ovariohisterektomi through linea alba, after which the animal in ovariohisterektomi. At weeks 1,2,3 and 4 after ovarianohysterectomy, radiographic imaging and bone density measurements were performed. The results showed that the average bone density at week 0 before ovariohisterectomy was 157.3661667 ± 23.3043699, this result was not significantly different with the average density results at weeks 1.3 and 4, ie 156.8621982 ± 12.07528008, 153.8854886 ± 15.93987235, and 156.1769175 ± 19.84084989, the difference occurs at week 2 with the result 144.6901974 ± 10.76999831. Based on the results of this study it can be concluded that degradation of humerus bone radiography density is only temporary at 2 weeks decrease then increase again at week 3 and week 4.
Determination of Gestational Age and Observation of Kacang Goat Fetal Development during 60 Days of Pregnancy by Using Transcutaneous Ultrasonography Sayuti, Arman; Khairiah, Khairiah; Siregar, Tongku Nizwan; Melia, Juli; Syafruddin, Syafruddin; Rahmi, Erdiansyah; Herrialfian, Herrialfian; Abrar, Mahdi; Panjaitan, Budianto; Daud, Razali
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.184 KB) | DOI: 10.19087/jveteriner.2019.20.4.534

Abstract

This study was aimed to observe correlation between gestational age and fetal growth of Kacang goat for 60 days after mating with transcutaneous ultrasonography visualization. Three female goats of productive age with normal estrus cycle and one male goat were used in this study. The female goats were synchronized with double injection of prostaglandin F2á (PGF2á) intramuscularly with dosage of 1 mL for each female goat with 11 days’ time interval. Observation of oestrus was conducted using male goat after being synchronized. When the sign of estrus were prominent, the female goats were naturally mated. The result was pregnant positive for one female goat. Detection of early pregnancy was observed on the 24th days after mating, with the result of 6.77 mm length embryo by isoechogenic visualization. On 35th days of pregnancy, fetal could be seen clearly in isoechogenic to hyperechogenic visualization with head diameter and fetal length were 17.4 mm and 36.2 mm, consecutively. The observation also found the placentom. On day 45 of pregnancy, head diameter and fetal length were 21.8 mm and 40.6 mm. Later on day 49 of pregnancy, the size of gestational saccus was 44.1 mm, with head diameter of 25 mm, and average placentom diameter size of 12.4 mm. On day 53 of pregnancy, head diameter was 25.2 mm with fetal length of 63.6 mm and placentom diameter of 15 mm. On day 56 of pregnancy, vertebrae of fetal were observed using hyperechogenic visualization and placentom was measured 17.9 mm in diameter. On day 60 of pregnancy, we observed that the fetal length was 79.8 mm and the organs such as eyes, heart, liver, os costae, and fetal extremities, could be observed clearly. The size of developing fetal and organs would grow along with the addition of gestational age.
ANALISIS KADAR LEMAK SUSU KAMBING PERANAKAN ETAWA SEBELUM DAN SESUDAH DIPASTEURISASI DI PETERNAKAN LAMNYONG KOTA BANDA ACEH (Analysis Of Milk Fat Content Ettawa Before And After Pasteurization At The Farm Lamnyong Banda Aceh) Yusa, Mirza; ismail, ismail; razali, razali; ferasyi, t reza; safruddin, safruddin; panjaitan, budianto
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 1 (2017): NOVEMBER - JANUARI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (279.71 KB) | DOI: 10.21157/jim vet..v2i1.5887

