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Crude Palm Oil Consumption As A Hepatoprotectors To Housewives In Bogor Perdani, Claudia Gadizza; Zakaria, Fransiska R; Prangdimurti, Endang
Jurnal Teknologi Pertanian Vol 17, No 2 (2016)
Publisher : Fakultas Teknologi Pertanian Universitas Brawijaya

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Abstract

Kekurangan Vitamin A (KVA) di Indonesia masih merupakan masalah utama. Kekurangan vitamin A tingkat berat (rabun senja) telah jarang ditemui, tetapi tingkat KVA subklinis masih bisa ditemukan. Minyak sawit mentah (CPO) memiliki kandungan tinggi karotenoid sebagai sumber provitamin A. CPO secara alami berwarna merah karena mengandung karotenoid yang tinggi, dan belum dimanfaatkan di Indonesia. CPO mengandung karotenoid dan vitamin E yang berfungsi sebagai antioksidan bagi tubuh. Antioksidan dapat mengurangi kerusakan sel dengan menetralisir radikal bebas serta mencegah kerusakan lipid, protein, enzim, dan DNA. Tujuh puluh responden dipilih untuk konsumsi CPO selama 2 bulan dengan dosis ± 3.27 ml/hari. Plasma darah dari 22 responden yang sehat dianalisis dengan metode asam Tri Fluoro asetat (TFA), enzim aspartat Transaminase (AST), Alanine transaminase (ALT), Alkaline Phospatase (ALP) kit. Hasil penelitian menunjukkan 16 responden mengalami peningkatan jumlah retinol plasma dan penurunan tingkat aktivitas AST, ALT, dan enzim ALP. Penelitian ini menunjukkan bahwa CPO memiliki aktivitas antioksidan yang dapat meningkatkan kesehatan hati, sehingga dapat menjadi alternatif sumber provitamin A untuk menangani kekurangan vitamin A di Indonesia
Peluang Aplikasi Mikroenkapsulat Vitamin A Dan Zat Besi Sebagai Fortifikan (The Chance of Aplication Microencapsulat Vitamin A and Iron as Fortificants) Sugiyono, Sugiyono; Asterini, Windi; Prangdimurti, Endang
JURNAL PANGAN Vol 25, No 1 (2016): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1392.839 KB) | DOI: 10.33964/jp.v25i1.306

Abstract

Vitamin A dan zat besi termasuk salah satu zat gizi mikro yang dibutuhkan oleh tubuh. Kekurangan asupan dan absorbsi zat gizi mikro dapat mengakibatkan gangguan pada kesehatan, pertumbuhan, dan fungsi lainnya di dalam tubuh. Program fortifikasi merupakan salah satu cara yang dilakukan oleh pemerintah untuk mengatasi masalah defisiensi vitamin A dan zat besi. Kedua mikronutrien ini sering digunakan menjadi fortifikan akan tetapi masing-masing senyawa ini memiliki reaksi negatif di dalam bahan pangan. Vitamin A merupakan senyawa yang rentan terhadap suhu tinggi, cahaya dan udara (oksigen), sedangkan zat besi dapat menghasilkan efek negatif pada sensori (bau dan warna) pangan fortifikasi. Perlindungan fortifikan dari pengaruh lingkungan sangat diperlukan untuk mendukung keberhasilan program fortifikasi. Salah satu caranya ialah dengan menggunakan teknologi enkapsulasi. Berbagai penelitian menunjukkan bahwa fortifikasi dengan menggunakan teknik mikroenkapsulasi menghasilkan fortifikasi yang lebih stabil, tidak merubah bahan pangan pembawa fortifikan secara fisik dan kimia. Oleh karena itu pembuatan mikroenkapsulasi fortifikan dinilai lebih efisien dan efektif dalam mengatasi masalah defisiensi zat gizi mikro.Vitamin A and iron are essential micronutrients needed by the body. Deficiency of intake and absorption of micronutrients can lead to disturbances in health, growth and other functions in the body. Fortification is one of the government programs to cope with the deficiency of vitamin A and iron. Both compounds are often used as fortificants, but their present promotes undesirable reaction in foodstuffs. Vitamin A is susceptible to high temperature, light and air (oxygen), while iron can result in detrimental effects on the color and smell. Therefore, the protection of fortificants against environmental effect in food system is highly required, and encapsulation is a promising technique. Previous studies showed that microencapsulation technique produced more stable compounds and unchanged chemical and physical characteristics of fortified food. For this reason, microencapsulation in fortified food is considered as efficient and effective way in addressing micronutrient deficiencies. 
Diet Berbasis Sorgum (Sorghum bicolor L Moench) Memperbaiki Proliferasi Limfosit Limfa dan Kapasitas Antioksidan Hati Tikus Zakaria, Fransiska R.; Prangdimurti, Endang; Puspawati, G. A. Kadek Diah; Thahir, Ridwan; Suismono, Suismono
JURNAL PANGAN Vol 20, No 3 (2011): PANGAN
Publisher : Perum BULOG

