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The Roles of Nitrogen and Potassium to Phosphoenolpyruvate Carboxylase Activity in the Leaves of Ratoon Crop M 442-51 and PS 60 Sugarcane Varieties Hadisaputro, Suyoto; Rochiman, Kusriningrum; PDN, Mirzawan; Sukarso, Gunawan; Sugiharto, Bambang
Jurnal ILMU DASAR Vol 9 No 1 (2008)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

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Abstract

Study on the roles of nitrogen and potassium to phosphoenolpyruvate carboxylase (PEP-Case) activity in the leaves of ratoon crop of M 442-51 and PS 60 cane varieties were conducted in the Indonesian Sugar Research Institute (ISRI) Pasuruan, East Java. The experiment was arranged in a factorial trial using a completely ran-domized block design with three replicates. The factors used as a treatments in this experiment were: (1) sugar-cane variety, i.e. M 442-51 (V1) and PS 60 (V2), (2) plant category, i.e. plant crop (PC) and second ratoon crop (RC2), (3) dosage of nitrogen, i.e. 1 q AS/ha (N1) and 8 q AS/ha (N2), and (4) dosage of potassium, i.e. 1 q KCl/ha (K1) and 5 q KCl/ha (K2). PEP-Case activity variable was observed on 0, 1 and 4 weeks after treatment (wat). The results of experiment showed that nitrogen (N) and potassium (K) nutrients as single or mixture treatments were increasing the activity level of PEP-Case in the cane leaves significantly. There was indication that the effect of N is stronger than that the effect of K to PEP-Case activity in the leaves of cane. On the other hand, it was found that the activity of PEP-Case in M 442-51 was higher than that on PS 60. However, when the availability of both nutrients was limited, the decreasing activity of PEP-Case on PS 60 was sharper than that found on M 442-51. Study on plant category showed that the activity of PEP-Case on PC was higher than that found on RC2. Reducing of PEP-Case activity on RC2 was predicted as one factor that caused reducing of RC2 productivity. Base on these findings, it can be concluded that PEP-Case activity model in the leaves could be used as an early characteristic of cane ratooring ability. There were indications that the ability of both Mn and Cu nutrients affected the increasing of PEP-Case activity better than that influenced by N and K nutrients, but it needs further investigation. 
RESPONS EMBRIOGENESIS MIKROSPORA TANAMAN TEBU (Saccharum spp.) PADA SUHU DAN LAMA INKUBASI CABANG MALAI DI DALAM MEDIUM B Suaib, Suaib; Mangoendidjojo, W.; PDN, Mirzawan; Indrianto, A.
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 14 No 1 (2008): December 2008
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/310

Abstract

Microspore embryogenesis under different temperature and time duration pretreatments of spike segments of Saccharum spp., clone PS862 has been studied in in-vitro laboratory of Faculty of Biology Gadjah Mada University, Yogyakarta. Combination pretreatments of a 4 and 34° C of temperatures, and 0, 2, 4, and 7 days duration of incubation of spike segments in the B medium were studied in this observation. A B5 medium of Gamborg supplemented with 2 mgl-1 2.4-D or 2 mg.l-1 NAA was also studied on the formation of proembryos and embryos under 25°C temperature in the dark for at least 30 days. Results of the research showed that the percentage of viable microspores was decreased as simultaneously prolonged of the time of duration in the B medium either under low temperature or under high temperature pretreatments tested. Incubation of spike segments under low temperature after 4 days was decreased the percentage of viable microspores over 44%. In contrast, under high temperature pretreatment the percentage of viable microspores was decreased over 54%. The higher percentage (12.5%) of binucleate microspores was achieved after 7 days incubation of spike segments in the B medium under the low temperature compared to the high temperature (7.14%). Besides, the higher percentage (87.5%) of simmetrical binucleate microspores as proembryo and embryo precursor was obtained after 2 days incubation in the B medium under the low temperature compared to the high temperature (79.0%). Finally, only B5 medium supplemented with 2 mgl-1 of NAA was yielded the proembryos and embryos after 30 days of incubation.