Abstract

ABSTRAKSusu kambing segar bersifat mudah rusak dan memerlukan penanganan pasca panen dan pengolahan yang cepat dan memadai. Pasteurisasi adalah perlakuan yang diberikan terhadap susu segar supaya tidak cepat rusak. Penelitian ini bertujuan untuk mengetahui dan memberikan informasi kepada masyarakat tentang kadar lemak susu  kambing peranakan Etawa di peternakan Lamnyong Kota Banda Aceh sebelum dan sesudah dipasterusisasi. Penelitian ini dilakukan dengan metode Gerber dengan pasteurisasi suhu rendah waktu lama (low temperature long time /LTLT = 62 0C – 65 0C ). Penelitian ini dilakukan dengan analisis deskriptif dengan masing-masing 6 kali ulangan sehingga mendapatkan hasil yang akurat. Berdasarkan hasil penelitian memperlihatkan kadar lemak susu segar sebelum dan sesudah dipasteurisasi terdapat perbedaan. Susu yang diberi perlakuan pasteurisasi memiliki kadar lemak 5% dan susu tidak diberi perlakuan atau tidak dipasteurisasi memiliki kadar lemak 4,5%. Hal ini terjadi karena setelah dilakukan pasteurisasi susu terjadi penguapan komponen-komponenya sehingga kadar lemak susu cenderung meningkat. ABSTRACTFresh goat's milk is easily damaged and requires quick and adequate post-harvest handling and processing. Pasteurization is the treatment given to fresh milk so as not to be damaged quickly. This study aims to find out and provide information to the public about the fat content of goat milk Ettawa breeding Lamnyong Banda Aceh before and after the pasteurized. This research was done by Gerber method and using pasteurization method with low temperature long time (LTLT = 62 0C - 65 0C).This research was conducted with descriptive analysis with 6 replication so that get accurate result. Based on the results of the study showed that fresh milk fat content before and after dipasteurisasi there is a difference Milk treated with pasteurization has 5% fat content and milk is not treated or not pasteurized has a fat content of 4.5%. This happens because after milk pasteurization occurs evaporation of its components so that milk fat content tends to increase.
PENGARUH DEPOSISI SEMEN SAAT INSEMINASI BUATAN TERHADAP ANGKA KEBUNTINGAN SAPI (The Effect of Semen Deposition During Artificial Insemination on Pregnancy Rate in Cows) dana, wanti dessi; hamdan, hamdan; panjaitan, budianto; riady, ginta; wahyuni, sri; iskandar, cut dahlia
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (218.643 KB) | DOI: 10.21157/jim vet..v1i4.4812

Abstract

ABSTRAKPenelitian ini bertujuan mengetahui pengaruh deposisi semen saat inseminasi buatan terhadap angka kebuntingan sapi. Responden dalam penelitian ini adalah petugas inseminator profesional (bersertifikat) yang bertugas di Kecamatan Kuta Cot Glie, Krueng Barona Jaya, dan Blang Bintang. Jumlah sampel responden untuk angka kebuntingan pada penelitian ini adalah sapi-sapi betina yang ada di tiga kecamatan di Aceh Besar tersebut yang siap untuk diinseminasi selama bulan April sampai Mei 2017. Hasil dari penelitian ini dianalisis secara deskriptif. Hasil dari penelitian ini menunjukkan deposisi semen pada cincin serviks ketiga lebih rendah dibandingkan dengan deposisi semen pada cincin serviks keempat dengan persentase non return rate (60-90 hari) pada responden  I, II dan III secara berturut-turut adalah 80,56% ; 87,64%  dan 94,55%. Berdasarkan hasil penelitian dapat diambil kesimpulan bahwa angka kebuntingan pada deposisi semen cincin serviks keempat lebih tinggi dari pada deposisi semen pada cincin serviks ketiga. ABSTRACTThe aims of study was to determine the effect of semen deposition during artificial insemination on pregnancy rate cow. Respondents in this research are professional inseminator (certified) from Kecamatan Kuta Cot Glie, Krueng Barona Jaya and Blang Bintang. The number of respondent samples for pregnancy rate in this study were female cows in three subdistricts in Aceh Besar that available for artifial inseminated during April and May 2017. The data of this study were analyzed descriptively. The results of this study indicated that the position at the fourth cervical ring of semen deposition has the higher pregnancy rate with the percentage of NRR (60-90 days) on the respondent I, II and III respectively is 80.56%; 87.64% and 94.55%. Based on the results of the research can be concluded that pregnancy rate on the fourth cervical ring semen deposition better was than semen deposition on the third cervical ring semen deposition.
DENSITAS RADIOGRAFI TULANG FEMUR ANJING LOKAL (Canis lupus familiaris) YANG DIOVARIOHISTEREKTOMI (Radiography Density of Femur Bone On Lokal Dogs (Canis lupus familiaris) That In Ovariohisterektomi) Sikumbang, Dinda Jasmal; Panjaitan, Budianto; Syafruddin, Syafruddin; Erwin, Erwin; Masyitha, Dian; Hamdan, Hamdan
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 3 (2018): MEI - JULI
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (395.32 KB) | DOI: 10.21157/jim vet..v2i3.7815