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1933.278 KB) | DOI: 10.33964/jp.v20i3.155

Abstract

Sorgum merupakan tanaman serealia yang sangat berguna sebagai sumber karbohidrat alternatif dalam program diversifikasi pangan. Sorgum memiliki kandungan protein yang lebih tinggi dibandingkan beras dan berprospek baik untuk dikembangkan di Indonesia. Banyak penelitian telah menunjukkan bahwa sorgum sangat baik untuk kesehatan, antara lain untuk mengurangi resiko penyakit degeneratif. Penelitian secara in vitro sebelumnya, menunjukkan bahwa serealia ini mampu meningkatkan proliferasi limfosit manusia, yang menunjukkan perbaikan sistem imun. Tujuan penelitian ini adalah untuk mempelajari efek sorgum terhadap sistem imun dan kapasitas antioksidan secara in vivo pada tikus. Tiga kelompok tikus diberi pakan kontrol, pakan mengandung 50 persen atau 100 persen sorgum sebagai sumber karbohidrat selama 7 minggu. Hasil penelitian menunjukkan bahwa kelompok tikus yang diberi pakan mengandung 50 persen atau 100 persen sorgum mengalami peningkatan aktivitas proliferasi mencapai berturut-turut 70 persen dan 63 persen, aktivitas antioksidan hati (DPPH) mencapai 38 persen dan 29 persen, aktivitas enzim superoksida dismutase (SOD) mencapai 98 persen dan 91 persen, aktivitas enzim katalase (CAT) mencapai 28 persen dan 21 persen, dan aktivitas glutation peroksida (GPx) mencapai 57 persen dan 33 persen, akan tetapi mengalami penurunan kandungan malondialdehid (MDA) hingga 22 persen dan 16 persen. Penelitian ini menunjukkan bahwa sorgum mempunyai aktifitas imunostimulani dan aktivitas perbaikan antioksidan sehingga baik bagi kesehatan.Sorghum is a cereal that would be useful as alternative carbohydrate source in food diversification program. It has higher protein content than rice and good prospect to be developed in Indonesia. Researches have shown that sorghum has functions in health, such as to decrease degenerative disease risk. Previous in vitro study of sorghum showed that this cereal could increase human lymphocyte cell proliferation in vitro, indicating immune system improvement. The objectives of this research were to study the effects of sorghum on the in vivo immune system and liver antioxidant capacity in rats. Three groups of rats were fed control diet, diet containing 50 percent or 100 percent sorghum as sources of carbohydrate. The results showed that the rats fed with 50 percent or 100 percent sorghum displayed increase in, respectively, proliferation activity by 70 percent and 63 percent; liver antioxidant activity (DPPH) by 38 percent and 29 percent, super dioxide dismutase enzyme activity (SOD) by 98 percent and 91, catalyst enzyme activity (CAT) by 28 percent and 21 percent, and glutathione peroxides enzyme activity (GPx) by 57 percent and 33 percent; but decreased in malondialdehyde (MDA) by 22 percent and 16 percent. This research showed that sorghum has immunostimulation and antioxidant improvement activities and will be very good as source of carbohydrate diet. 
POTENSI ANTIOKSIDAN GEL DAN DAUN KACAPIRING (Gardenia jasminoides Ellis) Widnyana Yoga, IB Ketut; Andarwulan, Nuri; Prangdimurti, Endang
Prosiding Seminar Nasional MIPA 2011: PROSIDING SEMINAR NASIONAL MIPA UNDIKSHA 2011
Publisher : Prosiding Seminar Nasional MIPA

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Abstract

Abstract: Leaf of kacapiring (Gardenia jasminoides Ellis) is a part of plant which is having component that can form gel. It has chlorophyll pigment and phenolic compound. The aims of this research were to identificate bioactive compound in leaves and gel which has antioxidant potency. Chlorophyll total was analyzed by spectrophotometer and its derivations of aceton extract (99.9%) by thin layer chromatography (TLC), meanwhile phenolic total and antioxidant capacity were analyzed by spectrophotometer. The result showed that bioactive component of kacapiring leaves and gel were chlorophyll total of 4926.25+190.31 and 1166.86+8.73  mgKg-1db. Both of them had 5 fractions by acetone extract, i.e. chlorophyll a, chlorophyll b, lutein (chlorophyll derivates), feofitine and carotene. Phenolic total in leaves and gel contained 5215.91+2.97 and 2648.16+56.22 GAE/g db, and antioxidant capacity had 1.5 x 10-1+0.00 and 3.1x10-3 +0.00 mM TEAC/mg dw respectively
COMPARISON OF SEVERAL PROCESSING METHODS IN PRESERVING THE FLAVOR PROPERTIES OF ANDALIMAN (ZANTHOXYLUM ACANTHOPODIUM DC.) FRUIT Napitupulu, Florensia Irena R.; Wijaya, Christofora Hanny; Sulistiyani, S.; Prangdimurti, Endang; Akyla, Clarissa; Yakhin, Lisa Amanda; Indriyani, Susi
Journal of Engineering and Technological Sciences Vol 52, No 3 (2020)
Publisher : Institute for Research and Community Services, Institut Teknologi Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.eng.technol.sci.2020.52.3.7

Abstract

Andaliman (Zanthoxylum acanthopodium DC.) is a plant endemic to North Sumatra. Its fruit has a very specific citrus-like aroma, gives a tingling sensation and is commonly used for seasoning in Bataknese traditional cuisine. To extend the shelf life and preserve the quality of andaliman fruit, post-harvest handling is needed. Seven drying methods were applied and compared, i.e. sun, air, fluidized bed, oven, far infrared, freeze, and spray drying. Considering the physicochemical and sensory properties and efficiency, oven drying was selected for further study. The optimum condition for drying was temperature at 54 °C for 8 h resulting in 0.67 desirability level based on the response surface method (RSM). Spray drying encapsulation of andaliman fruit extract was performed at an inlet temperature of 150 °C with an extract to carrier ratio of 1:8. Maltodextrin (MD) and gum arabic (GA) with a ratio of 3:2 was chosen as the carrier agent. The quantitative descriptive analysis (QDA) result showed that the encapsulated andaliman powder had a flavor that resembled fresh andaliman with a more pronounced taste, a citrus-like trigeminal sensation, and a sour floral citrusy aroma.
Upaya Penghilangan Rasa Pahit Tepung Biji Kelor (Moringa oleifera) dan Aplikasinya untuk Pangan Fungsional Muhammad Iqbal Fanani Gunawan; Endang Prangdimurti; Tjahja Muhandri
Jurnal Ilmu Pertanian Indonesia Vol. 25 No. 4 (2020): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.25.4.636