Abstract

Penelitian ini bertujuan untuk mendapatkan gambaran densitas radiografi tulang femur pada anjing lokal. Hewan yang digunakan dalam penelitian ini adalah 3 ekor anjing betina lokal dengan kisaran umur 5-7 bulan. Pengambilan gambar radiografi dan pengukuran densitas radiografi tulang femur dengan menggunakan Software Image J pada minggu ke 0 (sebelum diovariohisterektomi), minggu ke 1, 2, 3, dan 4 (setelah diovariohisterektomi). Hasil penelitian menunjukkan bahwa rata-rata densitas radiografi tulang femur sebelum diovariohisterektomi adalah 151,8401±5,8767. Hasil ini menunjukkan berbeda nyata  pada  minggu 1, 2, dan 4 setelah diovariohisterektomi dengan hasil densitas radiografi tulang 168,7952±7,88446, 181,0557±7,81876, dan 169,1759±4,73123. Pada minggu 3 setelah diovariohisterektomi menunjukkan hasil tidak berbeda nyata dengan densitas radiografi tulang sebelum diovariohisterektomi yaitu 152,9360±7,38131. Berdasarkan hasil penelitian dapat disimpulkan bahwa densitas tulang dari hasil pengambilan gambar radiografi tulang femur anjing lokal (Canis lupus familiaris) didapatkan terjadi peningkatan densitas radiografi pada minggu ke 1, 2, 4 dan menurun pada minggu 3. Penurunan dan peningkatan densitas radiografi pada tulang femur bersifat fluktuasi. (This study aimed  to get radiodensity of femoral bone in local dog. Animals used in this study were 3 local female dogs with the age range of 5-7 months. Radiographic imaging and radiographic density measurements of femur bone using Software Image J before and after ovariohysterectomy at weeks 1, 2, 3, and 4. The results show that the average radiodensity of femur bone before ovariohysterectomy was 151,8401± 5,8767. This result had significantly different with 1, 2, and 4 weeks after ovariohysterectomy. In week 3 after ovariohysterectomy, the results were not significantly different from the radiograph density of the femur bone before in ovariohysterectomy that was 152.9360 ± 7.38131. Based on the results of the study it can be concluded that bone density resulting from radiography imaging of local dog's femur (Canis lupus familiaris) using Image Software Image J obtained  radiographic density increased at week 1, 2, 4 and decreased at week 3. The decrease and increase of radiographic density of the femur bone is a fluctuation).
Gambaran histologi ovarium sapi aceh pascavitrifikasi menggunakan dimetyl sulfoksida dengan konsentrasi berbeda Syafruddin, .; Sayuti, Arman; Sumardi, Rahmat Aditya; Panjaitan, Budianto
ARSHI Veterinary Letters Vol. 2 No. 4 (2018): ARSHI Veterinary Letters - November 2018
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (333.431 KB) | DOI: 10.29244/avl.2.4.77-78

Abstract

This study aims to determine the morphology of Aceh bovine ovarian pasca vitrification, after its been exposed to dimetyl sulfoksida (DMSO) cryoprotectant. This study use a completely randomized design of one-way pattern ANOVA acquire three replications. Ovaries used in this study are Aceh cattle ovarian which collected from slaughter house (RPH) accounted as 4 organs, to the next sliced into 9 pieces. Ovarian pieces are then grouped into 3 treatment groups namely ovaries which exposed into solution containing DMSO 10% (P1), 20% (P2), and 30% (P3). The results showed that the average number of normal follicles is highest at P1 41,33 ± 32,51; followed by P2 20,00 ± 16,09 , and P3 15,66 ± 10,50. It was concluded that  the ovarian tissue was exposed with DMSO 10% was able to maintain of the normal follicle than DMSO 20% and 30% although statistical test results showed no significant difference between group (P>0,05).
Gambaran histologis folikel ovarium sapi aceh pascavitrifikasi menggunakan etilen glikol Sayuti, Arman; Hidayah, Jamilatun; Akmal, Muslim; Panjaitan, Budianto
ARSHI Veterinary Letters Vol. 3 No. 1 (2019): ARSHI Veterinary Letters - Februari 2019
Publisher : Faculty of Veterinary Medicine, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.688 KB) | DOI: 10.29244/avl.3.1.3-4