Abstract

Moringa oleifera is a plant that has many benefits in each part. Moringa seeds have high nutrient content but they have not been used as food ingredients because it contains cyanigenic glycoside and a bitter taste. This study aims to obtain the best method in eliminating the bitter taste and cyanide of moringa seeds, analyzing its proximate nutritional levels and antioxidant capacity, and the level of acceptance when it’s applied to cookie products. This study used different types of soaking solution (water, 3.5% NaCl, 1N CH3COOH solution, and 0.5% NaHCO3) and boiling temperature (70°C, boiling water temperature (± 97°C), and pressure boiling (±115°C)). Flour with NaHCO3 immersion treatment continued by pressurized boiling for 35 minutes was chosen to be the best flour because it had the lowest cyanide content, reduced bitter taste, and the highest FRAP (Ferric Reducing Antioxidant Power) values (33.40±1.22 mg/kg; 3.40±1.24 mg/kg; and 58.70±2.67 mg AEAC/g) compared with untreated moringa seed flour (152.15±0.95 mg/kg ;10.29±2.92 mg/kg; and 17.43±1.22 mg AEAC/g). The selected flour was used as a substitute for flour (0%, 10%, 20%, and 30%) in cookies making. Sensory hedonic rating analysis on cookies used 60 untrained panelists. Substitution up to 30% overall did not have a significant difference in acceptance compared to control (0%) except for color attribute. Keywords: bitter, cyanide, Moringa oleifera, functional food, moringa seed flour
Karakteristik dan Evaluasi Prebiotik Tepung Biji Cempedak (Artocarpus champeden) Ummul Khayrah; Endang Prangdimurti; Lilis Nuraida
Jurnal Ilmu Pertanian Indonesia Vol. 27 No. 2 (2022): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.27.2.248

Abstract

This study aims to carry out chemical characterization and evaluate the prebiotic potential of cempedak (Arthocarpun champeden) seed flour. Cempedak seeds were processed by boiling and roasting, then dried and grinded. Boiled cempedak seed flour (BCF), roasted cempedak flour (RCF), and untreated cempedak seed flour (UCF) were analyzed proximately and measured the profiles of total starch and undigestable carbohydrates, which included resistant starch, total dietary fiber, insoluble dietary fiber, and soluble dietary fiber. Resistant starch content increased significantly (p<0.05) from 8% in UCF to 19% in BCF and RCF. Boiling and roasting treatments caused a decrease in SDF levels and an increase in IDF levels. The resistance of starch to hydrolysis by artificial gastric acid decreased after boiling. The value of prebiotic effect (PE) and prebiotic index (PI) showed a positive value of UCF on the growth of L. plantarum 1-S27202 (PE 0.27; PI 0.68) and the BCF on the growth of L. rhamnosus R23 (PE 0.18; PI 0.44). Keywords: Artocarpus champeden, chempedak seeds, resistant starch, dietary fiber, prebiotic
AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN SUJI (Pleomele angustifolia N.E. Brown) [Antioxidant Activity of Suji (Pleomele angustifolia N.E. Brown) Leaf Extract] Endang Prangdimurti; Deddy Muchtadi; Made Astawan; Fransiska R Zakaria
Jurnal Teknologi dan Industri Pangan Vol. 17 No. 2 (2006): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

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Abstract

Numerous studies showed that chlorophyll and its derivatives had antioxidant activity. This research was conducted to obtain the chlorophyll-rich suji leaf liquid extract (SLE) and then to investigate the oral administration this extract on the antioxidant status by means of animal study. The use of Tween 80 0.75%  in sodium citrate 12 mM solution as an extraction solution combined with 30 minutes incubation time at 70-75oC increased significantly the total chlorophylls content, the water-soluble chlorophylls content and the antioxidant activity of the yielded extract, compared to the suji leaf water-only extract.  Two-month oral administration of SLE  to male Sprague Dawley rats showed that the liver MDA level significantly decreased, and the liver catalase and superoxide dismutase antioxidant enzyme activities significantly increased compared to the control group. It was suggested that the chlorophyll content in SLE might increase the antioxidant status of animal tested.
EVALUASI IN VITRO TERHADAP KEMAMPUAN ISOLAT BAKTERI ASAM LAKTAT ASAL AIR SUSU IBU UNTUK MENGASIMILASI KOLESTEROL DAN MENDEKONJUGASI GARAM EMPEDU [In Vitro Evaluation of Cholesterol Assimilation and Bile Salt Deconjugation by Lactic Acid Bacteria Isolated Lilis - Nuraida; Siti - Winarti; Hana - -; Endang - Prangdimurti
Jurnal Teknologi dan Industri Pangan Vol. 22 No. 1 (2011): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.37 KB)

Abstract

Hypercholesterolemia is a risk factor for cardiovascular disease, the leading cause of death in many countries. Several studies have shown that reduction of excessive levels of cholesterol in the blood decreases the risk of cardiovascular disease. It is therefore important to develop ways of reducing serum cholesterol. Based on in vitro and in vivo studies, some of lactic acid bacteria (LAB) having potential probiotic properties can reduce total cholesterol and low-density lipoprotein cholesterol levels. The aim of this study was to evaluate the ability of LAB isolated from breast milk in reducing cholesterol by assimilation and by bile salt deconjugation activity in vitro.Thirteen strains of LABs were evaluated for their acid and bile salt resistance and selected to test their ability to assimilate cholesterol and to deconjugate bile salt (natrium taurocholate) in vitro. Cholesterol assimilation activity was determined by measuring the difference between the remaining cholesterol in broth medium inoculated with LAB with cholesterol in control after incubation. Bile salt deconjugation activity was determined by measuring free cholic acid released in broth medium after incubation with LAB. The results shows that most of the isolates was susceptible to low pH and all isolates used were able to survive in the presence of 0.5% bile salt. The LAB were also able to assimilate cholesterol at varying levels ranging from 0.86-14.97 µg/ml, with the highest activity showed by Pediococcus pentosaceus 1-A38, Pediococcus pentosaceus 2-B2 and Pediococcus pentosaceus 2-A16. Taurocholate deconjugation assay showed that the isolates have weak bile salts deconjugation activity as indicated by free cholic acid released ranging from 0.06-0.25 µmol/ml, with the highest release in Pediococcus pentosaceus 1-A38 and Pediococcus pentosaceus 1-A22. The present study suggest that Pediococcus pentosaceus 1-A38 was potential for the development of probiotic products with specific benefit to reduce cholesterol through cholesterol assimilation and deconjugation of bile salt.
POTENSI LACTOBACILLUS YANG DIISOLASI DARI AIR SUSU IBU UNTUK MENCEGAH DIARE [Potential of Lactobacillus Isolated from Breast Milk to Prevent Diarrheae] Lilis Nuraida; . Hana; Apriliana W. Hartanti; Endang Prangdimurti
Jurnal Teknologi dan Industri Pangan Vol. 23 No. 2 (2012): Jurnal Teknologi dan Industri Pangan
Publisher : Departemen Ilmu dan Teknologi Pangan, IPB Indonesia bekerjasama dengan PATPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (480.476 KB) | DOI: 10.6066/jtip.2012.23.2.158

Abstract

Some of Lactobacillus species isolated from breast milk are known to have antimicrobial activities, including against Escherichia coli. The aims of this study were to evaluate the antimicrobial activity of Lactobacillus species isolated from breast milk against enteropathogenic Escherichia coli strain K1.1 and the effectiveness of the Lactobacillus isolates to prevent diarrhea on rats (Sprague Dawley). The infective dose of E. coli K1.1 to induce diarrhea without causing death were also determined. Based on the antimicrobial activity there were three isolates of Lactobacillus that exhibited good inhibition againts E. coli K1.1, i.e. Lactobacillus rhamnosus R14, L. rhamnosus R23, and L. rhamnosus B16. Determination of E. coli infective dose showed that 108 CFU of E. coli K1.1 was sufficient to induce diarrhea on rat without causing death. The number of diarrhea rats and severity level in group treated with L. rhamnosus were lower than groups untreated with the Lactobacilli. This study showed that the three L. rhamnosus isolated from breast milk were able to prevent diarrhea due to infection of E. coli K1.1 when the Lactobacillus was regularly introduced prior to infection. L. rhamnosus R23 showed the best capabilities of preventing diarrhea in rats as compared to two other isolates of Lactobacillus. The incidence of diarrhea correlated with the number of lactobacilli in the feces. However when the period of diarrhea ceased, there were no difference in total lactobacilli and E. coli in the caecum, colon and feces between rats treated with L. rhamnosus and the control. This finding revealed the L. rhamnosus isolated from breast milk were potential for prevention of diarrhea when consumed regularly.