Abstract

Penelitian ini bertujuan mengetahui gambaran histologis folikel ovarium sapi aceh pasca vitrifikasi menggunakan etilen glikol (EG) dengan berbagai konsentrasi berbeda yaitu 10%, 20% dan 30%. Phosphate buffered saline (PBS), 0.25 M sukrosa, 0.5 M sukrosa dan krioprotektan EG digunakan sebagai larutan vitrifikasi. Ovarium dipaparkan dengan larutan mengandung krioprotektan EG konsentrasi 10%, 20%, dan 30% selama 5 menit masing-masing pada suhu kamar, dikemas dalam straw, lalu divitrifikasi dalam nitrogen cair (-196 ⸰C), dan thawing dalam air pada 37 ⸰C. Kondisi normal dan keutuhan folikel pascavitrifikasi diamati secara histologi. Hasil pengamatan menunjukkan bahwa ovarium setelah vitrifikasi dengan EG 10% dan 20% memperlihatkan presentase keutuhan folikel 32.83% dan 45.04%, sedangkan EG 30% memperlihatkan presentase tertinggi yaitu 54.96%. Dari hasil penelitian ini dapat disimpulkan bahwa EG 30% lebih baik dalam mempertahankan keutuhan folikel ovarium sapi aceh dibandingkan dengan EG 10%  dan 20%.
THE IMAGE OF EMBRYO AND FETAL OF ETAWA CROSSBRED GOATS USING ULTRASONOGRAPHY AT DIFFERENT GESTATIONAL AGES Melia, Juli; Fauziah, Fauziah; Hamdan, Hamdan; Panjaitan, Budianto; Syafruddin, Syafruddin; Armansyah, Teuku; Siregar, Tongku Nizwan
Jurnal Kedokteran Hewan Vol. 12 No. 2: June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v12i2.3768

Abstract

This study aimed to estimate the gestational age of Etawa crossbred goats based on ultrasound images of embryo and fetal usingtranscutaneous ultrasonography. Etawa crossbred does (n= 5) were synchronized using double PGF2α injection with 10 days interval and matednaturally. Early pregnancy was diagnosed on 20th day after mating base on isoechogenic image of the embryo (2.65±0.75 mm) andhypoechogenic appearance of gestational sac. On 30th day of the first trimester, the embryo size was 8.31±0.42 mm. The embryo reached10.44±0.51 mm on 35th day. On 60th day, the existence of the fetal were clearly appeared along with isoechogenic of umbilical cord,hyperechogenic os cranium (25.5±0.1 mm), and body length (51.83±1.59 mm). On 75th day, the diameter of the os cranium was 33.03±0.20 mm,body length of 110±1.73 mm, and bones structures appeared hyperechogenic for os thoracalis and os vertebrae. The placenta was isoechogenoicwith diameter of 23.3±0.2 mm. On 90th day, the placenta dominated the uterus (22.67±1.70 mm) and reached its maximum diameter on 145th day(33.73±2.91 mm). In conclusion, early pregnancy detection of Etawa crossbred goats using transcutaneous ultrasonography could be performedon 20th day after mating
Effect of Storage Time of Epididymis at 5 oC on Spermatozoa Quality of Aceh Local Goat Hamdan, Hamdan; Panjaitan, Budianto; Sutriana, Amalia; Aliza, Dwinna; Rahmi, Erdiansyah
Jurnal Kedokteran Hewan Vol 4, No 2 (2010): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v4i2.9811

Abstract

The objective of this study was to evaluate the effect of storage time of epididymis at 5 oC on spermatozoa quality of Aceh local goat. This research was conducted at Reproduction Laboratory, FKH Unsyiah from May to June 2009. Nine epididymis from local goat at the age of 1.5-2.0 years were used in this study. The epididymis were collected from abattoir in Banda Aceh. The experimental design used in this study was completely randomized design with 3 treatments and 3 replications. Spermatozoa quality which consist of sperm concentration, motility, amount of life sperm, and sperm abnormality were examined after the collection of epididymis on day 0 (H-0), day 1 (H-1), and day 3 (H-3) post storage at 5 oC. Data were analyzed using one-way analyses of variance (ANOVA). The result showed that no significant difference (P 0.05) seen in the spermatozoa quality after different storage time. The average of spermatozoa concentration on H-0, H-1, and H-3 were 318x107/ml, 282x107/ml, and 241x107/ml respectively. On the average, the percentage of spermatozoa motility on H-0, H-1, and H-3 were 82,27±2,75; 80,25±2,83; and 78,07±0,92%, respectively. Life spermatozoa observed on H-0, H-1, and H-3 were 82,29±2,71; 80,63±1,87; and 80,09±3,31%, respectively. Observation on spermatozoa abnormality showed that the average of spermatozoa abnormality on H-0, H-1, and H-3 were 7,23±0,27; 8,21±0,55; and 10,75±3,14%, respectively. It could be concluded that the spermatozoa quality were not affected by the storage time at temperature of 5 oC.